Sequence of pRS418

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Sequence of pRS418

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Sequence of pRS418 : 5043 BP; 1284 A; 1239 C; 1278 G; 1242 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60

1 agcgcgcaaa gccactactg ccacttttgg agactgtgta cgtcgagggc ctctgccagt 60

.......... .......... ........ pGEX_3_primer ........ ..........

.......... .......... .......... .......... .......... ..........

61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120

61 gtcgaacaga cattcgccta cggccctcgt ctgttcgggc agtcccgcgc agtcgcccac 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180

121 aaccgcccac agccccgacc gaattgatac gccgtagtct cgtctaacat gactctcacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 accataatca tcgatgaatt cgagctcgtt ttcgacactg gatggcggcg ttagtatcga 240

181 tggtattagt agctacttaa gctcgagcaa aagctgtgac ctaccgccgc aatcatagct 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 atcgacagca gtatagcgac cagcattcac atacgattga cgcatgatat tactttctgc 300

241 tagctgtcgt catatcgctg gtcgtaagtg tatgctaact gcgtactata atgaaagacg 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata aatcacgcta 360

301 cgtgaattga agcgtagacc cgtctactac agctccgctt ttttttatat ttagtgcgat 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 acatttgatt aaaatagaac aactacaata taaaaaaact atacaaatga caagttcttg 420

361 tgtaaactaa ttttatcttg ttgatgttat atttttttga tatgtttact gttcaagaac 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aaaacaagaa tctttttatt gtcagtactg attagaaaaa cctaggggca gggcatgctc 480

421 ttttgttctt agaaaaataa cagtcatgac taatcttttt ggatccccgt cccgtacgag 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atgtagagcg cctgccgctc gccgtccgag gcggtgccgt cgtacagggc ggtgtccagg 540

481 tacatctcgc ggacggcgag cggcaggctc cgccacggca gcatgtcccg ccacaggtcc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccgcagaggg tgaaccccat ccgccggtac gcgtggatcg ccggtgcgtt gacgttggtg 600

541 ggcgtctccc acttggggta ggcggccatg cgcacctagc ggccacgcaa ctgcaaccac 600

.......... .......... .......... .......... .......... ..........

.......... .......... NarI(622) .......... NruI(649) ..........

601 acctccagcc agaggtgccc ggcgccccgc tcgccggcga actccgtcgc gagccccatc 660

601 tggaggtcgg tctccacggg ccgcggggcg agcggccgct tgaggcagcg ctcggggtag 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 aacgcgcgcc cgaccccgtg cccccggtgc tccggggcga cctcgatgtc ctcgacggtc 720

661 ttgcgcgcgg gctggggcac gggggccacg aggccccgct ggagctacag gagctgccag 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 agccggcggt tccacgccga gtacgagatg accacgaagc ccgccaggtc gccgtcgtcc 780

721 tcggccgcca aggtgcggct catgctctac tggtgcttcg ggcggtccag cggcagcagg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ccgtacgcga cgaacgtccg ggagtccggg tcgccgtcct ccccgtcgtc cgattcgtcg 840

781 ggcatgcgct gcttgcaggc cctcaggccc agcggcagga ggggcagcag gctaagcagc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tccgattcgt cgtcggggaa caccttggtc aggggcgggt ccaccggcac ctcccgcagg 900

841 aggctaagca gcagcccctt gtggaaccag tccccgccca ggtggccgtg gagggcgtcc 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gtgaagccgt ccccggtggc ggtgacgcgg aagacggtgt cggtggtgaa ggacccatcc 960

901 cacttcggca ggggccaccg ccactgcgcc ttctgccaca gccaccactt cctgggtagg 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... AgeI(1004) ..........

