Sequence of pShuttle

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Sequence of pShuttle_cmv

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Sequence of pShuttle-CMV : 7384 BP; 1814 A; 1864 C; 1944 G; 1711 T; 51 other;

.......... .......... .......... .......... .......... ..........

1 nnnttaatta annntccctt ccagctctct gccccttttg gattgaagcc aatatgataa 60

1 nnnaattaat tnnnagggaa ggtcgagaga cggggaaaac ctaacttcgg ttatactatt 60

.......... .......... .......... .......... RITR LITR ..........

.......... .......... .......... .......... .......... ..........

61 tgagggggtg gagtttgtga cgtggcgcgg ggcgtgggaa cggggcgggt gacgtagtag 120

61 actcccccac ctcaaacact gcaccgcgcc ccgcaccctt gccccgccca ctgcatcatc 120

.......... RITR LITR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tgtggcggaa gtgtgatgtt gcaagtgtgg cggaacacat gtaagcgacg gatgtggcaa 180

121 acaccgcctt cacactacaa cgttcacacc gccttgtgta cattcgctgc ctacaccgtt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aagtgacgtt tttggtgtgc gccggtgtac acaggaagtg acaattttcg cgcggtttta 240

181 ttcactgcaa aaaccacacg cggccacatg tgtccttcac tgttaaaagc gcgccaaaat 240

.......... .......... .......... Encap .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggcggatgtt gtagtaaatt tgggcgtaac cgagtaagat ttggccattt tcgcgggaaa 300

241 ccgcctacaa catcatttaa acccgcattg gctcattcta aaccggtaaa agcgcccttt 300

.......... .......... Encap .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 actgaataag aggaagtgaa atctgaataa ttttgtgtta ctcatagcgc gtaannnnta 360

301 tgacttattc tccttcactt tagacttatt aaaacacaat gagtatcgcg cattnnnnat 360

.......... .......... Encap .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 420

361 tatcattagt taatgcccca gtaatcaagt atcgggtata tacctcaagg cgcaatgtat 420

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 480

421 tgaatgccat ttaccgggcg gaccgactgg cgggttgctg ggggcgggta actgcagtta 480

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

481 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 540

481 ttactgcata caagggtatc attgcggtta tccctgaaag gtaactgcag ttacccacct 540

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... NdeI(585) ..........

541 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 600

541 cataaatgcc atttgacggg tgaaccgtca tgtagttcac atagtatacg gttcatgcgg 600

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 660

601 gggataactg cagttactgc catttaccgg gcggaccgta atacgggtca tgtactggaa 660

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

661 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 720

661 taccctgaaa ggatgaaccg tcatgtagat gcataatcag tagcgataat ggtaccacta 720

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 780

721 cgccaaaacc gtcatgtagt tacccgcacc tatcgccaaa ctgagtgccc ctaaaggttc 780

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 840

781 agaggtgggg taactgcagt taccctcaaa caaaaccgtg gttttagttg ccctgaaagg 840

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 900

841 ttttacagca ttgttgaggc ggggtaactg cgtttacccg ccatccgcac atgccaccct 900

.......... ......... CMV_imm_pro CMV_f_pri ......... CMV_pro

.......... .......... .......... .......... BglII(947) ..........

901 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagagat ctggtaccgt 960

901 ccagatatat tcgtctcgac caaatcactt ggcagtctag gcgatctcta gaccatggca 960

CMV_imm_pro CMV_pro ........ .......... .......... ..........

NotI(966) XhoI(972) HindIII(981) EcoRV(997) ........ ..........

961 cgacgcggcc gctcgagcct aagcttctag ataagatatc cgatccaccg gatctagata 1020

961 gctgcgccgg cgagctcgga ttcgaagatc tattctatag gctaggtggc ctagatctat 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc 1080

1021 tgactagtat tagtcggtat ggtgtaaaca tctccaaaat gaacgaaatt ttttggaggg 1080

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... HpaI(1129) ..........

