Sequence of pT7T3-18U

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Sequence of pT7T3-18U

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Sequence of pT7T3-18U : 2883 BP; 747 A; 710 C; 716 G; 710 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 60

1 gggtaagcgg taagtccgac gcgttgacaa cccttcccgc tagccacgcc cggagaagcg 60

.......... ........ lacZ_a lacZ_a ........ .......... ..........

.......... .......... .......... .......... .......... ..........

61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 120

61 ataatgcggt cgaccgcttt ccccctacac gacgttccgc taattcaacc < font color="#000000" style="font-size:10px" face="Courier">cattgcggtc 120

.......... ........ lacZ_a lacZ_a ........ .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctatat aaattaaccc 180

121 ccaaaagggt cagtgctgca acattttgct gccggtcacg gttcgatata tttaattggg 180

lacZ_a lacZ_a M13_pUC_fwd_primer M13_forward20_primer T3_pro

........ HindIII PstI,SalI) XbaI(219) BamHI(225) SmaI(232)

181 tcactaaagg gaataagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg 240

181 agtgatttcc cttattcgaa cgtacggacg tccagctgag atctcctagg ggcccatggc 240

T3_pro .......... .......... .......... .......... ..........

SacI(244),EcoRI(246) .......... .......... .......... ..........

241 agctcgaatt ccctatagtg agtcgtatta aattcgtaat catggtcata gctgtttcct 300

241 tcgagcttaa gggatatcac tcagcataat ttaagcatta gtaccagtat cgacaaagga 300

.......... .......... T7_pro ...... M13_reverse_primer ......

.......... .......... .......... .......... .......... ..........

301 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 360

301 cacactttaa caataggcga gtgttaaggt gtgttgtatg ctcggccttc gtatttcaca 360

M13_pUC_rev_primer .. .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

361 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 420

361 tttcggaccc cacggattac tcactcgatt gagtgtaatt aacgcaacgc gagtgacggg 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 480

421 cgaaaggtca gccctttgga cagcacggtc gacgtaatta cttagccggt tgcgcgcccc 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 540

481 tctccgccaa acgcataacc cgcgagaagg cgaaggagcg agtgactgag cgacgcgagc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 600

541 cagcaagccg acgccgctcg ccatagtcga gtgagtttcc gccattatgc caataggtgt 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 660

601 cttagtcccc tattgcgtcc tttcttgtac actcgttttc cggtcgtttt ccggtccttg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 720

661 gcatttttcc ggcgcaacga ccgcaaaaag gtatccgagg cggggggact gctcgtagtg 720

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

721 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 780

721 tttttagctg cgagttcagt ctccaccgct ttgggctgtc ctgatatttc tatggtccgc 780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 840

781 aaagggggac cttcgaggga gcacgcgaga ggacaaggct gggacggcga atggcctatg 840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat 900

841 gacaggcgga aagagggaag cccttcgcac cgcgaaagag ttacgagtgc gacatccata 900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 960

901 gagtcaagcc acatccagca agcgaggttc gacccgacac acgtgcttgg ggggcaagtc 960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1020

961 gggctggcga cgcggaatag gccattgata gcagaactca ggttgggcca ttctgtgctg 1020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 1080

1021 aatagcggtg accgtcgtcg gtgaccattg tcctaatcgt ctcgctccat acatccgcca 1080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 1140

1081 cgatgtctca agaacttcac caccggattg atgccgatgt gatcttcttg tcataaacca 1140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 1200

1141 tagacgcgag acgacttcgg tcaatggaag cctttttctc aaccatcgag aactaggccg 1200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 1260

1201 tttgtttggt ggcgaccatc gccaccaaaa aaacaaacgt tcgtcgtcta atgcgcgtct 1260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 1320

1261 ttttttccta gagttcttct aggaaactag aaaagatgcc ccagactgcg agtcaccttg 1320

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 1380

1321 cttttgagtg caattcccta aaaccagtac tctaatagtt tttcctagaa gtggatctag 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 1440

1381 gaaaatttaa tttttacttc aaaatttagt tagatttcat atatactcat ttgaaccaga 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 1500

1441 ctgtcaatgg ttacgaatta gtcactccgt ggatagagtc gctagacaga taaagcaagt 1500

.......... .......... .... Orf frame 3 Ampicillin .... ..........

