Sequence of pShuttle-CMV-EGFP-CA

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Sequence of pShuttle-CMV-EGFP-CA

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Sequence of pShuttle-CMV-EGFP-CA : 8212 BP; 2032 A; 2121 C; 2188 G; 1844 T; 27 other;

.......... .......... .......... .......... .......... ..........

1 ttaattaann ntcccttcca gctctctgcc ccttttggat tgaagccaat atgataatga 60

1 aattaattnn nagggaaggt cgagagacgg ggaaaaccta acttcggtta tactattact 60

.......... .......... .......... .......... RITR ..........

.......... .......... .......... .......... .......... ..........

61 gggggtggag tttgtgacgt ggcgcggggc gtgggaacgg ggcgggtgac gtagtagtgt 120

61 cccccacctc aaacactgca ccgcgccccg cacccttgcc ccgcccactg catcatcaca 120

.......... .......... RITR LITR .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggcggaagtg tgatgttgca agtgtggcgg aacacatgta agcgacggat gtggcaaaag 180

121 ccgccttcac actacaacgt tcacaccgcc ttgtgtacat tcgctgccta caccgttttc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tgacgttttt ggtgtgcgcc ggtgtacaca ggaagtgaca attttcgcgc ggttttaggc 240

181 actgcaaaaa ccacacgcgg ccacatgtgt ccttcactgt taaaagcgcg ccaaaatccg 240

.......... .......... .......... Encap .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggatgttgta gtaaatttgg gcgtaaccga gtaagatttg gccattttcg cgggaaaact 300

241 cctacaacat catttaaacc cgcattggct cattctaaac cggtaaaagc gcccttttga 300

.......... .......... Encap .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gaataagagg aagtgaaatc tgaataattt tgtgttactc atagcgcgta annnntaata 360

301 cttattctcc ttcactttag acttattaaa acacaatgag tatcgcgcat tnnnnattat 360

.......... .......... Encap .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 420

361 cattagttaa tgccccagta atcaagtatc gggtatatac ctcaaggcgc aatgtattga 420

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 480

421 atgccattta ccgggcggac cgactggcgg gttgctgggg gcgggtaact gcagttatta 480

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 540

481 ctgcatacaa gggtatcatt gcggttatcc ctgaaaggta actgcagtta cccacctcat 540

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... NdeI(582) ..........

541 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 600

541 aaatgccatt tgacgggtga accgtcatgt agttcacata gtatacggtt catgcggggg 600

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 660

601 ataactgcag ttactgccat ttaccgggcg gaccgtaata cgggtcatgt actggaatac 660

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 720

661 cctgaaagga tgaaccgtca tgtagatgca taatcagtag cgataatggt accactacgc 720

......... CAG_enh CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 780

721 caaaaccgtc atgtagttac ccgcacctat cgccaaactg agtgccccta aaggttcaga 780

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 840

781 ggtggggtaa ctgcagttac cctcaaacaa aaccgtggtt ttagttgccc tgaaaggttt 840

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 900

841 tacagcattg ttgaggcggg gtaactgcgt ttacccgcca tccgcacatg ccaccctcca 900

.......... ........ CMV_imm_pro CMV_fwd_pri ........ CMV_pro

.......... .......... .......... .......... ClaI(947) ..........

901 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagagatcga tctggtaccg 960

901 gatatattcg tctcgaccaa atcacttggc agtctaggcg atctctagct agaccatggc 960

CMV_imm_pro CMV_pro ........ .......... .......... ..........

NotI(970) .......... .......... .......... .......... ..........

