rd,rd,ÿID PT7T319U preliminary; circular DNA; SYN; 2883 BP. XX AC IG1129; XX DT 01-FEB-1993 (Rel. 7, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE E. coli phagemid vector pT7T3-19U - complete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RC pZf18R from pUC18 & pDM1, f1 RC pZf18U from pUC18 & pDM1, f1 RC pZf19R from pUC19 & pDM1, f1 RC pZf19U from pUC19 & pDM1, f1 RC pTZ18R from pZf18R & oligo, T7 promoter RC pTZ18U from pZf18U & oligo, T7 promoter RC pTZ19R from pZf19R & oligo, T7 promoter RC pTZ19U from pZf19U & oligo, T7 promoter RC pTP6 from pSP64-f1- & pTZ19R RC pC2A from pTZ18R & pP-450PBc2 RA Mead D.A., Szczesna-Skorupa E., Kemper B.; RT "Single-stranded DNA blue T7 promoter plasmids: a versatile RT tandem promoter system for cloning and protein engineering"; RL Protein Engineering 1:67-74(1986). XX CC NM (pT7T3-19U) CC CM (yes) CC NA (ds-DNA) CC TP (circular) CC ST () CC TY (phagemid) CC SP (Pharmacia) CC HO (E.coli NM522) CC CP () CC FN (cloning cDNA)(transcription)(mutagenesis) CC SE (color blue/white) CC PA (pUC18)(bacteriophage f1)(pTZ19U) CC BR (pT7T3-18U) CC OF () CC OR () XX FH Key Location/Qualifiers FH FT misc_feature 0..0 FT /note="1. pTZ19U 2863bp FT -> pT7T3-19U 2883bp" FT CDS complement(1..<448) FT /note="GEN E. coli beta-galactosidase gene (lacZ) FT alpha peptide" FT primer_bind 0..0 FT /note="PRI bacteriophage M13 universal" FT promoter 0..0 FT /note="PRO bacteriophage T3" FT misc_binding 0..0 FT /note="MCS unique HindIII-SphI-PstI-BspMI-SalI-XbaI- FT BamHI-SmaI-KpnI-SacI-EcoRI" FT promoter complement(0..0) FT /note="PRO bacteriophage T7" FT rep_origin 0..0 FT /note="ORI E. coli pMB1 (ColE1 and pBR322)" FT CDS complement(0..0) FT /note="ANT E. coli beta-lactamase gene (bla) FT ampicillin resistance gene (apr/amp)" FT misc_binding 1997..1997 FT /note="SIT unique ScaI" FT misc_binding 2114..2114 FT /note="SIT unique XmnI" FT rep_origin <2753..2883 FT /note="ORI bacteriophage f1 intergenic region" FT misc_binding 2753..2753 FT /note="SIT unique NaeI" XX SQ Sequence 2883 BP; 747 A; 710 C; 716 G; 710 T; 0 other; cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctatat aaattaaccc tcactaaagg gaataagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt ccctatagtg agtcgtatta aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa ttgtaaacgt taatgttttg ttaaatttcg cgttaaatat ttgttaaatc agcttatttt ttaaccagta agcagaaaat gacaaaaatc cttataaatc aaaagaatag accgagttag ttgtgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg taaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttg gaggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctactgggcg cgt // C pT7T3-19U ðÿÿÿ"Times New RomanÍÍÍÍrd,ÿCÍÍÍÍC œŒlacZ_aóÿÿÿ"Times New RomanèÍÍÍÍrd,†CÍÍÍÍC •Ž M13_pUC_fóÿÿÿ"Times New RomanèÍÍÍÍrd,CɆÍÍÍÍC ŽžŽM13_f20óÿÿÿ"Times New Romanè)ÍÍÍÍrd,†ÉÍÍÍÍC °¿ŽT3óÿÿÿ"Times New RomanèÍÍÍÍrd,ÉCÍÍÍÍC üŒT7óÿÿÿ"Times New RomanèÍÍÍÍrd,†CÍÍÍÍC +ŒM13_róÿÿÿ"Times New RomanèÍÍÍÍrd,CɆÍÍÍÍC *@Œ M13_pUC_róÿÿÿ"Times New RomanèÍÍÍÍrd,†ÉÍÍÍÍC NkŒlac_proóÿÿÿ"Times New RomanèÍÍÍÍrd,ÉCÍÍÍÍC   Œ pBR322_originóÿÿÿ"Times New RomanèÍÍÍÍrd,†CÍÍÍÍC ¦ Œ Orf frame 3óÿÿÿ"Times New Romanè(ÍÍÍÍrd,CɆÍÍÍÍC ¦ Œ Ampicillinóÿÿÿ"Times New RomanèÍÍÍÍrd,†ÉÍÍÍÍC , H Œ AmpR_promoteróÿÿÿ"Times New RomanèÍÍÍÍrd,ÉCÍÍÍÍC 2 Œ f1_originóÿÿÿ"Times New Romanè ÍÍÍÍrd,ÍÍÍÍÍÍÍÍfHindIII(195), PstI(211), SalI(213), XbaI(219), BamHI(225), SmaI(232), KpnI(238), SacI(244), EcoRI(246)óÿÿÿ"Times New Romanè0ŽC Úþÿÿ¶}Àløeø"A¨ ÍÍÍÍrd,ÍÍÍÍtestóÿÿÿ"Times New RomanÈC ¨w­ž¥¨+¨¥