GenBank/Entrez ID of insert: AF334399
Vector backbone: pShuttle
Type of vector: Mammalian expression,Adenoviral
Backbone size (bp): 6736
Bacteria resistance: Kanamycin
High or low copy: Low Copy
Grow in standard E. coli @ 37C: Yes
Comments: Vector pShuttle is used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired. Max insert size = 7.5kb with pAdEasy1 and 10.2kb with pAdEasy2.
Article: A simplified system for generating recombinant adenoviruses. He TC et al. (Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. Pubmed)