961 tcgacgtcgc ggccgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc 1020

961 agctgcagcg ccggcggtgg taccactcgt tcccgctcct cgacaagtgg ccccaccacg 1020

.......... .......... .......... EGFP ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 1080

1021 ggtaggacca gctcgacctg ccgctgcatt tgccggtgtt caagtcgcac aggccgctcc 1080

.......... EGFP_N_pri EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 1140

1081 cgctcccgct acggtggatg ccgttcgact gggacttcaa gtagacgtgg tggccgttcg 1140

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc 1200

1141 acgggcacgg gaccgggtgg gagcactggt gggactggat gccgcacgtc acgaagtcgg 1200

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 1260

1201 cgatggggct ggtgtacttc gtcgtgctga agaagttcag gcggtacggg cttccgatgc 1260

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 1320

1261 aggtcctcgc gtggtagaag aagttcctgc tgccgttgat gttctgggcg cggctccact 1320

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 1380

1321 tcaagctccc gctgtgggac cacttggcgt agctcgactt cccgtagctg aagttcctcc 1380

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 1440

1381 tgccgttgta ggaccccgtg ttcgacctca tgttgatgtt gtcggtgttg cagatatagt 1440

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 1500

1441 accggctgtt cgtcttcttg ccgtagttcc acttgaagtt ctaggcggtg ttgtagctcc 1500

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 1560

1501 tgccgtcgca cgtcgagcgg ctggtgatgg tcgtcttgtg ggggtagccg ctgccggggc 1560

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 1620

1561 acgacgacgg gctgttggtg atggactcgt gggtcaggcg ggactcgttt ctggggttgc 1620

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 1680

1621 tcttcgcgct agtgtaccag gacgacctca agcactggcg gcggccctag tgagagccgt 1680

.......... .... EGFP ORF 3 EGFP_C_pri .... .......... ..........

.......... ......... BglII(1707) ......... .......... ..........

1681 tggacgagct gtacaagtcc ggactcagat ctgtaggtgc attaatggaa gtgatgcaga 1740

1681 acctgctcga catgttcagg cctgagtcta gacatccacg taattacctt cactacgtct 1740

EGFP .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 agaggagcaa agccattcat tcttcagacg aagatgagga tgaagatgat gatgaagatt 1800

1741 tctcctcgtt tcggtaagta agaagtctgc ttctactcct acttctacta ctacttctaa 1800

.......... .......... ORF 3 .......... .......... ..........

.......... ........ HindIII(1830) ....... EcoRV(1846) .........

1801 ttgaggatga tgatgaatgg gaagactgaa agcttctaga taagatatcc gatccaccgg 1860

1801 aactcctact actacttacc cttctgactt tcgaagatct attctatagg ctaggtggcc 1860

.......... ORF 3 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 1920

1861 tagatctatt gactagtatt agtcggtatg gtgtaaacat ctccaaaatg aacgaaattt 1920

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... HpaI(1978)

1921 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 1980

1921 tttggagggt gtggaggggg acttggactt tgtattttac ttacgttaac aacaacaatt 1980

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 2040

1981 gaacaaataa cgtcgaatat taccaatgtt tatttcgtta tcgtagtgtt taaagtgttt 2040

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 2100

2041 atttcgtaaa aaaagtgacg taagatcaac accaaacagg tttgagtagt tacatagaat 2100

.......... ......... SV40_PA_term ........ EBV_rev_pri .........

.......... .......... .......... .......... .......... ..........

2101 acgcnnnnta agggtgggaa agaatatata aggtgggggt cttatgtagt tttgtatctg 2160

2101 tgcgnnnnat tcccaccctt tcttatatat tccaccccca gaatacatca aaacatagac 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ttttgcagca gccgccgccg ccatgagcac caactcgttt gatggaagca ttgtgagctc 2220

2161 aaaacgtcgt cggcggcggc ggtactcgtg gttgagcaaa ctaccttcgt aacactcgag 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 atatttgaca acgcgcatgc ccccatgggc cggggtgcgt cagaatgtga tgggctccag 2280

2221 tataaactgt tgcgcgtacg ggggtacccg gccccacgca gtcttacact acccgaggtc 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cattgatggt cgccccgtcc tgcccgcaaa ctctactacc ttgacctacg agaccgtgtc 2340

2281 gtaactacca gcggggcagg acgggcgttt gagatgatgg aactggatgc tctggcacag 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tggaacgccg ttggagactg cagcctccgc cgccgcttca gccgctgcag ccaccgcccg 2400

