Sequence of pT7T3-18

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Sequence of pT7T3-18

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Sequence of pT7T3-18 : 2239 BP; 572 A; 563 C; 531 G; 573 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aattctcatg tttgacagct tatcatcgac tccagtttct aattaaccct cactaaaggg 60

1 ttaagagtac aaactgtcga atagtagctg aggtcaaaga ttaattggga gtgatttccc 60

.......... .......... .......... ......... T3_promoter .........

......... HindIII PstI,SalI) XbaI(100) BamHI(106) SmaI(113)

61 agagaccata gatgcaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc 120

61 tctctggtat ctacgttcga acgtacggac gtccagctga gatctcctag gggcccatgg 120

.......... .......... .......... .......... .......... ..........

SacI(125),EcoRI(127) .......... .......... .......... PvuII(173)

121 gagctcgaat tccggtctcc ctatagtgag tcgtattaat ttcgataagc cagctgcatt 180

121 ctcgagctta aggccagagg gatatcactc agcataatta aagctattcg gtcgacgtaa 180

.......... ......... T7_promoter ......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 240

181 ttacttagcc ggttgcgcgc ccctctccgc caaacgcata acccgcgaga aggcgaagga 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 300

241 gcgagtgact gagcgacgcg agccagcaag ccgacgccgc tcgccatagt cgagtgagtt 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 360

301 tccgccatta tgccaatagg tgtcttagtc ccctattgcg tcctttcttg tacactcgtt 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 420

361 ttccggtcgt tttccggtcc ttggcatttt tccggcgcaa cgaccgcaaa aaggtatccg 420

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 480

421 aggcgggggg actgctcgta gtgtttttag ctgcgagttc agtctccacc gctttgggct 480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 540

481 gtcctgatat ttctatggtc cgcaaagggg gaccttcgag ggagcacgcg agaggacaag 540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 600

541 gctgggacgg cgaatggcct atggacaggc ggaaagaggg aagcccttcg caccgcgaaa 600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 660

601 gagtatcgag tgcgacatcc atagagtcaa gccacatcca gcaagcgagg ttcgacccga 660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 720

661 cacacgtgct tggggggcaa gtcgggctgg cgacgcggaa taggccattg atagcagaac 720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 780

721 tcaggttggg ccattctgtg ctgaatagcg gtgaccgtcg tcggtgacca ttgtcctaat 780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 840

781 cgtctcgctc catacatccg ccacgatgtc tcaagaactt caccaccgga ttgatgccga 840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 900

841 tgtgatcttc ctgtcataaa ccatagacgc gagacgactt cggtcaatgg aagccttttt 900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 960

901 ctcaaccatc gagaactagg ccgtttgttt ggtggcgacc atcgccacca aaaaaacaaa 960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 1020

961 cgttcgtcgt ctaatgcgcg tctttttttc ctagagttct tctaggaaac tagaaaagat 1020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 1080

1021 gccccagact gcgagtcacc ttgcttttga gtgcaattcc ctaaaaccag tactctaata 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 1140

1081 gtttttccta gaagtggatc taggaaaatt taatttttac ttcaaaattt agttagattt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 1200

1141 catatatact catttgaacc agactgtcaa tggttacgaa ttagtcactc cgtggataga 1200

.......... .......... .......... .... Orf frame 2 Ampicillin ....

.......... .......... .......... .......... .......... ..........

1201 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 1260

1201 gtcgctagac agataaagca agtaggtatc aacggactga ggggcagcac atctattgat 1260

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 1320

1261 gctatgccct cccgaatggt agaccggggt cacgacgtta ctatggcgct ctgggtgcga 1320

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 1380

1321 gtggccgagg tctaaatagt cgttatttgg tcggtcggcc ttcccggctc gcgtcttcac 1380

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 1440

1381 caggacgttg aaataggcgg aggtaggtca gataattaac aacggccctt cgatctcatt 1440

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... FspI(1464) .......... .......... ..........

1441 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 1500

1441 catcaagcgg tcaattatca aacgcgttgc aacaacggta acgatgtccg tagcaccaca 1500

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 1560

1501 gtgcgagcag caaaccatac cgaagtaagt cgaggccaag ggttgctagt tccgctcaat 1560

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 1620

1561 gtactagggg gtacaacacg ttttttcgcc aatcgaggaa gccaggaggc tagcaacagt 1620

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 1680

1621 cttcattcaa ccggcgtcac aatagtgagt accaataccg tcgtgacgta ttaagagaat 1680

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 1740

1681 gacagtacgg taggcattct acgaaaagac actgaccact catgagttgg ttcagtaaga 1740

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 1800

1741 ctcttatcac atacgccgct ggctcaacga gaacgggccg cagttatgcc ctattatggc 1800

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 1860

1801 gcggtgtatc gtcttgaaat tttcacgagt agtaaccttt tgcaagaagc cccgcttttg 1860

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 1920

1861 agagttccta gaatggcgac aactctaggt caagctacat tgggtgagca cgtgggttga 1920

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 1980

1921 ctagaagtcg tagaaaatga aagtggtcgc aaagacccac tcgtttttgt ccttccgttt 1980

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 2040

1981 tacggcgttt tttcccttat tcccgctgtg cctttacaac ttatgagtat gagaaggaaa 2040

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 2100

2041 aagttataat aacttcgtaa atagtcccaa taacagagta ctcgcctatg tataaactta 2100

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

2101 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 2160

2101 cataaatctt tttatttgtt tatccccaag gcgcgtgtaa aggggctttt cacggtggac 2160

.......... .......... .......... .......... .......... ..........

AatII(2164) ........ .......... .......... .......... ..........

2161 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 2220

2161 tgcagattct ttggtaataa tagtactgta attggatatt tttatccgca tagtgctccg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cctttcgtct tcaagaatt. .......... .......... .......... .......... 2239

2221 ggaaagcaga agttcttaa. .......... .......... .......... .......... 2239

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-2239 Reference comment: pT7T3-18 Reference author: Gilbert W.; Reference title: "Obtained from VecBase 3.0"; Reference location: Unpublished (1991).