Sequence of pRS420

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Sequence of pRS420

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Sequence of pRS420 : 6098 BP; 1651 A; 1398 C; 1438 G; 1611 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60

1 agcgcgcaaa gccactactg ccacttttgg agactgtgta cgtcgagggc ctctgccagt 60

.......... .......... .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120

61 gtcgaacaga cattcgccta cggccctcgt ctgttcgggc agtcccgcgc agtcgcccac 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180

121 aaccgcccac agccccgacc gaattgatac gccgtagtct cgtctaacat gactctcacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 accataatca tcgatgaatt cgagctcgtt ttcgacactg gatggcggcg ttagtatcga 240

181 tggtattagt agctacttaa gctcgagcaa aagctgtgac ctaccgccgc aatcatagct 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 atcgacagca gtatagcgac cagcattcac atacgattga cgcatgatat tactttctgc 300

241 tagctgtcgt catatcgctg gtcgtaagtg tatgctaact gcgtactata atgaaagacg 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata aatcacgcta 360

301 cgtgaattga agcgtagacc cgtctactac agctccgctt ttttttatat ttagtgcgat 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 acatttgatt aaaatagaac aactacaata taaaaaaact atacaaatga caagttcttg 420

361 tgtaaactaa ttttatcttg ttgatgttat atttttttga tatgtttact gttcaagaac 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aaaacaagaa tctttttatt gtcagtactg attagaaaaa ctcatcgagc atcaaatgaa 480

421 ttttgttctt agaaaaataa cagtcatgac taatcttttt gagtagctcg tagtttactt 480

.......... .......... .......... ..... KanR2(variant) ORF2 .....

.......... .......... .......... .......... .......... ..........

481 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta 540

481 tgacgttaaa taagtatagt cctaatagtt atggtataaa aactttttcg gcaaagacat 540

.......... ..... KanR2(variant) ORF2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg 600

541 tacttcctct tttgagtggc tccgtcaagg tatcctaccg ttctaggacc atagccagac 600

.......... ..... KanR2(variant) ORF2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

601 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt 660

601 gctaaggctg agcaggttgt agttatgttg gataattaaa ggggagcagt ttttattcca 660

.......... ..... KanR2(variant) ORF2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat 720

661 atagttcact ctttagtggt actcactgct gacttaggcc actcttaccg ttttcgaata 720

.......... ..... KanR2(variant) ORF2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg 780

721 cgtaaagaaa ggtctgaaca agttgtccgg tcggtaatgc gagcagtagt tttagtgagc 780

.......... ..... KanR2(variant) ORF2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

781 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc 840

781 gtagttggtt tggcaataag taagcactaa cgcggactcg ctctgcttta tgcgctagcg 840

.......... ..... KanR2(variant) ORF2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg 900

841 acaattttcc tgttaatgtt tgtccttagc ttacgttggc cgcgtccttg tgacggtcgc 900

.......... ..... KanR2(variant) ORF2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

901 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttgc 960

901 gtagttgtta taaaagtgga cttagtccta taagaagatt atggacctta cgacaaaacg 960

.......... ..... KanR2(variant) ORF2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg 1020

961 gcccctagcg tcaccactca ttggtacgta gtagtcctca tgcctatttt acgaactacc 1020

.......... ..... KanR2(variant) ORF2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat 1080

1021 agccttctcc gtatttaagg cagtcggtca aatcagactg gtagagtaga cattgtagta 1080

.......... ..... KanR2(variant) ORF2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca 1140

1081 accgttgcga tggaaacggt acaaagtctt tgttgagacc gcgtagcccg aagggtatgt 1140

.......... ..... KanR2(variant) ORF2 ..... .......... ..........

.......... .......... .......... NruI(1178) .......... ..........

1141 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata 1200

1141 tagctatcta acagcgtgga ctaacgggct gtaatagcgc tcgggtaaat atgggtatat 1200

.......... ..... KanR2(variant) ORF2 ..... .......... ..........