2341 accttgcggc aacctctgac gtcggaggcg gcggcgaagt cggcgacgtc ggtggcgggc 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cgggattgtg actgactttg ctttcctgag cccgcttgca agcagtgcag cttcccgttc 2460

2401 gccctaacac tgactgaaac gaaaggactc gggcgaacgt tcgtcacgtc gaagggcaag 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 atccgcccgc gatgacaagt tgacggctct tttggcacaa ttggattctt tgacccggga 2520

2461 taggcgggcg ctactgttca actgccgaga aaaccgtgtt aacctaagaa actgggccct 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 acttaatgtc gtttctcagc agctgttgga tctgcgccag caggtttctg ccctgaaggc 2580

2521 tgaattacag caaagagtcg tcgacaacct agacgcggtc gtccaaagac gggacttccg 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttcctcccct cccaatgcgg tttaaaacat aaataaaaaa ccagactctg tttggatttg 2640

2581 aaggagggga gggttacgcc aaattttgta tttatttttt ggtctgagac aaacctaaac 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gatcaagcaa gtgtcttgct gtctttattt aggggttttg cgcgcgcggt aggcccggga 2700

2641 ctagttcgtt cacagaacga cagaaataaa tccccaaaac gcgcgcgcca tccgggccct 2700

.......... .......... .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

2701 ccagcggtct cggtcgttga gggtcctgtg tattttttcc aggacgtggt aaaggtgact 2760

2701 ggtcgccaga gccagcaact cccaggacac ataaaaaagg tcctgcacca tttccactga 2760

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ctggatgttc agatacatgg gcataagccc gtctctgggg tggaggtagc accactgcag 2820

2761 gacctacaag tctatgtacc cgtattcggg cagagacccc acctccatcg tggtgacgtc 2820

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 agcttcatgc tgcggggtgg tgttgtagat gatccagtcg tagcaggagc gctgggcgtg 2880

2821 tcgaagtacg acgccccacc acaacatcta ctaggtcagc atcgtcctcg cgacccgcac 2880

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gtgcctaaaa atgtctttca gtagcaagct gattgccagg ggcaggccct tggtgtaagt 2940

2881 cacggatttt tacagaaagt catcgttcga ctaacggtcc ccgtccggga accacattca 2940

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gtttacaaag cggttaagct gggatgggtg catacgtggg gatatgagat gcatcttgga 3000

2941 caaatgtttc gccaattcga ccctacccac gtatgcaccc ctatactcta cgtagaacct 3000

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctgtattttt aggttggcta tgttcccagc catatccctc cggggattca tgttgtgcag 3060

3001 gacataaaaa tccaaccgat acaagggtcg gtatagggag gcccctaagt acaacacgtc 3060

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aaccaccagc acagtgtatc cggtgcactt gggaaatttg tcatgtagct tagaaggaaa 3120

3061 ttggtggtcg tgtcacatag gccacgtgaa ccctttaaac agtacatcga atcttccttt 3120

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tgcgtggaag aacttggaga cgcccttgtg acctccaaga ttttccatgc attcgtccat 3180

3121 acgcaccttc ttgaacctct gcgggaacac tggaggttct aaaaggtacg taagcaggta 3180

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aatgatggca atgggcccac gggcggcggc ctgggcgaag atatttctgg gatcactaac 3240

3181 ttactaccgt tacccgggtg cccgccgccg gacccgcttc tataaagacc ctagtgattg 3240

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gtcatagttg tgttccagga tgagatcgtc ataggccatt tttacaaagc gcgggcggag 3300

3241 cagtatcaac acaaggtcct actctagcag tatccggtaa aaatgtttcg cgcccgcctc 3300

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggtgccagac tgcggtataa tggttccatc cggcccaggg gcgtagttac cctcacagat 3360

3301 ccacggtctg acgccatatt accaaggtag gccgggtccc cgcatcaatg ggagtgtcta 3360

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttgcatttcc cacgctttga gttcagatgg ggggatcatg tctacctgcg gggcgatgaa 3420

