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    Plasmid map of pRS420

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    View the sequence with features on line
    pRS420
    Gene/insert name: none
    Insert size (bp): Unknown
    Vector backbone: pRS420 (Search Vector Database)
    Type of vector: Yeast expression
    Backbone size (bp): 6098
    5' Sequencing primer: T7 (List of Sequencing Primers)
    3' Sequencing primer: M13R
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Selectable markers: Neomycin
    If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: pRS400 from Jef Boeke.
    Plasmid Provided In: DH5a
    Principal Investigator: Fred Cross
    Comments: pRS400 + pRS425 --> pRS420. Insert is KanMX (G418 resistance) from AatII-cut pRS400. Backbone is ApaLI-cut pRS425 (2 micron plasmid).



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