Sequence of pShuttle

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

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Sequence of pShuttle

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Sequence of pShuttle : 6528 BP; 1582 A; 1664 C; 1769 G; 1476 T; 37 other;

.......... .......... .......... .......... .......... ..........

1 nnnttaatta annntccctt ccagctctct gccccttttg gattgaagcc aatatgataa 60

1 nnnaattaat tnnnagggaa ggtcgagaga cggggaaaac ctaacttcgg ttatactatt 60

.......... .......... .......... .......... RITR LITR ..........

.......... .......... .......... .......... .......... ..........

61 tgagggggtg gagtttgtga cgtggcgcgg ggcgtgggaa cggggcgggt gacgtagtag 120

61 actcccccac ctcaaacact gcaccgcgcc ccgcaccctt gccccgccca ctgcatcatc 120

.......... .......... RITR LITR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tgtggcggaa gtgtgatgtt gcaagtgtgg cggaacacat gtaagcgacg gatgtggcaa 180

121 acaccgcctt cacactacaa cgttcacacc gccttgtgta cattcgctgc ctacaccgtt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aagtgacgtt tttggtgtgc gccggtgtac acaggaagtg acaattttcg cgcggtttta 240

181 ttcactgcaa aaaccacacg cggccacatg tgtccttcac tgttaaaagc gcgccaaaat 240

.......... .......... .......... Encap .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggcggatgtt gtagtaaatt tgggcgtaac cgagtaagat ttggccattt tcgcgggaaa 300

241 ccgcctacaa catcatttaa acccgcattg gctcattcta aaccggtaaa agcgcccttt 300

.......... .......... Encap .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 actgaataag aggaagtgaa atctgaataa ttttgtgtta ctcatagcgc gtaannnggt 360

301 tgacttattc tccttcactt tagacttatt aaaacacaat gagtatcgcg cattnnncca 360

.......... .......... Encap .......... .......... ..........

KpnI,NotI XhoI XbaI EcoRV HindIII SalI BglII(408)

361 accgcggccg cctcgagtct agagatatcg aattcaagct tgtcgacaga tctnnntaag 420

361 tggcgccggc ggagctcaga tctctatagc ttaagttcga acagctgtct agannnattc 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ggtgggaaag aatatataag gtgggggtct tatgtagttt tgtatctgtt ttgcagcagc 480

421 ccaccctttc ttatatattc cacccccaga atacatcaaa acatagacaa aacgtcgtcg 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cgccgccgcc atgagcacca actcgtttga tggaagcatt gtgagctcat atttgacaac 540

481 gcggcggcgg tactcgtggt tgagcaaact accttcgtaa cactcgagta taaactgttg 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gcgcatgccc ccatgggccg gggtgcgtca gaatgtgatg ggctccagca ttgatggtcg 600

541 cgcgtacggg ggtacccggc cccacgcagt cttacactac ccgaggtcgt aactaccagc 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ccccgtcctg cccgcaaact ctactacctt gacctacgag accgtgtctg gaacgccgtt 660

601 ggggcaggac gggcgtttga gatgatggaa ctggatgctc tggcacagac cttgcggcaa 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ggagactgca gcctccgccg ccgcttcagc cgctgcagcc accgcccgcg ggattgtgac 720

661 cctctgacgt cggaggcggc ggcgaagtcg gcgacgtcgg tggcgggcgc cctaacactg 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgactttgct ttcctgagcc cgcttgcaag cagtgcagct tcccgttcat ccgcccgcga 780

721 actgaaacga aaggactcgg gcgaacgttc gtcacgtcga agggcaagta ggcgggcgct 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgacaagttg acggctcttt tggcacaatt ggattctttg acccgggaac ttaatgtcgt 840

781 actgttcaac tgccgagaaa accgtgttaa cctaagaaac tgggcccttg aattacagca 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttctcagcag ctgttggatc tgcgccagca ggtttctgcc ctgaaggctt cctcccctcc 900

841 aagagtcgtc gacaacctag acgcggtcgt ccaaagacgg gacttccgaa ggaggggagg 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 caatgcggtt taaaacataa ataaaaaacc agactctgtt tggatttgga tcaagcaagt 960

901 gttacgccaa attttgtatt tattttttgg tctgagacaa acctaaacct agttcgttca 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gtcttgctgt ctttatttag gggttttgcg cgcgcggtag gcccgggacc agcggtctcg 1020

961 cagaacgaca gaaataaatc cccaaaacgc gcgcgccatc cgggccctgg tcgccagagc 1020

.......... .......... ......... Orf frame 1 ......... ..........

