Sequence of pShuttle-CMV-EGFP-C

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Sequence of pShuttle-CMV-EGFP-C

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Sequence of pShuttle-CMV-EGFP-C : 8156 BP; 2006 A; 2122 C; 2175 G; 1831 T; 22 other;

.......... .......... .......... .......... .......... ..........

1 nntcccttcc agctctctgc cccttttgga ttgaagccaa tatgataatg agggggtgga 60

1 nnagggaagg tcgagagacg gggaaaacct aacttcggtt atactattac tcccccacct 60

.......... .......... .......... .......... RITR LITR ..........

.......... .......... .......... .......... .......... ..........

61 gtttgtgacg tggcgcgggg cgtgggaacg gggcgggtga cgtagtagtg tggcggaagt 120

61 caaacactgc accgcgcccc gcacccttgc cccgcccact gcatcatcac accgccttca 120

.......... .......... RITR LITR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gtgatgttgc aagtgtggcg gaacacatgt aagcgacgga tgtggcaaaa gtgacgtttt 180

121 cactacaacg ttcacaccgc cttgtgtaca ttcgctgcct acaccgtttt cactgcaaaa 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tggtgtgcgc cggtgtacac aggaagtgac aattttcgcg cggttttagg cggatgttgt 240

181 accacacgcg gccacatgtg tccttcactg ttaaaagcgc gccaaaatcc gcctacaaca 240

.......... .......... .......... Encap .......... ..........

.......... .......... .......... .......... .......... ..........

241 agtaaatttg ggcgtaaccg agtaagattt ggccattttc gcgggaaaac tgaataagag 300

241 tcatttaaac ccgcattggc tcattctaaa ccggtaaaag cgcccttttg acttattctc 300

.......... .......... Encap .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gaagtgaaat ctgaataatt ttgtgttact catagcgcgt aannnntaat agtaatcaat 360

301 cttcacttta gacttattaa aacacaatga gtatcgcgca ttnnnnatta tcattagtta 360

.......... Encap .......... .......... ......... CMV_imm_pro

.......... .......... .......... .......... .......... ..........

361 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 420

361 atgccccagt aatcaagtat cgggtatata cctcaaggcg caatgtattg aatgccattt 420

.......... ......... CMV_imm_pro ......... .......... CAG_enh

.......... .......... .......... .......... .......... ..........

421 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 480

421 accgggcgga ccgactggcg ggttgctggg ggcgggtaac tgcagttatt actgcataca 480

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 540

481 agggtatcat tgcggttatc cctgaaaggt aactgcagtt acccacctca taaatgccat 540

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... NdeI(573) .......... ..........

541 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 600

541 ttgacgggtg aaccgtcatg tagttcacat agtatacggt tcatgcgggg gataactgca 600

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

601 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 660

601 gttactgcca tttaccgggc ggaccgtaat acgggtcatg tactggaata ccctgaaagg 660

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca 720

661 atgaaccgtc atgtagatgc ataatcagta gcgataatgg taccactacg ccaaaaccgt 720

......... CAG_enh CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat 780

721 catgtagtta cccgcaccta tcgccaaact gagtgcccct aaaggttcag aggtggggta 780

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa 840

781 actgcagtta ccctcaaaca aaaccgtggt tttagttgcc ctgaaaggtt ttacagcatt 840

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag 900

841 gttgaggcgg ggtaactgcg tttacccgcc atccgcacat gccaccctcc agatatattc 900

.......... ... CMV_imm_pro CMV_fwd_pri ... .......... CMV_pro

.......... .......... .......... ClaI(938) .......... ..........

901 cagagctggt ttagtgaacc gtcagatccg ctagagatcg atctggtacc gtcgacgtcg 960

901 gtctcgacca aatcacttgg cagtctaggc gatctctagc tagaccatgg cagctgcagc 960

.......... CMV_pro .......... .......... .......... ..........

NotI(961) .......... .......... .......... .......... ..........

