Sequence of pTAPKan1

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Sequence of pTAPKan1

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Sequence of pTAPKan1: 4711 BP; 1243 A; 1098 C; 1090 G; 1280 T; 0 other;

NdeI(6),BclI(9) KpnI(21),BamHI(26) NheI(35) ...... ..........

1 caccatatga tcaccggtac cctaggatcc agcgctagcg ctgtcgacgg tcttaaggcg 60

1 gtggtatact agtggccatg ggatcctagg tcgcgatcgc gacagctgcc agaattccgc 60

.......... .......... .......... ORF 1 .......... ..........

XmaI(62),SmaI(64) .. .......... .......... .......... ..........

61 cccgggttaa ttaaatctca cgaaaagaga agatggaaga agaacttcat tgctgtttct 120

61 gggcccaatt aatttagagt gcttttctct tctaccttct tcttgaagta acgacaaaga 120

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gctgctaaca gattcaagaa gatttcctct tctggtgctt tggactacga catttctaga 180

121 cgacgattgt ctaagttctt ctaaaggaga agaccacgaa acctgatgct gtaaagatct 180

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ccaactactg cctctgaaaa tttgtacttt caaggcgagt tgaagaccac tgcttctgaa 240

181 ggttgatgac ggagactttt aaacatgaaa gttccgctca acttctggtg acgaagactt 240

.......... .......... ORF 1 .......... .......... ..........

.......... .......... XhoI(266) .......... .......... ..........

241 aacttgtact tccaaggtga gttactcgag ggtgaattga agactgctgc tttggctcaa 300

241 ttgaacatga aggttccact caatgagctc ccacttaact tctgacgacg aaaccgagtt 300

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cacgacgaag ctgttgataa taagttcaat aaggaacagc aaaacgcatt ctacgagatt 360

301 gtgctgcttc gacaactatt attcaagtta ttccttgtcg ttttgcgtaa gatgctctaa 360

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ttgcatttgc caaatttgaa cgaggagcaa cgtaacgctt ttattcaatc cttgaaggat 420

361 aacgtaaacg gtttaaactt gctcctcgtt gcattgcgaa aataagttag gaacttccta 420

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gacccatccc aatctgccaa cttgttagct gaagccaaga agttaaacga cgctcaagcc 480

421 ctgggtaggg ttagacggtt gaacaatcga cttcggttct tcaatttgct gcgagttcgg 480

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ccaaaagttg acaataagtt taacaaggaa caacaaaacg ccttttacga aattttgcac 540

481 ggttttcaac tgttattcaa attgttcctt gttgttttgc ggaaaatgct ttaaaacgtg 540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ttgccaaact tgaacgaaga acaaagaaac gctttcattc aatctttgaa ggacgaccca 600

541 aacggtttga acttgcttct tgtttctttg cgaaagtaag ttagaaactt cctgctgggt 600

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tctcaatctg ctaatttgtt ggctgaagct aagaagttga acggtgctca agctccaaag 660

601 agagttagac gattaaacaa ccgacttcga ttcttcaact tgccacgagt tcgaggtttc 660

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gttgacgcta actctgctgg taagtctact tgaggcgcgc cacttctaaa taagcgaatt 720

661 caactgcgat tgagacgacc attcagatga actccgcgcg gtgaagattt attcgcttaa 720

.......... ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat 780

721 agaatactaa atactaaaaa taataattta ttcaatattt tttttattca catatgttta 780

.......... .......... .......... .......... ...... pHybLex_rev_primer

.......... .......... .......... .......... .......... ..........

781 tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt 840

781 aaatttcact gagaatccaa aattttgctt ttaagaataa gaactcattg agaaaggaca 840

pHybLex_rev_primer .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg 900

841 tccagtccaa cgaaagagtc catatcatac tccagcgaga ataactggtg tggagatggc 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... BglII(932) .......... ..........

901 gcagatccgc tagggataac agggtaatat agatctgttt agcttgcctc gtccccgccg 960

901 cgtctaggcg atccctattg tcccattata tctagacaaa tcgaacggag caggggcggc 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc 1020

961 ccagtgggcc ggtcgctgta cctccgggtc ttatgggagg aactgtcaga actgcacgcg 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa 1080

1021 tcgagtcccc gtactacact gacagcgggc atgtaaatcg ggtatgtagg ggtacatatt 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg 1140

1081 agtaaacgta ggtatgtaaa actaccggcg tgccgcgctt cgtttttaat gccgaggagc 1140

.......... .......... .......... .......... .......... ..........

PstI(1146) .......... .......... .......... .......... ..........

1141 ctgcagacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc 1200

1141 gacgtctgga cgctcgtccc tttgcgaggg gagtgtctgc gcaacttaac aggggtgcgg 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata 1260

1201 cgcggggaca tctctttata ttttccaatc ctaaacggtg actccaagaa gaaagtatat 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca taaacaacca 1320

1261 gaaggaaaat tttagaacga tcctatgtca agagtgtagt gtaggcttgt atttgttggt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tgggtaagga aaagactcac gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt 1380

1321 acccattcct tttctgagtg caaagctccg gcgctaattt aaggttgtac ctacgactaa 1380

.......... .......... kanMX6 .......... .......... ..........

.......... .......... NruI(1403) .......... .......... ClaI(1437)

1381 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat 1440

1381 atatacccat atttacccga gcgctattac agcccgttag tccacgctgt tagatagcta 1440

.......... .......... kanMX6 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca 1500

1441 acataccctt cgggctacgc ggtctcaaca aagactttgt accgtttcca tcgcaacggt 1500

.......... .......... kanMX6 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga 1560

1501 tactacaatg tctactctac cagtctgatt tgaccgactg ccttaaatac ggagaaggct 1560

.......... .......... kanMX6 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg 1620

1561 ggtagttcgt aaaataggca tgaggactac tacgtaccaa tgagtggtga cgctaggggc 1620

.......... .......... kanMX6 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg 1680

1621 cgttttgtcg taaggtccat aatcttctta taggactaag tccactttta taacaactac 1680

.......... .......... kanMX6 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca 1740

1681 gcgaccgtca caaggacgcg gccaacgtaa gctaaggaca aacattaaca ggaaaattgt 1740

.......... .......... kanMX6 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg 1800

1741 cgctagcgca taaagcagag cgagtccgcg ttagtgctta cttattgcca aaccaactac 1800

.......... .......... kanMX6 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc 1860

1801 gctcactaaa actactgctc gcattaccga ccggacaact tgttcagacc tttctttacg 1860

.......... .......... kanMX6 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ataagctttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata 1920

1861 tattcgaaaa cggtaagagt ggcctaagtc agcagtgagt accactaaag agtgaactat 1920

.......... .......... kanMX6 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg 1980

1921 tggaataaaa actgctcccc tttaattatc caacataact acaacctgct cagccttagc 1980

.......... .......... kanMX6 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat 2040

1981 gtctggctat ggtcctagaa cggtaggata ccttgacgga gccactcaaa agaggaagta 2040

.......... .......... kanMX6 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt 2100

2041 atgtctttgc cgaaaaagtt tttataccat aactattagg actatactta tttaacgtca 2100

.......... .......... kanMX6 .......... .......... ..........

.......... .......... .......... ScaI(2135) .......... ..........

2101 ttcatttgat gctcgatgag tttttctaat cagtactgac aataaaaaga ttcttgtttt 2160

2101 aagtaaacta cgagctactc aaaaagatta gtcatgactg ttatttttct aagaacaaaa 2160

.......... kanMX6 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt 2220

2161 gttcttgaac agtaaacata tcaaaaaaat ataacatcaa caagataaaa ttagtttaca 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc 2280

2221 atcgcactaa atataaaaaa aagcggagct gtagtagacg ggtctacgct tcaattcacg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat 2340

2281 cgtctttcat tatagtacgc agttagcata cacttacgac cagcgatatg acgacagcta 2340

.......... .......... .......... .......... .......... ..........

.......... .... PmeI(2367) SacI(2376),EcoRI(2378) ClaI(2385) ....

2341 tcgatactaa cgccgccatc cagtttaaac gagctcgaat tcatcgatga tatcagatcc 2400

2341 agctatgatt gcggcggtag gtcaaatttg ctcgagctta agtagctact atagtctagg 2400

.......... .......... .......... .......... .......... ..........

SpeI(2402) .......... .......... .......... .......... ..........

2401 actagtggcc tatgcggccg cggatctgcc ggtctcccta tagtgagtcg tattaatttc 2460

2401 tgatcaccgg atacgccggc gcctagacgg ccagagggat atcactcagc ataattaaag 2460

.......... .......... .......... ......... T7_promoter .........

.......... HpaI(2474) .......... .......... .......... ..........

2461 gataagccag gttaacctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 2520

2461 ctattcggtc caattggacg taattactta gccggttgcg cgcccctctc cgccaaacgc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 2580

2521 ataacccgcg agaaggcgaa ggagcgagtg actgagcgac gcgagccagc aagccgacgc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 2640

2581 cgctcgccat agtcgagtga gtttccgcca ttatgccaat aggtgtctta gtcccctatt 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 2700

2641 gcgtcctttc ttgtacactc gttttccggt cgttttccgg tccttggcat ttttccggcg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 2760

2701 caacgaccgc aaaaaggtat ccgaggcggg gggactgctc gtagtgtttt tagctgcgag 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 2820

2761 ttcagtctcc accgctttgg gctgtcctga tatttctatg gtccgcaaag ggggaccttc 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 2880

2821 gagggagcac gcgagaggac aaggctggga cggcgaatgg cctatggaca ggcggaaaga 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta 2940