3361 aacgtaaagg gtgcgaaact caagtctacc cccctagtac agatggacgc cccgctactt 3420

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gaaaacggtt tccggggtag gggagatcag ctgggaagaa agcaggttcc tgagcagctg 3480

3421 cttttgccaa aggccccatc ccctctagtc gacccttctt tcgtccaagg actcgtcgac 3480

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgacttaccg cagccggtgg gcccgtaaat cacacctatt accgggtgca actggtagtt 3540

3481 gctgaatggc gtcggccacc cgggcattta gtgtggataa tggcccacgt tgaccatcaa 3540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aagagagctg cagctgccgt catccctgag caggggggcc acttcgttaa gcatgtccct 3600

3541 ttctctcgac gtcgacggca gtagggactc gtccccccgg tgaagcaatt cgtacaggga 3600

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gactcgcatg ttttccctga ccaaatccgc cagaaggcgc tcgccgccca gcgatagcag 3660

3601 ctgagcgtac aaaagggact ggtttaggcg gtcttccgcg agcggcgggt cgctatcgtc 3660

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttcttgcaag gaagcaaagt ttttcaacgg tttgagaccg tccgccgtag gcatgctttt 3720

3661 aagaacgttc cttcgtttca aaaagttgcc aaactctggc aggcggcatc cgtacgaaaa 3720

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gagcgtttga ccaagcagtt ccaggcggtc ccacagctcg gtcacctgct ctacggcatc 3780

3721 ctcgcaaact ggttcgtcaa ggtccgccag ggtgtcgagc cagtggacga gatgccgtag 3780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tcgatccagc atatctcctc gtttcgcggg ttggggcggc tttcgctgta cggcagtagt 3840

3781 agctaggtcg tatagaggag caaagcgccc aaccccgccg aaagcgacat gccgtcatca 3840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cggtgctcgt ccagacgggc cagggtcatg tctttccacg ggcgcagggt cctcgtcagc 3900

3841 gccacgagca ggtctgcccg gtcccagtac agaaaggtgc ccgcgtccca ggagcagtcg 3900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gtagtctggg tcacggtgaa ggggtgcgct ccgggctgcg cgctggccag ggtgcgcttg 3960

3901 catcagaccc agtgccactt ccccacgcga ggcccgacgc gcgaccggtc ccacgcgaac 3960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 aggctggtcc tgctggtgct gaagcgctgc cggtcttcgc cctgcgcgtc ggccaggtag 4020

3961 tccgaccagg acgaccacga cttcgcgacg gccagaagcg ggacgcgcag ccggtccatc 4020

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 catttgacca tggtgtcata gtccagcccc tccgcggcgt ggcccttggc gcgcagcttg 4080

4021 gtaaactggt accacagtat caggtcgggg aggcgccgca ccgggaaccg cgcgtcgaac 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cccttggagg aggcgccgca cgaggggcag tgcagacttt tgagggcgta gagcttgggc 4140

4081 gggaacctcc tccgcggcgt gctccccgtc acgtctgaaa actcccgcat ctcgaacccg 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gcgagaaata ccgattccgg ggagtaggca tccgcgccgc aggccccgca gacggtctcg 4200

4141 cgctctttat ggctaaggcc cctcatccgt aggcgcggcg tccggggcgt ctgccagagc 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cattccacga gccaggtgag ctctggccgt tcggggtcaa aaaccaggtt tcccccatgc 4260

4201 gtaaggtgct cggtccactc gagaccggca agccccagtt tttggtccaa agggggtacg 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tttttgatgc gtttcttacc tctggtttcc atgagccggt gtccacgctc ggtgacgaaa 4320

4261 aaaaactacg caaagaatgg agaccaaagg tactcggcca caggtgcgag ccactgcttt 4320

.......... .......... .......... .......... .......... ..........

.......... .......... ......... EcoRI(4360) ......... ..........

