Sequence of pRL

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Sequence of pRL_TK

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Sequence of pRL-TK CXCR4 2x : 4118 BP; 1085 A; 917 C; 1058 G; 1058 T; 0 other;

BglII(1) .......... .......... .......... .......... ..........

1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct 60

1 tctagattta ctcagaagcc tggagcgccc ccggcgaatt cgccaccaat cccaaacaga 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt 120

61 ctgcgccccc ctcccccttc cttgctttgt gagagtaagc ctccgccgag ccccaaacca 120

.......... .......... .......... .......... .......... ..........

MscI(128) .......... .......... .......... .......... ..........

121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc 180

121 gaaccaccgg tgcccgtgcg tcttctcgcg gcgctaggag aattcgtggg ggggcgggag 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc 240

181 gcacctccgc ccccaaacca gccgcccacc attgaccgcc cggcgactga gcccgcccag 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ..... SmaI(284) NarI(290) .....

241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg 300

241 cgcgcggggt ctcacactgg aaaagccaga cgagcgtctg ggggcccgcc gcggcggcgc 300

.......... .......... .......... .......... .......... ..........

.......... .......... NcoI(330) .......... .......... ..........

301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg 360

301 cgccgctgcc cgagcgaccc aggatccgag gtacccctgg catatgcacc tgtccgagac 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac 420

361 ctcgtaggcg tgctgacgcc actataatgg cctctggaag acgccctgct cggcccagtg 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat 480

421 cgccgactgc gcctcgcagg caacccgctg tttgtggtcc tgccccgtgt ccatgtgata 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... PvuII(531)

481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat 540

481 gaacagtggg cctccgcgct ccctgacgtc ctcgaagtcc ctcaccgcgt cgacgaagta 540

.......... .......... .......... .......... ........ TATA_promoter

.......... .......... .......... .......... .......... ..........

541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt 600

541 ggggcaccgg gcaacgagcg caaacgaccg ccacaggggc cttctttata taaacgtaca 600

.......... ....... TATA_promoter TK_promoter ....... ..........

.......... .......... .......... .......... EcoRI(649) ..........

601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac 660

601 gaaatcaaga tactactgtg tttggggcgg gtcgcagaac agtaaccgct taagcttgtg 660

.......... .. TATA_promoter TK_promoter .. .......... ..........

.......... .......... .......... .......... .......... ..........

661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt 720

661 cgtctacgtc agccccgccg cgccagggtc caggtgaagc gtataattcc actgcgcaca 720

TATA_promoter ..... TK_promoter ......... .......... ..........

.......... .......... ......... HindIII(760) ......... ..........

721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttgattc ttctgacaca 780

721 ccggagcttg tggctcgctg ggacgtcgct gggcgaattt tcgaactaag aagactgtgt 780

......... TK_promoter ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag 840

781 tgtcagagct tgaattcgac gtcttcaacc agcactccgt gacccgtcca ttcatagttc 840

.......... .......... .......... .......... .......... chim_int

.......... .......... .......... .......... .......... ..........

841 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc 900

841 caatgttctg tccaaattcc tctggttatc tttgacccga acagctctgt ctcttctgag 900

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca 960

901 aacgcaaaga ctatccgtgg ataaccagaa tgactgtagg tgaaacggaa agagaggtgt 960

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta 1020

961 ccacaggtga gggtcaagtt aatgtcgaga attccgatct catgaattat gctgagtgat 1020

.......... .......... .......... .......... ......... T7_promoter

NheI(1024) .......... .......... .......... .......... ..........

1021 taggctagcc accatgactt cgaaagttta tgatccagaa caaaggaaac ggatgataac 1080

1021 atccgatcgg tggtactgaa gctttcaaat actaggtctt gtttcctttg cctactattg 1080

.......... .......... ......... Orf frame 2 ......... ..........

.......... .......... .......... .......... .......... ..........

