Sequence of pTiger

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Sequence of pTiger

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Sequence of pTiger : 7164 BP; 1883 A; 1750 C; 1604 G; 1927 T; 0 other;

.......... .......... .......... .......... .......... PvuII(55)

1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60

1 tcgcgggtta tgcgtttggc ggagaggggc gcgcaaccgg ctaagtaatt acgtcgaccg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120

61 tgctgtccaa agggctgacc tttcgcccgt cactcgcgtt gcgttaatta cactcaatcg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180

121 agtgagtaat ccgtggggtc cgaaatgtga aatacgaagg ccgagcatac aacacacctt 180

.......... .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 240

181 aacactcgcc tattgttaaa gtgtgtcctt tgtcgatact ggtactaatg cggttcgaac 240

.......... M13_pUC_r .......... M13_r .......... ..........

.......... .......... .......... .......... .......... ..........

241 catgcctgca gtgcgaagtt ctcggcccgg attccgagac ctcacaggta aaaaagatta 300

241 gtacggacgt cacgcttcaa gagccgggcc taaggctctg gagtgtccat tttttctaat 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cacagatact cgacagggtt caatctcaaa tatttattgt atctgtggga gcctcaaggg 360

301 gtgtctatga gctgtcccaa gttagagttt ataaataaca tagacaccct cggagttccc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 agaactcaaa agtcctcaac aaagagactc ctcgaagttt cacaaagcac tggttaaggc 420

361 tcttgagttt tcaggagttg tttctctgag gagcttcaaa gtgtttcgtg accaattccg 420

.......... .......... .......... .......... ORF1 ..........

.......... .......... .......... .......... .......... ..........

421 cttcagggtt ccaatactca tcccagtcca cccttttttc ttttttagga gggccccccc 480

421 gaagtcccaa ggttatgagt agggtcaggt gggaaaaaag aaaaaatcct cccggggggg 480

.......... ......... ORF1 U3PPT U3PPT ......... ..........

.......... .......... .......... .......... .......... ..........

481 tcgaggtcga cggtatcgat caggcgggga ggcggcccaa agggagatcc gactcgtctg 540

481 agctccagct gccatagcta gtccgcccct ccgccgggtt tccctctagg ctgagcagac 540

pBluesKS_pri ........ ORF1 .......... WPRE ..........

.......... .......... .......... .......... SacII(590) ..........

541 agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag 600

541 tcccgcttcc gcttctgcgc cttctccggc gtctcggccg tcgtccggcg cccttccttc 600

.......... .......... ORF1 WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt 660

601 caggcgacct aactcccggc ttccctgcat cgtcttcctg cagggcgcgt cttaggtcca 660

.......... .......... ORF1 WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ggcaacacag gcgagcagcc aaggaaagga cgatgatttc cccgacaaca ccacggaatt 720

661 ccgttgtgtc cgctcgtcgg ttcctttcct gctactaaag gggctgttgt ggtgccttaa 720

.......... .......... ORF1 WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc 780

721 cagtcacggg ttgtcggctc ggggacaggt cgtcgcccgt tccgtccgcc gctactcaag 780

.......... .......... ORF1 WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc 840

781 gcggcaccgt tatccctccc cctttcgctt tcagggcctt tcctcgactg tccaccaccg 840

.......... .......... ORF1 WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct 900

841 ttacggggtt ggtcaccccc aacgcagtcg tttgtgtcac gtgtggtgcg gtgcaacgga 900

.......... .......... ORF1 WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa 960

901 ctgttgcccg gtgttgagga gtatttctct gtcgttggtc ctaaatatgt tcctcctctt 960

.......... .......... ORF1 WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca 1020

961 ttactttcgg tatgcccttc gttatcgtac tatgtttccg taatttcgtc gcataggtgt 1020

.......... .......... ORF1 WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tagcgtaaaa ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc 1080

1021 atcgcatttt cctcgttgta tcaattctta tggtcagtta gaaagtgttt aaaacattag 1080

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cagaggttga ttatcgataa gcttgatgaa ggctttcttc tttcttcttc ttctttgtct 1140

1081 gtctccaact aatagctatt cgaactactt ccgaaagaag aaagaagaag aagaaacaga 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... BstBI(1185) .........