4321 aggctgtccg tgtccccgta tacagactnn ngtttaaacg aattcnnnat ataaaatgca 4380

4321 tccgacaggc acaggggcat atgtctgann ncaaatttgc ttaagnnnta tattttacgt 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aggtgctgct caaaaaatca ggcaaagcct cgcgcaaaaa agaaagcaca tcgtagtcat 4440

4381 tccacgacga gttttttagt ccgtttcgga gcgcgttttt tctttcgtgt agcatcagta 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gctcatgcag ataaaggcag gtaagctccg gaaccaccac agaaaaagac accatttttc 4500

4441 cgagtacgtc tatttccgtc cattcgaggc cttggtggtg tctttttctg tggtaaaaag 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tctcaaacat gtctgcgggt ttctgcataa acacaaaata aaataacaaa aaaacattta 4560

4501 agagtttgta cagacgccca aagacgtatt tgtgttttat tttattgttt ttttgtaaat 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 aacattagaa gcctgtctta caacaggaaa aacaaccctt ataagcataa gacggactac 4620

4561 ttgtaatctt cggacagaat gttgtccttt ttgttgggaa tattcgtatt ctgcctgatg 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggccatgccg gcgtgaccgt aaaaaaactg gtcaccgtga ttaaaaagca ccaccgacag 4680

4621 ccggtacggc cgcactggca tttttttgac cagtggcact aatttttcgt ggtggctgtc 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ctcctcggtc atgtccggag tcataatgta agactcggta aacacatcag gttgattcat 4740

4681 gaggagccag tacaggcctc agtattacat tctgagccat ttgtgtagtc caactaagta 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cggtcagtgc taaaaagcga ccgaaatagc ccgggggaat acatacccgc aggcgtagag 4800

4741 gccagtcacg atttttcgct ggctttatcg ggccccctta tgtatgggcg tccgcatctc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 acaacattac agcccccata ggaggtataa caaaattaat aggagagaaa aacacataaa 4860

4801 tgttgtaatg tcgggggtat cctccatatt gttttaatta tcctctcttt ttgtgtattt 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cacctgaaaa accctcctgc ctaggcaaaa tagcaccctc ccgctccaga acaacataca 4920

4861 gtggactttt tgggaggacg gatccgtttt atcgtgggag ggcgaggtct tgttgtatgt 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gcgcttcaca gcggcagcct aacagtcagc cttaccagta aaaaagaaaa cctattaaaa 4980

4921 cgcgaagtgt cgccgtcgga ttgtcagtcg gaatggtcat tttttctttt ggataatttt 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aaacaccact cgacacggca ccagctcaat cagtcacagt gtaaaaaagg gccaagtgca 5040

4981 tttgtggtga gctgtgccgt ggtcgagtta gtcagtgtca cattttttcc cggttcacgt 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gagcgagtat atataggact aaaaaatgac gtaacggtta aagtccacaa aaaacaccca 5100

5041 ctcgctcata tatatcctga ttttttactg cattgccaat ttcaggtgtt ttttgtgggt 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gaaaaccgca cgcgaaccta cgcccagaaa cgaaagccaa aaaacccaca acttcctcaa 5160

5101 cttttggcgt gcgcttggat gcgggtcttt gctttcggtt ttttgggtgt tgaaggagtt 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 atcgtcactt ccgttttccc acgttacgta acttcccatt ttaagaaaac tacaattccc 5220

5161 tagcagtgaa ggcaaaaggg tgcaatgcat tgaagggtaa aattcttttg atgttaaggg 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 aacacataca agttactccg ccctaaaacc tacgtcaccc gccccgttcc cacgccccgc 5280

5221 ttgtgtatgt tcaatgaggc gggattttgg atgcagtggg cggggcaagg gtgcggggcg 5280

.......... .......... .......... .......... LITR ..........

.......... .......... .......... .......... .......... ..........

5281 gccacgtcac aaactccacc ccctcattat catattggct tcaatccaaa ataaggtata 5340

5281 cggtgcagtg tttgaggtgg gggagtaata gtataaccga agttaggttt tattccatat 5340

.......... .......... LITR RITR .......... ..........

