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pRho-Cre
Gene/insert name: Rhodopsin promoter-Cre
Alternative names: Cre recombinase
Insert size (bp): 3393
Fusion proteins or tags: Myc
Terminal: C terminal on insert
Vector backbone: pCAGEN? (Search Vector Database)
Type of vector: Mammalian expression,Cre/Lox
Backbone size (bp): 6454
Cloning site 5': EcoRI
Site destroyed during cloning: No
Cloning site 3': NotI
Site destroyed during cloning: No
5' Sequencing primer: na (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: The bovine rhodopsin promoter was from -2174-lacZ (Zack et al., Neuron 6, 187-199 (1991)) obtained from Dr. D. Zack (Johns Hopkins University). The coding sequence of Cre was amplified by PCR using pxCANCre (Kanegae et al. NAR 23, 3816-3821 (1995)) obtained from Dr. Saito I (Univ. of Tokyo) as a template.
Plasmid Provided In: DH5a
Principal Investigator: Connie Cepko
Comments: The rhodopsin promoter-Cre is specific for photorecetor cells in the retina. Cre has a Myc-tag at its C-terminus.
Article: Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T et al. (Proc Natl Acad Sci U S A. 2007 Jan 5. ():. Pubmed)