961 cggccgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 1020

961 gccggcggtg gtaccactcg ttcccgctcc tcgacaagtg gccccaccac gggtaggacc 1020

.......... .......... .......... EGFP ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 1080

1021 agctcgacct gccgctgcat ttgccggtgt tcaagtcgca caggccgctc ccgctcccgc 1080

EGFP_N_pri .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 1140

1081 tacggtggat gccgttcgac tgggacttca agtagacgtg gtggccgttc gacgggcacg 1140

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg 1200

1141 ggaccgggtg ggagcactgg tgggactgga tgccgcacgt cacgaagtcg gcgatggggc 1200

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 1260

1201 tggtgtactt cgtcgtgctg aagaagttca ggcggtacgg gcttccgatg caggtcctcg 1260

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 1320

1261 cgtggtagaa gaagttcctg ctgccgttga tgttctgggc gcggctccac ttcaagctcc 1320

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 1380

1321 cgctgtggga ccacttggcg tagctcgact tcccgtagct gaagttcctc ctgccgttgt 1380

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 1440

1381 aggaccccgt gttcgacctc atgttgatgt tgtcggtgtt gcagatatag taccggctgt 1440

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 1500

1441 tcgtcttctt gccgtagttc cacttgaagt tctaggcggt gttgtagctc ctgccgtcgc 1500

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 1560

1501 acgtcgagcg gctggtgatg gtcgtcttgt gggggtagcc gctgccgggg cacgacgacg 1560

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 1620

1561 ggctgttggt gatggactcg tgggtcaggc gggactcgtt tctggggttg ctcttcgcgc 1620

.......... .......... EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 1680

1621 tagtgtacca ggacgacctc aagcactggc ggcggcccta gtgagagccg tacctgctcg 1680

.......... EGFP_C_pri EGFP ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgtacaagtc cggactcaga tctgtaggtg cattaatgga agtgatgcag aagaggagca 1740

1681 acatgttcag gcctgagtct agacatccac gtaattacct tcactacgtc ttctcctcgt 1740

.......... .......... ORF 3 .......... .......... ..........

.......... ....... HindIII(1763) EcoRV(1779) ....... ..........

1741 aagccattca ttcttcagac tgaagcttct agataagata tccgatccac cggatctaga 1800

1741 ttcggtaagt aagaagtctg acttcgaaga tctattctat aggctaggtg gcctagatct 1800

.......... ORF 3 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 1860

1801 attgactagt attagtcggt atggtgtaaa catctccaaa atgaacgaaa ttttttggag 1860

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... HpaI(1911)

1861 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 1920

1861 ggtgtggagg gggacttgga ctttgtattt tacttacgtt aacaacaaca attgaacaaa 1920

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 1980

1921 taacgtcgaa tattaccaat gtttatttcg ttatcgtagt gtttaaagtg tttatttcgt 1980

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgga 2040

1981 aaaaaaagtg acgtaagatc aacaccaaac aggtttgagt agttacatag aattgcgcct 2040

.......... ........ SV40_PA_term EBV_rev_pri ........ ..........

.......... .......... .......... .......... .......... ..........

2041 tctgggcgtg gttaagggtg ggaaagaata tataaggtgg gggtcttatg tagttttgta 2100

2041 agacccgcac caattcccac cctttcttat atattccacc cccagaatac atcaaaacat 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tctgttttgc agcagccgcc gccgccatga gcaccaactc gtttgatgga agcattgtga 2160

2101 agacaaaacg tcgtcggcgg cggcggtact cgtggttgag caaactacct tcgtaacact 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gctcatattt gacaacgcgc atgcccccat gggccggggt gcgtcagaat gtgatgggct 2220

2161 cgagtataaa ctgttgcgcg tacgggggta cccggcccca cgcagtctta cactacccga 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ccagcattga tggtcgcccc gtcctgcccg caaactctac taccttgacc tacgagaccg 2280

2221 ggtcgtaact accagcgggg caggacgggc gtttgagatg atggaactgg atgctctggc 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tgtctggaac gccgttggag actgcagcct ccgccgccgc ttcagccgct gcagccaccg 2340