1081 tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt cttgattcat ttattaatta 1140

1081 accaggcgtc accacccggt ctacatttgt ttacttacaa gaactaagta aataattaat 1140

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ttatgattca gaaaaacatg cagaaaatgc tgttattttt ttacatggta acgcggcctc 1200

1141 aatactaagt ctttttgtac gtcttttacg acaataaaaa aatgtaccat tgcgccggag 1200

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ttcttattta tggcgacatg ttgtgccaca tattgagcca gtagcgcggt gtattatacc 1260

1201 aagaataaat accgctgtac aacacggtgt ataactcggt catcgcgcca cataatatgg 1260

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 agaccttatt ggtatgggca aatcaggcaa atctggtaat ggttcttata ggttacttga 1320

1261 tctggaataa ccatacccgt ttagtccgtt tagaccatta ccaagaatat ccaatgaact 1320

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tcattacaaa tatcttactg catggtttga acttcttaat ttaccaaaga agatcatttt 1380

1321 agtaatgttt atagaatgac gtaccaaact tgaagaatta aatggtttct tctagtaaaa 1380

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tgtcggccat gattggggtg cttgtttggc atttcattat agctatgagc atcaagataa 1440

1381 acagccggta ctaaccccac gaacaaaccg taaagtaata tcgatactcg tagttctatt 1440

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gatcaaagca atagttcacg ctgaaagtgt agtagatgtg attgaatcat gggatgaatg 1500

1441 ctagtttcgt tatcaagtgc gactttcaca tcatctacac taacttagta ccctacttac 1500

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gcctgatatt gaagaagata ttgcgttgat caaatctgaa gaaggagaaa aaatggtttt 1560

1501 cggactataa cttcttctat aacgcaacta gtttagactt cttcctcttt tttaccaaaa 1560

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa atcatgagaa agttagaacc 1620

1561 cctcttattg aagaagcacc tttggtacaa cggtagtttt tagtactctt tcaatcttgg 1620

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 agaagaattt gcagcatatc ttgaaccatt caaagagaaa ggtgaagttc gtcgtccaac 1680

1621 tcttcttaaa cgtcgtatag aacttggtaa gtttctcttt ccacttcaag cagcaggttg 1680

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt aaacctgacg ttgtacaaat 1740

1681 taatagtacc ggagcacttt agggcaatca ttttccacca tttggactgc aacatgttta 1740

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tgttaggaat tataatgctt atctacgtgc aagtgatgat ttaccaaaaa tgtttattga 1800

1741 acaatcctta atattacgaa tagatgcacg ttcactacta aatggttttt acaaataact 1800

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atcggaccca ggattctttt ccaatgctat tgttgaaggt gccaagaagt ttcctaatac 1860

1801 tagcctgggt cctaagaaaa ggttacgata acaacttcca cggttcttca aaggattatg 1860

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg 1920

1861 acttaaacag tttcattttc cagaagtaaa aagcgttctt ctacgtggac tactttaccc 1920

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .... XhoI(1977) XbaI(1971)

1921 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctagactcg 1980

1921 ttttatatag tttagcaagc aactcgctca agagttttta cttgttatta agatctgagc 1980

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agccggaagt tttcacaaag ctaacaccgg aagttttcac aaagctaaca ccggatcgcg 2040

1981 tcggccttca aaagtgtttc gattgtggcc ttcaaaagtg tttcgattgt ggcctagcgc 2040

.......... .......... .......... .......... .......... ..........

ApaI(2044) NotI(2051),EagI(2051) ........ .......... ..........

2041 ggccctagag cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac 2100

2041 ccgggatctc gccggcgaag ctcgtctgta ctattctatg taactactca aacctgtttg 2100

.......... .......... .......... .... SV40_PA_terminator EBV_rev_primer

.......... .......... .......... .......... .......... ..........

2101 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt 2160

2101 gtgttgatct tacgtcactt tttttacgaa ataaacactt taaacactac gataacgaaa 2160

.......... ...... SV40_PA_terminator ...... .......... ..........

.......... .......... .......... HpaI(2194) .......... ..........

2161 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat 2220

2161 taaacattgg taatattcga cgttatttgt tcaattgttg ttgttaacgt aagtaaaata 2220

...... SV40_PA_terminator ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg 2280

2221 caaagtccaa gtccccctcc acaccctcca aaaaatttcg ttcattttgg agatgtttac 2280

.......... .......... .......... .......... .......... ..........

ClaI(2289) BamHI(2296) ........ .......... .......... ..........