.......... .......... .......... .......... .......... ..........

1021 gtcgttgagg gtcctgtgta ttttttccag gacgtggtaa aggtgactct ggatgttcag 1080

1021 cagcaactcc caggacacat aaaaaaggtc ctgcaccatt tccactgaga cctacaagtc 1080

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 atacatgggc ataagcccgt ctctggggtg gaggtagcac cactgcagag cttcatgctg 1140

1081 tatgtacccg tattcgggca gagaccccac ctccatcgtg gtgacgtctc gaagtacgac 1140

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cggggtggtg ttgtagatga tccagtcgta gcaggagcgc tgggcgtggt gcctaaaaat 1200

1141 gccccaccac aacatctact aggtcagcat cgtcctcgcg acccgcacca cggattttta 1200

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gtctttcagt agcaagctga ttgccagggg caggcccttg gtgtaagtgt ttacaaagcg 1260

1201 cagaaagtca tcgttcgact aacggtcccc gtccgggaac cacattcaca aatgtttcgc 1260

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gttaagctgg gatgggtgca tacgtgggga tatgagatgc atcttggact gtatttttag 1320

1261 caattcgacc ctacccacgt atgcacccct atactctacg tagaacctga cataaaaatc 1320

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gttggctatg ttcccagcca tatccctccg gggattcatg ttgtgcagaa ccaccagcac 1380

1321 caaccgatac aagggtcggt atagggaggc ccctaagtac aacacgtctt ggtggtcgtg 1380

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 agtgtatccg gtgcacttgg gaaatttgtc atgtagctta gaaggaaatg cgtggaagaa 1440

1381 tcacataggc cacgtgaacc ctttaaacag tacatcgaat cttcctttac gcaccttctt 1440

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cttggagacg cccttgtgac ctccaagatt ttccatgcat tcgtccataa tgatggcaat 1500

1441 gaacctctgc gggaacactg gaggttctaa aaggtacgta agcaggtatt actaccgtta 1500

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gggcccacgg gcggcggcct gggcgaagat atttctggga tcactaacgt catagttgtg 1560

1501 cccgggtgcc cgccgccgga cccgcttcta taaagaccct agtgattgca gtatcaacac 1560

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ttccaggatg agatcgtcat aggccatttt tacaaagcgc gggcggaggg tgccagactg 1620

1561 aaggtcctac tctagcagta tccggtaaaa atgtttcgcg cccgcctccc acggtctgac 1620

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cggtataatg gttccatccg gcccaggggc gtagttaccc tcacagattt gcatttccca 1680

1621 gccatattac caaggtaggc cgggtccccg catcaatggg agtgtctaaa cgtaaagggt 1680

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cgctttgagt tcagatgggg ggatcatgtc tacctgcggg gcgatgaaga aaacggtttc 1740

1681 gcgaaactca agtctacccc cctagtacag atggacgccc cgctacttct tttgccaaag 1740

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cggggtaggg gagatcagct gggaagaaag caggttcctg agcagctgcg acttaccgca 1800

1741 gccccatccc ctctagtcga cccttctttc gtccaaggac tcgtcgacgc tgaatggcgt 1800

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gccggtgggc ccgtaaatca cacctattac cgggtgcaac tggtagttaa gagagctgca 1860

1801 cggccacccg ggcatttagt gtggataatg gcccacgttg accatcaatt ctctcgacgt 1860

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gctgccgtca tccctgagca ggggggccac ttcgttaagc atgtccctga ctcgcatgtt 1920

1861 cgacggcagt agggactcgt ccccccggtg aagcaattcg tacagggact gagcgtacaa 1920

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttccctgacc aaatccgcca gaaggcgctc gccgcccagc gatagcagtt cttgcaagga 1980

1921 aagggactgg tttaggcggt cttccgcgag cggcgggtcg ctatcgtcaa gaacgttcct 1980

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agcaaagttt ttcaacggtt tgagaccgtc cgccgtaggc atgcttttga gcgtttgacc 2040

1981 tcgtttcaaa aagttgccaa actctggcag gcggcatccg tacgaaaact cgcaaactgg 2040

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 aagcagttcc aggcggtccc acagctcggt cacctgctct acggcatctc gatccagcat 2100

2041 ttcgtcaagg tccgccaggg tgtcgagcca gtggacgaga tgccgtagag ctaggtcgta 2100

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 atctcctcgt ttcgcgggtt ggggcggctt tcgctgtacg gcagtagtcg gtgctcgtcc 2160

