Sequence of pRSV-Rev

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Sequence of pRSV-Rev

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Sequence of pRSV-Rev : 4174 BP; 1041 A; 1031 C; 1056 G; 1046 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60

1 tcgcgggtta tgcgtttggc ggagaggggc gcgcaaccgg ctaagtaatt acgtcgaccg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120

61 tgctgtccaa agggctgacc tttcgcccgt cactcgcgtt gcgttaatta cactcaatcg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180

121 agtgagtaat ccgtggggtc cgaaatgtga aatacgaagg ccgagcatac aacacacctt 180

.......... .......... ......... lac_promoter ......... ..........

.......... .......... .......... .......... .......... ..........

181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaattcgatg 240

181 aacactcgcc tattgttaaa gtgtgtcctt tgtcgatact ggtactaatg cttaagctac 240

...... M13_pUC_rev_primer .. M13_reverse_primer ...... ..........

.......... .......... .......... .......... .......... ..........

241 tacgggccag atatacgcgt atctgagggg actaggtgtg tttaggcgaa aagcggggct 300

241 atgcccggtc tatatgcgca tagactcccc tgatccacac aaatccgctt ttcgccccga 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tcggttgtac gcggttagga gtcccctcag gattagtagt ttcgcttttg catagggagg 360

301 agccaacatg cgccaatcct caggggagtc ctaatcatca aagcgaaaac gtatccctcc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gggaaatgta gtcttatgca atacacttgt agtcttgcaa catggtaacg atgagttagc 420

361 ccctttacat cagaatacgt tatgtgaaca tcagaacgtt gtaccattgc tactcaatcg 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt 480

421 ttgtacggaa tgttcctctc tttttcgtgg cacgtacggc taaccacctt cattccacca 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 acgatcgtgc cttattagga aggcaacaga caggtctgac atggattgga cgaaccactg 540

481 tgctagcacg gaataatcct tccgttgtct gtccagactg tacctaacct gcttggtgac 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aattccgcat tgcagagata attgtattta agtgcctagc tcgatacaat aaacgccatt 600

541 ttaaggcgta acgtctctat taacataaat tcacggatcg agctatgtta tttgcggtaa 600

.......... .......... .......... ....... pREP_fwd_primer .......

.......... .......... .......... XhoI(634) SacI(641) ..........

601 tgaccattca ccacattggt gtgcacctcc aagctcgagc tcgtttagtg aaccgtcaga 660

601 actggtaagt ggtgtaacca cacgtggagg ttcgagctcg agcaaatcac ttggcagtct 660

.......... .......... .......... ......... LNCX_primer .........

.......... .......... .......... .......... .......... ..........

661 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca 720

661 agcggacctc tgcggtaggt gcgacaaaac tggaggtatc ttctgtggcc ctggctaggt 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gcctcccctc gaagctagtc gattaggcat ctcctatggc aggaagaagc ggagacagcg 780

721 cggaggggag cttcgatcag ctaatccgta gaggataccg tccttcttcg cctctgtcgc 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 acgaagacct cctcaaggca gtcagactca tcaagtttct ctatcaaagc aacccacctc 840

781 tgcttctgga ggagttccgt cagtctgagt agttcaaaga gatagtttcg ttgggtggag 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ccaatcccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac 900

841 ggttagggct cccctgggct gtccgggctt ccttatcttc ttcttccacc tctctctctg 900

.......... .......... .......... .......... .......... ..........

.......... .......... BamHI(928) .......... .......... ..........

901 agagacagat ccattcgatt agtgaacgga tccttagcac ttatctggga cgatctgcgg 960

901 tctctgtcta ggtaagctaa tcacttgcct aggaatcgtg aatagaccct gctagacgcc 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 agcctgtgcc tcttcagcta ccaccgcttg agagacttac tcttgattgt aacgaggatt 1020

961 tcggacacgg agaagtcgat ggtggcgaac tctctgaatg agaactaaca ttgctcctaa 1020

.......... .......... ......... HIV_Rev_NES ......... ..........

.......... .......... .......... .......... .......... ..........

