GenBank/Entrez ID of insert: AF334399
Vector backbone: pShuttle-CMV
Type of vector: Mammalian expression,Adenoviral
Backbone size (bp): 7452
5' Sequencing primer: CMV-F (List of Sequencing Primers)
Bacteria resistance: Kanamycin
High or low copy: Low Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH10B
Principal Investigator: Bert Vogelstein
Comments: Vector pShuttle-CMV is used for expression of transgenes under a CMV promoter when no GFP tracer is desired. Max insert size = 6.6kb with pAdEasy1 and 9.3kb with pAdEasy2.
Article: A simplified system for generating recombinant adenoviruses. He TC et al. (Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. Pubmed)