KK%LOCUS pRS410 5270 bp DNA SYN 26-Aug-2007 DEFINITION pRS410 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5270 /organism="pRS410" /mol_type="other DNA" misc_feature complement(29..51) /label="pGEX_3_primer" gene complement(452..1231) /label="KanR2(variant)" /gene="KanR2(variant)" CDS complement(452..1261) /label="ORF frame 2" /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*" rep_origin complement(1854..2160) /label="f1_origin" misc_feature complement(2193..2336) /label="lacZ_a" misc_feature 2307..2329 /label="M13_pUC_fwd_primer" misc_feature 2322..2338 /label="M13_forward20_primer" promoter 2348..2366 /label="T7_promoter" misc_feature 2391..2407 /label="pBluescriptKS_primer" misc_feature complement(2442..2458) /label="pBluescriptSK_primer" promoter complement(2494..2513) /label="T3_promoter" misc_feature complement(2530..2548) /label="M13_reverse_primer" misc_feature complement(2547..2569) /label="M13_pUC_rev_primer" promoter complement(2583..2612) /label="lac_promoter" rep_origin complement(2921..3540) /label="pBR322_origin" CDS complement(3695..4555) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(3695..4555) /label="Ampicillin" /gene="Ampicillin" promoter complement(4597..4625) /label="AmpR_promoter" misc_feature 4687..5202 /label="CEN6_ARS4" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataatca tcgatgaatt cgagctcgtt ttcgacactg gatggcggcg ttagtatcga 241 atcgacagca gtatagcgac cagcattcac atacgattga cgcatgatat tactttctgc 301 gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata aatcacgcta 361 acatttgatt aaaatagaac aactacaata taaaaaaact atacaaatga caagttcttg 421 aaaacaagaa tctttttatt gtcagtactg attagaaaaa ctcatcgagc atcaaatgaa 481 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta 541 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg 601 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt 661 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat 721 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg 781 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc 841 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg 901 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttgc 961 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg 1021 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat 1081 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca 1141 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata 1201 aatcagcatc catgttggaa tttaatcgcg gcctcgaaac gtgagtcttt tccttaccca 1261 tggttgttta tgttcggatg tgatgtgaga actgtatcct agcaagattt taaaaggaag 1321 tatatgaaag aagaacctca gtggcaaatc ctaacctttt atatttctct acaggggcgc 1381 ggcgtgggga caattcaacg cgtctgtgag gggagcgttt ccctgctcgc aggtctgcag 1441 cgaggagccg taatttttgc ttcgcgccgt gcggccatca aaatgtatgg atgcaaatga 1501 ttatacatgg ggatgtatgg gctaaatgta cgggcgacag tcacatcatg cccctgagct 1561 gcgcacgtca agactgtcaa ggagggtatt ctgggcctcc atgtcgctgg ccgggtgacc 1621 cggcggggac gaggcaagct aaacagatct ggcgcgcctt aattaaccct atgcggtgtg 1681 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa acgttaatat 1741 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 1801 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 1861 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 1921 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 1981 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 2041 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 2101 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 2161 gccgctacag ggcgcgtcgc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 2221 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 2281 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 2341 gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga 2401 cggtatcgat aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagc 2461 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct 2521 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 2581 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 2641 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 2701 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 2761 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 2821 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 2881 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 2941 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 3001 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 3061 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 3121 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 3181 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 3241 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 3301 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 3361 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 3421 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 3481 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 3541 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 3601 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 3661 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 3721 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 3781 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 3841 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 3901 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 3961 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 4021 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 4081 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 4141 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 4201 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 4261 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 4321 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 4381 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 4441 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 4501 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 4561 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 4621 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 4681 cacctgGGTC CTTTTCATCA CGTGCTATAA AAATAATTAT AATTTAAATT TTTTAATATA 4741 AATATATAAA TTAAAAATAG AAAGTAAAAA AAGAAATTAA AGAAAAAATA GTTTTTGTTT 4801 TCCGAAGATG TAAAAGACTC TAGGGGGATC GCCAACAAAT ACTACCTTTT ATCTTGCTCT 4861 TCCTGCTCTC AGGTATTAAT GCCGAATTGT TTCATCTTGT CTGTGTAGAA GACCACACAC 4921 GAAAATCCTG TGATTTTACA TTTTACTTAT CGTTAATCGA ATGTATATCT ATTTAATCTG 4981 CTTTTCTTGT CTAATAAATA TATATGTAAA GTACGCTTTT TGTTGAAATT TTTTAAACCT 5041 TTGTTTATTT TTTTTTCTTC ATTCCGTAAC TCTTCTACCT TCTTTATTTA CTTTCTAAAA 5101 TCCAAATACA AAACATAAAA ATAAATAAAC ACAGAGTAAA TTCCCAAATT ATTCCATCAT 5161 TAAAAGATAC GAGGCGCGTG TAAGTTACAG GCAAGCGATC CGTCtctaag aaaccattat 5221 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc //pRS410 "Times New RomanKC3 pGEX_3_pri"Times New RomanKCKanR2(variant)"Times New Roman(KCɆORF2"Times New RomanK>p f1_origin"Times New RomanKC lacZ_a"Times New RomanKC   M13_pUC_f"Times New RomanKCɆ " M13_f20"Times New Roman)K, > T7"Times New RomanKCW g  pBlueKS_pri"Times New RomanKC  pBlueSK_pri"Times New RomanKCɆ T3"Times New RomanK M13_r"Times New RomanKC  M13_pUC_r"Times New RomanKC 4 lac"Times New RomanKCɆi  pBR322_origin"Times New RomanKoORF2"Times New Roman(KCo Ampicillin"Times New RomanKCAmpR_pro"Times New RomanKCɆOR CEN6_ARS4"Times New Roman KNruI"Times New Roman KNcoI"Times New Roman  KBglII"Times New Romanmknqjx K͑KpnI(2379), ApaI(2385), XhoI(2390), SalI(2396), EcoRV(2419), SmaI(2437), BamHI(2441), SpeI(2447), XbaI(2453), NotI(2460), EagI(2460), SacII(2472)"Times New Roman NZ/ [ KPmlI"Times New Roman];z4q-iZ-i Ktest"Times New Roman~ g