Sequence of pTriEx-3

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pTriEx-3

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pTriEx-3 : 5082 BP; 1334 A; 1144 C; 1130 G; 1474 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga 60

1 aggacgtaga aaattagttt agggttctac acatatttgc gcggccatac atgtccttct 60

.......... .......... .......... .......... .......... Ac_5_flank

.......... .......... .......... .......... .......... ..........

61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat 120

61 ccaaatatga tttgacaatg taacgtttgc accaaagcac acggttcaca cttttggcta 120

.......... .......... Ac_5_flank .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca 180

121 caaattagtt ccgagactgc gtaaagatgt tggtgctgag gttcacacac ccacttcagt 180

.......... .......... Ac_5_flank .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa 240

181 acgtagaaaa ttagtttagg gttctacaca tatttggtgg tttgacggtt ttttactttt 240

.......... .......... Ac_5_flank .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt 300

241 gacagctgtt cgagacaggc aaacgaccgt tgacgttccc agagttagga taaacattaa 300

.......... .......... Ac_5_flank .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa 360

301 taacttatta ttttgttaat atttacagtt taaacaaaaa ataattgcta tgtttggttt 360

.......... .......... Ac_5_flank .......... .......... ORF1

.......... .......... .......... .......... .......... ..........

361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag 420

361 gcgttgttct tgtaaacatc ataatagata ttaacttttg cgcatcaata ttagcgactc 420

.......... ....... Ac_5_flank ORF1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt 480

421 cattataaat tttagtaaaa gtttactaag tgtcaattaa acgctgttat attaaaataa 480

.......... ....... Ac_5_flank ORF1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt 540

481 aagtgtattt gatctgcgga acagcagaag aagaagcata aggaagagaa aaagtaaaaa 540

.......... ....... Ac_5_flank ORF1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt 600

541 gagaagtatt tttaattgta tcaataatag cataggtata tacatagata gcatatctca 600

.......... ....... Ac_5_flank ORF1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg 660

601 tttaaaaaac aacagtattt atatatacag aaaaaattac cccacatatc atggcgacgc 660

.......... ....... Ac_5_flank ORF1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc 720

661 gtatcaaaaa gacattaaat gttgtcacga taaaagacca tcaagaagcc tcacacaacg 720

.......... ....... Ac_5_flank ORF1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt 780

721 aaattaataa tttaaatata ttagttactt aaaccctagc agccaaaaca tgttatacaa 780

.......... ....... Ac_5_flank ORF1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt 840

781 cggccgtatc atgcgtcgaa gaagatcaag ttaatgtggt aaaaaatcgt cgtggcctaa 840

.......... ....... Ac_5_flank ORF1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc 900

841 ttgtattgaa aggttttaca acatgcttgg caatttgttt ttgtcaagtg gagggaaaag 900

.......... ....... Ac_5_flank ORF1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg 960

901 atatgataac agacgctcgt caacaaacaa caatttttat tgtcggtaac attactctgc 960

.......... ....... Ac_5_flank ORF1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggcct 1020

961 gtgtttgatt atagtgtttg acctttacag atagttatat atcaacgact accggccgga 1020

.......... .......... Ac_5_flank .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta 1080

1021 taattatcat tagttaatgc cccagtaatc aagtatcggg tatatacctc aaggcgcaat 1080

.......... .......... .... CMV_immearly_promoter .... ..........

.......... .......... .......... .......... .......... ..........

1081 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt 1140

1081 gtattgaatg ccatttaccg ggcggaccga ctggcgggtt gctgggggcg ggtaactgca 1140

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

1141 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg 1200

1141 gttattactg catacaaggg tatcattgcg gttatccctg aaaggtaact gcagttaccc 1200

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

1201 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc 1260

1201 acctcataaa tgccatttga cgggtgaacc gtcatgtagt tcacatagta tacggttcag 1260

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

1261 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga 1320

1261 gcgggggata actgcagtta ctgccattta ccgggcggac cgtaatacgg gtcatgtact 1320

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

1321 ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgc 1380

1321 ggaatgccct gaaaggatga accgtcatgt agatgcataa tcagtagcga taatggtacg 1380

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

1381 tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc 1440

1381 actacgccaa aaccgtcatg tggttacccg cacctatcgc caaactgagt gcccctaaag 1440

.......... .... CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact 1500

1441 gttcagaggt ggggtaactg cagttaccct caaacaaaac cgtggtttta gttgccctga 1500

.......... .... CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt 1560

1501 aaggttttac agcattattg gggcggggca actgcgttta cccgccatcc gcacatgcca 1560

......... CMV_immearly_promoter CMV_promoter CMV_fwd_primer .