1141 tttcctttta ccttacaata catactttat tagtttgatt tgattcgaaa tggattcata 1200

1141 aaaggaaaat ggaatgttat gtatgaaata atcaaactaa actaagcttt acctaagtat 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tgacatacct tcctcaaagg gaagaactca gcacagtatc tcccttataa aataagtctg 1260

1201 actgtatgga aggagtttcc cttcttgagt cgtgtcatag agggaatatt ttattcagac 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 agatacttca tcattcctcc tctttttcag acatgccaca ttgcctacca tttctcctca 1320

1261 tctatgaagt agtaaggagg agaaaaagtc tgtacggtgt aacggatggt aaagaggagt 1320

.......... .......... .......... Env_FIV .......... ..........

.......... .......... .......... .......... .......... ..........

1321 attccatttg tggttgtatt tcttctcctt ctacttcagg cattgcaatt actgtgtatc 1380

1321 taaggtaaac accaacataa agaagaggaa gatgaagtcc gtaacgttaa tgacacatag 1380

.......... .......... Env_FIV .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ctagtatctt gtggatacaa tttcttatac aatcaaccaa tgtaggtaaa cataaaatca 1440

1381 gatcatagaa cacctatgtt aaagaatatg ttagttggtt acatccattt gtattttagt 1440

.......... .......... Env_FIV .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ataataacac tcctaatcct attcccaaga tacctcccaa tagtcccttt aaatattgtg 1500

1441 tattattgtg aggattagga taagggttct atggagggtt atcagggaaa tttataacac 1500

.......... .......... Env_FIV .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gaatatttcc tatccatcct acccaatctt cccacttttg taattgttgt atccctttct 1560

1501 cttataaagg ataggtagga tgggttagaa gggtgaaaac attaacaaca tagggaaaga 1560

.......... .......... Env_FIV .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tcccttgtac attattttgt tctatgtcca ttattatttc ataaaacttt tgttgtaaat 1620

1561 agggaacatg taataaaaca agatacaggt aataataaag tattttgaaa acaacattta 1620

.......... .......... Env_FIV .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cttttgtttg gttataccat tcccccaaag ttatatttcc atgattccat attgtttgat 1680

1621 gaaaacaaac caatatggta agggggtttc aatataaagg tactaaggta taacaaacta 1680

.......... .......... Env_FIV .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ttatagtcat attatacctt gtccacaact caggagggat tttgcagaaa aattgatttt 1740

1681 aatatcagta taatatggaa caggtgttga gtcctcccta aaacgtcttt ttaactaaaa 1740

.......... .......... Env_FIV .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gattacatcc taattcttgc atagcgaaag ctgtatacaa aaatttttcc atagcttcta 1800

1741 ctaatgtagg attaagaacg tatcgctttc gacatatgtt tttaaaaagg tatcgaagat 1800

.......... .......... Env_FIV .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cttttaatcc tattactagc ttctattagg gcgcactcga gcggccgcca gtgtgctgga 1860

1801 gaaaattagg ataatgatcg aagataatcc cgcgtgagct cgccggcggt cacacgacct 1860

Env_FIV .......... .......... .......... .......... ..........

PmlI(1870) .......... .......... .......... .......... ..........