2101 tagaggagca aagcgcccaa ccccgccgaa agcgacatgc cgtcatcagc cacgagcagg 2160

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 agacgggcca gggtcatgtc tttccacggg cgcagggtcc tcgtcagcgt agtctgggtc 2220

2161 tctgcccggt cccagtacag aaaggtgccc gcgtcccagg agcagtcgca tcagacccag 2220

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acggtgaagg ggtgcgctcc gggctgcgcg ctggccaggg tgcgcttgag gctggtcctg 2280

2221 tgccacttcc ccacgcgagg cccgacgcgc gaccggtccc acgcgaactc cgaccaggac 2280

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ctggtgctga agcgctgccg gtcttcgccc tgcgcgtcgg ccaggtagca tttgaccatg 2340

2281 gaccacgact tcgcgacggc cagaagcggg acgcgcagcc ggtccatcgt aaactggtac 2340

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gtgtcatagt ccagcccctc cgcggcgtgg cccttggcgc gcagcttgcc cttggaggag 2400

2341 cacagtatca ggtcggggag gcgccgcacc gggaaccgcg cgtcgaacgg gaacctcctc 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgccgcacg aggggcagtg cagacttttg agggcgtaga gcttgggcgc gagaaatacc 2460

2401 cgcggcgtgc tccccgtcac gtctgaaaac tcccgcatct cgaacccgcg ctctttatgg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gattccgggg agtaggcatc cgcgccgcag gccccgcaga cggtctcgca ttccacgagc 2520

2461 ctaaggcccc tcatccgtag gcgcggcgtc cggggcgtct gccagagcgt aaggtgctcg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 caggtgagct ctggccgttc ggggtcaaaa accaggtttc ccccatgctt tttgatgcgt 2580

2521 gtccactcga gaccggcaag ccccagtttt tggtccaaag ggggtacgaa aaactacgca 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttcttacctc tggtttccat gagccggtgt ccacgctcgg tgacgaaaag gctgtccgtg 2640

2581 aagaatggag accaaaggta ctcggccaca ggtgcgagcc actgcttttc cgacaggcac 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tccccgtata cagactnnng tttaaacgaa ttcnnntata aaatgcaagg tgctgctcaa 2700

2641 aggggcatat gtctgannnc aaatttgctt aagnnnatat tttacgttcc acgacgagtt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aaaatcaggc aaagcctcgc gcaaaaaaga aagcacatcg tagtcatgct catgcagata 2760

2701 ttttagtccg tttcggagcg cgttttttct ttcgtgtagc atcagtacga gtacgtctat 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aaggcaggta agctccggaa ccaccacaga aaaagacacc atttttctct caaacatgtc 2820

2761 ttccgtccat tcgaggcctt ggtggtgtct ttttctgtgg taaaaagaga gtttgtacag 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tgcgggtttc tgcataaaca caaaataaaa taacaaaaaa acatttaaac attagaagcc 2880

2821 acgcccaaag acgtatttgt gttttatttt attgtttttt tgtaaatttg taatcttcgg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgtcttacaa caggaaaaac aacccttata agcataagac ggactacggc catgccggcg 2940

2881 acagaatgtt gtcctttttg ttgggaatat tcgtattctg cctgatgccg gtacggccgc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tgaccgtaaa aaaactggtc accgtgatta aaaagcacca ccgacagctc ctcggtcatg 3000

2941 actggcattt ttttgaccag tggcactaat ttttcgtggt ggctgtcgag gagccagtac 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tccggagtca taatgtaaga ctcggtaaac acatcaggtt gattcatcgg tcagtgctaa 3060

3001 aggcctcagt attacattct gagccatttg tgtagtccaa ctaagtagcc agtcacgatt 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aaagcgaccg aaatagcccg ggggaataca tacccgcagg cgtagagaca acattacagc 3120

3061 tttcgctggc tttatcgggc ccccttatgt atgggcgtcc gcatctctgt tgtaatgtcg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccccatagga ggtataacaa aattaatagg agagaaaaac acataaacac ctgaaaaacc 3180

3121 ggggtatcct ccatattgtt ttaattatcc tctctttttg tgtatttgtg gactttttgg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctcctgccta ggcaaaatag caccctcccg ctccagaaca acatacagcg cttcacagcg 3240

3181 gaggacggat ccgttttatc gtgggagggc gaggtcttgt tgtatgtcgc gaagtgtcgc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gcagcctaac agtcagcctt accagtaaaa aagaaaacct attaaaaaaa caccactcga 3300