.......... ......... BamHI(5363) ......... .......... ..........

5341 ttattgatga tnnnttaatt aaggatccnn ncggtgtgaa ataccgcaca gatgcgtaag 5400

5341 aataactact annnaattaa ttcctaggnn ngccacactt tatggcgtgt ctacgcattc 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 5460

5401 ctcttttatg gcgtagtccg cgagaaggcg aaggagcgag tgactgagcg acgcgagcca 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 5520

5461 gcaagccgac gccgctcgcc atagtcgagt gagtttccgc cattatgcca ataggtgtct 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 5580

5521 tagtccccta ttgcgtcctt tcttgtacac tcgttttccg gtcgttttcc ggtccttggc 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 5640

5581 atttttccgg cgcaacgacc gcaaaaaggt atccgaggcg gggggactgc tcgtagtgtt 5640

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

5641 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 5700

5641 tttagctgcg agttcagtct ccaccgcttt gggctgtcct gatatttcta tggtccgcaa 5700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 5760

5701 agggggacct tcgagggagc acgcgagagg acaaggctgg gacggcgaat ggcctatgga 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 5820

5761 caggcggaaa gagggaagcc cttcgcaccg cgaaagagta tcgagtgcga catccataga 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 5880

5821 gtcaagccac atccagcaag cgaggttcga cccgacacac gtgcttgggg ggcaagtcgg 5880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5881 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 5940

5881 gctggcgacg cggaataggc cattgatagc agaactcagg ttgggccatt ctgtgctgaa 5940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5941 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 6000

5941 tagcggtgac cgtcgtcggt gaccattgtc ctaatcgtct cgctccatac atccgccacg 6000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6001 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 6060

6001 atgtctcaag aacttcacca ccggattgat gccgatgtga tcttcctgtc ataaaccata 6060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 6120

6061 gacgcgagac gacttcggtc aatggaagcc tttttctcaa ccatcgagaa ctaggccgtt 6120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6121 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 6180

6121 tgtttggtgg cgaccatcgc caccaaaaaa acaaacgttc gtcgtctaat gcgcgtcttt 6180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6181 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 6240

6181 ttttcctaga gttcttctag gaaactagaa aagatgcccc agactgcgag tcaccttgct 6240

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 6300

6241 tttgagtgca attccctaaa accagtactc taatagtttt tcctagaagt ggatctagga 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 6360

6301 aaatttaatt tttacttcaa aatttagtta gatttcatat atactcattt gaaccagact 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 6420

6361 gtcaatggtt acgaattagt cactccgtgg atagagtcgc tagacagata aagcaagtag 6420

.......... .......... .......... Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

6421 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 6480

6421 gtatcaacgg actgaggggc agcacatcta ttgatgctat gccctcccga atggtagacc 6480

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 6540

6481 ggggtcacga cgttactatg gcgctctggg tgcgagtggc cgaggtctaa atagtcgtta 6540

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 6600

6541 tttggtcggt cggccttccc ggctcgcgtc ttcaccagga cgttgaaata ggcggaggta 6600

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 6660

6601 ggtcagataa ttaacaacgg cccttcgatc tcattcatca agcggtcaat tatcaaacgc 6660

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 caacgttgtt gnnnnaaaaa ggatcttcac ctagatcctt ttcacgtaga aagccagtcc 6720

6661 gttgcaacaa cnnnnttttt cctagaagtg gatctaggaa aagtgcatct ttcggtcagg 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa 6780

6721 cgtctttgcc acgactgggg cctacttaca gtcgatgacc cgatagacct gttccctttt 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg 6840

6781 gcgttcgcgt ttctctttcg tccatcgaac gtcacccgaa tgtaccgcta tcgatctgac 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt 6900

6841 ccgccaaaat acctgtcgtt cgcttggcct taacggtcga ccccgcggga gaccattcca 6900

.......... .......... .......... .......... NEOKAN_pro ..........

.......... .......... .......... .......... .......... ..........