2881 gggaagccct tcgcaccgcg aaagagttac gagtgcgaca tccatagagt caagccacat 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 3000

2941 ccagcaagcg aggttcgacc cgacacacgt gcttgggggg caagtcgggc tggcgacgcg 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 3060

3001 gaataggcca ttgatagcag aactcaggtt gggccattct gtgctgaata gcggtgaccg 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 3120

3061 tcgtcggtga ccattgtcct aatcgtctcg ctccatacat ccgccacgat gtctcaagaa 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 3180

3121 cttcaccacc ggattgatgc cgatgtgatc ttcctgtcat aaaccataga cgcgagacga 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 3240

3181 cttcggtcaa tggaagcctt tttctcaacc atcgagaact aggccgtttg tttggtggcg 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 3300

3241 accatcgcca ccaaaaaaac aaacgttcgt cgtctaatgc gcgtcttttt ttcctagagt 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 3360

3301 tcttctagga aactagaaaa gatgccccag actgcgagtc accttgcttt tgagtgcaat 3360

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 3420

3361 tccctaaaac cagtactcta atagtttttc ctagaagtgg atctaggaaa atttaatttt 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 3480

3421 tacttcaaaa tttagttaga tttcatatat actcatttga accagactgt caatggttac 3480

.......... .......... .......... .......... .......... bla ORF 1

.......... .......... .......... .......... .......... ..........

3481 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 3540

3481 gaattagtca ctccgtggat agagtcgcta gacagataaa gcaagtaggt atcaacggac 3540

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 3600

3541 tgaggggcag cacatctatt gatgctatgc cctcccgaat ggtagaccgg ggtcacgacg 3600

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 3660

3601 ttactatggc gctctgggtg cgagtggccg aggtctaaat agtcgttatt tggtcggtcg 3660

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 3720

3661 gccttcccgg ctcgcgtctt caccaggacg ttgaaatagg cggaggtagg tcagataatt 3720

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 3780

3721 aacaacggcc cttcgatctc attcatcaag cggtcaatta tcaaacgcgt tgcaacaacg 3780

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 3840

3781 gtaacgatgt ccgtagcacc acagtgcgag cagcaaacca taccgaagta agtcgaggcc 3840

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 3900

3841 aagggttgct agttccgctc aatgtactag ggggtacaac acgttttttc gccaatcgag 3900

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 3960

3901 gaagccagga ggctagcaac agtcttcatt caaccggcgt cacaatagtg agtaccaata 3960

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 4020

3961 ccgtcgtgac gtattaagag aatgacagta cggtaggcat tctacgaaaa gacactgacc 4020

.......... .......... bla ORF 1 .......... .......... ..........

ScaI(4026) .......... .......... .......... .......... ..........

4021 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 4080

4021 actcatgagt tggttcagta agactcttat cacatacgcc gctggctcaa cgagaacggg 4080

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 4140

4081 ccgcagttat gccctattat ggcgcggtgt atcgtcttga aattttcacg agtagtaacc 4140

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 4200

4141 ttttgcaaga agccccgctt ttgagagttc ctagaatggc gacaactcta ggtcaagcta 4200

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 4260

4201 cattgggtga gcacgtgggt tgactagaag tcgtagaaaa tgaaagtggt cgcaaagacc 4260

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 4320

4261 cactcgtttt tgtccttccg ttttacggcg ttttttccct tattcccgct gtgcctttac 4320

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 4380

4321 aacttatgag tatgagaagg aaaaagttat aataacttcg taaatagtcc caataacaga 4380

bla ORF 1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 4440

4381 gtactcgcct atgtataaac ttacataaat ctttttattt gtttatcccc aaggcgcgtg 4440

........ AmpR_promoter ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 4500

4441 taaaggggct tttcacggtg gactgcagat tctttggtaa taatagtact gtaattggat 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 4560

4501 atttttatcc gcatagtgct ccgggaaagc agagcgcgca aagccactac tgccactttt 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 4620

4561 ggagactgtg tacgtcgagg gcctctgcca gtgtcgaaca gacattcgcc tacggccctc 4620

........ pGEX_3_primer ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 4680

4621 gtctgttcgg gcagtcccgc gcagtcgccc acaaccgccc acagccccga ccgaattgat 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tgcggcatca gagcagattg tactgagagt g......... .......... .......... 4711

4681 acgccgtagt ctcgtctaac atgactctca c......... .......... .......... 4711

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-4711 Reference author: Spirek M., Gregan J., Chen Z.; Reference title: "Yeast tagging vector pTAPKan1"; Reference location: Unpublished.
Reference number: [2] Reference positions: 1-4711 Reference author: Spirek M., Gregan J., Chen Z.; Reference title: ; Reference location: Submitted (07-OCT-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Department of Chromosome Biology, University of Vienna, Viehmarktgasse 2a, Reference location: Wien 1030, Austria