ZZ\LOCUS pSin-EF2-Sox2-Pur Sequence 8496 bp DNA SYN 12-Dec-2007 DEFINITION pSin-EF2-Sox2-Pur Sequence ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..8496 /organism="pSin-EF2-Sox2-Pur Sequence" /mol_type="other DNA" misc_feature 570..750 /label="HIV-1_5_LTR" misc_feature 570..750 /label="truncHIV-1_3_LTR" misc_feature 861..905 /label="HIV-1_psi_pack" misc_feature 1415..1648 /label="RRE" CDS 1293..2213 /label="ORF frame 3" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSRRYRHKWQYSSTILKERGGLGGTVQGKE*" promoter 2416..3587 /label="EF1a_promoter" CDS complement(2707..3327) /label="ORF frame 1" /translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSSLQQGREAAIFPL TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP RRRPRPQKPKYQCADLGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR GGPSAQARPQLKHEAKGLLKRKASNSPTHFQPEARDQESRTAARWK*" misc_feature 3535..3555 /label="EF1a_fwd_primer" CDS 3603..4556 /label="ORF frame 3" /translation="MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRP MNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHM KEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSY AHMNGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMN GSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISM YLPGAEVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM*" misc_feature 4611..5174 /label="IRES" CDS 5095..5817 /label="puro(0-600)" /gene="puro(0-600)" /note="ORF frame 1" /translation="MLYMCLVEVKKTSRPPEPRGRGFPLKNTMISLPQPTRRRPSMTE YKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDI GKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPK EPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFT VTADVECPKDRATWCMTRKPGA*" gene 5218..5817 /label="puro(0-600)" /gene="puro(0-600)" misc_feature 6003..6018 /label="cPPT" misc_feature 6003..6024 /label="U3PPT" misc_feature 6020..6072 /label="delta_U3" misc_feature 6073..6253 /label="HIV-1_5_LTR" misc_feature 6073..6253 /label="truncHIV-1_3_LTR" misc_feature complement(6404..6421) /label="BGH_rev_primer" terminator 6407..6634 /label="bGH_PA_terminator" promoter 6765..6793 /label="AmpR_promoter" CDS 6835..7695 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 6835..7695 /label="Ampicillin" /gene="Ampicillin" rep_origin 7850..8469 /label="pBR322_origin" ORIGIN 1 cggaccgcca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa 61 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga 121 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca 181 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac 241 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa 301 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag 361 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct 421 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg 481 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg 541 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct 601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 721 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa 781 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag 841 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga 901 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa 961 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag 1021 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag 1081 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt 1141 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa 1201 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc 1261 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat 1321 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa 1381 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg 1441 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat 1501 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc 1561 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg 1621 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt 1681 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga 1741 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact 1801 