2281 acagaccttg cggcaacctc tgacgtcgga ggcggcggcg aagtcggcga cgtcggtggc 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cccgcgggat tgtgactgac tttgctttcc tgagcccgct tgcaagcagt gcagcttccc 2400

2341 gggcgcccta acactgactg aaacgaaagg actcgggcga acgttcgtca cgtcgaaggg 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gttcatccgc ccgcgatgac aagttgacgg ctcttttggc acaattggat tctttgaccc 2460

2401 caagtaggcg ggcgctactg ttcaactgcc gagaaaaccg tgttaaccta agaaactggg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gggaacttaa tgtcgtttct cagcagctgt tggatctgcg ccagcaggtt tctgccctga 2520

2461 cccttgaatt acagcaaaga gtcgtcgaca acctagacgc ggtcgtccaa agacgggact 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 aggcttcctc ccctcccaat gcggtttaaa acataaataa aaaaccagac tctgtttgga 2580

2521 tccgaaggag gggagggtta cgccaaattt tgtatttatt ttttggtctg agacaaacct 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tttggatcaa gcaagtgtct tgctgtcttt atttaggggt tttgcgcgcg cggtaggccc 2640

2581 aaacctagtt cgttcacaga acgacagaaa taaatcccca aaacgcgcgc gccatccggg 2640

.......... .......... .......... .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

2641 gggaccagcg gtctcggtcg ttgagggtcc tgtgtatttt ttccaggacg tggtaaaggt 2700

2641 ccctggtcgc cagagccagc aactcccagg acacataaaa aaggtcctgc accatttcca 2700

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gactctggat gttcagatac atgggcataa gcccgtctct ggggtggagg tagcaccact 2760

2701 ctgagaccta caagtctatg tacccgtatt cgggcagaga ccccacctcc atcgtggtga 2760

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcagagcttc atgctgcggg gtggtgttgt agatgatcca gtcgtagcag gagcgctggg 2820

2761 cgtctcgaag tacgacgccc caccacaaca tctactaggt cagcatcgtc ctcgcgaccc 2820

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cgtggtgcct aaaaatgtct ttcagtagca agctgattgc caggggcagg cccttggtgt 2880

2821 gcaccacgga tttttacaga aagtcatcgt tcgactaacg gtccccgtcc gggaaccaca 2880

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aagtgtttac aaagcggtta agctgggatg ggtgcatacg tggggatatg agatgcatct 2940

2881 ttcacaaatg tttcgccaat tcgaccctac ccacgtatgc acccctatac tctacgtaga 2940

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tggactgtat ttttaggttg gctatgttcc cagccatatc cctccgggga ttcatgttgt 3000

2941 acctgacata aaaatccaac cgatacaagg gtcggtatag ggaggcccct aagtacaaca 3000

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gcagaaccac cagcacagtg tatccggtgc acttgggaaa tttgtcatgt agcttagaag 3060

3001 cgtcttggtg gtcgtgtcac ataggccacg tgaacccttt aaacagtaca tcgaatcttc 3060

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gaaatgcgtg gaagaacttg gagacgccct tgtgacctcc aagattttcc atgcattcgt 3120

3061 ctttacgcac cttcttgaac ctctgcggga acactggagg ttctaaaagg tacgtaagca 3120

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccataatgat ggcaatgggc ccacgggcgg cggcctgggc gaagatattt ctgggatcac 3180

3121 ggtattacta ccgttacccg ggtgcccgcc gccggacccg cttctataaa gaccctagtg 3180

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 taacgtcata gttgtgttcc aggatgagat cgtcataggc catttttaca aagcgcgggc 3240

3181 attgcagtat caacacaagg tcctactcta gcagtatccg gtaaaaatgt ttcgcgcccg 3240

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ggagggtgcc agactgcggt ataatggttc catccggccc aggggcgtag ttaccctcac 3300

3241 cctcccacgg tctgacgcca tattaccaag gtaggccggg tccccgcatc aatgggagtg 3300

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 agatttgcat ttcccacgct ttgagttcag atggggggat catgtctacc tgcggggcga 3360