1081 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat 1140

1081 tgtggagggg gacttggact ttgtatttta cttacgttaa caacaacaat tgaacaaata 1140

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 1200

1141 acgtcgaata ttaccaatgt ttatttcgtt atcgtagtgt ttaaagtgtt tatttcgtaa 1200

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcnnnnt 1260

1201 aaaaagtgac gtaagatcaa caccaaacag gtttgagtag ttacatagaa ttgcgnnnna 1260

........ EBV_rev_pri SV40_PA_term ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aagggtggga aagaatatat aaggtggggg tcttatgtag ttttgtatct gttttgcagc 1320

1261 ttcccaccct ttcttatata ttccaccccc agaatacatc aaaacataga caaaacgtcg 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 agccgccgcc gccatgagca ccaactcgtt tgatggaagc attgtgagct catatttgac 1380

1321 tcggcggcgg cggtactcgt ggttgagcaa actaccttcg taacactcga gtataaactg 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aacgcgcatg cccccatggg ccggggtgcg tcagaatgtg atgggctcca gcattgatgg 1440

1381 ttgcgcgtac gggggtaccc ggccccacgc agtcttacac tacccgaggt cgtaactacc 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tcgccccgtc ctgcccgcaa actctactac cttgacctac gagaccgtgt ctggaacgcc 1500

1441 agcggggcag gacgggcgtt tgagatgatg gaactggatg ctctggcaca gaccttgcgg 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gttggagact gcagcctccg ccgccgcttc agccgctgca gccaccgccc gcgggattgt 1560

1501 caacctctga cgtcggaggc ggcggcgaag tcggcgacgt cggtggcggg cgccctaaca 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gactgacttt gctttcctga gcccgcttgc aagcagtgca gcttcccgtt catccgcccg 1620

1561 ctgactgaaa cgaaaggact cgggcgaacg ttcgtcacgt cgaagggcaa gtaggcgggc 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cgatgacaag ttgacggctc ttttggcaca attggattct ttgacccggg aacttaatgt 1680

1621 gctactgttc aactgccgag aaaaccgtgt taacctaaga aactgggccc ttgaattaca 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cgtttctcag cagctgttgg atctgcgcca gcaggtttct gccctgaagg cttcctcccc 1740

1681 gcaaagagtc gtcgacaacc tagacgcggt cgtccaaaga cgggacttcc gaaggagggg 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tcccaatgcg gtttaaaaca taaataaaaa accagactct gtttggattt ggatcaagca 1800

1741 agggttacgc caaattttgt atttattttt tggtctgaga caaacctaaa cctagttcgt 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg taggcccggg accagcggtc 1860

1801 tcacagaacg acagaaataa atccccaaaa cgcgcgcgcc atccgggccc tggtcgccag 1860

.......... .......... .......... ORF1 .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tcggtcgttg agggtcctgt gtattttttc caggacgtgg taaaggtgac tctggatgtt 1920

1861 agccagcaac tcccaggaca cataaaaaag gtcctgcacc atttccactg agacctacaa 1920

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cagatacatg ggcataagcc cgtctctggg gtggaggtag caccactgca gagcttcatg 1980

1921 gtctatgtac ccgtattcgg gcagagaccc cacctccatc gtggtgacgt ctcgaagtac 1980

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag cgctgggcgt ggtgcctaaa 2040

1981 gacgccccac cacaacatct actaggtcag catcgtcctc gcgacccgca ccacggattt 2040

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 aatgtctttc agtagcaagc tgattgccag gggcaggccc ttggtgtaag tgtttacaaa 2100

2041 ttacagaaag tcatcgttcg actaacggtc cccgtccggg aaccacattc acaaatgttt 2100

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gcggttaagc tgggatgggt gcatacgtgg ggatatgaga tgcatcttgg actgtatttt 2160

2101 cgccaattcg accctaccca cgtatgcacc cctatactct acgtagaacc tgacataaaa 2160

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 taggttggct atgttcccag ccatatccct ccggggattc atgttgtgca gaaccaccag 2220

2161 atccaaccga tacaagggtc ggtataggga ggcccctaag tacaacacgt cttggtggtc 2220

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cacagtgtat ccggtgcact tgggaaattt gtcatgtagc ttagaaggaa atgcgtggaa 2280

2221 gtgtcacata ggccacgtga accctttaaa cagtacatcg aatcttcctt tacgcacctt 2280

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gaacttggag acgcccttgt gacctccaag attttccatg cattcgtcca taatgatggc 2340

