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    Plasmid map of pSin-EF2-Sox2-Pur

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    pSin-EF2-Sox2-Pur
    Gene/insert name: Homo sapiens SRY (sex determining region Y)-box 2 (SOX2)
    Alternative names: Sox2
    Insert size (bp): 1000
    GenBank/Entrez ID of insert: NM_003106
    Gene/insert aliases: SOX2, ANOP3, MCOPS3, MGC2413
    Species of gene(s): H. sapiens (human)
    Vector backbone: pSin4-EF2-IRES-Pur (Search Vector Database)
    Type of vector: Mammalian expression,Lentiviral
    Backbone size (bp): 7500
    Cloning site 5': EcoRI
    Site destroyed during cloning: No
    Cloning site 3': SpeI
    Site destroyed during cloning: No
    5' Sequencing primer: EF-1a Forward (List of Sequencing Primers)
    3' Sequencing primer: n/a
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Selectable markers: Puromycin
    Plasmid Provided In: Stbl3
    Principal Investigator: James A Thomson
    Comments: The psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector.



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