1861 attcaggcac gtgcaatttt tgtggtattg cagaaaaata atcttgtatt cttaaggatt 1920

1861 taagtccgtg cacgttaaaa acaccataac gtctttttat tagaacataa gaattcctaa 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cttgttgtgc taataattca taaggggcca tccctcctat cctcctaggc ctgaattctg 1980

1921 gaacaacacg attattaagt attccccggt agggaggata ggaggatccg gacttaagac 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cagatatcca tcacactggc ggccggccgc cactgtgctg gatatctgca gaattccacc 2040

1981 gtctataggt agtgtgaccg ccggccggcg gtgacacgac ctatagacgt cttaaggtgg 2040

.......... .......... .......... .......... .......... ..........

......... BamHI(2054) ......... .......... NheI(2088) ..........

2041 acactggact agtggatccg agctcggtac caagcttaag tttaaacgct agccagcttg 2100

2041 tgtgacctga tcacctaggc tcgagccatg gttcgaattc aaatttgcga tcggtcgaac 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ggtctcccta tagtgagtcg tattaatttc gataagccag taagcagtgg gttctctagt 2160

2101 ccagagggat atcactcagc ataattaaag ctattcggtc attcgtcacc caagagatca 2160

.......... T7 .......... ......... CMV_imm_pro .........

.......... .......... .......... .......... .......... ..........

2161 tagccagaga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt 2220

2161 atcggtctct cgagacgaat atatctggag ggtggcatgt gcggatggcg g gtaaacgca 2220

.......... ......... CMV_imm_pro ......... CMV_f_pri ..........

.......... .......... .......... .......... .......... ..........

2221 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa 2280

2221 gttaccccgc ctcaacaatg ctgtaaaacc tttcagggca actaaaacca cggttttgtt 2280

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc 2340

2281 tgagggtaac tgcagttacc ccacctctga acctttaggg gcactcagtt tggcgatagg 2340

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta 2400

2341 tgcgggtaac tacatgacgg ttttggcgta gtggtaccat tatcgctact gattatgcat 2400

.......... ......... CMV_imm_pro ......... .......... CAG_enh

.......... .......... .......... .......... .......... ..........

2401 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg 2460

2401 ctacatgacg gttcatcctt tcagggtatt ccagtacatg acccgtatta cggtccgccc 2460

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac 2520

2461 ggtaaatggc agtaactgca gttatccccc gcatgaaccg tatactatgt gaactacatg 2520

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgccaagtgg gcagtttacc gtaaatagtc cacccattga cgtcaatgga aagtccctat 2580

2521 acggttcacc cgtcaaatgg catttatcag gtgggtaact gcagttacct ttcagggata 2580

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg 2640

2581 accgcaatga tacccttgta tgcagtaata actgcagtta cccgccccca gcaacccgcc 2640

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg 2700

2641 agtcggtccg cccggtaaat ggcattcaat acattgcgcc ttgaggtata tacccgatac 2700

......... CAG_enh CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aactaatgac cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt 2760

2701 ttgattactg gggcattaac taatgataat tattgatcag ttattagtta cagttgcgca 2760

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 atatctggcc cgtacatcgc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc 2820

2761 tatagaccgg gcatgtagcg ccatgggtta agcgggatat cactcagcat aatgcgcgcg 2820

.......... .......... .......... .......... T7 ..........

.......... .......... .......... .......... .......... ..........

2821 cgtcatattt aaaagtcctg ctaccgcaaa gacttttaat gtcacaatta ctccttccat 2880

2821 gcagtataaa ttttcaggac gatggcgttt ctgaaaatta cagtgttaat gaggaaggta 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cttttgattt ttaaaatttg gtccattatc tgtttgtaat tcagtaacat tatgagcact 2940

2881 gaaaactaaa aattttaaac caggtaatag acaaacatta agtcattgta atactcgtga 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 caacaattgt aagacagctt taactgtaca gtcagcagtt tcttgagaaa ttatttgtgc 3000

2941 gttgttaaca ttctgtcgaa attgacatgt cagtcgtcaa agaactcttt aataaacacg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ccatatatat cctgattcca catgtataca attgccatat caatttcttt gctagatcca 3060