2281 cttgaacctc tgcgggaaca ctggaggttc taaaaggtac gtaagcaggt attactaccg 2340

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aatgggccca cgggcggcgg cctgggcgaa gatatttctg ggatcactaa cgtcatagtt 2400

2341 ttacccgggt gcccgccgcc ggacccgctt ctataaagac cctagtgatt gcagtatcaa 2400

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gtgttccagg atgagatcgt cataggccat ttttacaaag cgcgggcgga gggtgccaga 2460

2401 cacaaggtcc tactctagca gtatccggta aaaatgtttc gcgcccgcct cccacggtct 2460

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctgcggtata atggttccat ccggcccagg ggcgtagtta ccctcacaga tttgcatttc 2520

2461 gacgccatat taccaaggta ggccgggtcc ccgcatcaat gggagtgtct aaacgtaaag 2520

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ccacgctttg agttcagatg gggggatcat gtctacctgc ggggcgatga agaaaacggt 2580

2521 ggtgcgaaac tcaagtctac ccccctagta cagatggacg ccccgctact tcttttgcca 2580

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttccggggta ggggagatca gctgggaaga aagcaggttc ctgagcagct gcgacttacc 2640

2581 aaggccccat cccctctagt cgacccttct ttcgtccaag gactcgtcga cgctgaatgg 2640

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcagccggtg ggcccgtaaa tcacacctat taccgggtgc aactggtagt taagagagct 2700

2641 cgtcggccac ccgggcattt agtgtggata atggcccacg ttgaccatca attctctcga 2700

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcagctgccg tcatccctga gcaggggggc cacttcgtta agcatgtccc tgactcgcat 2760

2701 cgtcgacggc agtagggact cgtccccccg gtgaagcaat tcgtacaggg actgagcgta 2760

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gttttccctg accaaatccg ccagaaggcg ctcgccgccc agcgatagca gttcttgcaa 2820

2761 caaaagggac tggtttaggc ggtcttccgc gagcggcggg tcgctatcgt caagaacgtt 2820

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ggaagcaaag tttttcaacg gtttgagacc gtccgccgta ggcatgcttt tgagcgtttg 2880

2821 ccttcgtttc aaaaagttgc caaactctgg caggcggcat ccgtacgaaa actcgcaaac 2880

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 accaagcagt tccaggcggt cccacagctc ggtcacctgc tctacggcat ctcgatccag 2940

2881 tggttcgtca aggtccgcca gggtgtcgag ccagtggacg agatgccgta gagctaggtc 2940

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 catatctcct cgtttcgcgg gttggggcgg ctttcgctgt acggcagtag tcggtgctcg 3000

2941 gtatagagga gcaaagcgcc caaccccgcc gaaagcgaca tgccgtcatc agccacgagc 3000

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tccagacggg ccagggtcat gtctttccac gggcgcaggg tcctcgtcag cgtagtctgg 3060

3001 aggtctgccc ggtcccagta cagaaaggtg cccgcgtccc aggagcagtc gcatcagacc 3060

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gtcacggtga aggggtgcgc tccgggctgc gcgctggcca gggtgcgctt gaggctggtc 3120

3061 cagtgccact tccccacgcg aggcccgacg cgcgaccggt cccacgcgaa ctccgaccag 3120

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt cggccaggta gcatttgacc 3180

3121 gacgaccacg acttcgcgac ggccagaagc gggacgcgca gccggtccat cgtaaactgg 3180

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atggtgtcat agtccagccc ctccgcggcg tggcccttgg cgcgcagctt gcccttggag 3240

3181 taccacagta tcaggtcggg gaggcgccgc accgggaacc gcgcgtcgaa cgggaacctc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gaggcgccgc acgaggggca gtgcagactt ttgagggcgt agagcttggg cgcgagaaat 3300

3241 ctccgcggcg tgctccccgt cacgtctgaa aactcccgca tctcgaaccc gcgctcttta 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 accgattccg gggagtaggc atccgcgccg caggccccgc agacggtctc gcattccacg 3360

3301 tggctaaggc ccctcatccg taggcgcggc gtccggggcg tctgccagag cgtaaggtgc 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 agccaggtga gctctggccg ttcggggtca aaaaccaggt ttcccccatg ctttttgatg 3420

3361 tcggtccact cgagaccggc aagccccagt ttttggtcca aagggggtac gaaaaactac 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cgtttcttac ctctggtttc catgagccgg tgtccacgct cggtgacgaa aaggctgtcc 3480

3421 gcaaagaatg gagaccaaag gtactcggcc acaggtgcga gccactgctt ttccgacagg 3480

.......... .......... .......... .......... .......... ..........

