BB%LOCUS pRS428 5871 bp DNA SYN 24-Aug-2007 DEFINITION pRS428 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5871 /organism="pRS428" /mol_type="other DNA" misc_feature complement(29..51) /label="pGEX_3_primer" rep_origin complement(1627..1933) /label="f1_origin" misc_feature complement(1966..2109) /label="lacZ_a" misc_feature 2080..2102 /label="M13_pUC_fwd_primer" misc_feature 2095..2111 /label="M13_forward20_primer" promoter 2121..2139 /label="T7_promoter" misc_feature 2164..2180 /label="pBluescriptKS_primer" misc_feature complement(2215..2231) /label="pBluescriptSK_primer" promoter complement(2267..2286) /label="T3_promoter" misc_feature complement(2303..2321) /label="M13_reverse_primer" misc_feature complement(2320..2342) /label="M13_pUC_rev_primer" promoter complement(2356..2385) /label="lac_promoter" rep_origin complement(2694..3313) /label="pBR322_origin" CDS complement(3468..4328) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(3468..4328) /label="Ampicillin" /gene="Ampicillin" promoter complement(4370..4398) /label="AmpR_promoter" rep_origin complement(4460..5431) /label="2micron2_origin" misc_feature complement(5423..5470) /label="FRT" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataatca tcgatgaatt cgagctcgtt ttcgacactg gatggcggcg ttagtatcga 241 atcgacagca gtatagcgac cagcattcac atacgattga cgcatgatat tactttctgc 301 gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata aatcacgcta 361 acatttgatt aaaatagaac aactacaata taaaaaaact atacaaatga caagttcttg 421 aaaacaagaa tctttttatt gtcagtactg attagaaaaa cctaggggca gggcatgctc 481 atgtagagcg cctgccgctc gccgtccgag gcggtgccgt cgtacagggc ggtgtccagg 541 ccgcagaggg tgaaccccat ccgccggtac gcgtggatcg ccggtgcgtt gacgttggtg 601 acctccagcc agaggtgccc ggcgccccgc tcgccggcga actccgtcgc gagccccatc 661 aacgcgcgcc cgaccccgtg cccccggtgc tccggggcga cctcgatgtc ctcgacggtc 721 agccggcggt tccacgccga gtacgagatg accacgaagc ccgccaggtc gccgtcgtcc 781 ccgtacgcga cgaacgtccg ggagtccggg tcgccgtcct ccccgtcgtc cgattcgtcg 841 tccgattcgt cgtcggggaa caccttggtc aggggcgggt ccaccggcac ctcccgcagg 901 gtgaagccgt ccccggtggc ggtgacgcgg aagacggtgt cggtggtgaa ggacccatcc 961 agtgcctcga tggcctcggc gtcccccggg acactggtgc ggtaccggta agccgtgtcg 1021 tcaagagtgg tcatggttgt ttatgttcgg atgtgatgtg agaactgtat cctagcaaga 1081 ttttaaaagg aagtatatga aagaagaacc tcagtggcaa atcctaacct tttatatttc 1141 tctacagggg cgcggcgtgg ggacaattca acgcgtctgt gaggggagcg tttccctgct 1201 cgcaggtctg cagcgaggag ccgtaatttt tgcttcgcgc cgtgcggcca tcaaaatgta 1261 tggatgcaaa tgattataca tggggatgta tgggctaaat gtacgggcga cagtcacatc 1321 atgcccctga gctgcgcacg tcaagactgt caaggagggt attctgggcc tccatgtcgc 1381 tggccgggtg acccggcggg gacgaggcaa gctaaacaga tctggcgcgc cttaattaac 1441 cctatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg 1501 taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 1561 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 1621 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 1681 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 1741 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 1801 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 1861 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 1921 ccgcgcttaa tgcgccgcta cagggcgcgt cgcgccattc gccattcagg ctgcgcaact 1981 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 2041 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 2101 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt accgggcccc 2161 ccctcgaggt cgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca 2221 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg 2281 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 2341 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 2401 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 2461 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 2521 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 2581 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 2641 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 2701 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 2761 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 2821 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 2881 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 2941 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 3001 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 3061 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 3121 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 3181 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 3241 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 3301 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 3361 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 3421 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 3481 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 3541 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 3601 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 3661 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 3721 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 3781 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 3841 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 3901 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 3961 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 4021 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 4081 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 4141 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 4201 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 4261 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 4321 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 4381 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 4441 ccccgaaaag tgccacctgA ACGAAGCATC TGTGCTTCAT TTTGTAGAAC AAAAATGCAA 4501 CGCGAGAGCG CTAATTTTTC AAACAAAGAA TCTGAGCTGC ATTTTTACAG AACAGAAATG 4561 CAACGCGAAA GCGCTATTTT ACCAACGAAG AATCTGTGCT TCATTTTTGT AAAACAAAAA 4621 TGCAACGCGA GAGCGCTAAT TTTTCAAACA AAGAATCTGA GCTGCATTTT TACAGAACAG 4681 AAATGCAACG CGAGAGCGCT ATTTTACCAA CAAAGAATCT ATACTTCTTT TTTGTTCTAC 4741 AAAAATGCAT CCCGAGAGCG CTATTTTTCT AACAAAGCAT CTTAGATTAC TTTTTTTCTC 4801 CTTTGTGCGC TCTATAATGC AGTCTCTTGA TAACTTTTTG CACTGTAGGT CCGTTAAGGT 4861 TAGAAGAAGG CTACTTTGGT GTCTATTTTC TCTTCCATAA AAAAAGCCTG ACTCCACTTC 4921 CCGCGTTTAC TGATTACTAG CGAAGCTGCG GGTGCATTTT TTCAAGATAA AGGCATCCCC 4981 GATTATATTC TATACCGATG TGGATTGCGC ATACTTTGTG AACAGAAAGT GATAGCGTTG 5041 ATGATTCTTC ATTGGTCAGA AAATTATGAA CGGTTTCTTC TATTTTGTCT CTATATACTA 5101 CGTATAGGAA ATGTTTACAT TTTCGTATTG TTTTCGATTC ACTCTATGAA TAGTTCTTAC 5161 TACAATTTTT TTGTCTAAAG AGTAATACTA GAGATAAACA TAAAAAATGT AGAGGTCGAG 5221 TTTAGATGCA AGTTCAAGGA GCGAAAGGTG GATGGGTAGG TTATATAGGG ATATAGCACA 5281 GAGATATATA GCAAAGAGAT ACTTTTGAGC AATGTTTGTG GAAGCGGTAT TCGCAATATT 5341 TTAGTAGCTC GTTACAGTCC GGTGCGTTTT TGGTTTTTTG AAAGTGCGTC TTCAGAGCGC 5401 TTTTGGTTTT CAAAAGCGCT CTGAAGTTCC TATACTTTCT AGAGAATAGG AACTTCGGAA 5461 TAGGAACTTC AAAGCGTTTC CGAAAACGAG CGCTTCCGAA AATGCAACGC GAGCTGCGCA 5521 CATACAGCTC ACTGTTCACG TCGCACCTAT ATCTGCGTGT TGCCTGTATA TATATATACA 5581 TGAGAAGAAC GGCATAGTGC GTGTTTATGC TTAAATGCGT ACTTATATGC GTCTATTTAT 5641 GTAGGATGAA AGGTAGTCTA GTACCTCCTG TGATATTATC CCATTCCATG CGGGGTATCG 5701 TATGCTTCCT TCAGCACTAC CCTTTAGCTG TTCTATATGC TGCCACTCCT CAATTGGATT 5761 AGTCTCATCC TTCAATGCTA TCATTTCCTT TGATATTGGA TCATAtctaa gaaaccatta 5821 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt c //pRS428 "Times New RomanBC3 pGEX_3_primer"Times New RomanBC/FRT"Times New RomanBC[ f1_origin"Times New RomanBCɆ=lacZ_a"Times New RomanB 6 M13_pUC_f"Times New RomanBC/?M13_f20"Times New Roman)BCI[T7"Times New RomanBCɆtpBluesKS"Times New RomanBpBluesSK"Times New RomanBCT3"Times New RomanBC M13_r"Times New RomanBCɆ &  M13_pUC_r"Times New Roman)B4 Q lac"Times New RomanBC  pBR322_origin"Times New RomanBC  Orf frame 3"Times New Roman(BCɆ  Ampicillin"Times New RomanB. AmpR_promoter"Times New RomanBCl72micron2_origin"Times New Roman BNarI"Times New Romann>oQd]T]ET BNruI"Times New Roman AqWj`e`^l BAgeI"Times New Roman  v BBglII"Times New Roman  $%, B|ApaI(2158), XhoI(2163), SalI(2169), HindIII(2184), EcoRV(2192), BamHI(2214), SpeI(2220), NotI(2233), EagI(2233), SacII(2245)"Times New RomanTTEaa9 Btest"Times New RomanG&M