3301 tctaaacgta aagggtgcga aactcaagtc taccccccta gtacagatgg acgccccgct 3360

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tgaagaaaac ggtttccggg gtaggggaga tcagctggga agaaagcagg ttcctgagca 3420

3361 acttcttttg ccaaaggccc catcccctct agtcgaccct tctttcgtcc aaggactcgt 3420

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gctgcgactt accgcagccg gtgggcccgt aaatcacacc tattaccggg tgcaactggt 3480

3421 cgacgctgaa tggcgtcggc cacccgggca tttagtgtgg ataatggccc acgttgacca 3480

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 agttaagaga gctgcagctg ccgtcatccc tgagcagggg ggccacttcg ttaagcatgt 3540

3481 tcaattctct cgacgtcgac ggcagtaggg actcgtcccc ccggtgaagc aattcgtaca 3540

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccctgactcg catgttttcc ctgaccaaat ccgccagaag gcgctcgccg cccagcgata 3600

3541 gggactgagc gtacaaaagg gactggttta ggcggtcttc cgcgagcggc gggtcgctat 3600

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gcagttcttg caaggaagca aagtttttca acggtttgag accgtccgcc gtaggcatgc 3660

3601 cgtcaagaac gttccttcgt ttcaaaaagt tgccaaactc tggcaggcgg catccgtacg 3660

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttttgagcgt ttgaccaagc agttccaggc ggtcccacag ctcggtcacc tgctctacgg 3720

3661 aaaactcgca aactggttcg tcaaggtccg ccagggtgtc gagccagtgg acgagatgcc 3720

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 catctcgatc cagcatatct cctcgtttcg cgggttgggg cggctttcgc tgtacggcag 3780

3721 gtagagctag gtcgtataga ggagcaaagc gcccaacccc gccgaaagcg acatgccgtc 3780

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tagtcggtgc tcgtccagac gggccagggt catgtctttc cacgggcgca gggtcctcgt 3840

3781 atcagccacg agcaggtctg cccggtccca gtacagaaag gtgcccgcgt cccaggagca 3840

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cagcgtagtc tgggtcacgg tgaaggggtg cgctccgggc tgcgcgctgg ccagggtgcg 3900

3841 gtcgcatcag acccagtgcc acttccccac gcgaggcccg acgcgcgacc ggtcccacgc 3900

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cttgaggctg gtcctgctgg tgctgaagcg ctgccggtct tcgccctgcg cgtcggccag 3960

3901 gaactccgac caggacgacc acgacttcgc gacggccaga agcgggacgc gcagccggtc 3960

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gtagcatttg accatggtgt catagtccag cccctccgcg gcgtggccct tggcgcgcag 4020

3961 catcgtaaac tggtaccaca gtatcaggtc ggggaggcgc cgcaccggga accgcgcgtc 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cttgcccttg gaggaggcgc cgcacgaggg gcagtgcaga cttttgaggg cgtagagctt 4080

4021 gaacgggaac ctcctccgcg gcgtgctccc cgtcacgtct gaaaactccc gcatctcgaa 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gggcgcgaga aataccgatt ccggggagta ggcatccgcg ccgcaggccc cgcagacggt 4140

4081 cccgcgctct ttatggctaa ggcccctcat ccgtaggcgc ggcgtccggg gcgtctgcca 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ctcgcattcc acgagccagg tgagctctgg ccgttcgggg tcaaaaacca ggtttccccc 4200

4141 gagcgtaagg tgctcggtcc actcgagacc ggcaagcccc agtttttggt ccaaaggggg 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 atgctttttg atgcgtttct tacctctggt ttccatgagc cggtgtccac gctcggtgac 4260

4201 tacgaaaaac tacgcaaaga atggagacca aaggtactcg gccacaggtg cgagccactg 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... EcoRI(4304) .........

