Sequence of pRho-Cre

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Sequence of pRho-Cre

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Sequence of pRho-Cre : 6454 BP; 1449 A; 1787 C; 1657 G; 1561 T; 0 other;

SalI(1) .......... .......... .......... .......... ..........

1 gtcgacattg attattgact agccagcttc cacgttgtca gaagcgttag cgggtaacaa 60

1 cagctgtaac taataactga tcggtcgaag gtgcaacagt cttcgcaatc gcccattgtt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gcaaggccat gagtttgagg gtcgggggga cagcctggcc agggcagggc tgcgtgctct 120

61 cgttccggta ctcaaactcc cagcccccct gtcggaccgg tcccgtcccg acgcacgaga 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggtcttgacc cagggagtcc atcgtctgtc cctctgtctg gccaccaggg gcgtgttaga 180

121 ccagaactgg gtccctcagg tagcagacag ggagacagac cggtggtccc cgcacaatct 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 atcaaactct tcaccttgac ctctttagcg atggacctaa tcacagggcc aagcaccttg 240

181 tagtttgaga agtggaactg gagaaatcgc tacctggatt agtgtcccgg ttcgtggaac 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 agcagctgcg ccccacccac ccgccacacc tgcctgcccc ttgctccttc cccccagagc 300

241 tcgtcgacgc ggggtgggtg ggcggtgtgg acggacgggg aacgaggaag gggggtctcg 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 acttaccacc aggctcccca cacaagtcct gatgctgccc gctgcccggg acagcagggc 360

301 tgaatggtgg tccgaggggt gtgttcagga ctacgacggg cgacgggccc tgtcgtcccg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... PmlI(395) .......... ..........

361 tctggagccc aggtgggctg cagcgaaggg agcacgtgtc tgagcagaga ggtgtagaca 420

361 agacctcggg tccacccgac gtcgcttccc tcgtgcacag actcgtctct ccacatctgt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tcccaggtgg taagagggac agcacaaggg agcctcagcg atggaggtct cagacactgg 480

421 agggtccacc attctccctg tcgtgttccc tcggagtcgc tacctccaga gtctgtgacc 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gatgcttcct ggccaccctt gcgttcccca cctcggggcc tttgcactgg ccgctctctc 540

481 ctacgaagga ccggtgggaa cgcaaggggt ggagccccgg aaacgtgacc ggcgagagag 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... XhoI(578) .......... ..........

541 tccttggaca gctccacccc ttagtgctcc ttccatcctc gagttctcag agaggccttc 600

541 aggaacctgt cgaggtgggg aatcacgagg aaggtaggag ctcaagagtc tctccggaag 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ctgggcggct ccaggcaact gtgtgtcggg aggacgtcac catctgaaca gtccctgtgg 660

601 gacccgccga ggtccgttga cacacagccc tcctgcagtg gtagacttgt cagggacacc 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tcgtttgttt catggtttgt gggggcccct ccccgccccc ccagaagaag gacacgtcct 720

661 agcaaacaaa gtaccaaaca cccccgggga ggggcggggg ggtcttcttc ctgtgcagga 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 acaggcaggg gcttggtcct tcttgtccac tgtgtccccc agaactgagc gtcttgtgta 780

721 tgtccgtccc cgaaccagga agaacaggtg acacaggggg tcttgactcg cagaacacat 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gaacacagtc ggtgccagat aaaactcaca gctggactgt ggtcattgag gaccccgaag 840

781 cttgtgtcag ccacggtcta ttttgagtgt cgacctgaca ccagtaactc ctggggcttc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gccaggctgc ccgcgacccc acttcactcc taggaggggt cttcacgatc ctattgtaga 900

841 cggtccgacg ggcgctgggg tgaagtgagg atcctcccca gaagtgctag gataacatct 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgggggccct tgttcgcacc ctgagctcgt gctgctgttc tgggatacgc catcacggct 960

901 acccccggga acaagcgtgg gactcgagca cgacgacaag accctatgcg gtagtgccga 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgccgatcgc acggcacccg aggcccacgg ccctaaccca gcctgacccc agtcctggcc 1020

