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pSico
Gene/insert name: none
Insert size (bp): Unknown
Relevant mutations/deletions: EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. Cre causes EGFP to be recombined out of the construct, activating shRNA expression.
Vector backbone: pSico (Search Vector Database)
Type of vector: Mammalian expression,RNAi
Backbone size (bp): 7566
Cloning site 5': HpaI
Site destroyed during cloning: No
Cloning site 3': XhoI
Site destroyed during cloning: No
5' Sequencing primer: EGFP-C (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Tyler Jacks
Comments: pSico allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS ON shRNA expression. The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites.
Article: Cre-lox-regulated conditional RNA interference from transgenes. Ventura A et al. (Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5. Pubmed)