}?:}?:#LOCUS pT7T3-18U 2883 bp DNA SYN 03-Oct-2007 DEFINITION pT7T3-18U ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..2883 /organism="pT7T3-18U" /mol_type="other DNA" misc_feature complement(1..156) /label="lacZ_a" misc_feature complement(1..156) /label="lacZ_a" misc_feature 127..149 /label="M13_pUC_fwd_primer" misc_feature 142..158 /label="M13_forward20_primer" promoter 172..191 /label="T3_promoter" promoter complement(252..270) /label="T7_promoter" misc_feature complement(281..299) /label="M13_reverse_primer" misc_feature complement(298..320) /label="M13_pUC_rev_primer" promoter complement(334..363) /label="lac_promoter" rep_origin complement(672..1291) /label="pBR322_origin" CDS complement(1446..2306) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(1446..2306) /label="Ampicillin" /gene="Ampicillin" promoter complement(2348..2376) /label="AmpR_promoter" rep_origin complement(2570..2866) /label="f1_origin" ORIGIN 1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 121 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctatat aaattaaccc 181 tcactaaagg gaataagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg 241 agctcgaatt ccctatagtg agtcgtatta aattcgtaat catggtcata gctgtttcct 301 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 361 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 421 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 481 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 541 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 601 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 661 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 721 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 781 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 841 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat 901 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 961 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1021 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 1081 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 1141 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 1201 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 1261 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 1321 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 1381 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 1441 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 1501 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 1561 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 1621 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 1681 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 1741 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 1801 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 1861 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 1921 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 1981 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 2041 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 2101 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 2161 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 2221 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 2281 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 2341 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 2401 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa ttgtaaacgt taatgttttg 2461 ttaaatttcg cgttaaatat ttgttaaatc agcttatttt ttaaccagta agcagaaaat 2521 gacaaaaatc cttataaatc aaaagaatag accgagttag ttgtgagtgt tgttccagtt 2581 tggaacaaga gtccactatt aaagaacgtg gactccaacg taaaaccgtc tatcagggcg 2641 atggcccact acgtgaacca tcacccaaat caagtttttg gaggtcgagg tgccgtaaag 2701 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 2761 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 2821 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctactgggcg 2881 cgt //C pT7T3-18U "Times New Roman}?:CC lacZ_a"Times New Roman}?:CC lacZ_a"Times New Roman}?:CɆC M13_pUC_fwd_primer"Times New Roman}?:C M13_forward20_primer"Times New Roman)}?:CC T3_pro"Times New Roman}?:CC T7_pro"Times New Roman}?:CɆC +M13_reverse_primer"Times New Roman}?:C *@M13_pUC_rev_primer"Times New Roman}?:CC Nklac_pro"Times New Roman}?:CC   pBR322_origin"Times New Roman}?:CɆC   Orf frame 3"Times New Roman}?:C   Ampicillin"Times New Roman}?:CC , H  AmpR_promoter"Times New Roman}?:CC 2  f1_origin"Times New Roman }?:fHindIII(195), PstI(211), SalI(213), XbaI(219), BamHI(225), SmaI(232), KpnI(238), SacI(244), EcoRI(246)"Times New Roman*2C w4Q }?:test"Times New RomanC L