3001 ggtatatata ggactaaggt gtacatatgt taacggtata gttaaagaaa cgatctaggt 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aatttttctc ttctttcttg taaatcgcaa ataaccaacc ttagttgatc taaagtctct 3120

3061 ttaaaaagag aagaaagaac atttagcgtt tattggttgg aatcaactag atttcagaga 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggtatatcac caggttctcg tcctgtagat acattagcca ttctaatggc ccatctgaaa 3180

3121 ccatatagtg gtccaagagc aggacatcta tgtaatcggt aagattaccg ggtagacttt 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttcccttctc caaatttttt actcttcccc cctactccta cagcaacatt actacatctc 3240

3181 aagggaagag gtttaaaaaa tgagaagggg ggatgaggat gtcgttgtaa tgatgtagag 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttaatggcca ttttccaatc tcgcccctgt ccattcccca tgttgctgta gaatctctcc 3300

3241 aattaccggt aaaaggttag agcggggaca ggtaaggggt acaacgacat cttagagagg 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 taccttgttg actgtccctc ggcgaatctc ctggcttgaa ggtccgcgaa gagtcaccag 3360

3301 atggaacaac tgacagggag ccgcttagag gaccgaactt ccaggcgctt ctcagtggtc 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atgtaattta tctgggcctt taaacaatga cttgattatg agctcgagtc cgcttcacta 3420

3361 tacattaaat agacccggaa atttgttact gaactaatac tcgagctcag gcgaagtgat 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gagatactca ctgttagcag cgtctgctac tgcttcccta tttaggtcag caggagttct 3480

3421 ctctatgagt gacaatcgtc gcagacgatg acgaagggat aaatccagtc gtcctcaaga 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gcttaacagc tttctattgc tctagcttca cttcctcaat cactctcaat caagtccctg 3540

3481 cgaattgtcg aaagataacg agatcgaagt gaaggagtta gtgagagtta gttcagggac 3540

.......... .......... .......... .......... .......... ..........

NarI(3546) .......... .......... .......... .......... ..........

3541 ttcgggcgcc aactgcgaag ttctcggccc ggattccgag acctcacagg taaaaaagat 3600

3541 aagcccgcgg ttgacgcttc aagagccggg cctaaggctc tggagtgtcc attttttcta 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tacacagata ctcgacaggg ttcaatctca aatatttatt gtatctgtgg gagcctcaag 3660

3601 atgtgtctat gagctgtccc aagttagagt ttataaataa catagacacc ctcggagttc 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ggagaactca aaagtcctca acaaagagac tcctcgaagt ttcacagagc tctgcttata 3720

3661 cctcttgagt tttcaggagt tgtttctctg aggagcttca aagtgtctcg agacgaatat 3720

.......... .......... .......... .......... ......... CMV_imm_pro

.......... .......... .......... .......... .......... ..........

3721 tagacctccc accgtacacg cctaccgccc atttgcgtca acggggcggg gttattacga 3780

3721 atctggaggg tggcatgtgc ggatggcggg taaacgcagt tgccccgccc caataatgct 3780

.......... .... CMV_imm_pro CMV_f_pri .... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg 3840

3781 gtaaaacctt tcagggcaac taaaaccacg gttttgtttg agggtaactg cagttacccc 3840

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattggt gtactgccaa 3900

3841 acctctgaac ctttaggggc actcagtttg gcgataggtg cgggtaacca catgacggtt 3900

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag 3960

3901 ttggcgtagt ggtaccatta tcgctactga ttatgcatct acatgacggt tcatcctttc 3960

.......... ......... CMV_imm_pro ......... CAG_enh ..........

.......... .......... .......... .......... .......... ..........