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag 1861 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat 1921 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta 1981 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc 2041 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca 2101 gatccattcg attagtgaac ggatctcgac ggtatcgcca caaatggcag tattcatcca 2161 caattttaaa agaaaggggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 2221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 2281 tcgggtttat tacagggaca gcagagatcc actttggtcg ataagctttg caaagatgga 2341 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag 2401 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga 2461 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact 2521 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata 2581 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg 2641 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc 2701 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa 2761 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg 2821 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc 2881 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt 2941 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt 3001 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg 3061 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc 3121 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt 3181 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa 3241 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg 3301 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc 3361 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt 3421 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact 3481 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc 3541 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga attcgattcc 3601 gcatgtacaa catgatggag acggagctga agccgccggg cccgcagcaa acttcggggg 3661 gcggcggcgg caactccacc gcggcggcgg ccggcggcaa ccagaaaaac agcccggacc 3721 gcgtcaagcg gcccatgaat gccttcatgg tgtggtcccg cgggcagcgg cgcaagatgg 3781 cccaggagaa ccccaagatg cacaactcgg agatcagcaa gcgcctgggc gccgagtgga 3841 aacttttgtc ggagacggag aagcggccgt tcatcgacga ggctaagcgg ctgcgagcgc 3901 tgcacatgaa ggagcacccg gattataaat accggccccg gcggaaaacc aagacgctca 3961 tgaagaagga taagtacacg ctgcccggcg ggctgctggc ccccggcggc aatagcatgg 4021 cgagcggggt cggggtgggc gccggcctgg gcgcgggcgt gaaccagcgc atggacagtt 4081 acgcgcacat gaacggctgg agcaacggca gctacagcat gatgcaggac cagctgggct 4141 acccgcagca cccgggcctc aatgcgcacg gcgcagcgca gatgcagccc atgcaccgct 4201 acgacgtgag cgccctgcag tacaactcca tgaccagctc gcagacctac atgaacggct 4261 cgcccaccta cagcatgtcc tactcgcagc agggcacccc tggcatggct cttggctcca 4321 tgggttcggt ggtcaagtcc gaggccagct ccagcccccc tgtggttacc tcttcctccc 4381 actccagggc gccctgccag gccggggacc tccgggacat gatcagcatg tatctccccg 4441 gcgccgaggt gccggaaccc gccgccccca gcagacttca catgtcccag cactaccaga 4501 gcggcccggt gcccggcacg gccattaacg gcacactgcc cctctcacac atgtgagggc 4561 cggaaatcac tagttcgaag gatccgcatg catctagggc ggccaattcc gcccctctcc 4621 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt 4681 ctatatgtga ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg 4741 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg 4801 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc 4861 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca 4921 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag 4981 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa 5041 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt 5101 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt 5161 tttcctttga aaaacacgat gataagcttg ccacaaccca caaggagacg accttccatg 5221 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc 5281 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc 5341 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 5401 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 5461 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 5521 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 5581 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 5641 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 5701 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 5761 gtcgagtgcc cgaaggaccg cgcgacctgg tgcatgaccc gcaagcccgg tgcctgataa 5821 taggcggccg ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 5881 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc 5941 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 6001 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt 6061 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 6121 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 6181 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 6241 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 6301 aaatgaatat cagagagtga gaggccttga cattataata gatttagcag gaattgaact 6361 aggagtggag cacacaggca aagttctaga gctcgctgat cagcctcgac tgtgccttct 6421 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc 6481 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt 6541 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat 6601 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg 6661 gggcgcgccc ctcgaggccg ccatggtcat agctgtttga cgtcaggtgg cacttttcgg 6721 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 6781 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 6841 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 6901 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 6961 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 7021 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 7081 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 7141 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 7201 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 7261 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 7321 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 7381 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 7441 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 7501 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 7561 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 7621 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 7681 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 7741 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 7801 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 7861 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 7921 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 7981 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 8041 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 8101 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 8161 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 8221 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc 8281 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 8341 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 8401 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 8461 agcaacgcgg cctttttacg gttcctggcc ttttgc // Reverse complement: gcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaa atcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtg cgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatg ctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccg accgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccact ggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactag aaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaac aaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcct ttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaag gatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgaca gttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtc gtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggc tccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatcc agtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctaca ggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatc ccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcac tcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactca accaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccaca tagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagat ccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaa acaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaata ttattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaatagggg ttccgcgcacatttccccgaaaagtgccacctgacgtcaaacagctatgaccatggcggcctcgaggggcgcgcccccag ctggttctttccgcctcagaagccatagagcccaccgcatccccagcatgcctgctattgtcttcccaatcctccccctt gctgtcctgccccaccccaccccccagaatagaatgacacctactcagacaatgcgatgcaatttcctcattttattagg aaaggacagtgggagtggcaccttccagggtcaaggaaggcacgggggaggggcaaacaacagatggctggcaactagaa ggcacagtcgaggctgatcagcgagctctagaactttgcctgtgtgctccactcctagttcaattcctgctaaatctatt ataatgtcaaggcctctcactctctgatattcatttctttgcaagttataaatactgaataataagatgacatgaactac tactgctagagattttccacactgactaaaagggtctgagggatctctagttaccagagtcacacaacagacgggcacac actacttgaagcactcaaggcaagctttattgaggcttaagcagtgggttccctagttagccagagagctcccaggctca gatctggtctaaccagagagacccagtacaagcaaaaagcagatcttgtcttcgttgggagtgaattagcccttccagtc cccccttttcttttaaaaagtggctaagatctacagctgccttgtaagtcattggtcttaaaggtacctgaggtgtgact ggaaaacccacctcctcctcctcttgtgcttctagccaggcacaatcagcattggtagctgctgtattgctacttgtgat tgctccatgtttttctaggtctcgagcggccgcctattatcaggcaccgggcttgcgggtcatgcaccaggtcgcgcggt ccttcgggcactcgacgtcggcggtgacggtgaagccgagccgctcgtagaaggggaggttgcggggcgcggaggtctcc aggaaggcgggcaccccggcgcgctcggccgcctccactccggggagcacgacggcgctgcccagacccttgccctggtg gtcgggcgagacgccgacggtggccaggaaccacgcgggctccttgggccggtgcggcgccaggaggccttccatctgtt gctgcgcggccagccgggaaccgctcaactcggccatgcgcgggccgatctcggcgaacaccgcccccgcttcgacgctc tccggcgtggtccagaccgccaccgcggcgccgtcgtccgcgacccacaccttgccgatgtcgagcccgacgcgcgtgag gaagagttcttgcagctcggtgacccgctcgatgtggcggtccgggtcgacggtgtggcgcgtggcggggtagtcggcga acgcggcggcgagggtgcgtacggcccgggggacgtcgtcgcgggtggcgaggcgcaccgtgggcttgtactcggtcatg gaaggtcgtctccttgtgggttgtggcaagcttatcatcgtgtttttcaaaggaaaaccacgtccccgtggttcgggggg cctagacgtttttttaacctcgactaaacacatgtaaagcatgtgcaccgaggccccagatcagatcccatacaatgggg taccttctgggcatccttcagccccttgttgaatacgcttgaggagagccatttgactctttccacaactatccaactca caacgtggcactggggttgtgccgcctttgcaggtgtatcttatacacgtggcttttggccgcagaggcacctgtcgcca ggtggggggttccgctgcctgcaaagggtcgctacagacgttgtttgtcttcaagaagcttccagaggaactgcttcctt cacgacattcaacagaccttgcattcctttggcgagaggggaaagacccctaggaatgctcgtcaagaagacagggccag gtttccgggccctcacattgccaaaagacggcaatatggtggaaaatcacatatagacaaacgcacaccggccttattcc aagcggcttcggccagtaacgttaggggggggggagggagaggggcggaattggccgccctagatgcatgcggatccttc gaactagtgatttccggccctcacatgtgtgagaggggcagtgtgccgttaatggccgtgccgggcaccgggccgctctg gtagtgctgggacatgtgaagtctgctgggggcggcgggttccggcacctcggcgccggggagatacatgctgatcatgt cccggaggtccccggcctggcagggcgccctggagtgggaggaagaggtaaccacaggggggctggagctggcctcggac ttgaccaccgaacccatggagccaagagccatgccaggggtgccctgctgcgagtaggacatgctgtaggtgggcgagcc gttcatgtaggtctgcgagctggtcatggagttgtactgcagggcgctcacgtcgtagcggtgcatgggctgcatctgcg ctgcgccgtgcgcattgaggcccgggtgctgcgggtagcccagctggtcctgcatcatgctgtagctgccgttgctccag ccgttcatgtgcgcgtaactgtccatgcgctggttcacgcccgcgcccaggccggcgcccaccccgaccccgctcgccat gctattgccgccgggggccagcagcccgccgggcagcgtgtacttatccttcttcatgagcgtcttggttttccgccggg gccggtatttataatccgggtgctccttcatgtgcagcgctcgcagccgcttagcctcgtcgatgaacggccgcttctcc gtctccgacaaaagtttccactcggcgcccaggcgcttgctgatctccgagttgtgcatcttggggttctcctgggccat cttgcgccgctgcccgcgggaccacaccatgaaggcattcatgggccgcttgacgcggtccgggctgtttttctggttgc cgccggccgccgccgcggtggagttgccgccgccgccccccgaagtttgctgcgggcccggcggcttcagctccgtctcc atcatgttgtacatgcggaatcgaattcctcacgacacctgaaatggaagaaaaaaactttgaaccactgtctgaggctt gagaatgaaccaagatccaaactcaaaaagggcaaattccaaggagaattacatcaagtgccaagctggcctaacttcag tctccacccactcagtgtggggaaactccatcgcataaaacccctccccccaacctaaagacgacgtactccaaaagctc gagaactaatcgaggtgcctggacggcgcccggtactccgtggagtcacatgaagcgacggctgaggacggaaaggccct tttcctttgtgtgggtgactcacccgcccgctctcccgagcgccgcgtcctccattttgagctccctgcagcagggccgg gaagcggccatctttccgctcacgcaactggtgccgaccgggccagccttgccgcccagggcggggcgatacacggcggc gcgaggccaggcaccagagcaggccggccagcttgagactacccccgtccgattctcggtggccgcgctcgcaggccccg cctcgccgaacatgtgcgctgggacgcacgggccccgtcgccgcccgcggccccaaaaaccgaaataccagtgtgcagat