961 acggctagcg tgccgtgggc tccgggtgcc gggattgggt cggactgggg tcaggaccgg 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcggcccgtg tgctgttccc agggcccacc tcccctccat atgggagtgc ccgggaccac 1080

1021 agccgggcac acgacaaggg tcccgggtgg aggggaggta taccctcacg ggccctggtg 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 acctccttgc tcaccgcagg gatctggtgc ccggccaagg cttggccccg ttcacctcct 1140

1081 tggaggaacg agtggcgtcc ctagaccacg ggccggttcc gaaccggggc aagtggagga 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ggagacgagg ccttgcttca ggagccctaa gagcttctgt cccctccccg ctttggttcc 1200

1141 cctctgctcc ggaacgaagt cctcgggatt ctcgaagaca ggggaggggc gaaaccaagg 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctctgccctg gtcccttcct ccaccatcgc tcccttcagc tcatccttga gggcttttga 1260

1201 gagacgggac cagggaagga ggtggtagcg agggaagtcg agtaggaact cccgaaaact 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gccaccttcc tctccccaag atctgcccaa gttcccctgg ataaagccct ggcactaagc 1320

1261 cggtggaagg agaggggttc tagacgggtt caaggggacc tatttcggga ccgtgattcg 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cccaccccag ctgaccctag cccacgaggg cccacatccc agatgggcct cgggccttca 1380

1321 gggtggggtc gactgggatc gggtgctccc gggtgtaggg tctacccgga gcccggaagt 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccgggcctct gtcttcagtc tatgctgact tccccacttg gcaacctggc aaagtgcccc 1440

1381 ggcccggaga cagaagtcag atacgactga aggggtgaac cgttggaccg tttcacgggg 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cctccctcaa cacattgtcc ccctggacgt ctctgcagat aggtggcctc cccgccccgg 1500

1441 ggagggagtt gtgtaacagg gggacctgca gagacgtcta tccaccggag gggcggggcc 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gtccagagcg ggtcttccca cacggaagtc ctcctcagcc cataagctcc tccttcctgg 1560

1501 caggtctcgc ccagaagggt gtgccttcag gaggagtcgg gtattcgagg aggaaggacc 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 agcctcgggt gccccagtgg cctgcacata aatgtcggtt tgaacaagtg agtgagtgaa 1620

1561 tcggagccca cggggtcacc ggacgtgtat ttacagccaa acttgttcac tcactcactt 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ctccagttca agggaacaaa tgctttttga gctcctgggc aacgggggct tgtgtccggt 1680

1621 gaggtcaagt tcccttgttt acgaaaaact cgaggacccg ttgcccccga acacaggcca 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ccccggagcg ccccgctgca cttgtgaagc tcacgacctg gccggtgggc actgtcagtg 1740

1681 ggggcctcgc ggggcgacgt gaacacttcg agtgctggac cggccacccg tgacagtcac 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tgtggtgact ggataagccc agaggtgacc caggtgggga agggacagct caggggctga 1800

1741 acaccactga cctattcggg tctccactgg gtccacccct tccctgtcga gtccccgact 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gtgaggggat gaccgaagga ccagcgctct gccctccagg agctcgtggg ccaggcggga 1860

1801 cactccccta ctggcttcct ggtcgcgaga cgggaggtcc tcgagcaccc ggtccgccct 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ggaaggcaaa atttgcttgc cctgagccag agggaagtgg gcctagagct aggggtccca 1920

1861 ccttccgttt taaacgaacg ggactcggtc tcccttcacc cggatctcga tccccagggt 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 atgccaggcc aggcccatca gctgagatgc cagaggggga caggggagtt gggggcaggc 1980

1921 tacggtccgg tccgggtagt cgactctacg gtctccccct gtcccctcaa cccccgtccg 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ctctgctctt tcccagggtc cccagcacgc cccgccttct ccccgaccac agccttctac 2040

1981 gagacgagaa agggtcccag gggtcgtgcg gggcggaaga ggggctggtg tcggaagatg 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ctggagtcac cttggcccca cctggaagcc aattaagccc ctcgttgcag cagtgaggat 2100