4261 gaaaaggctg tccgtgtccc cgtatacaga ctnnngttta aacgaattcn nnatataaaa 4320

4261 cttttccgac aggcacaggg gcatatgtct gannncaaat ttgcttaagn nntatatttt 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tgcaaggtgc tgctcaaaaa atcaggcaaa gcctcgcgca aaaaagaaag cacatcgtag 4380

4321 acgttccacg acgagttttt tagtccgttt cggagcgcgt tttttctttc gtgtagcatc 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tcatgctcat gcagataaag gcaggtaagc tccggaacca ccacagaaaa agacaccatt 4440

4381 agtacgagta cgtctatttc cgtccattcg aggccttggt ggtgtctttt tctgtggtaa 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tttctctcaa acatgtctgc gggtttctgc ataaacacaa aataaaataa caaaaaaaca 4500

4441 aaagagagtt tgtacagacg cccaaagacg tatttgtgtt ttattttatt gtttttttgt 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tttaaacatt agaagcctgt cttacaacag gaaaaacaac ccttataagc ataagacgga 4560

4501 aaatttgtaa tcttcggaca gaatgttgtc ctttttgttg ggaatattcg tattctgcct 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ctacggccat gccggcgtga ccgtaaaaaa actggtcacc gtgattaaaa agcaccaccg 4620

4561 gatgccggta cggccgcact ggcatttttt tgaccagtgg cactaatttt tcgtggtggc 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 acagctcctc ggtcatgtcc ggagtcataa tgtaagactc ggtaaacaca tcaggttgat 4680

4621 tgtcgaggag ccagtacagg cctcagtatt acattctgag ccatttgtgt agtccaacta 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tcatcggtca gtgctaaaaa gcgaccgaaa tagcccgggg gaatacatac ccgcaggcgt 4740

4681 agtagccagt cacgattttt cgctggcttt atcgggcccc cttatgtatg ggcgtccgca 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 agagacaaca ttacagcccc cataggaggt ataacaaaat taataggaga gaaaaacaca 4800

4741 tctctgttgt aatgtcgggg gtatcctcca tattgtttta attatcctct ctttttgtgt 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 taaacacctg aaaaaccctc ctgcctaggc aaaatagcac cctcccgctc cagaacaaca 4860

4801 atttgtggac tttttgggag gacggatccg ttttatcgtg ggagggcgag gtcttgttgt 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tacagcgctt cacagcggca gcctaacagt cagccttacc agtaaaaaag aaaacctatt 4920

4861 atgtcgcgaa gtgtcgccgt cggattgtca gtcggaatgg tcattttttc ttttggataa 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aaaaaaacac cactcgacac ggcaccagct caatcagtca cagtgtaaaa aagggccaag 4980

4921 tttttttgtg gtgagctgtg ccgtggtcga gttagtcagt gtcacatttt ttcccggttc 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tgcagagcga gtatatatag gactaaaaaa tgacgtaacg gttaaagtcc acaaaaaaca 5040

4981 acgtctcgct catatatatc ctgatttttt actgcattgc caatttcagg tgttttttgt 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cccagaaaac cgcacgcgaa cctacgccca gaaacgaaag ccaaaaaacc cacaacttcc 5100

5041 gggtcttttg gcgtgcgctt ggatgcgggt ctttgctttc ggttttttgg gtgttgaagg 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tcaaatcgtc acttccgttt tcccacgtta cgtaacttcc cattttaaga aaactacaat 5160

5101 agtttagcag tgaaggcaaa agggtgcaat gcattgaagg gtaaaattct tttgatgtta 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tcccaacaca tacaagttac tccgccctaa aacctacgtc acccgccccg ttcccacgcc 5220

5161 agggttgtgt atgttcaatg aggcgggatt ttggatgcag tgggcggggc aagggtgcgg 5220

.......... .......... .......... .......... LITR ..........

.......... .......... .......... .......... .......... ..........

5221 ccgcgccacg tcacaaactc caccccctca ttatcatatt ggcttcaatc caaaataagg 5280

5221 ggcgcggtgc agtgtttgag gtgggggagt aatagtataa ccgaagttag gttttattcc 5280

.......... LITR .......... RITR .......... ..........