1021 gtggaacttc tgggacgcag ggggtgggaa gccctcaaat attggtggaa tctcctacaa 1080

1021 caccttgaag accctgcgtc ccccaccctt cgggagttta taaccacctt agaggatgtt 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tattggagtc aggagctaaa gaatagtgct gttagcttgc tcaatgccac agctatagca 1140

1081 ataacctcag tcctcgattt cttatcacga caatcgaacg agttacggtg tcgatatcgt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtagctgagg ggacagatag ggttatagaa gtagtacaag aagcttggca ctggccgtcg 1200

1141 catcgactcc cctgtctatc ccaatatctt catcatgttc ttcgaaccgt gaccggcagc 1200

.......... .......... .......... .......... ..... M13_forward20_primer

.......... ......... BglII(1223) ......... ......... AflII(1252)

1201 ttttacatga tctgagcctg ggagatctct ggctaactag ggaacccact gcttaagcct 1260

1201 aaaatgtact agactcggac cctctagaga ccgattgatc ccttgggtga cgaattcgga 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 1320

1261 gttatttcga acggaactca cgaagttcat cacacacggg cagacaacac actgagacca 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aactagagat caggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 1380

1321 ttgatctcta gtccttttgg gaccgcaatg ggttgaatta gcggaacgtc gtgtaggggg 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 1440

1381 aaagcggtcg accgcattat cgcttctccg ggcgtggcta gcgggaaggg ttgtcaacgc 1440

.......... .......... .......... .......... .......... ..........

.......... NarI(1459) .......... .......... .......... ..........

1441 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat 1500

1441 gtcggactta ccgcttaccg cggactacgc cataaaagag gaatgcgtag acacgccata 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg 1560

1501 aagtgtggcg tatgcagttt cgttggtatc atgcgcggga catcgccgcg taattcgcgc 1560

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

1561 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 1620

1561 cgcccacacc accaatgcgc gtcgcactgg cgatgtgaac ggtcgcggga tcgcgggcga 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 1680

1621 ggaaagcgaa agaagggaag gaaagagcgg tgcaagcggc cgaaaggggc agttcgagat 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 1740

1681 ttagcccccg agggaaatcc caaggctaaa tcacgaaatg ccgtggagct ggggtttttt 1740

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 1800

1741 gaactaaacc cactaccaag tgcatcaccc ggtagcggga ctatctgcca aaaagcggga 1800

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 1860

1801 aactgcaacc tcaggtgcaa gaaattatca cctgagaaca aggtttgacc ttgttgtgag 1860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg 1920

1861 ttgggataga gcccgataag aaaactaaat attccctaaa acggctaaag ccggataacc 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt 1980

1921 aattttttac tcgactaaat tgtttttaaa ttgcgcttaa aattgtttta taattgcaaa 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc 2040

1981 tgttaaaata ccacgtgaga gtcatgttag acgagactac ggcgtatcaa ttcggtcggg 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct 2100

2041 gctgtgggcg gttgtgggcg actgcgcggg actgcccgaa cagacgaggg ccgtaggcga 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca 2160

2101 atgtctgttc gacactggca gaggccctcg acgtacacag tctccaaaag tggcagtagt 2160

.......... .......... ........ pGEX_3_primer ........ ..........

.......... .......... .......... .......... .......... ..........

2161 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg 2220

2161 ggctttgcgc gctctgcttt cccggagcac tatgcggata aaaatatcca attacagtac 2220

.......... .......... .......... .......... .......... ..........

.......... ......... AatII(2242) ......... .......... ..........

2221 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 2280

2221 tattattacc aaagaatctg cagtccaccg tgaaaagccc ctttacacgc gccttgggga 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 2340

2281 taaacaaata aaaagattta tgtaagttta tacataggcg agtactctgt tattgggact 2340

.......... .......... ........ AmpR_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

2341 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 2400

2341 atttacgaag ttattataac tttttccttc tcatactcat aagttgtaaa ggcacagcgg 2400

.......... .......... .......... .... Orf frame 1 Ampicillin ....

.......... .......... .......... .......... .......... ..........

