*]*]#LOCUS pTriEx-3 5082 bp DNA SYN 23-Aug-2007 DEFINITION pTriEx-3 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5082 /organism="pTriEx-3" /mol_type="other DNA" misc_feature 47..1001 /label="Ac_5_flank" CDS complement(343..948) /label="ORF frame 1" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1080..1367 /label="CAG_enhancer" promoter 1025..1577 /label="CMV_immearly_promoter" misc_feature 1534..1554 /label="CMV_fwd_primer" promoter 1523..1604 /label="CMV_promoter" misc_feature 1870..1889 /label="pCAG_F_primer" promoter 1931..1949 /label="T7_promoter" misc_feature 1949..1976 /label="lacO" terminator 2590..2625 /label="T7_terminator" misc_feature complement(2648..2665) /label="Baculovirs_rev_primer" misc_feature 2655..2732 /label="3_AcPH" CDS complement(2762..3379) /label="ORF frame 2" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" misc_feature 3359..3392 /label="loxP" rep_origin complement(3545..4155) /label="pBR322_origin" CDS complement(4160..5020) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(4160..5020) /label="Ampicillin" /gene="Ampicillin" ORIGIN 1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga 61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat 121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca 181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa 241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt 301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa 361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag 421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt 481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt 541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt 601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg 661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc 721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt 781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt 841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc 901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg 961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggcct 1021 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta 1081 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt 1141 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg 1201 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc 1261 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga 1321 ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgc 1381 tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc 1441 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact 1501 ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt 1561 gggaggtcta tataagcaga cgtcgtttag tgaaccgtca gatcactaga tgctttattg 1621 cggtagttta tcacagttaa attgctaacg ccagtctcga acttaacgtg cagaagttgg 1681 tcgtgaggca ctgggcaggt aagtatcggg ccctttgtgc ggggggagcg gctcggggct 1741 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc 1801 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac 1861 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattggatc 1921 ggaccgaaat taatacgact cactataggg gaattgtgag cggataacaa ttccccggag 1981 ttaatccggg acctttaatt caacccaaca caatatatta tagttaaata agaattatta 2041 tcaaatcatt tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatca 2101 aaggagatat accatggcga tatcccggga gctcgtggat ccgaattctc agatctcggc 2161 gcgcctgcag gtcgacggta ccggttcgaa gcttgcggcc gcacagctgt atacacgtgc 2221 aagccagcca gaactcgccc cggaagaccc cgaggatctc gagcaccacc atcaccatca 2281 ccatcactaa gtgattaacc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg 2341 gccaatgccc tggctcacaa ataccactga gatcgatctt tttccctctg ccaaaaatta 2401 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt 2461 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc 2521 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat 2581 gtaactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagc 2641 atgcggagga aattctcctt gaagtttccc tggtgttcaa agtaaaggag tttgcaccag 2701 acgcacctct gttcactggt ccggcgtatt aaaacacgat acattgttat tagtacattt 2761 attaagcgct agattctgtg cgttgttgat ttacagacaa ttgttgtacg tattttaata 2821 attcattaaa tttataatct ttagggtggt atgttagagc gaaaatcaaa tgattttcag 2881 cgtctttata tctgaattta aatattaaat cctcaataga tttgtaaaat aggtttcgat 2941 tagtttcaaa caagggttgt ttttccgaac cgatggctgg actatctaat ggattttcgc 3001 tcaacgccac aaaacttgcc aaatcttgta gcagcaatct agctttgtcg atattcgttt 3061 gtgttttgtt ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct tttgtatttc 3121 tttcatcact gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat agagcttgga 3181 catatttaac atcgggcgtg ttagctttat taggccgatt atcgtcgtcg tcccaaccct 3241 cgtcgttaga agttgcttcc gaagacgatt ttgccatagc cacacgacgc ctattaattg 3301 tgtcggctaa cacgtccgcg atcaaatttg tagttgagct ttttggaatt gcgatcgcat 3361 aacttcgtat agcatacatt atacgaagtt ataagctcgg aacgctgcgc tcggtcgttc 3421 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 3481 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 3541 aggccgcgtt gctggcgttt ttccataggc tccgccccct gacgagcatc acaaaaatcg 3601 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 3661 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 3721 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc 3781 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 3841 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 3901 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 3961 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 4021 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 4081 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 4141 atctcaagaa gatcctttgt taccaatgct taatcagtga ggcacctatc tcagcgatct 4201 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 4261 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 4321 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 4381 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 4441 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 4501 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 4561 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 4621 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 4681 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 4741 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 4801 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 4861 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 4921 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 4981 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 5041 attgaagcat ttatcagggt tattgtctca tgtccgcgcg tt // pTriEx-3 "Times New Roman*]C/ Ac_5_flank"Times New Roman*]CWORF1Times New Roman*])CMV_immearly_promoter"Times New Roman*]CɆ8W CAG_enhancer"Times New Roman*]CD CMV_promoter"Times New Roman*]CCMV_fwd_primer"Times New Roman)*]CɆNa pCAG_F_primer"Times New Roman*] T7_promoter"Times New Roman*]ClacO"Times New Roman*]C A  T7_terminator"Times New Roman*]CɆX i Baculovirs_rev_primer"Times New Roman*]_ 3_AcPH"Times New Roman*]C 3  Orf frame 2"Times New Roman*]C @ loxP"Times New Roman*]CɆ ; pBR322_origin"Times New Roman*]@ORF2Times New Roman*]C@ Ampicillin"Times New Roman *]ApaI(1712), SacII(1746)"Times New Roman<^MiXcXTr *]XbaI"Times New Roman2mByQQ *]NcoI(2112), EcoRV(2121), SmaI(2126), SacI(2133), BamHI(2137), EcoRI(2143), BglII(2151), PstI(2169), AgeI(2180), KpnI(2181), BstBI(2186), HindIII(2189), NotI(2196), EagI(2196), PvuII(2206), PmlI(2216), XhoI(2258)"Times New RomanQh *]MscI(2341), ClaI(2373)"Times New RomanE [   *]test"Times New RomanO }ss6 *]test"Times New Roman~ yoc 6 *]test"Times New RomanYF)\)