3961 tcccgtaagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca 4020

3961 agggcattcc agtacatgac ccgtattacg gtccgcccgg taaatggcag taactgcagt 4020

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 atagggggcg gacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt 4080

4021 tatcccccgc ctgaaccgta tactatgtga actacatgac ggttcacccg tcaaatggca 4080

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac 4140

4081 tttatgaggt gggtaactgc agttaccttt cagggataac cgcaatgata cccttgtatg 4140

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc 4200

4141 cagtaataac tgcagttacc cgcccccagc aacccgccag tcggtccgcc cggtaaatgg 4200

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat 4260

4201 cattcaatac attgcgcctt gaggtatata cccgatactt gattactggg gcattaacta 4260

CAG_enh ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tactattaat aactagtcaa taatcaatgc caacatggcg gtcatattgg acatgagcca 4320

4261 atgataatta ttgatcagtt attagttacg gttgtaccgc cagtataacc tgtactcggt 4320

CMV_imm_pro ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 atataaatgt acatattatg atatagatac aacgtatgca atggccaata gccaatattg 4380

4321 tatatttaca tgtataatac tatatctatg ttgcatacgt taccggttat cggttataac 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... BglII(4429) .........

4381 atttatgcta tataaccaat gaataatatg gctaatggcc aatattgaag atctgtcgag 4440

4381 taaatacgat atattggtta cttattatac cgattaccgg ttataacttc tagacagctc 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ccatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 4500

4441 ggtacactcg ttttccggtc gttttccggt ccttggcatt tttccggcgc aacgaccgca 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 4560

4501 aaaaggtatc cgaggcgggg ggactgctcg tagtgttttt agctgcgagt tcagtctcca 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 4620

4561 ccgctttggg ctgtcctgat atttctatgg tccgcaaagg gggaccttcg agggagcacg 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gctctcctgt tccgaccctg ccgcttaccg gctttccccg tcaagctcta aatcgggggc 4680

4621 cgagaggaca aggctgggac ggcgaatggc cgaaaggggc agttcgagat ttagcccccg 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg 4740

4681 agggaaatcc caaggctaaa tcacgaaatg ccgtggagct ggggtttttt gaactaaacc 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 4800

4741 cactaccaag tgcatcaccc ggtagcggga ctatctgcca aaaagcggga aactgcaacc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 4860

4801 tcaggtgcaa gaaattatca cctgagaaca aggtttgacc ttgttgtgag ttgggataga 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 4920

4861 gcccgataag aaaactaaat attccctaaa acggctaaag ccggataacc aattttttac 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat 4980

4921 tcgactaaat tgtttttaaa ttgcgcttaa aattgtttta taattgcaaa tgttaaaata 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc 5040

4981 ccacgtgaga gtcatgttag acgagactac ggcgtatcaa ttcggtcggg gctgtgggcg 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag 5100

5041 gttgtgggcg actgcgcggg actgcccgaa cagacgaggg ccgtaggcga atgtctgttc 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg 5160

5101 gacactggca gaggccctcg acgtacacag tctccaaaag tggcagtagt ggctttgcgc 5160

.......... .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg 5220

5161 gctctgcttt cccggagcac tatgcggata aaaatatcca attacagtac tattattacc 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 5280

5221 aaagaatctg cagtccaccg tgaaaagccc ctttacacgc gccttgggga taaacaaata 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 5340

5281 aaaagattta tgtaagttta tacataggcg agtactctgt tattgggact atttacgaag 5340

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 5400

5341 ttattataac tttttccttc tcatactcat aagttgtaaa ggcacagcgg gaataaggga 5400

.......... .......... .......... ....... ORF3 Ampicillin .......

.......... .......... .......... .......... .......... ..........

