wmwmmID PT7T318 preliminary; circular DNA; SYN; 2239 BP. XX AC L08951; VB0117; IG0140; XX DT 02-NOV-1992 (Rel. 7, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE E. coli phagemid vector pT7T3-18 - complete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RP 1-2239 RC pT7T3-18 RA Gilbert W.; RT "Obtained from VecBase 3.0"; RL Unpublished (1991). XX CC These data and their annotation were supplied to GenBank by Will CC Gilbert under the auspices of the GenBank Currator Program. CC CC old BRL vector. CC NM (pT7T3-18) CC CM (yes) CC NA (ds-DNA) CC TP (circular) CC ST () CC TY (phagemid) CC SP () CC HO (E.coli) CC CP () CC FN (cloning) CC SE () CC PA (pBR322)(pUC18)(PromT7)(PromT3) CC BR (pT7T3-19) CC OF (pT7T3alpha-18) CC OR () XX FH Key Location/Qualifiers FH FT misc_feature 0..0 FT /note="1. pUC18c 2686bp, ori/amp gene FT 2. pBR322 FT 3. T3, promoter FT 4. T7, promoter FT -> pT7T3-18 2239bp" FT promoter 41..60 FT /note="PRO bacteriophage T3" FT misc_feature complement(76..132) FT /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-PstI- FT SphI-HindIII; M13mp18/pUC18-polylinker" FT promoter complement(139..158) FT /note="PRO bacteriophage T7" FT misc_feature 169..2229 FT /note="from pUC18c" FT misc_feature 223..2237 FT /note="from pBR322" FT rep_origin 0..0 FT /note="ORI E. coli pMB1 (ColE1 and pBR322)" FT misc_feature complement(1172..1960) FT /note="ANT E. coli beta-lactamase gene (bla) FT ampicillin resistance gene (apr/amp)" XX SQ Sequence 2239 BP; 572 A; 563 C; 531 G; 573 T; 0 other; aattctcatg tttgacagct tatcatcgac tccagtttct aattaaccct cactaaaggg agagaccata gatgcaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc gagctcgaat tccggtctcc ctatagtgag tcgtattaat ttcgataagc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatt //  pT7T3-18 "Times New Roman wmC)< T3_promoter"Times New RomanwmC T7_promoter"Times New RomanwmCɆ pBR322_origin"Times New Romanwm Orf frame 2"Times New Roman<wmC Ampicillin"Times New RomanwmC3 AmpR_promoter"Times New Roman wmoHindIII(76), PstI(92), SalI(94), XbaI(100), BamHI(106), SmaI(113), KpnI(119), SacI(125), EcoRI(127), PvuII(173)"Times New Romanj?A_e wmFspI"Times New Roman ''6 wmAatII"Times New Romant nidC