.......... .......... .......... .......... .......... ..........

1501 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 1560

1501 aggtatcaac ggactgaggg gcagcacatc tattgatgct atgccctccc gaatggtaga 1560

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 1620

1561 ccggggtcac gacgttacta tggcgctctg ggtgcgagtg gccgaggtct aaatagtcgt 1620

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 1680

1621 tatttggtcg gtcggccttc ccggctcgcg tcttcaccag gacgttgaaa taggcggagg 1680

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 1740

1681 taggtcagat aattaacaac ggcccttcga tctcattcat caagcggtca attatcaaac 1740

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 1800

1741 gcgttgcaac aacggtaacg atgtccgtag caccacagtg cgagcagcaa accataccga 1800

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 1860

1801 agtaagtcga ggccaagggt tgctagttcc gctcaatgta ctagggggta caacacgttt 1860

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 1920

1861 tttcgccaat cgaggaagcc aggaggctag caacagtctt cattcaaccg gcgtcacaat 1920

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 1980

1921 agtgagtacc aataccgtcg tgacgtatta agagaatgac agtacggtag gcattctacg 1980

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 2040

1981 aaaagacact gaccactcat gagttggttc agtaagactc ttatcacata cgccgctggc 2040

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 2100

2041 tcaacgagaa cgggccgcag ttatgcccta ttatggcgcg gtgtatcgtc ttgaaatttt 2100

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 2160

2101 cacgagtagt aaccttttgc aagaagcccc gcttttgaga gttcctagaa tggcgacaac 2160

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 2220

2161 tctaggtcaa gctacattgg gtgagcacgt gggttgacta gaagtcgtag aaaatgaaag 2220

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 2280

2221 tggtcgcaaa gacccactcg tttttgtcct tccgttttac ggcgtttttt cccttattcc 2280

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 2340

2281 cgctgtgcct ttacaactta tgagtatgag aaggaaaaag ttataataac ttcgtaaata 2340

.... Orf frame 3 Ampicillin .... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 2400

2341 gtcccaataa cagagtactc gcctatgtat aaacttacat aaatcttttt atttgtttat 2400

.......... ........ AmpR_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa ttgtaaacgt taatgttttg 2460

2401 ccccaaggcg cgtgtaaagg ggcttttcac ggtggacttt aacatttgca attacaaaac 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttaaatttcg cgttaaatat ttgttaaatc agcttatttt ttaaccagta agcagaaaat 2520

2461 aatttaaagc gcaatttata aacaatttag tcgaataaaa aattggtcat tcgtctttta 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gacaaaaatc cttataaatc aaaagaatag accgagttag ttgtgagtgt tgttccagtt 2580

2521 ctgtttttag gaatatttag ttttcttatc tggctcaatc aacactcaca acaaggtcaa 2580

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

2581 tggaacaaga gtccactatt aaagaacgtg gactccaacg taaaaccgtc tatcagggcg 2640

2581 accttgttct caggtgataa tttcttgcac ctgaggttgc attttggcag atagtcccgc 2640

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 atggcccact acgtgaacca tcacccaaat caagtttttg gaggtcgagg tgccgtaaag 2700

2641 taccgggtga tgcacttggt agtgggttta gttcaaaaac ctccagctcc acggcatttc 2700

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 2760

2701 gtgatttagc cttgggattt ccctcggggg ctaaatctcg aactgcccct ttcggccgct 2760

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 2820

2761 tgcaccgctc tttccttccc ttctttcgct ttcctcgccc gcgatcccgc gaccgttcac 2820

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctactgggcg 2880

2821 atcgccagtg cgacgcgcat tggtggtgtg ggcggcgcga attacgcggc gatgacccgc 2880

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgt....... .......... .......... .......... .......... .......... 2883

2881 gca....... .......... .......... .......... .......... .......... 2883

.......... .......... .......... .......... .......... ..........