.......... ......... BamHI(5307) ......... .......... ..........

5281 tatattattg atgatnnntt aattaaggat ccnnncggtg tgaaataccg cacagatgcg 5340

5281 atataataac tactannnaa ttaattccta ggnnngccac actttatggc gtgtctacgc 5340

RITR .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct 5400

5341 attcctcttt tatggcgtag tccgcgagaa ggcgaaggag cgagtgactg agcgacgcga 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 5460

5401 gccagcaagc cgacgccgct cgccatagtc gagtgagttt ccgccattat gccaataggt 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 5520

5461 gtcttagtcc cctattgcgt cctttcttgt acactcgttt tccggtcgtt ttccggtcct 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 5580

5521 tggcattttt ccggcgcaac gaccgcaaaa aggtatccga ggcgggggga ctgctcgtag 5580

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

5581 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 5640

5581 tgtttttagc tgcgagttca gtctccaccg ctttgggctg tcctgatatt tctatggtcc 5640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5641 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 5700

5641 gcaaaggggg accttcgagg gagcacgcga gaggacaagg ctgggacggc gaatggccta 5700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5701 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 5760

5701 tggacaggcg gaaagaggga agcccttcgc accgcgaaag agtatcgagt gcgacatcca 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 5820

5761 tagagtcaag ccacatccag caagcgaggt tcgacccgac acacgtgctt ggggggcaag 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 5880

5821 tcgggctggc gacgcggaat aggccattga tagcagaact caggttgggc cattctgtgc 5880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5881 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 5940

5881 tgaatagcgg tgaccgtcgt cggtgaccat tgtcctaatc gtctcgctcc atacatccgc 5940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 6000

5941 cacgatgtct caagaacttc accaccggat tgatgccgat gtgatcttcc tgtcataaac 6000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 6060

6001 catagacgcg agacgacttc ggtcaatgga agcctttttc tcaaccatcg agaactaggc 6060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6061 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 6120

6061 cgtttgtttg gtggcgacca tcgccaccaa aaaaacaaac gttcgtcgtc taatgcgcgt 6120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 6180

6121 ctttttttcc tagagttctt ctaggaaact agaaaagatg ccccagactg cgagtcacct 6180

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 6240

6181 tgcttttgag tgcaattccc taaaaccagt actctaatag tttttcctag aagtggatct 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 6300

6241 aggaaaattt aatttttact tcaaaattta gttagatttc atatatactc atttgaacca 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 6360

6301 gactgtcaat ggttacgaat tagtcactcc gtggatagag tcgctagaca gataaagcaa 6360

.......... .......... ...... Ampicillin(300-0) ...... ..........

.......... .......... .......... .......... .......... ..........

6361 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 6420

6361 gtaggtatca acggactgag gggcagcaca tctattgatg ctatgccctc ccgaatggta 6420

.......... ...... Ampicillin(300-0) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 6480

6421 gaccggggtc acgacgttac tatggcgctc tgggtgcgag tggccgaggt ctaaatagtc 6480

.......... ...... Ampicillin(300-0) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 6540

6481 gttatttggt cggtcggcct tcccggctcg cgtcttcacc aggacgttga aataggcgga 6540

.......... ...... Ampicillin(300-0) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 6600

6541 ggtaggtcag ataattaaca acggcccttc gatctcattc atcaagcggt caattatcaa 6600

.......... ...... Ampicillin(300-0) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 tgcgcaacgt tgttgnnnna aaaaggatct tcacctagat ccttttcacg tagaaagcca 6660

6601 acgcgttgca acaacnnnnt ttttcctaga agtggatcta ggaaaagtgc atctttcggt 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg 6720

6661 caggcgtctt tgccacgact ggggcctact tacagtcgat gacccgatag acctgttccc 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag 6780

6721 ttttgcgttc gcgtttctct ttcgtccatc gaacgtcacc cgaatgtacc gctatcgatc 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta 6840

6781 tgacccgcca aaatacctgt cgttcgcttg gccttaacgg tcgaccccgc gggagaccat 6840

.......... .......... .......... .......... NEOKAN_pro ..........