.......... .......... .......... .......... .......... ..........

1561 gggaggtcta tataagcaga cgtcgtttag tgaaccgtca gatcactaga tgctttattg 1620

1561 ccctccagat atattcgtct gcagcaaatc acttggcagt ctagtgatct acgaaataac 1620

CMV_immearly_promoter CMV_promoter ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cggtagttta tcacagttaa attgctaacg ccagtctcga acttaacgtg cagaagttgg 1680

1621 gccatcaaat agtgtcaatt taacgattgc ggtcagagct tgaattgcac gtcttcaacc 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tcgtgaggca ctgggcaggt aagtatcggg ccctttgtgc ggggggagcg gctcggggct 1740

1681 agcactccgt gacccgtcca ttcatagccc gggaaacacg cccccctcgc cgagccccga 1740

.......... .......... .......... .......... .......... ..........

SacII(1746) ........ .......... .......... .......... ..........

1741 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc 1800

1741 caggcgcccc cctgccgacg gaagcccccc ctgccccgtc ccgccccaag ccgaagaccg 1800

.......... .......... .......... .......... .......... ..........

.......... XbaI(1815) .......... .......... .......... ..........

1801 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac 1860

1801 cacactggcc gccgagatct cggagacgat tggtacaagt acggaagaag aaaaaggatg 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattggatc 1920

1861 tcgaggaccc gttgcacgac caataacacg acagagtagt aaaaccgttt cttaacctag 1920

........ pCAG_F_primer ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggaccgaaat taatacgact cactataggg gaattgtgag cggataacaa ttccccggag 1980

1921 cctggcttta attatgctga gtgatatccc cttaacactc gcctattgtt aaggggcctc 1980

......... T7_promoter ......... .......... lacO ..........

.......... .......... .......... .......... .......... ..........

1981 ttaatccggg acctttaatt caacccaaca caatatatta tagttaaata agaattatta 2040

1981 aattaggccc tggaaattaa gttgggttgt gttatataat atcaatttat tcttaataat 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcaaatcatt tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatca 2100

2041 agtttagtaa acatataatt aattttatga tatgacattt aatgtaaaat aaatgttagt 2100

.......... .......... .......... .......... .......... ..........

NcoI EcoRV,SmaI SacI2133),BamHI(2137) EcoRI(2143)

2101 aaggagatat accatggcga tatcccggga gctcgtggat ccgaattctc agatctcggc 2160

2101 ttcctctata tggtaccgct atagggccct cgagcaccta ggcttaagag tctagagccg 2160

.......... .......... .......... .......... .......... ..........

PstI AgeI,KpnI,BstBI,HindIII NotI,EagI PvuII2206)

2161 gcgcctgcag gtcgacggta ccggttcgaa gcttgcggcc gcacagctgt atacacgtgc 2220

2161 cgcggacgtc cagctgccat ggccaagctt cgaacgccgg cgtgtcgaca tatgtgcacg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... XhoI(2258) .......... ..........

2221 aagccagcca gaactcgccc cggaagaccc cgaggatctc gagcaccacc atcaccatca 2280

2221 ttcggtcggt cttgagcggg gccttctggg gctcctagag ctcgtggtgg tagtggtagt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccatcactaa gtgattaacc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg 2340

2281 ggtagtgatt cactaattgg agtccacgtc cgacggatag tcttccacca ccgaccacac 2340

.......... .......... .......... .......... .......... ..........

MscI(2341) .......... .......... ClaI(2373) .......... ..........