.......... .......... ......... EcoRI(3511) ......... ..........

3481 gtgtccccgt atacagactn nngtttaaac gaattcnnna tataaaatgc aaggtgctgc 3540

3481 cacaggggca tatgtctgan nncaaatttg cttaagnnnt atattttacg ttccacgacg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tcaaaaaatc aggcaaagcc tcgcgcaaaa aagaaagcac atcgtagtca tgctcatgca 3600

3541 agttttttag tccgtttcgg agcgcgtttt ttctttcgtg tagcatcagt acgagtacgt 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gataaaggca ggtaagctcc ggaaccacca cagaaaaaga caccattttt ctctcaaaca 3660

3601 ctatttccgt ccattcgagg ccttggtggt gtctttttct gtggtaaaaa gagagtttgt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tgtctgcggg tttctgcata aacacaaaat aaaataacaa aaaaacattt aaacattaga 3720

3661 acagacgccc aaagacgtat ttgtgtttta ttttattgtt tttttgtaaa tttgtaatct 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agcctgtctt acaacaggaa aaacaaccct tataagcata agacggacta cggccatgcc 3780

3721 tcggacagaa tgttgtcctt tttgttggga atattcgtat tctgcctgat gccggtacgg 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggcgtgaccg taaaaaaact ggtcaccgtg attaaaaagc accaccgaca gctcctcggt 3840

3781 ccgcactggc atttttttga ccagtggcac taatttttcg tggtggctgt cgaggagcca 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 catgtccgga gtcataatgt aagactcggt aaacacatca ggttgattca tcggtcagtg 3900

3841 gtacaggcct cagtattaca ttctgagcca tttgtgtagt ccaactaagt agccagtcac 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ctaaaaagcg accgaaatag cccgggggaa tacatacccg caggcgtaga gacaacatta 3960

3901 gatttttcgc tggctttatc gggccccctt atgtatgggc gtccgcatct ctgttgtaat 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cagcccccat aggaggtata acaaaattaa taggagagaa aaacacataa acacctgaaa 4020

3961 gtcgggggta tcctccatat tgttttaatt atcctctctt tttgtgtatt tgtggacttt 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aaccctcctg cctaggcaaa atagcaccct cccgctccag aacaacatac agcgcttcac 4080

4021 ttgggaggac ggatccgttt tatcgtggga gggcgaggtc ttgttgtatg tcgcgaagtg 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 agcggcagcc taacagtcag ccttaccagt aaaaaagaaa acctattaaa aaaacaccac 4140

4081 tcgccgtcgg attgtcagtc ggaatggtca ttttttcttt tggataattt ttttgtggtg 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tcgacacggc accagctcaa tcagtcacag tgtaaaaaag ggccaagtgc agagcgagta 4200

4141 agctgtgccg tggtcgagtt agtcagtgtc acattttttc ccggttcacg tctcgctcat 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tatataggac taaaaaatga cgtaacggtt aaagtccaca aaaaacaccc agaaaaccgc 4260

4201 atatatcctg attttttact gcattgccaa tttcaggtgt tttttgtggg tcttttggcg 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 acgcgaacct acgcccagaa acgaaagcca aaaaacccac aacttcctca aatcgtcact 4320

4261 tgcgcttgga tgcgggtctt tgctttcggt tttttgggtg ttgaaggagt ttagcagtga 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tccgttttcc cacgttacgt aacttcccat tttaagaaaa ctacaattcc caacacatac 4380

4321 aggcaaaagg gtgcaatgca ttgaagggta aaattctttt gatgttaagg gttgtgtatg 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aagttactcc gccctaaaac ctacgtcacc cgccccgttc ccacgccccg cgccacgtca 4440

4381 ttcaatgagg cgggattttg gatgcagtgg gcggggcaag ggtgcggggc gcggtgcagt 4440

.......... .......... .......... LITR RITR .......... ..........