2041 gacctcagtg gaaccggggt ggaccttcgg ttaattcggg gagcaacgtc gtcactccta 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 taatatgatt aataacgccc ccaatctccg aggtgctgat tcagccggga gcttagggag 2160

2101 attatactaa ttattgcggg ggttagaggc tccacgacta agtcggccct cgaatccctc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gggaggtcac ttcataaggg cttggggggg gagttggagc cacgagtcgt ccagccggag 2220

2161 ccctccagtg aagtattccc gaaccccccc ctcaacctcg gtgctcagca ggtcggcctc 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ccccgtgtgg ctgagctccg gcctcagaag catccccggg ttcgcgccgc cggggatcct 2280

2221 ggggcacacc gactcgaggc cggagtcttc gtaggggccc aagcgcggcg gcccctagga 2280

.......... .......... .......... .......... .......... ..........

.......... ......... EcoRI(2302) ......... .......... ..........

2281 ctagagtcga gctcaagctt cgaattctga gccgccacca tggccaattt actgaccgta 2340

2281 gatctcagct cgagttcgaa gcttaagact cggcggtggt accggttaaa tgactggcat 2340

.......... .......... .......... .......... ORF 1 CRE_NLS

.......... .......... .......... .......... .......... ..........

2341 caccaaaatt tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg 2400

2341 gtggttttaa acggacgtaa tggccagcta cgttgctcac tactccaagc gttcttggac 2400

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 atggacatgt tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc 2460

2401 tacctgtaca agtccctagc ggtccgcaaa agactcgtat ggacctttta cgaagacagg 2460

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtttgccggt cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa 2520

2461 caaacggcca gcacccgccg taccacgttc aacttattgg cctttaccaa agggcgtctt 2520

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... NruI(2534) .......... .......... .......... ..........

2521 cctgaagatg ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact 2580

2521 ggacttctac aagcgctaat agaagatata gaagtccgcg cgccagaccg tcatttttga 2580

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 atccagcaac atttgggcca gctaaacatg cttcatcgtc ggtccgggct gccacgacca 2640

2581 taggtcgttg taaacccggt cgatttgtac gaagtagcag ccaggcccga cggtgctggt 2640

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agtgacagca atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc 2700

2641 tcactgtcgt tacgacaaag tgaccaatac gccgcctagg cttttctttt gcaactacgg 2700

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca 2760

2701 ccacttgcac gttttgtccg agatcgcaag cttgcgtgac taaagctggt ccaagcaagt 2760

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ctcatggaaa atagcgatcg ctgccaggat atacgtaatc tggcatttct ggggattgct 2820

2761 gagtaccttt tatcgctagc gacggtccta tatgcattag accgtaaaga cccctaacga 2820

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... ......... EcoRV(2871)

2821 tataacaccc tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt 2880

2821 atattgtggg acaatgcata tcggctttaa cggtcctagt cccaatttct atagagtgca 2880

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 actgacggtg ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca 2940

2881 tgactgccac cctcttacaa ttaggtataa ccgtcttgct tttgcgacca atcgtggcgt 2940

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggtgtagaga aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc 3000

2941 ccacatctct tccgtgaatc ggacccccat tgatttgacc agctcgctac ctaaaggcag 3000

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tctggtgtag ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt 3060

3001 agaccacatc gactactagg cttattgatg gacaaaacgg cccagtcttt tttaccacaa 3060

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gccgcgccat ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca 3120

3061 cggcgcggta gacggtggtc ggtcgatagt tgagcgcggg accttcccta aaaacttcgt 3120

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

ClaI(3126) .......... .......... .......... .......... ..........

3121 actcatcgat tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct 3180

3121 tgagtagcta actaaatgcc gcgattccta ctgagaccag tctctatgga ccggaccaga 3180

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ggacacagtg cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg 3240

3181 cctgtgtcac gggcacagcc tcggcgcgct ctataccggg cgcgacctca aagttatggc 3240

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gagatcatgc aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac 3300

3241 ctctagtacg ttcgaccacc gacctggtta catttataac agtacttgat ataggcattg 3300

.......... ........ CRE_NLS ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctggatagtg aaacaggggc aatggtgcgc ctgctggaag atggcgatgg accggtggaa 3360

3301 gacctatcac tttgtccccg ttaccacgcg gacgaccttc taccgctacc tggccacctt 3360

.......... CRE_NLS ORF 1 .......... .......... ..........