2341 gccaatgccc tggctcacaa ataccactga gatcgatctt tttccctctg ccaaaaatta 2400

2341 cggttacggg accgagtgtt tatggtgact ctagctagaa aaagggagac ggtttttaat 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt 2460

2401 acccctgtag tacttcgggg aactcgtaga ctgaagaccg attatttcct ttaaataaaa 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc 2520

2461 gtaacgttat cacacaacct taaaaaacac agagagtgag ccttcctgta taccctcccg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat 2580

2521 tttagtaaat tttgtagtct tactcataaa ccaaatctca aaccgttgta tacgggtata 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gtaactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagc 2640

2581 cattgatcgt attggggaac cccggagatt tgcccagaac tccccaaaaa acgactttcg 2640

.......... ........ T7_terminator ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 atgcggagga aattctcctt gaagtttccc tggtgttcaa agtaaaggag tttgcaccag 2700

2641 tacgcctcct ttaagaggaa cttcaaaggg accacaagtt tcatttcctc aaacgtggtc 2700

.... Baculovirs_rev_primer .... 3_AcPH .......... ..........

.......... .......... .......... .......... .......... ..........

2701 acgcacctct gttcactggt ccggcgtatt aaaacacgat acattgttat tagtacattt 2760

2701 tgcgtggaga caagtgacca ggccgcataa ttttgtgcta tgtaacaata atcatgtaaa 2760

.......... 3_AcPH .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 attaagcgct agattctgtg cgttgttgat ttacagacaa ttgttgtacg tattttaata 2820

2761 taattcgcga tctaagacac gcaacaacta aatgtctgtt aacaacatgc ataaaattat 2820

.......... .......... ......... Orf frame 2 ......... ..........

.......... .......... .......... .......... .......... ..........

2821 attcattaaa tttataatct ttagggtggt atgttagagc gaaaatcaaa tgattttcag 2880

2821 taagtaattt aaatattaga aatcccacca tacaatctcg cttttagttt actaaaagtc 2880

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgtctttata tctgaattta aatattaaat cctcaataga tttgtaaaat aggtttcgat 2940

2881 gcagaaatat agacttaaat ttataattta ggagttatct aaacatttta tccaaagcta 2940

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tagtttcaaa caagggttgt ttttccgaac cgatggctgg actatctaat ggattttcgc 3000

2941 atcaaagttt gttcccaaca aaaaggcttg gctaccgacc tgatagatta cctaaaagcg 3000

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tcaacgccac aaaacttgcc aaatcttgta gcagcaatct agctttgtcg atattcgttt 3060

3001 agttgcggtg ttttgaacgg tttagaacat cgtcgttaga tcgaaacagc tataagcaaa 3060

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gtgttttgtt ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct tttgtatttc 3120

3061 cacaaaacaa aacattattt ccaagctgca gcaagtttta taatacgcga aaacataaag 3120

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tttcatcact gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat agagcttgga 3180

3121 aaagtagtga cagcaatcac atgttaactg agctgcattt gtgcaattta tctcgaacct 3180

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 catatttaac atcgggcgtg ttagctttat taggccgatt atcgtcgtcg tcccaaccct 3240

3181 gtataaattg tagcccgcac aatcgaaata atccggctaa tagcagcagc agggttggga 3240

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cgtcgttaga agttgcttcc gaagacgatt ttgccatagc cacacgacgc ctattaattg 3300

3241 gcagcaatct tcaacgaagg cttctgctaa aacggtatcg gtgtgctgcg gataattaac 3300

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tgtcggctaa cacgtccgcg atcaaatttg tagttgagct ttttggaatt gcgatcgcat 3360

3301 acagccgatt gtgcaggcgc tagtttaaac atcaactcga aaaaccttaa cgctagcgta 3360

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aacttcgtat agcatacatt atacgaagtt ataagctcgg aacgctgcgc tcggtcgttc 3420

3361 ttgaagcata tcgtatgtaa tatgcttcaa tattcgagcc ttgcgacgcg agccagcaag 3420

Orf frame 2 loxP ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 3480

3421 ccgacgccgc tcgccatagt cgagtgagtt tccgccatta tgccaatagg tgtcttagtc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 3540