5401 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 5460

5401 aaaaacgccg taaaacggaa ggacaaaaac gagtgggtct ttgcgaccac tttcattttc 5460

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 5520

5461 tacgacttct agtcaaccca cgtgctcacc caatgtagct tgacctagag ttgtcgccat 5520

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 5580

5521 tctaggaact ctcaaaagcg gggcttcttg caaaaggtta ctactcgtga aaatttcaag 5580

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 5640

5581 acgatacacc gcgccataat agggcataac tgcggcccgt tctcgttgag ccagcggcgt 5640

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 5700

5641 atgtgataag agtcttactg aaccaactca tgagtggtca gtgtcttttc gtagaatgcc 5700

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 5760

5701 taccgtactg tcattctctt aatacgtcac gacggtattg gtactcacta ttgtgacgcc 5760

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 5820

5761 ggttgaatga agactgttgc tagcctcctg gcttcctcga ttggcgaaaa aacgtgttgt 5820

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 5880

5821 accccctagt acattgagcg gaactagcaa cccttggcct cgacttactt cggtatggtt 5880

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... FspI(5928) ..........

5881 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 5940

5881 tgctgctcgc actgtggtgc tacggacatc gttaccgttg ttgcaacgcg tttgataatt 5940

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 6000

5941 gaccgcttga tgaatgagat cgaagggccg ttgttaatta tctgacctac ctccgcctat 6000

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 6060

6001 ttcaacgtcc tggtgaagac gcgagccggg aaggccgacc gaccaaataa cgactattta 6060

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 6120

6061 gacctcggcc actcgcaccc agagcgccat agtaacgtcg tgaccccggt ctaccattcg 6120

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 6180

6121 ggagggcata gcatcaatag atgtgctgcc cctcagtccg ttgataccta cttgctttat 6180

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 6240

6181 ctgtctagcg actctatcca cggagtgact aattcgtaac cattgacagt ctggttcaaa 6240

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 6300

6241 tgagtatata tgaaatctaa ctaaattttg aagtaaaaat taaattttcc tagatccact 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 6360

6301 tctaggaaaa actattagag tactggtttt agggaattgc actcaaaagc aaggtgactc 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 6420

6361 gcagtctggg gcatcttttc tagtttccta gaagaactct aggaaaaaaa gacgcgcatt 6420

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

6421 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 6480

6421 agacgacgaa cgtttgtttt tttggtggcg atggtcgcca ccaaacaaac ggcctagttc 6480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6481 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 6540

6481 tcgatggttg agaaaaaggc ttccattgac cgaagtcgtc tcgcgtctat ggtttatgac 6540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6541 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 6600

6541 aggaagatca catcggcatc aatccggtgg tgaagttctt gagacatcgt ggcggatgta 6600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6601 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 6660

6601 tggagcgaga cgattaggac aatggtcacc gacgacggtc accgctattc agcacagaat 6660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6661 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 6720

6661 ggcccaacct gagttctgct atcaatggcc tattccgcgt cgccagcccg acttgccccc 6720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6721 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 6780

6721 caagcacgtg tgtcgggtcg aacctcgctt gctggatgtg gcttgactct atggatgtcg 6780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6781 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 6840

6781 cactcgatac tctttcgcgg tgcgaagggc ttccctcttt ccgcctgtcc ataggccatt 6840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 6900

6841 cgccgtccca gccttgtcct ctcgcgtgct ccctcgaagg tccccctttg cggaccatag 6900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6901 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 6960

6901 aaatatcagg acagcccaaa gcggtggaga ctgaactcgc agctaaaaac actacgagca 6960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6961 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 7020

6961 gtccccccgc ctcggatacc tttttgcggt cgttgcgccg gaaaaatgcc aaggaccgga 7020

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 7080

7021 aaacgaccgg aaaacgagtg tacaagaaag gacgcaatag gggactaaga cacctattgg 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 7140

7081 cataatggcg gaaactcact cgactatggc gagcggcgtc ggcttgctgg ctcgcgtcgc 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 agtcagtgag cgaggaagcg gaag...... .......... .......... .......... 7164

7141 tcagtcactc gctccttcgc cttc...... .......... .......... .......... 7164

.......... .......... .......... .......... .......... ..........