.......... .......... .......... .......... .......... ..........

4441 caaactccac cccctcatta tcatattggc ttcaatccaa aataaggtat attattgatg 4500

4441 gtttgaggtg ggggagtaat agtataaccg aagttaggtt ttattccata taataactac 4500

.......... .......... LITR RITR .......... .......... ..........

......... BamHI(4514) ......... .......... .......... ..........

4501 atnnnttaat taaggatccn nncggtgtga aataccgcac agatgcgtaa ggagaaaata 4560

4501 tannnaatta attcctaggn nngccacact ttatggcgtg tctacgcatt cctcttttat 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 4620

4561 ggcgtagtcc gcgagaaggc gaaggagcga gtgactgagc gacgcgagcc agcaagccga 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 4680

4621 cgccgctcgc catagtcgag tgagtttccg ccattatgcc aataggtgtc ttagtcccct 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 4740

4681 attgcgtcct ttcttgtaca ctcgttttcc ggtcgttttc cggtccttgg catttttccg 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 4800

4741 gcgcaacgac cgcaaaaagg tatccgaggc ggggggactg ctcgtagtgt ttttagctgc 4800

.......... .......... pBR_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 4860

4801 gagttcagtc tccaccgctt tgggctgtcc tgatatttct atggtccgca aagggggacc 4860

.......... .......... pBR_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 4920

4861 ttcgagggag cacgcgagag gacaaggctg ggacggcgaa tggcctatgg acaggcggaa 4920

.......... .......... pBR_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 4980

4921 agagggaagc ccttcgcacc gcgaaagagt atcgagtgcg acatccatag agtcaagcca 4980

.......... .......... pBR_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 5040

4981 catccagcaa gcgaggttcg acccgacaca cgtgcttggg gggcaagtcg ggctggcgac 5040

.......... .......... pBR_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 5100

5041 gcggaatagg ccattgatag cagaactcag gttgggccat tctgtgctga atagcggtga 5100

.......... .......... pBR_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 5160

5101 ccgtcgtcgg tgaccattgt cctaatcgtc tcgctccata catccgccac gatgtctcaa 5160

.......... .......... pBR_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 5220

5161 gaacttcacc accggattga tgccgatgtg atcttcctgt cataaaccat agacgcgaga 5220

.......... .......... pBR_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 5280

5221 cgacttcggt caatggaagc ctttttctca accatcgaga actaggccgt ttgtttggtg 5280

.......... .......... pBR_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 5340

5281 gcgaccatcg ccaccaaaaa aacaaacgtt cgtcgtctaa tgcgcgtctt tttttcctag 5340

.......... .......... pBR_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 5400

5341 agttcttcta ggaaactaga aaagatgccc cagactgcga gtcaccttgc ttttgagtgc 5400

pBR_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 5460

5401 aattccctaa aaccagtact ctaatagttt ttcctagaag tggatctagg aaaatttaat 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 5520

5461 ttttacttca aaatttagtt agatttcata tatactcatt tgaaccagac tgtcaatggt 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 5580

5521 tacgaattag tcactccgtg gatagagtcg ctagacagat aaagcaagta ggtatcaacg 5580

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 5640

5581 gactgagggg cagcacatct attgatgcta tgccctcccg aatggtagac cggggtcacg 5640

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 5700

5641 acgttactat ggcgctctgg gtgcgagtgg ccgaggtcta aatagtcgtt atttggtcgg 5700

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 5760

5701 tcggccttcc cggctcgcgt cttcaccagg acgttgaaat aggcggaggt aggtcagata 5760

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 5820

5761 attaacaacg gcccttcgat ctcattcatc aagcggtcaa ttatcaaacg cgttgcaaca 5820

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tgnnnnaaaa aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg 5880

5821 acnnnntttt tcctagaagt ggatctagga aaagtgcatc tttcggtcag gcgtctttgc 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc 5940

5881 cacgactggg gcctacttac agtcgatgac ccgatagacc tgttcccttt tgcgttcgcg 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt 6000