.......... .......... .......... .......... .......... ..........

6841 aggttgggaa gccctgcaaa gtaaactgga tggctttctc gccgccaagg atctgatggc 6900

6841 tccaaccctt cgggacgttt catttgacct accgaaagag cggcggttcc tagactaccg 6900

NEOKAN_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag 6960

6901 cgtcccctag ttcgagacta gttctctgtc ctactcctag caaagcgtac taacttgttc 6960

.......... .......... .......... .......... ....... ORF 3 NeoR/KanR

.......... .......... .......... .......... .......... ..........

6961 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 7020

6961 tacctaacgt gcgtccaaga ggccggcgaa cccacctctc cgataagccg atactgaccc 7020

.......... ....... ORF 3 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 7080

7021 gtgttgtctg ttagccgacg agactacggc ggcacaaggc cgacagtcgc gtccccgcgg 7080

.......... ....... ORF 3 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag 7140

7081 gccaagaaaa acagttctgg ctggacaggc cacgggactt acttgacgtt ctgctccgtc 7140

.......... ....... ORF 3 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 7200

7141 gcgccgatag caccgaccgg tgctgcccgc aaggaacgcg tcgacacgag ctgcaacagt 7200

.......... ....... ORF 3 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 7260

7201 gacttcgccc ttccctgacc gacgataacc cgcttcacgg ccccgtccta gaggacagta 7260

.......... ....... ORF 3 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 7320

7261 gagtggaacg aggacggctc tttcataggt agtaccgact acgttacgcc gccgacgtat 7320

.......... ....... ORF 3 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 7380

7321 gcgaactagg ccgatggacg ggtaagctgg tggttcgctt tgtagcgtag ctcgctcgtg 7380

.......... ....... ORF 3 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 7440

7381 catgagccta ccttcggcca gaacagctag tcctactaga cctgcttctc gtagtccccg 7440

.......... ....... ORF 3 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg 7500

7441 agcgcggtcg gcttgacaag cggtccgagt tccgctcgta cgggctgccg ctcctagagc 7500

.......... ....... ORF 3 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 7560

7501 agcactgggt accgctacgg acgaacggct tatagtacca ccttttaccg gcgaaaagac 7560

.......... ....... ORF 3 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 7620

7561 ctaagtagct gacaccggcc gacccacacc gcctggcgat agtcctgtat cgcaaccgat 7620

.......... ....... ORF 3 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 7680

7621 gggcactata acgacttctc gaaccgccgc ttacccgact ggcgaaggag cacgaaatgc 7680

.......... ....... ORF 3 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 7740

7681 catagcggcg agggctaagc gtcgcgtagc ggaagatagc ggaagaactg ctcaagaaga 7740

.......... ....... ORF 3 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 gaattttgtt aaaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 7800

7741 cttaaaacaa ttttaaaaac aatttagtcg agtaaaaaat tggttatccg gctttagccg 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 aacatccctt ataaatcaaa agaatagacc gcgatagggt tgagtgttgt tccagtttgg 7860

7801 ttgtagggaa tatttagttt tcttatctgg cgctatccca actcacaaca aggtcaaacc 7860

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

7861 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 7920

7861 ttgttctcag gtgataattt cttgcacctg aggttgcagt ttcccgcttt ttggcagata 7920

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttgcg gtcgaggtgc 7980

7921 gtcccgctac cgggtgatgc acttggtagt gggtttagtt caaaaaacgc cagctccacg 7980

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 cgtaaagctc taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 8040

7981 gcatttcgag atttagcctt gggatttccc tcgggggcta aatctcgaac tgcccctttc 8040

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg 8100

8041 ggccgcttgc accgctcttt ccttcccttc tttcgctttc ctcgcccgcg atcccgcgac 8100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg cgcgcttaat gcgccg.... 8156

8101 cgttcacatc gccagtgcga cgcgcattgg tggtgtgggc gcgcgaatta cgcggc.... 8156

.......... .......... f1_origin .......... .......... ..........