6901 tgggaagccc tgcaaagtaa actggatggc tttctcgccg ccaaggatct gatggcgcag 6960

6901 acccttcggg acgtttcatt tgacctaccg aaagagcggc ggttcctaga ctaccgcgtc 6960

NEOKAN_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 gggatcaagc tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 7020

6961 ccctagttcg agactagttc tctgtcctac tcctagcaaa gcgtactaac ttgttctacc 7020

.......... .......... .......... .......... ....... ORF 2 NeoR/KanR

.......... .......... .......... .......... .......... ..........

7021 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 7080

7021 taacgtgcgt ccaagaggcc ggcgaaccca cctctccgat aagccgatac tgacccgtgt 7080

.......... ....... ORF 2 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 7140

7081 tgtctgttag ccgacgagac tacggcggca caaggccgac agtcgcgtcc ccgcgggcca 7140

.......... ....... ORF 2 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg 7200

7141 agaaaaacag ttctggctgg acaggccacg ggacttactt gacgttctgc tccgtcgcgc 7200

.......... ....... ORF 2 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 7260

7201 cgatagcacc gaccggtgct gcccgcaagg aacgcgtcga cacgagctgc aacagtgact 7260

.......... ....... ORF 2 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 7320

7261 tcgcccttcc ctgaccgacg ataacccgct tcacggcccc gtcctagagg acagtagagt 7320

.......... ....... ORF 2 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 7380

7321 ggaacgagga cggctctttc ataggtagta ccgactacgt tacgccgccg acgtatgcga 7380

.......... ....... ORF 2 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 7440

7381 actaggccga tggacgggta agctggtggt tcgctttgta gcgtagctcg ctcgtgcatg 7440

.......... ....... ORF 2 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 7500

7441 agcctacctt cggccagaac agctagtcct actagacctg cttctcgtag tccccgagcg 7500

.......... ....... ORF 2 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt 7560

7501 cggtcggctt gacaagcggt ccgagttccg ctcgtacggg ctgccgctcc tagagcagca 7560

.......... ....... ORF 2 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 7620

7561 ctgggtaccg ctacggacga acggcttata gtaccacctt ttaccggcga aaagacctaa 7620

.......... ....... ORF 2 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 7680

7621 gtagctgaca ccggccgacc cacaccgcct ggcgatagtc ctgtatcgca accgatgggc 7680

.......... ....... ORF 2 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 7740

7681 actataacga cttctcgaac cgccgcttac ccgactggcg aaggagcacg aaatgccata 7740

.......... ....... ORF 2 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat 7800

7741 gcggcgaggg ctaagcgtcg cgtagcggaa gatagcggaa gaactgctca agaagactta 7800

.......... ....... ORF 2 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 tttgttaaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaca 7860

7801 aaacaatttt aaaaacaatt tagtcgagta aaaaattggt tatccggctt tagccgttgt 7860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 tcccttataa atcaaaagaa tagaccgcga tagggttgag tgttgttcca gtttggaaca 7920

7861 agggaatatt tagttttctt atctggcgct atcccaactc acaacaaggt caaaccttgt 7920

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

7921 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 7980

7921 tctcaggtga taatttcttg cacctgaggt tgcagtttcc cgctttttgg cagatagtcc 7980

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttgcggtcg aggtgccgta 8040

7981 cgctaccggg tgatgcactt ggtagtgggt ttagttcaaa aaacgccagc tccacggcat 8040

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 aagctctaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 8100

8041 ttcgagattt agccttggga tttccctcgg gggctaaatc tcgaactgcc cctttcggcc 8100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 8160

8101 gcttgcaccg ctctttcctt cccttctttc gctttcctcg cccgcgatcc cgcgaccgtt 8160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 gtgtagcggt cacgctgcgc gtaaccacca cacccgcgcg cttaatgcgc cg........ 8212

8161 cacatcgcca gtgcgacgcg cattggtggt gtgggcgcgc gaattacgcg gc........ 8212

.......... .......... f1_origin .......... .......... ..........