961 agtgcctcga tggcctcggc gtcccccggg acactggtgc ggtaccggta agccgtgtcg 1020

961 tcacggagct accggagccg cagggggccc tgtgaccacg ccatggccat tcggcacagc 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcaagagtgg tcatggttgt ttatgttcgg atgtgatgtg agaactgtat cctagcaaga 1080

1021 agttctcacc agtaccaaca aatacaagcc tacactacac tcttgacata ggatcgttct 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ttttaaaagg aagtatatga aagaagaacc tcagtggcaa atcctaacct tttatatttc 1140

1081 aaaattttcc ttcatatact ttcttcttgg agtcaccgtt taggattgga aaatataaag 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tctacagggg cgcggcgtgg ggacaattca acgcgtctgt gaggggagcg tttccctgct 1200

1141 agatgtcccc gcgccgcacc cctgttaagt tgcgcagaca ctcccctcgc aaagggacga 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cgcaggtctg cagcgaggag ccgtaatttt tgcttcgcgc cgtgcggcca tcaaaatgta 1260

1201 gcgtccagac gtcgctcctc ggcattaaaa acgaagcgcg gcacgccggt agttttacat 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tggatgcaaa tgattataca tggggatgta tgggctaaat gtacgggcga cagtcacatc 1320

1261 acctacgttt actaatatgt acccctacat acccgattta catgcccgct gtcagtgtag 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 atgcccctga gctgcgcacg tcaagactgt caaggagggt attctgggcc tccatgtcgc 1380

1321 tacggggact cgacgcgtgc agttctgaca gttcctccca taagacccgg aggtacagcg 1380

.......... .......... .......... .......... .......... ..........

.......... .......... ......... BglII(1418) ......... ..........

1381 tggccgggtg acccggcggg gacgaggcaa gctaaacaga tctggcgcgc cttaattaac 1440

1381 accggcccac tgggccgccc ctgctccgtt cgatttgtct agaccgcgcg gaattaattg 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg 1500

1441 ggatacgcca cactttatgg cgtgtctacg cattcctctt ttatggcgta gtcctttaac 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 1560

1501 atttgcaatt ataaaacaat tttaagcgca atttaaaaac aatttagtcg agtaaaaaat 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 1620

1561 tggttatccg gctttagccg ttttagggaa tatttagttt tcttatctgg ctctatccca 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 1680

1621 actcacaaca aggtcaaacc ttgttctcag gtgataattt cttgcacctg aggttgcagt 1680

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 1740

1681 ttcccgcttt ttggcagata gtcccgctac cgggtgatgc acttggtagt gggattagtt 1740

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 1800

1741 caaaaaaccc cagctccacg gcatttcgtg atttagcctt gggatttccc tcgggggcta 1800

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 1860

1801 aatctcgaac tgcccctttc ggccgcttgc accgctcttt ccttcccttc tttcgctttc 1860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 1920

1861 ctcgcccgcg atcccgcgac cgttcacatc gccagtgcga cgcgcattgg tggtgtgggc 1920

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccgcgcttaa tgcgccgcta cagggcgcgt cgcgccattc gccattcagg ctgcgcaact 1980

1921 ggcgcgaatt acgcggcgat gtcccgcgca gcgcggtaag cggtaagtcc gacgcgttga 1980

f1_origin .......... .......... .......... .......... lacZ_a

.......... .......... .......... .......... .......... ..........

1981 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 2040

1981 caacccttcc cgctagccac gcccggagaa gcgataatgc ggtcgaccgc tttcccccta 2040

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 2100

2041 cacgacgttc cgctaattca acccattgcg gtcccaaaag ggtcagtgct gcaacatttt 2100

.......... .......... lacZ_a ...... M13_pUC_fwd_primer ......

.......... .......... .......... .......... .......... ..........

2101 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt accgggcccc 2160

2101 gctgccggtc actcgcgcgc attatgctga gtgatatccc gcttaaccca tggcccgggg 2160

lacZ_a M13_forward20_primer T7_promoter .......... ..........

XhoI SalI9) HindIII(2184) EcoRV(2192) . BamHI(2214)

2161 ccctcgaggt cgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca 2220

2161 gggagctcca gctgccatag ctattcgaac tatagcttaa ggacgtcggg ccccctaggt 2220

..... pBluescriptKS_primer ..... .......... .......... ..........

XbaI(2226) NotI(2233),EagI(2233) SacII(2245) ...... ..........

2221 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg 2280

2221 gatcaagatc tcgccggcgg tggcgccacc tcgaggtcga aaacaaggga aatcactccc 2280

pBluescriptSK_primer .......... .......... ......... T3_promoter

.......... .......... .......... .......... .......... ..........

2281 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 2340

2281 aattaacgcg cgaaccgcat tagtaccagt atcgacaaag gacacacttt aacaataggc 2340

.......... .......... .. M13_reverse_primer M13_pUC_rev_primer ..

.......... .......... .......... .......... .......... ..........

2341 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 2400

2341 gagtgttaag gtgtgttgta tgctcggcct tcgtatttca catttcggac cccacggatt 2400

.......... ......... lac_promoter ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 2460

2401 actcactcga ttgagtgtaa ttaacgcaac gcgagtgacg ggcgaaaggt cagccctttg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 2520

2461 gacagcacgg tcgacgtaat tacttagccg gttgcgcgcc cctctccgcc aaacgcataa 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 2580

2521 cccgcgagaa ggcgaaggag cgagtgactg agcgacgcga gccagcaagc cgacgccgct 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 2640

2581 cgccatagtc gagtgagttt ccgccattat gccaataggt gtcttagtcc cctattgcgt 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 2700

2641 cctttcttgt acactcgttt tccggtcgtt ttccggtcct tggcattttt ccggcgcaac 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 2760

2701 gaccgcaaaa aggtatccga ggcgggggga ctgctcgtag tgtttttagc tgcgagttca 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 2820

2761 gtctccaccg ctttgggctg tcctgatatt tctatggtcc gcaaaggggg accttcgagg 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 2880

2821 gagcacgcga gaggacaagg ctgggacggc gaatggccta tggacaggcg gaaagaggga 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 2940

2881 agcccttcgc accgcgaaag agtatcgagt gcgacatcca tagagtcaag ccacatccag 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 3000

2941 caagcgaggt tcgacccgac acacgtgctt ggggggcaag tcgggctggc gacgcggaat 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 3060

3001 aggccattga tagcagaact caggttgggc cattctgtgc tgaatagcgg tgaccgtcgt 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 3120

3061 cggtgaccat tgtcctaatc gtctcgctcc atacatccgc cacgatgtct caagaacttc 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 3180

3121 accaccggat tgatgccgat gtgatcttcc tgtcataaac catagacgcg agacgacttc 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 3240

3181 ggtcaatgga agcctttttc tcaaccatcg agaactaggc cgtttgtttg gtggcgacca 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 3300

3241 tcgccaccaa aaaaacaaac gttcgtcgtc taatgcgcgt ctttttttcc tagagttctt 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 3360

3301 ctaggaaact agaaaagatg ccccagactg cgagtcacct tgcttttgag tgcaattccc 3360

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 3420

3361 taaaaccagt actctaatag tttttcctag aagtggatct aggaaaattt aatttttact 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 3480

3421 tcaaaattta gttagatttc atatatactc atttgaacca gactgtcaat ggttacgaat 3480

.......... .......... .......... .......... .... Orf frame 3 Ampicillin

.......... .......... .......... .......... .......... ..........