.......... .......... .... NotI(3394),EagI(3394) .... ..........

3361 caaaaactta tttctgaaga agatctgtga tagcggccgc actcctcagg tgcaggctgc 3420

3361 gtttttgaat aaagacttct tctagacact atcgccggcg tgaggagtcc acgtccgacg 3420

.......... ORF 1 .......... .......... ....... rb_glob_PA_term

.......... .......... .......... .......... .......... ..........

3421 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct 3480

3421 gatagtcttc caccaccgac cacaccggtt acgggaccga gtgtttatgg tgactctaga 3480

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg 3540

3481 aaaagggaga cggtttttaa tacccctgta gtacttcggg gaactcgtag actgaagacc 3540

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact 3600

3541 gattatttcc tttaaataaa agtaacgtta tcacacaacc ttaaaaaaca cagagagtga 3600

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag 3660

3601 gccttcctgt ataccctccc gtttagtaaa ttttgtagtc ttactcataa accaaatctc 3660

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tttggcaaca tatgccatat gctggctgcc atgaacaaag gtggctataa agaggtcatc 3720

3661 aaaccgttgt atacggtata cgaccgacgg tacttgtttc caccgatatt tctccagtag 3720

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag 3780

3721 tcatatactt tgtcggggga cgacaggtaa ggaataaggt atcttttcgg aactgaactc 3780

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt 3840

3781 caatctaaaa aaaatataaa acaaaacaca ataaaaaaag aaattgtagg gattttaaaa 3840

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg 3900

3841 ggaatgtaca aaatgatcgg tctaaaaagg aggagaggac tgatgagggt cagtatcgac 3900

.......... ....... rb_glob_PA_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tccctcttct cttatgaaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca 3960

3901 agggagaaga gaatacttct agggagctgg acgtcgggtt cgaaccgcat tagtaccagt 3960

rb_glob_PA_term .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 4020

3961 atcgacaaag gacacacttt aacaataggc gagtgttaag gtgtgttgta tgctcggcct 4020

M13_reverse_pri . M13_pUC_rev_pri ....... .......... lac_pro

.......... .......... .......... .......... .......... ..........

4021 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 4080

4021 tcgtatttca catttcggac cccacggatt actcactcga ttgagtgtaa ttaacgcaac 4080

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt 4140

4081 gcgagtgacg ggcgaaaggt cagccctttg gacagcacgg tcgcctaggc gtagagttaa 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact c cgcccagtt 4200

4141 tcagtcgttg gtatcagggc ggggattgag gcgggtaggg cggggattga ggcgggtcaa 4200

.......... .......... SV40_pro ......... SV40_origin .........

.......... .......... .......... .......... .......... ..........

4201 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 4260

4201 ggcgggtaag aggcggggta ccgactgatt aaaaaaaata aatacgtctc cggctccggc 4260

.......... ..... SV40_pro SV40_origin ... SV40pro_F_pri ........

.......... .......... .......... .......... .......... ..........

4261 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 4320

4261 ggagccggag actcgataag gtcttcatca ctcctccgaa aaaacctccg gatccgaaaa 4320

.......... .......... SV40_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 4380

4321 cgtttttcga ttgaacaaat aacgtcgaat attaccaatg tttatttcgt tatcgtagtg 4380

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

4381 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 4440

4381 tttaaagtgt ttatttcgta aaaaaagtga cgtaagatca acaccaaaca ggtttgagta 4440

.......... ......... SV40_PA_term ......... ......... EBV_rev_pri

.......... .......... .......... .......... .......... ..........

