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pU6-mir30
Gene/insert name: human U6 promoter-mir30 cassette
Insert size (bp): 666
Vector backbone: pCAGEN
Type of vector: Mammalian expression,RNAi
Backbone size (bp): 2524
Cloning site 5': SalI
Site destroyed during cloning: No
Cloning site 3': PstI
Site destroyed during cloning: No
5' Sequencing primer: N/A
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: The mir30 cassette with the human U6 promoter was amplified by PCR using pSM2 (Open Biosystems) as a template.
Plasmid Provided In: DH5a
Principal Investigator: Connie Cepko
Comments: Haipin DNA can be cloned into the XhoI/EcoRI sites. A protocol for hairpin DNA design and cloning was reported by Gregory Hannon lab (Paddison et al. Nat. Methods 1, 163-167 (2004)).
Article: Controlled expression of transgenes introduced by in vivo electroporation. Matsuda T et al. (Proc Natl Acad Sci U S A. 2007 Jan 5. ():. Pubmed)