3481 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 3540

3481 tagtcactcc gtggatagag tcgctagaca gataaagcaa gtaggtatca acggactgag 3540

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 3600

3541 gggcagcaca tctattgatg ctatgccctc ccgaatggta gaccggggtc acgacgttac 3600

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 3660

3601 tatggcgctc tgggtgcgag tggccgaggt ctaaatagtc gttatttggt cggtcggcct 3660

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 3720

3661 tcccggctcg cgtcttcacc aggacgttga aataggcgga ggtaggtcag ataattaaca 3720

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 3780

3721 acggcccttc gatctcattc atcaagcggt caattatcaa acgcgttgca acaacggtaa 3780

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 3840

3781 cgatgtccgt agcaccacag tgcgagcagc aaaccatacc gaagtaagtc gaggccaagg 3840

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 3900

3841 gttgctagtt ccgctcaatg tactaggggg tacaacacgt tttttcgcca atcgaggaag 3900

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 3960

3901 ccaggaggct agcaacagtc ttcattcaac cggcgtcaca atagtgagta ccaataccgt 3960

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 4020

3961 cgtgacgtat taagagaatg acagtacggt aggcattcta cgaaaagaca ctgaccactc 4020

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 4080

4021 atgagttggt tcagtaagac tcttatcaca tacgccgctg gctcaacgag aacgggccgc 4080

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 4140

4081 agttatgccc tattatggcg cggtgtatcg tcttgaaatt ttcacgagta gtaacctttt 4140

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 4200

4141 gcaagaagcc ccgcttttga gagttcctag aatggcgaca actctaggtc aagctacatt 4200

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 4260

4201 gggtgagcac gtgggttgac tagaagtcgt agaaaatgaa agtggtcgca aagacccact 4260

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 4320

4261 cgtttttgtc cttccgtttt acggcgtttt ttcccttatt cccgctgtgc ctttacaact 4320

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 4380

4321 tatgagtatg agaaggaaaa agttataata acttcgtaaa tagtcccaat aacagagtac 4380

.......... .......... .......... .......... ........ AmpR_promoter

.......... .......... .......... .......... .......... ..........

4381 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 4440

4381 tcgcctatgt ataaacttac ataaatcttt ttatttgttt atccccaagg cgcgtgtaaa 4440

AmpR_promoter ...... .......... .......... .......... ..........

.......... .......... .......... PmlI(4474) .......... ..........

4441 ccccgaaaag tgccacctgg gtccttttca tcacgtgcta taaaaataat tataatttaa 4500

4441 ggggcttttc acggtggacc caggaaaagt agtgcacgat atttttatta atattaaatt 4500

.......... .......... .......... CEN6_ARS4 .......... ..........

.......... .......... .......... .......... .......... ..........

4501 attttttaat ataaatatat aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa 4560

4501 taaaaaatta tatttatata tttaattttt atctttcatt tttttcttta atttcttttt 4560

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 atagtttttg ttttccgaag atgtaaaaga ctctaggggg atcgccaaca aatactacct 4620

4561 tatcaaaaac aaaaggcttc tacattttct gagatccccc tagcggttgt ttatgatgga 4620

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tttatcttgc tcttcctgct ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta 4680

4621 aaatagaacg agaaggacga gagtccataa ttacggctta acaaagtaga acagacacat 4680

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gaagaccaca cacgaaaatc ctgtgatttt acattttact tatcgttaat cgaatgtata 4740

4681 cttctggtgt gtgcttttag gacactaaaa tgtaaaatga atagcaatta gcttacatat 4740

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tctatttaat ctgcttttct tgtctaataa atatatatgt aaagtacgct ttttgttgaa 4800

4741 agataaatta gacgaaaaga acagattatt tatatataca tttcatgcga aaaacaactt 4800

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 attttttaaa cctttgttta tttttttttc ttcattccgt aactcttcta ccttctttat 4860

4801 taaaaaattt ggaaacaaat aaaaaaaaag aagtaaggca ttgagaagat ggaagaaata 4860

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttactttcta aaatccaaat acaaaacata aaaataaata aacacagagt aaattcccaa 4920

4861 aatgaaagat tttaggttta tgttttgtat ttttatttat ttgtgtctca tttaagggtt 4920

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 attattccat cattaaaaga tacgaggcgc gtgtaagtta caggcaagcg atccgtctct 4980

4921 taataaggta gtaattttct atgctccgcg cacattcaat gtccgttcgc taggcagaga 4980

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 5040

4981 ttctttggta ataatagtac tgtaattgga tatttttatc cgcatagtgc tccgggaaag 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gtc....... .......... .......... .......... .......... .......... 5043

5041 cag....... .......... .......... .......... .......... .......... 5043

.......... .......... .......... .......... .......... ..........