3241 cgtcggattg tcagtcggaa tggtcatttt ttcttttgga taattttttt gtggtgagct 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cacggcacca gctcaatcag tcacagtgta aaaaagggcc aagtgcagag cgagtatata 3360

3301 gtgccgtggt cgagttagtc agtgtcacat tttttcccgg ttcacgtctc gctcatatat 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 taggactaaa aaatgacgta acggttaaag tccacaaaaa acacccagaa aaccgcacgc 3420

3361 atcctgattt tttactgcat tgccaatttc aggtgttttt tgtgggtctt ttggcgtgcg 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gaacctacgc ccagaaacga aagccaaaaa acccacaact tcctcaaatc gtcacttccg 3480

3421 cttggatgcg ggtctttgct ttcggttttt tgggtgttga aggagtttag cagtgaaggc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ttttcccacg ttacgtaact tcccatttta agaaaactac aattcccaac acatacaagt 3540

3481 aaaagggtgc aatgcattga agggtaaaat tcttttgatg ttaagggttg tgtatgttca 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tactccgccc taaaacctac gtcacccgcc ccgttcccac gccccgcgcc acgtcacaaa 3600

3541 atgaggcggg attttggatg cagtgggcgg ggcaagggtg cggggcgcgg tgcagtgttt 3600

.......... .......... .......... LITR RITR .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ctccaccccc tcattatcat attggcttca atccaaaata aggtatatta ttgatgatnn 3660

3601 gaggtggggg agtaatagta taaccgaagt taggttttat tccatataat aactactann 3660

.......... .......... LITR RITR .......... .......... ..........

BamHI(3670) ........ .......... .......... .......... ..........

3661 nttaattaag gatccnnncg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 3720

3661 naattaattc ctaggnnngc cacactttat ggcgtgtcta cgcattcctc ttttatggcg 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 3780

3721 tagtccgcga gaaggcgaag gagcgagtga ctgagcgacg cgagccagca agccgacgcc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 3840

3781 gctcgccata gtcgagtgag tttccgccat tatgccaata ggtgtcttag tcccctattg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 3900

3841 cgtcctttct tgtacactcg ttttccggtc gttttccggt ccttggcatt tttccggcgc 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 3960

3901 aacgaccgca aaaaggtatc cgaggcgggg ggactgctcg tagtgttttt agctgcgagt 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 4020

3961 tcagtctcca ccgctttggg ctgtcctgat atttctatgg tccgcaaagg gggaccttcg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 4080

4021 agggagcacg cgagaggaca aggctgggac ggcgaatggc ctatggacag gcggaaagag 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 4140

4081 ggaagccctt cgcaccgcga aagagtatcg agtgcgacat ccatagagtc aagccacatc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 4200

4141 cagcaagcga ggttcgaccc gacacacgtg cttggggggc aagtcgggct ggcgacgcgg 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 4260

4201 aataggccat tgatagcaga actcaggttg ggccattctg tgctgaatag cggtgaccgt 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 4320

4261 cgtcggtgac cattgtccta atcgtctcgc tccatacatc cgccacgatg tctcaagaac 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 4380

4321 ttcaccaccg gattgatgcc gatgtgatct tcctgtcata aaccatagac gcgagacgac 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 4440

4381 ttcggtcaat ggaagccttt ttctcaacca tcgagaacta ggccgtttgt ttggtggcga 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 4500

4441 ccatcgccac caaaaaaaca aacgttcgtc gtctaatgcg cgtctttttt tcctagagtt 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 4560

4501 cttctaggaa actagaaaag atgccccaga ctgcgagtca ccttgctttt gagtgcaatt 4560

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 4620

4561 ccctaaaacc agtactctaa tagtttttcc tagaagtgga tctaggaaaa tttaattttt 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 4680

4621 acttcaaaat ttagttagat ttcatatata ctcatttgaa ccagactgtc aatggttacg 4680

.......... .......... .......... .......... .......... Ampicillin

.......... .......... .......... .......... .......... ..........

