Sequence of pT7T3-19

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Sequence of pT7T3-19

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Sequence of pT7T3-19 : 2238 BP; 572 A; 560 C; 533 G; 573 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aattctcatg tttgacagct tatcatcgac tccagtttct aattaaccct cactaaaggg 60

1 ttaagagtac aaactgtcga atagtagctg aggtcaaaga ttaattggga gtgatttccc 60

.......... .......... .......... ......... T3_promoter .........

EcoRI SacI KpnI,SmaI,BamHI96) XbaI(102) SalI(108)

61 agagaccata gatggaattc gagctcggta cccggggatc ctctagagtc gacctgcagg 120

61 tctctggtat ctaccttaag ctcgagccat gggcccctag gagatctcag ctggacgtcc 120

.......... .......... .......... .......... .......... ..........

HindIII(126) ........ .......... .......... .......... PvuII(172)

121 catgcaagct tgggtctccc tatagtgagt cgtattaatt tcgataagcc agctgcatta 180

121 gtacgttcga acccagaggg atatcactca gcataattaa agctattcgg tcgacgtaat 180

.......... ......... T7_promoter ......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 240

181 tacttagccg gttgcgcgcc cctctccgcc aaacgcataa cccgcgagaa ggcgaaggag 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 300

241 cgagtgactg agcgacgcga gccagcaagc cgacgccgct cgccatagtc gagtgagttt 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 360

301 ccgccattat gccaataggt gtcttagtcc cctattgcgt cctttcttgt acactcgttt 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 420

361 tccggtcgtt ttccggtcct tggcattttt ccggcgcaac gaccgcaaaa aggtatccga 420

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

421 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 480

421 ggcgggggga ctgctcgtag tgtttttagc tgcgagttca gtctccaccg ctttgggctg 480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 540

481 tcctgatatt tctatggtcc gcaaaggggg accttcgagg gagcacgcga gaggacaagg 540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 600

541 ctgggacggc gaatggccta tggacaggcg gaaagaggga agcccttcgc accgcgaaag 600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 660

601 agtatcgagt gcgacatcca tagagtcaag ccacatccag caagcgaggt tcgacccgac 660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 720

661 acacgtgctt ggggggcaag tcgggctggc gacgcggaat aggccattga tagcagaact 720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 780

721 caggttgggc cattctgtgc tgaatagcgg tgaccgtcgt cggtgaccat tgtcctaatc 780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 840

781 gtctcgctcc atacatccgc cacgatgtct caagaacttc accaccggat tgatgccgat 840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 900

841 gtgatcttcc tgtcataaac catagacgcg agacgacttc ggtcaatgga agcctttttc 900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 960

901 tcaaccatcg agaactaggc cgtttgtttg gtggcgacca tcgccaccaa aaaaacaaac 960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 1020

961 gttcgtcgtc taatgcgcgt ctttttttcc tagagttctt ctaggaaact agaaaagatg 1020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 1080

1021 ccccagactg cgagtcacct tgcttttgag tgcaattccc taaaaccagt actctaatag 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 1140

1081 tttttcctag aagtggatct aggaaaattt aatttttact tcaaaattta gttagatttc 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 1200

1141 atatatactc atttgaacca gactgtcaat ggttacgaat tagtcactcc gtggatagag 1200

.......... .......... .......... .... Orf frame 1 Ampicillin ....

.......... .......... .......... .......... .......... ..........

1201 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 1260

1201 tcgctagaca gataaagcaa gtaggtatca acggactgag gggcagcaca tctattgatg 1260

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 1320

1261 ctatgccctc ccgaatggta gaccggggtc acgacgttac tatggcgctc tgggtgcgag 1320

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 1380

1321 tggccgaggt ctaaatagtc gttatttggt cggtcggcct tcccggctcg cgtcttcacc 1380

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 1440

1381 aggacgttga aataggcgga ggtaggtcag ataattaaca acggcccttc gatctcattc 1440

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... FspI(1463) .......... .......... ..........

1441 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 1500

1441 atcaagcggt caattatcaa acgcgttgca acaacggtaa cgatgtccgt agcaccacag 1500

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 1560

1501 tgcgagcagc aaaccatacc gaagtaagtc gaggccaagg gttgctagtt ccgctcaatg 1560

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 1620

1561 tactaggggg tacaacacgt tttttcgcca atcgaggaag ccaggaggct agcaacagtc 1620

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 1680

1621 ttcattcaac cggcgtcaca atagtgagta ccaataccgt cgtgacgtat taagagaatg 1680

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 1740

1681 acagtacggt aggcattcta cgaaaagaca ctgaccactc atgagttggt tcagtaagac 1740

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 1800

1741 tcttatcaca tacgccgctg gctcaacgag aacgggccgc agttatgccc tattatggcg 1800

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 1860

1801 cggtgtatcg tcttgaaatt ttcacgagta gtaacctttt gcaagaagcc ccgcttttga 1860

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 1920

1861 gagttcctag aatggcgaca actctaggtc aagctacatt gggtgagcac gtgggttgac 1920

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 1980

1921 tagaagtcgt agaaaatgaa agtggtcgca aagacccact cgtttttgtc cttccgtttt 1980

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 2040

1981 acggcgtttt ttcccttatt cccgctgtgc ctttacaact tatgagtatg agaaggaaaa 2040

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 2100

2041 agttataata acttcgtaaa tagtcccaat aacagagtac tcgcctatgt ataaacttac 2100

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

2101 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 2160

2101 ataaatcttt ttatttgttt atccccaagg cgcgtgtaaa ggggcttttc acggtggact 2160

.......... .......... .......... .......... .......... ..........

AatII(2163) ........ .......... .......... .......... ..........

2161 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 2220

2161 gcagattctt tggtaataat agtactgtaa ttggatattt ttatccgcat agtgctccgg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ctttcgtctt caagaatt.. .......... .......... .......... .......... 2238

2221 gaaagcagaa gttcttaa.. .......... .......... .......... .......... 2238

.......... .......... .......... .......... .......... ..........