.......... .......... .......... .......... .......... NcoI(1258)

1201 aatcagcatc catgttggaa tttaatcgcg gcctcgaaac gtgagtcttt tccttaccca 1260

1201 ttagtcgtag gtacaacctt aaattagcgc cggagctttg cactcagaaa aggaatgggt 1260

........ KanR2(variant) ORF2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tggttgttta tgttcggatg tgatgtgaga actgtatcct agcaagattt taaaaggaag 1320

1261 accaacaaat acaagcctac actacactct tgacatagga tcgttctaaa attttccttc 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tatatgaaag aagaacctca gtggcaaatc ctaacctttt atatttctct acaggggcgc 1380

1321 atatactttc ttcttggagt caccgtttag gattggaaaa tataaagaga tgtccccgcg 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ggcgtgggga caattcaacg cgtctgtgag gggagcgttt ccctgctcgc aggtctgcag 1440

1381 ccgcacccct gttaagttgc gcagacactc ccctcgcaaa gggacgagcg tccagacgtc 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cgaggagccg taatttttgc ttcgcgccgt gcggccatca aaatgtatgg atgcaaatga 1500

1441 gctcctcggc attaaaaacg aagcgcggca cgccggtagt tttacatacc tacgtttact 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ttatacatgg ggatgtatgg gctaaatgta cgggcgacag tcacatcatg cccctgagct 1560

1501 aatatgtacc cctacatacc cgatttacat gcccgctgtc agtgtagtac ggggactcga 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcgcacgtca agactgtcaa ggagggtatt ctgggcctcc atgtcgctgg ccgggtgacc 1620

1561 cgcgtgcagt tctgacagtt cctcccataa gacccggagg tacagcgacc ggcccactgg 1620

.......... .......... .......... .......... .......... ..........

.......... ......... BglII(1645) ......... .......... ..........

1621 cggcggggac gaggcaagct aaacagatct ggcgcgcctt aattaaccct atgcggtgtg 1680

1621 gccgcccctg ctccgttcga tttgtctaga ccgcgcggaa ttaattggga tacgccacac 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa acgttaatat 1740

1681 tttatggcgt gtctacgcat tcctctttta tggcgtagtc ctttaacatt tgcaattata 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 1800

1741 aaacaatttt aagcgcaatt taaaaacaat ttagtcgagt aaaaaattgg ttatccggct 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 1860

1801 ttagccgttt tagggaatat ttagttttct tatctggctc tatcccaact cacaacaagg 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 1920

1861 tcaaaccttg ttctcaggtg ataatttctt gcacctgagg ttgcagtttc ccgctttttg 1920

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 1980

1921 gcagatagtc ccgctaccgg gtgatgcact tggtagtggg attagttcaa aaaaccccag 1980

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 2040

1981 ctccacggca tttcgtgatt tagccttggg atttccctcg ggggctaaat ctcgaactgc 2040

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 2100

2041 ccctttcggc cgcttgcacc gctctttcct tcccttcttt cgctttcctc gcccgcgatc 2100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 2160

2101 ccgcgaccgt tcacatcgcc agtgcgacgc gcattggtgg tgtgggcggc gcgaattacg 2160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gccgctacag ggcgcgtcgc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 2220

2161 cggcgatgtc ccgcgcagcg cggtaagcgg taagtccgac gcgttgacaa cccttcccgc 2220

.......... .......... .......... .......... lacZ_a ..........

.......... .......... .......... .......... .......... ..........

2221 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 2280

2221 tagccacgcc cggagaagcg ataatgcggt cgaccgcttt ccccctacac gacgttccgc 2280

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 2340

2281 taattcaacc cattgcggtc ccaaaagggt cagtgctgca acattttgct gccggtcact 2340

.......... .......... lacZ_a M13_pUC_f M13_f20 ..........

.......... .......... .... KpnI(2379) ApaI(2385),XhoI(2390) ....

2341 gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga 2400

2341 cgcgcgcatt atgctgagtg atatcccgct taacccatgg cccggggggg agctccagct 2400

.......... T7 .......... .......... ......... pBlueKS_pri

......... EcoRV(2419) ....... SmaI(2437),BamHI(2441) SpeI(2447)

2401 cggtatcgat aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagc 2460

2401 gccatagcta ttcgaactat agcttaagga cgtcgggccc cctaggtgat caagatctcg 2460

.......... .......... .......... ......... pBlueSK_pri .........

......... SacII(2472) ......... .......... .......... ..........

2461 ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct 2520

2461 ccggcggtgg cgccacctcg aggtcgaaaa caagggaaat cactcccaat taacgcgcga 2520

.......... .......... .......... .......... T3 ..........

.......... .......... .......... .......... .......... ..........

2521 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 2580

2521 accgcattag taccagtatc gacaaaggac acactttaac aataggcgag tgttaaggtg 2580

.......... M13_r .......... M13_pUC_r .......... ..........

.......... .......... .......... .......... .......... ..........

2581 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 2640

2581 tgttgtatgc tcggccttcg tatttcacat ttcggacccc acggattact cactcgattg 2640

.......... lac .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 2700

2641 agtgtaatta acgcaacgcg agtgacgggc gaaaggtcag ccctttggac agcacggtcg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 2760

2701 acgtaattac ttagccggtt gcgcgcccct ctccgccaaa cgcataaccc gcgagaaggc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 2820

2761 gaaggagcga gtgactgagc gacgcgagcc agcaagccga cgccgctcgc catagtcgag 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 2880

2821 tgagtttccg ccattatgcc aataggtgtc ttagtcccct attgcgtcct ttcttgtaca 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 2940

2881 ctcgttttcc ggtcgttttc cggtccttgg catttttccg gcgcaacgac cgcaaaaagg 2940

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2941 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 3000

2941 tatccgaggc ggggggactg ctcgtagtgt ttttagctgc gagttcagtc tccaccgctt 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 3060

3001 tgggctgtcc tgatatttct atggtccgca aagggggacc ttcgagggag cacgcgagag 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 3120

3061 gacaaggctg ggacggcgaa tggcctatgg acaggcggaa agagggaagc ccttcgcacc 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 3180

3121 gcgaaagagt atcgagtgcg acatccatag agtcaagcca catccagcaa gcgaggttcg 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 3240

3181 acccgacaca cgtgcttggg gggcaagtcg ggctggcgac gcggaatagg ccattgatag 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 3300

3241 cagaactcag gttgggccat tctgtgctga atagcggtga ccgtcgtcgg tgaccattgt 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 3360

3301 cctaatcgtc tcgctccata catccgccac gatgtctcaa gaacttcacc accggattga 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 3420

3361 tgccgatgtg atcttcctgt cataaaccat agacgcgaga cgacttcggt caatggaagc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 3480

3421 ctttttctca accatcgaga actaggccgt ttgtttggtg gcgaccatcg ccaccaaaaa 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 3540

3481 aacaaacgtt cgtcgtctaa tgcgcgtctt tttttcctag agttcttcta ggaaactaga 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 3600

3541 aaagatgccc cagactgcga gtcaccttgc ttttgagtgc aattccctaa aaccagtact 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 3660

3601 ctaatagttt ttcctagaag tggatctagg aaaatttaat ttttacttca aaatttagtt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 3720

3661 agatttcata tatactcatt tgaaccagac tgtcaatggt tacgaattag tcactccgtg 3720

.......... .......... .......... ....... ORF2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