3481 ccctattgcg tcctttcttg tacactcgtt ttccggtcgt tttccggtcc ttggcatttt 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aggccgcgtt gctggcgttt ttccataggc tccgccccct gacgagcatc acaaaaatcg 3600

3541 tccggcgcaa cgaccgcaaa aaggtatccg aggcggggga ctgctcgtag tgtttttagc 3600

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3601 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 3660

3601 tgcgagttca gtctccaccg ctttgggctg tcctgatatt tctatggtcc gcaaaggggg 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 3720

3661 accttcgagg gagcacgcga gaggacaagg ctgggacggc gaatggccta tggacaggcg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc 3780

3721 gaaagaggga agcccttcgc accgcgaaag agttacgagt gcgacatcca tagagtcaag 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 3840

3781 ccacatccag caagcgaggt tcgacccgac acacgtgctt ggggggcaag tcgggctggc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 3900

3841 gacgcggaat aggccattga tagcagaact caggttgggc cattctgtgc tgaatagcgg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 3960

3901 tgaccgtcgt cggtgaccat tgtcctaatc gtctcgctcc atacatccgc cacgatgtct 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 4020

3961 caagaacttc accaccggat tgatgccgat gtgatcttct tgtcataaac catagacgcg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 4080

4021 agacgacttc ggtcaatgga agcctttttc tcaaccatcg agaactaggc cgtttgtttg 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 4140

4081 gtggcgacca tcgccaccaa aaaaacaaac gttcgtcgtc taatgcgcgt ctttttttcc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 atctcaagaa gatcctttgt taccaatgct taatcagtga ggcacctatc tcagcgatct 4200

4141 tagagttctt ctaggaaaca atggttacga attagtcact ccgtggatag agtcgctaga 4200

pBR322_origin ...... ....... ORF2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4201 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 4260

4201 cagataaagc aagtaggtat caacggactg aggggcagca catctattga tgctatgccc 4260

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 4320

4261 tcccgaatgg tagaccgggg tcacgacgtt actatggcgc tctgggtgcg agtggccgag 4320

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 4380

4321 gtctaaatag tcgttatttg gtcggtcggc cttcccggct cgcgtcttca ccaggacgtt 4380

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 4440

4381 gaaataggcg gaggtaggtc agataattaa caacggccct tcgatctcat tcatcaagcg 4440

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 4500

4441 gtcaattatc aaacgcgttg caacaacggt aacgatgtcc gtagcaccac agtgcgagca 4500

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 4560

4501 gcaaaccata ccgaagtaag tcgaggccaa gggttgctag ttccgctcaa tgtactaggg 4560

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 4620

4561 ggtacaacac gttttttcgc caatcgagga agccaggagg ctagcaacag tcttcattca 4620

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 4680

4621 accggcgtca caatagtgag taccaatacc gtcgtgacgt attaagagaa tgacagtacg 4680

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 4740

4681 gtaggcattc tacgaaaaga cactgaccac tcatgagttg gttcagtaag actcttatca 4740

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 4800

4741 catacgccgc tggctcaacg agaacgggcc gcagttatgc cctattatgg cgcggtgtat 4800

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 4860

4801 cgtcttgaaa ttttcacgag tagtaacctt ttgcaagaag ccccgctttt gagagttcct 4860

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 4920

4861 agaatggcga caactctagg tcaagctaca ttgggtgagc acgtgggttg actagaagtc 4920

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 4980

4921 gtagaaaatg aaagtggtcg caaagaccca ctcgtttttg tccttccgtt ttacggcgtt 4980

.......... ....... ORF2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 5040

4981 ttttccctta ttcccgctgt gcctttacaa cttatgagta tgagaaggaa aaagttataa 5040

....... ORF2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 attgaagcat ttatcagggt tattgtctca tgtccgcgcg tt........ .......... 5082

5041 taacttcgta aatagtccca ataacagagt acaggcgcgc aa........ .......... 5082

.......... .......... .......... .......... .......... ..........