5941 tttctctttc gtccatcgaa cgtcacccga atgtaccgct atcgatctga cccgccaaaa 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc 6060

6001 tacctgtcgt tcgcttggcc ttaacggtcg accccgcggg agaccattcc aacccttcgg 6060

.......... .......... .......... NEOKAN_pro .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ctgcaaagta aactggatgg ctttctcgcc gccaaggatc tgatggcgca ggggatcaag 6120

6061 gacgtttcat ttgacctacc gaaagagcgg cggttcctag actaccgcgt cccctagttc 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 6180

6121 gagactagtt ctctgtccta ctcctagcaa agcgtactaa cttgttctac ctaacgtgcg 6180

.......... .......... .......... ........ ORF2 Neomycin ........

.......... .......... .......... .......... .......... ..........

6181 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 6240

6181 tccaagaggc cggcgaaccc acctctccga taagccgata ctgacccgtg ttgtctgtta 6240

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6241 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 6300

6241 gccgacgaga ctacggcggc acaaggccga cagtcgcgtc cccgcgggcc aagaaaaaca 6300

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6301 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg 6360

6301 gttctggctg gacaggccac gggacttact tgacgttctg ctccgtcgcg ccgatagcac 6360

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6361 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 6420

6361 cgaccggtgc tgcccgcaag gaacgcgtcg acacgagctg caacagtgac ttcgcccttc 6420

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6421 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 6480

6421 cctgaccgac gataacccgc ttcacggccc cgtcctagag gacagtagag tggaacgagg 6480

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6481 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 6540

6481 acggctcttt cataggtagt accgactacg ttacgccgcc gacgtatgcg aactaggccg 6540

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6541 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 6600

6541 atggacgggt aagctggtgg ttcgctttgt agcgtagctc gctcgtgcat gagcctacct 6600

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6601 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 6660

6601 tcggccagaa cagctagtcc tactagacct gcttctcgta gtccccgagc gcggtcggct 6660

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6661 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg 6720

6661 tgacaagcgg tccgagttcc gctcgtacgg gctgccgctc ctagagcagc actgggtacc 6720

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6721 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 6780

6721 gctacggacg aacggcttat agtaccacct tttaccggcg aaaagaccta agtagctgac 6780

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6781 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 6840

6781 accggccgac ccacaccgcc tggcgatagt cctgtatcgc aaccgatggg cactataacg 6840

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6841 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 6900

6841 acttctcgaa ccgccgctta cccgactggc gaaggagcac gaaatgccat agcggcgagg 6900

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6901 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttttgttaaa 6960

6901 gctaagcgtc gcgtagcgga agatagcgga agaactgctc aagaagactt aaaacaattt 6960

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6961 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaac atcccttata 7020

6961 taaaaacaat ttagtcgagt aaaaaattgg ttatccggct ttagccgttg tagggaatat 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 aatcaaaaga atagaccgcg atagggttga gtgttgttcc agtttggaac aagagtccac 7080

7021 ttagttttct tatctggcgc tatcccaact cacaacaagg tcaaaccttg ttctcaggtg 7080

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

7081 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 7140

7081 ataatttctt gcacctgagg ttgcagtttc ccgctttttg gcagatagtc ccgctaccgg 7140

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 cactacgtga accatcaccc aaatcaagtt ttttgcggtc gaggtgccgt aaagctctaa 7200

7141 gtgatgcact tggtagtggg tttagttcaa aaaacgccag ctccacggca tttcgagatt 7200

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 7260

7201 tagccttggg atttccctcg ggggctaaat ctcgaactgc ccctttcggc cgcttgcacc 7260

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 7320

7261 gctctttcct tcccttcttt cgctttcctc gcccgcgatc ccgcgaccgt tcacatcgcc 7320

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 tcacgctgcg cgtaaccacc acacccgcgc gcttaatgcg ccgnnnnnnn nnnnnnnnnn 7380

7321 agtgcgacgc gcattggtgg tgtgggcgcg cgaattacgc ggcnnnnnnn nnnnnnnnnn 7380

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 nnnn...... .......... .......... .......... .......... .......... 7384

7381 nnnn...... .......... .......... .......... .......... .......... 7384

.......... .......... .......... .......... .......... ..........