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pSin-EF2-Sox2-Pur
Gene/insert name: Homo sapiens SRY (sex determining region Y)-box 2 (SOX2)
Alternative names: Sox2
Insert size (bp): 1000
GenBank/Entrez ID of insert: NM_003106
Gene/insert aliases: SOX2, ANOP3, MCOPS3, MGC2413
Species of gene(s): H. sapiens (human)
Vector backbone: pSin4-EF2-IRES-Pur (Search Vector Database)
Type of vector: Mammalian expression,Lentiviral
Backbone size (bp): 7500
Cloning site 5': EcoRI
Site destroyed during cloning: No
Cloning site 3': SpeI
Site destroyed during cloning: No
5' Sequencing primer: EF-1a Forward (List of Sequencing Primers)
3' Sequencing primer: n/a
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Selectable markers: Puromycin
Plasmid Provided In: Stbl3
Principal Investigator: James A Thomson
Comments: The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector.