Vector backbone: pTiger
Backbone manufacturer: Nolan Lab
Type of vector: Lentiviral
Backbone size (bp): 7164
5' Sequencing primer: n/a
3' Sequencing primer: M13 reverse primer
Bacteria resistance: Ampicillin
High or low copy: Don't Know
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Garry Nolan
Comments: This is a transfer vector (highest titer) from Nolan lab's FELIX vector system. The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB.