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    Plasmid map of pTiger

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    pTiger
    Backbone manufacturer: Nolan Lab
    Type of vector: Lentiviral
    Backbone size (bp): 7164
    5' Sequencing primer: n/a
    3' Sequencing primer: M13 reverse primer
    Bacteria resistance: Ampicillin
    High or low copy: Don't Know
    Grow in standard E. coli @ 37C: Yes
    Plasmid Provided In: DH5a
    Principal Investigator: Garry Nolan
    Comments: This is a transfer vector (highest titer) from Nolan lab's FELIX vector system. The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB.



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