2281 tggtaaaatc gataaggatc caggtggcac ttttcgggga aatgtgcgcg gaacccctat 2340

2281 accattttag ctattcctag gtccaccgtg aaaagcccct ttacacgcgc cttggggata 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 2400

2341 aacaaataaa aagatttatg taagtttata cataggcgag tactctgtta ttgggactat 2400

.......... .......... ........ AmpR_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

2401 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 2460

2401 ttacgaagtt attataactt tttccttctc atactcataa gttgtaaagg cacagcggga 2460

.......... .......... .......... .... Orf frame 2 Ampicillin ....

.......... .......... .......... .......... .......... ..........

2461 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 2520

2461 ataagggaaa aaacgccgta aaacggaagg acaaaaacga gtgggtcttt gcgaccactt 2520

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 2580

2521 tcattttcta cgacttctag tcaacccacg tgctcaccca atgtagcttg acctagagtt 2580

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 2640

2581 gtcgccattc taggaactct caaaagcggg gcttcttgca aaaggttact actcgtgaaa 2640

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 2700

2641 atttcaagac gatacaccgc gccataatag ggcataactg cggcccgttc tcgttgagcc 2700

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 2760

2701 agcggcgtat gtgataagag tcttactgaa ccaactcatg agtggtcagt gtcttttcgt 2760

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 2820

2761 agaatgccta ccgtactgtc attctcttaa tacgtcacga cggtattggt actcactatt 2820

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 2880

2821 gtgacgccgg ttgaatgaag actgttgcta gcctcctggc ttcctcgatt ggcgaaaaaa 2880

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 2940

2881 cgtgttgtac cccctagtac attgagcgga actagcaacc cttggcctcg acttacttcg 2940

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... FspI(2996)

2941 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 3000

2941 gtatggtttg ctgctcgcac tgtggtgcta cggacatcgt taccgttgtt gcaacgcgtt 3000

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 3060

3001 tgataattga ccgcttgatg aatgagatcg aagggccgtt gttaattatc tgacctacct 3060

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 3120

3061 ccgcctattt caacgtcctg gtgaagacgc gagccgggaa ggccgaccga ccaaataacg 3120

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 3180

3121 actatttaga cctcggccac tcgcacccag agcgccatag taacgtcgtg accccggtct 3180

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 3240

3181 accattcggg agggcatagc atcaatagat gtgctgcccc tcagtccgtt gatacctact 3240

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 3300

3241 tgctttatct gtctagcgac tctatccacg gagtgactaa ttcgtaacca ttgacagtct 3300

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 3360

3301 ggttcaaatg agtatatatg aaatctaact aaattttgaa gtaaaaatta aattttccta 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 3420

3361 gatccacttc taggaaaaac tattagagta ctggttttag ggaattgcac tcaaaagcaa 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 3480

3421 ggtgactcgc agtctggggc atcttttcta gtttcctaga agaactctag gaaaaaaaga 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 3540

3481 cgcgcattag acgacgaacg tttgtttttt tggtggcgat ggtcgccacc aaacaaacgg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 3600

3541 cctagttctc gatggttgag aaaaaggctt ccattgaccg aagtcgtctc gcgtctatgg 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 3660

3601 tttatgacaa gaagatcaca tcggcatcaa tccggtggtg aagttcttga gacatcgtgg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 3720

3661 cggatgtatg gagcgagacg attaggacaa tggtcaccga cgacggtcac cgctattcag 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 3780

3721 cacagaatgg cccaacctga gttctgctat caatggccta ttccgcgtcg ccagcccgac 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 3840

3781 ttgcccccca agcacgtgtg tcgggtcgaa cctcgcttgc tggatgtggc ttgactctat 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 3900

3841 ggatgtcgca ctcgatactc tttcgcggtg cgaagggctt ccctctttcc gcctgtccat 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 3960

3901 aggccattcg ccgtcccagc cttgtcctct cgcgtgctcc ctcgaaggtc cccctttgcg 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 4020

3961 gaccatagaa atatcaggac agcccaaagc ggtggagact gaactcgcag ctaaaaacac 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 4080

4021 tacgagcagt ccccccgcct cggatacctt tttgcggtcg ttgcgccgga aaaatgccaa 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cctggccttt tgctggcctt ttgctcacat ggctcgac.. .......... .......... 4118

4081 ggaccggaaa acgaccggaa aacgagtgta ccgagctg.. .......... .......... 4118

.......... .......... .......... .......... .......... ..........