4441 caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga 4500

4441 gttacataga atagtacaga cctaggcgac gtaattactt agccggttgc gcgcccctct 4500

......... SV40_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 4560

4501 ccgccaaacg cataacccgc gagaaggcga aggagcgagt gactgagcga cgcgagccag 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 4620

4561 caagccgacg ccgctcgcca tagtcgagtg agtttccgcc attatgccaa taggtgtctt 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 4680

4621 agtcccctat tgcgtccttt cttgtacact cgttttccgg tcgttttccg gtccttggca 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 4740

4681 tttttccggc gcaacgaccg caaaaaggta tccgaggcgg ggggactgct cgtagtgttt 4740

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

4741 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 4800

4741 ttagctgcga gttcagtctc caccgctttg ggctgtcctg atatttctat ggtccgcaaa 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 4860

4801 gggggacctt cgagggagca cgcgagagga caaggctggg acggcgaatg gcctatggac 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc 4920

4861 aggcggaaag agggaagccc ttcgcaccgc gaaagagtta cgagtgcgac atccatagag 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 4980

4921 tcaagccaca tccagcaagc gaggttcgac ccgacacacg tgcttggggg gcaagtcggg 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 5040

4981 ctggcgacgc ggaataggcc attgatagca gaactcaggt tgggccattc tgtgctgaat 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 5100

5041 agcggtgacc gtcgtcggtg accattgtcc taatcgtctc gctccataca tccgccacga 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 5160

5101 tgtctcaaga acttcaccac cggattgatg ccgatgtgat cttcctgtca taaaccatag 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 5220

5161 acgcgagacg acttcggtca atggaagcct ttttctcaac catcgagaac taggccgttt 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 5280

5221 gtttggtggc gaccatcgcc accaaaaaaa caaacgttcg tcgtctaatg cgcgtctttt 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 5340

5281 tttcctagag ttcttctagg aaactagaaa agatgcccca gactgcgagt caccttgctt 5340

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 5400

5341 ttgagtgcaa ttccctaaaa ccagtactct aatagttttt cctagaagtg gatctaggaa 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 5460

5401 aatttaattt ttacttcaaa atttagttag atttcatata tactcatttg aaccagactg 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 5520

5461 tcaatggtta cgaattagtc actccgtgga tagagtcgct agacagataa agcaagtagg 5520

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

5521 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 5580

5521 tatcaacgga ctgaggggca gcacatctat tgatgctatg ccctcccgaa tggtagaccg 5580

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 5640

5581 gggtcacgac gttactatgg cgctctgggt gcgagtggcc gaggtctaaa tagtcgttat 5640

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 5700

5641 ttggtcggtc ggccttcccg gctcgcgtct tcaccaggac gttgaaatag gcggaggtag 5700

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... FspI(5758)

5701 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 5760

5701 gtcagataat taacaacggc ccttcgatct cattcatcaa gcggtcaatt atcaaacgcg 5760

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 5820

5761 ttgcaacaac ggtaacgatg tccgtagcac cacagtgcga gcagcaaacc ataccgaagt 5820

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 5880

5821 aagtcgaggc caagggttgc tagttccgct caatgtacta gggggtacaa cacgtttttt 5880

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 5940

5881 cgccaatcga ggaagccagg aggctagcaa cagtcttcat tcaaccggcg tcacaatagt 5940

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 6000

5941 gagtaccaat accgtcgtga cgtattaaga gaatgacagt acggtaggca ttctacgaaa 6000

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 6060

6001 agacactgac cactcatgag ttggttcagt aagactctta tcacatacgc cgctggctca 6060

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 6120

6061 acgagaacgg gccgcagtta tgccctatta tggcgcggtg tatcgtcttg aaattttcac 6120

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 6180

6121 gagtagtaac cttttgcaag aagccccgct tttgagagtt cctagaatgg cgacaactct 6180

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 6240

6181 aggtcaagct acattgggtg agcacgtggg ttgactagaa gtcgtagaaa atgaaagtgg 6240

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 6300

6241 tcgcaaagac ccactcgttt ttgtccttcc gttttacggc gttttttccc ttattcccgc 6300

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 6360

6301 tgtgccttta caacttatga gtatgagaag gaaaaagtta taataacttc gtaaatagtc 6360

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 6420

6361 ccaataacag agtactcgcc tatgtataaa cttacataaa tctttttatt tgtttatccc 6420

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 gttccgcgca catttccccg aaaagtgcca cctg...... .......... .......... 6454

6421 caaggcgcgt gtaaaggggc ttttcacggt ggac...... .......... .......... 6454

.......... .......... .......... .......... .......... ..........