cttggcccgcatttacaagactatcttgccagaaaaaaagcgtcgcagcaggtcatcaaaaattttaaatggctagagac ttatcgaaagcagcgagacaggcgcgaaggtgccaccagattcgcacgcggcggccccagcgcccaggccaggcctcaac tcaagcacgaggcgaaggggctccttaagcgcaaggcctcgaactctcccacccacttccaacccgaagctcgggatcaa gaatcacgtactgcagccaggtggaagtaattcaaggcacgcaagggccataacccgtaaagaggccaggcccgcgggaa ccacacacggcacttacctgtgttctggcggcaaacccgttgcgaaaaagaacgttcacggcgactactgcacttatata cggttctcccccaccctcgggaaaaaggcggagccagtacacgacatcactttcccagtttaccccgcgccaccttctct aggcaccggttcaattgccgacccctccccccaacttctcggggactgtgggcgatgtgcgctctgcccactgacgggca ccggagccaattcccactcctttcaagacctagaaggtccattagctgcaaagattcctctctgtttaaaactttatcca tctttgcaaagcttatcgaccaaagtggatctctgctgtccctgtaataaacccgaaaattttgaatttttgtaatttgt ttttgtaattctttagtttgtatgtctgttgctattatgtctactattctttcccctgcactgtaccccccaatcccccc ctttcttttaaaattgtggatgaatactgccatttgtggcgataccgtcgagatccgttcactaatcgaatggatctgtc tctgtctctctctccaccttcttcttctattccttcgggcctgtcgggtcccctcggggttgggaggtgggtctgaaacg ataatggtgaatatccctgcctaactctattcactatagaaagtacagcaaaaactattcttaaacctaccaagcctcct actatcattatgaataattttatataccacagccaatttgttatgttaaaccaattccacaaacttgcccatttatctaa ttccaataattcttgttcattcttttcttgctggttttgcgattcttcaattaaggagtgtattaagcttgtgtaattgt taatttctctgtcccactccatccaggtcgtgtgattccaaatctgttccagagatttattactccaactagcattccaa ggcacagcagtggtgcaaatgagttttccagagcaaccccaaatccccaggagctgttgatcctttaggtatctttccac agccaggattcttgcctggagctgcttgatgccccagactgtgagttgcaacagatgctgttgcgcctcaatagccctca gcaaattgttctgctgctgcactataccagacaataattgtctggcctgtaccgtcagcgtcattgacgctgcgcccata gtgcttcctgctgctcccaagaacccaaggaacaaagctcctattcccactgctcttttttctctctgcaccactcttct ctttgccttggtgggtgctactcctaatggttcaatttttactactttatatttatataattcacttctccaattgtccc tcatatctcctcctccaggtctgaagatcagcggccgcttgctgtgcggtggtcttacttttgttttgctcttcctctat cttgtctaaagcttccttggtgtcttttatctctatcctttgatgcacacaatagagggttgctactgtattatataatg atctaagttcttctgatcctgtctgaagggatggttgtagctgtcccagtatttgtctacagccttctgatgtttctaac aggccaggattaactgcgaatcgttctagctccctgcttgcccatactatatgttttaatttatattttttctttccccc tggccttaaccgaattttttcccatcgcgatctaattctcccccgcttaatactgacgctctcgcacccatctctctcct tctagcctccgctagtcaaaatttttggcgtactcaccagtcgccgcccctcgcctcttgccgtgcgcgcttcagcaagc cgagtcctgcgtcgagagagctcctctggtttccctttcgctttcaagtccctgttcgggcgccactgctagagattttc cacactgactaaaagggtctgagggatctctagttaccagagtcacacaacagacgggcacacactacttgaagcactca aggcaagctttattgaggcttaagcagtgggttccctagttagccagagagctcccaggctcagatctggtctaaccaga gagacccagtacaggcaaaaagcagctgcttatatgcaggatctgagggctcgccactccccagtcccgcccaggccacg cctccctggaaagtccccagcggaaagtcccttgtagcaagctcgatatcagcagttcttgaagtactccggatgcagct ctcgggccacgtgatgaaatgctaggcggctgtcaaacctccactctaacacttctctctccgggtcatccatcccatgc aggctcacagggtgtaacaagctggtgttctctcctttattggcctcttctaccttatctggctcaactggtactagctt gtagcaccatccaaaggtcagtggatatctgacccctggccctggtgtgtagttctgctaatcagggaagtagccttgtg tgtggtagatccacagatcaaggatatcttgtcttctttgggagtgaattagcccttccaactactaagtttgtagtaca tatttaacaaatacaatttctttaaaatgaaaataattcagaggaatcacaggtttagagtaaatgaaaccacaggtaat tggcagtggcggtccg 4!pSin-EF2-Sox2-Pur "Times New Roman!ZC4!:HIV"Times New RomanZC4!:truncHIV"Times New RomanZCɆ4!]HIV-1"Times New RomanZC4! ORF3"Times New RomanZ4!pRRE"Times New RomanZC4!p EF1a_pro"Times New RomanZCɆ4! ORF1"Times New RomanZ4!  EF1a_f_pri"Times New RomanZC4!ORF3"Times New RomanZC4!6IRES"Times New RomanZCɆ4!ORF1"Times New RomanZ4!b puro(0-600)"Times New RomanZC4!sU3PPT"Times New RomanZC4!cPPT"Times New RomanZCɆ4!delta_U3"Times New Roman)Z4!mHIV"Times New RomanZC4!mtruncHIV"Times New RomanZC4!BGH_r"Times New RomanZCɆ4! bGH_PA_term"Times New RomanZ4!mAmpR_pro"Times New RomanZC4!ORF1"Times New RomanZC4! Ampicillin"Times New RomanZCɆ4!! pBR322_origin"Times New Roman ZNruI"Times New Roman*.4!MZ|flf]l ZAgeI"Times New Roman 5 4!hmrky ZEcoRI"Times New RomanL 4!'1:]  Z$BamHI(4580), BstBI(4575), SpeI(4569)"Times New Roman^4!}!0!}N ZSalI"Times New Romanr 4!*   ZMscI"Times New Romanz 4! ZXbaI"Times New RomanS4!1 ZAseI"Times New Roman 4!* {l { Ztest"Times New RomanA4!NLA u Ztest"Times New Roman4!.v8eBSB&S