2401 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 2460

2401 gaataaggga aaaaacgccg taaaacggaa ggacaaaaac gagtgggtct ttgcgaccac 2460

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 2520

2461 tttcattttc tacgacttct agtcaaccca cgtgctcacc caatgtagct tgacctagag 2520

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 2580

2521 ttgtcgccat tctaggaact ctcaaaagcg gggcttcttg caaaaggtta ctactcgtga 2580

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 2640

2581 aaatttcaag acgatacacc gcgccataat agggcataac tgcggcccgt tctcgttgag 2640

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 2700

2641 ccagcggcgt atgtgataag agtcttactg aaccaactca tgagtggtca gtgtcttttc 2700

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 2760

2701 gtagaatgcc taccgtactg tcattctctt aatacgtcac gacggtattg gtactcacta 2760

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 2820

2761 ttgtgacgcc ggttgaatga agactgttgc tagcctcctg gcttcctcga ttggcgaaaa 2820

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 2880

2821 aacgtgttgt accccctagt acattgagcg gaactagcaa cccttggcct cgacttactt 2880

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 2940

2881 cggtatggtt tgctgctcgc actgtggtgc tacggacatc gttaccgttg ttgcaacgcg 2940

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 3000

2941 tttgataatt gaccgcttga tgaatgagat cgaagggccg ttgttaatta tctgacctac 3000

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 3060

3001 ctccgcctat ttcaacgtcc tggtgaagac gcgagccggg aaggccgacc gaccaaataa 3060

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 3120

3061 cgactattta gacctcggcc actcgcaccc agagcgccat agtaacgtcg tgaccccggt 3120

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 3180

3121 ctaccattcg ggagggcata gcatcaatag atgtgctgcc cctcagtccg ttgataccta 3180

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 3240

3181 cttgctttat ctgtctagcg actctatcca cggagtgact aattcgtaac cattgacagt 3240

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 3300

3241 ctggttcaaa tgagtatata tgaaatctaa ctaaattttg aagtaaaaat taaattttcc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 3360

3301 tagatccact tctaggaaaa actattagag tactggtttt agggaattgc actcaaaagc 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 3420

3361 aaggtgactc gcagtctggg gcatcttttc tagtttccta gaagaactct aggaaaaaaa 3420

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3421 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 3480

3421 gacgcgcatt agacgacgaa cgtttgtttt tttggtggcg atggtcgcca ccaaacaaac 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 3540

3481 ggcctagttc tcgatggttg agaaaaaggc ttccattgac cgaagtcgtc tcgcgtctat 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 3600

3541 ggtttatgac aggaagatca catcggcatc aatccggtgg tgaagttctt gagacatcgt 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 3660

3601 ggcggatgta tggagcgaga cgattaggac aatggtcacc gacgacggtc accgctattc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 3720

3661 agcacagaat ggcccaacct gagttctgct atcaatggcc tattccgcgt cgccagcccg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 3780

3721 acttgccccc caagcacgtg tgtcgggtcg aacctcgctt gctggatgtg gcttgactct 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 3840

3781 atggatgtcg cactcgatac tctttcgcgg tgcgaagggc ttccctcttt ccgcctgtcc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 3900

3841 ataggccatt cgccgtccca gccttgtcct ctcgcgtgct ccctcgaagg tccccctttg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 3960

3901 cggaccatag aaatatcagg acagcccaaa gcggtggaga ctgaactcgc agctaaaaac 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 4020

3961 actacgagca gtccccccgc ctcggatacc tttttgcggt cgttgcgccg gaaaaatgcc 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 4080

4021 aaggaccgga aaacgaccgg aaaacgagtg tacaagaaag gacgcaatag gggactaaga 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 4140

4081 cacctattgg cataatggcg gaaactcact cgactatggc gagcggcgtc ggcttgctgg 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gagcgcagcg agtcagtgag cgaggaagcg gaag...... .......... .......... 4174

4141 ctcgcgtcgc tcagtcactc gctccttcgc cttc...... .......... .......... 4174

.......... .......... .......... .......... .......... ..........