3721 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 3780

3721 gatagagtcg ctagacagat aaagcaagta ggtatcaacg gactgagggg cagcacatct 3780

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 3840

3781 attgatgcta tgccctcccg aatggtagac cggggtcacg acgttactat ggcgctctgg 3840

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 3900

3841 gtgcgagtgg ccgaggtcta aatagtcgtt atttggtcgg tcggccttcc cggctcgcgt 3900

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 3960

3901 cttcaccagg acgttgaaat aggcggaggt aggtcagata attaacaacg gcccttcgat 3960

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 4020

3961 ctcattcatc aagcggtcaa ttatcaaacg cgttgcaaca acggtaacga tgtccgtagc 4020

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 4080

4021 accacagtgc gagcagcaaa ccataccgaa gtaagtcgag gccaagggtt gctagttccg 4080

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 4140

4081 ctcaatgtac tagggggtac aacacgtttt ttcgccaatc gaggaagcca ggaggctagc 4140

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 4200

4141 aacagtcttc attcaaccgg cgtcacaata gtgagtacca ataccgtcgt gacgtattaa 4200

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 4260

4201 gagaatgaca gtacggtagg cattctacga aaagacactg accactcatg agttggttca 4260

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 4320

4261 gtaagactct tatcacatac gccgctggct caacgagaac gggccgcagt tatgccctat 4320

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 4380

4321 tatggcgcgg tgtatcgtct tgaaattttc acgagtagta accttttgca agaagccccg 4380

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 4440

4381 cttttgagag ttcctagaat ggcgacaact ctaggtcaag ctacattggg tgagcacgtg 4440

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 4500

4441 ggttgactag aagtcgtaga aaatgaaagt ggtcgcaaag acccactcgt ttttgtcctt 4500

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 4560

4501 ccgttttacg gcgttttttc ccttattccc gctgtgcctt tacaacttat gagtatgaga 4560

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 4620

4561 aggaaaaagt tataataact tcgtaaatag tcccaataac agagtactcg cctatgtata 4620

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

4621 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 4680

4621 aacttacata aatcttttta tttgtttatc cccaaggcgc gtgtaaaggg gcttttcacg 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta 4740

4681 gtggacttgc ttcgtagaca cgaagtaaaa catcttgttt ttacgttgcg ctctcgcgat 4740

.......... .......... ....... 2micron2_origin ....... ..........

.......... .......... .......... .......... .......... ..........

4741 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg 4800

4741 taaaaagttt gtttcttaga ctcgacgtaa aaatgtcttg tctttacgtt gcgctttcgc 4800

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag 4860

4801 gataaaatgg ttgcttctta gacacgaagt aaaaacattt tgtttttacg ttgcgctctc 4860

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga 4920

4861 gcgattaaaa agtttgtttc ttagactcga cgtaaaaatg tcttgtcttt acgttgcgct 4920

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc 4980

4921 ctcgcgataa aatggttgtt tcttagatat gaagaaaaaa caagatgttt ttacgtaggg 4980

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct 5040

4981 ctctcgcgat aaaaagattg tttcgtagaa tctaatgaaa aaaagaggaa acacgcgaga 5040

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta 5100

5041 tattacgtca gagaactatt gaaaaacgtg acatccaggc aattccaatc ttcttccgat 5100

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga 5160

5101 gaaaccacag ataaaagaga aggtattttt ttcggactga ggtgaagggc gcaaatgact 5160

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat tatattctat 5220

5161 aatgatcgct tcgacgccca cgtaaaaaag ttctatttcc gtaggggcta atataagata 5220

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt 5280

5221 tggctacacc taacgcgtat gaaacacttg tctttcacta tcgcaactac taagaagtaa 5280

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg 5340

5281 ccagtctttt aatacttgcc aaagaagata aaacagagat atatgatgca tatcctttac 5340

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg 5400

5341 aaatgtaaaa gcataacaaa agctaagtga gatacttatc aagaatgatg ttaaaaaaac 5400

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt 5460

5401 agatttctca ttatgatctc tatttgtatt ttttacatct ccagctcaaa tctacgttca 5460

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca 5520

5461 agttcctcgc tttccaccta cccatccaat atatccctat atcgtgtctc tatatatcgt 5520

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt 5580

5521 ttctctatga aaactcgtta caaacacctt cgccataagc gttataaaat catcgagcaa 5580

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa 5640

5581 tgtcaggcca cgcaaaaacc aaaaaacttt cacgcagaag tctcgcgaaa accaaaagtt 5640

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaaa 5700

5641 ttcgcgagac ttcaaggata tgaaagatct cttatccttg aagccttatc cttgaagttt 5700

2micron2_origin .... .......... FRT .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact 5760

5701 cgcaaaggct tttgctcgcg aaggctttta cgttgcgctc gacgcgtgta tgtcgagtga 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc 5820

5761 caagtgcagc gtggatatag acgcacaacg gacatatata tatatgtact cttcttgccg 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg 5880

5821 tatcacgcac aaatacgaat ttacgcatga atatacgcag ataaatacat cctactttcc 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca 5940

5881 atcagatcat ggaggacact ataatagggt aaggtacgcc ccatagcata cgaaggaagt 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc 6000

5941 cgtgatggga aatcgacaag atatacgacg gtgaggagtt aacctaatca gagtaggaag 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 aatgctatca tttcctttga tattggatca tatctaagaa accattatta tcatgacatt 6060

6001 ttacgatagt aaaggaaact ataacctagt atagattctt tggtaataat agtactgtaa 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 aacctataaa aataggcgta tcacgaggcc ctttcgtc.. .......... .......... 6098

6061 ttggatattt ttatccgcat agtgctccgg gaaagcag.. .......... .......... 6098

.......... .......... .......... .......... .......... ..........