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    Plasmid map of pU6-mir30

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    Gene/insert name: human U6 promoter-mir30 cassette
    Insert size (bp): 666
    Vector backbone: pCAGEN
    Type of vector: Mammalian expression,RNAi
    Backbone size (bp): 2524
    Cloning site 5': SalI
    Site destroyed during cloning: No
    Cloning site 3': PstI
    Site destroyed during cloning: No
    5' Sequencing primer: N/A
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: The mir30 cassette with the human U6 promoter was amplified by PCR using pSM2 (Open Biosystems) as a template.
    Plasmid Provided In: DH5a
    Principal Investigator: Connie Cepko
    Comments: Haipin DNA can be cloned into the XhoI/EcoRI sites. A protocol for hairpin DNA design and cloning was reported by Gregory Hannon lab (Paddison et al. Nat. Methods 1, 163-167 (2004)).
    Article: Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T et al. (Proc Natl Acad Sci U S A. 2007 Jan 5. ():. Pubmed)

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