4681 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 4740

4681 aattagtcac tccgtggata gagtcgctag acagataaag caagtaggta tcaacggact 4740

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 4800

4741 gaggggcagc acatctattg atgctatgcc ctcccgaatg gtagaccggg gtcacgacgt 4800

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 4860

4801 tactatggcg ctctgggtgc gagtggccga ggtctaaata gtcgttattt ggtcggtcgg 4860

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 4920

4861 ccttcccggc tcgcgtcttc accaggacgt tgaaataggc ggaggtaggt cagataatta 4920

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgnn 4980

4921 acaacggccc ttcgatctca ttcatcaagc ggtcaattat caaacgcgtt gcaacaacnn 4980

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 nnnnaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg cagaaacggt 5040

4981 nnnntttttc ctagaagtgg atctaggaaa agtgcatctt tcggtcaggc gtctttgcca 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa 5100

5041 cgactggggc ctacttacag tcgatgaccc gatagacctg ttcccttttg cgttcgcgtt 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat 5160

5101 tctctttcgt ccatcgaacg tcacccgaat gtaccgctat cgatctgacc cgccaaaata 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct 5220

5161 cctgtcgttc gcttggcctt aacggtcgac cccgcgggag accattccaa cccttcggga 5220

.......... .......... ....... NEOKAN_promoter ....... ..........

.......... .......... .......... .......... .......... ..........

5221 gcaaagtaaa ctggatggct ttctcgccgc caaggatctg atggcgcagg ggatcaagct 5280

5221 cgtttcattt gacctaccga aagagcggcg gttcctagac taccgcgtcc cctagttcga 5280

.......... .......... .......... .......... .......... ..........

BclI(5282) .......... .......... .......... .......... ..........

5281 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 5340

5281 gactagttct ctgtcctact cctagcaaag cgtactaact tgttctacct aacgtgcgtc 5340

.......... .......... .......... ..... Orf frame 3 Neomycin .....

.......... .......... .......... .......... .......... ..........

5341 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 5400

5341 caagaggccg gcgaacccac ctctccgata agccgatact gacccgtgtt gtctgttagc 5400

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 5460

5401 cgacgagact acggcggcac aaggccgaca gtcgcgtccc cgcgggccaa gaaaaacagt 5460

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc 5520

5461 tctggctgga caggccacgg gacttacttg acgttctgct ccgtcgcgcc gatagcaccg 5520

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 5580

5521 accggtgctg cccgcaagga acgcgtcgac acgagctgca acagtgactt cgcccttccc 5580

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 5640

5581 tgaccgacga taacccgctt cacggccccg tcctagagga cagtagagtg gaacgaggac 5640

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 5700

5641 ggctctttca taggtagtac cgactacgtt acgccgccga cgtatgcgaa ctaggccgat 5700

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 5760

5701 ggacgggtaa gctggtggtt cgctttgtag cgtagctcgc tcgtgcatga gcctaccttc 5760

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 5820

5761 ggccagaaca gctagtccta ctagacctgc ttctcgtagt ccccgagcgc ggtcggcttg 5820

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg 5880

5821 acaagcggtc cgagttccgc tcgtacgggc tgccgctcct agagcagcac tgggtaccgc 5880

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 5940

5881 tacggacgaa cggcttatag taccaccttt taccggcgaa aagacctaag tagctgacac 5940

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 6000

5941 cggccgaccc acaccgcctg gcgatagtcc tgtatcgcaa ccgatgggca ctataacgac 6000

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 6060

6001 ttctcgaacc gccgcttacc cgactggcga aggagcacga aatgccatag cggcgagggc 6060

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt ttgttaaaat 6120

6061 taagcgtcgc gtagcggaag atagcggaag aactgctcaa gaagacttaa aacaatttta 6120

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaacat cccttataaa 6180

6121 aaaacaattt agtcgagtaa aaaattggtt atccggcttt agccgttgta gggaatattt 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 tcaaaagaat agaccgcgat agggttgagt gttgttccag tttggaacaa gagtccacta 6240

6181 agttttctta tctggcgcta tcccaactca caacaaggtc aaaccttgtt ctcaggtgat 6240

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

6241 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 6300

6241 aatttcttgc acctgaggtt gcagtttccc gctttttggc agatagtccc gctaccgggt 6300

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ctacgtgaac catcacccaa atcaagtttt ttgcggtcga ggtgccgtaa agctctaaat 6360

6301 gatgcacttg gtagtgggtt tagttcaaaa aacgccagct ccacggcatt tcgagattta 6360

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 6420

6361 gccttgggat ttccctcggg ggctaaatct cgaactgccc ctttcggccg cttgcaccgc 6420

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 6480

6421 tctttccttc ccttctttcg ctttcctcgc ccgcgatccc gcgaccgttc acatcgccag 6480

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 acgctgcgcg taaccaccac acccgcgcgc ttaatgcgcc gnnnnnnn.. .......... 6528

6481 tgcgacgcgc attggtggtg tgggcgcgcg aattacgcgg cnnnnnnn.. .......... 6528

.......... f1_origin .......... .......... .......... ..........