MpMpLOCUS pU6-mir30 Sequence 3190 bp DNA SYN 25-Aug-2007 DEFINITION pU6-mir30 Sequence ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..3190 /organism="pU6-mir30 Sequence" /mol_type="other DNA" promoter 104..361 /label="hU6_promoter" misc_feature 291..310 /label="LKO1_5_primer" misc_feature complement(689..707) /label="M13_reverse_primer" misc_feature complement(706..728) /label="M13_pUC_rev_primer" promoter complement(742..771) /label="lac_promoter" rep_origin 912..989 /label="SV40_origin" promoter 877..1056 /label="SV40_promoter" misc_feature 974..993 /label="SV40pro_F_primer" terminator 1070..1201 /label="SV40_PA_terminator" misc_feature 1158..1177 /label="EBV_rev_primer" rep_origin complement(1425..2044) /label="pBR322_origin" CDS complement(2199..3059) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(2199..3059) /label="Ampicillin" /gene="Ampicillin" promoter complement(3101..3129) /label="AmpR_promoter" ORIGIN 1 GTCGACATTG ATTATTGAct agcatcgatg atatcCCCAG TGGAAAGACG CGCAGGCAAA 61 ACGCACCACG TGACGGAGCG TGACCGCGCG CCGAGCGCGC GCCAAGGTCG GGCAGGAAGA 121 GGGCCTATTT CCCATGATTC CTTCATATTT GCATATACGA TACAAGGCTG TTAGAGAGAT 181 AATTAGAATT AATTTGACTG TAAACACAAA GATATTAGTA CAAAATACGT GACGTAGAAA 241 GTAATAATTT CTTGGGTAGT TTGCAGTTTT TAAAATTATG TTTTAAAATG GACTATCATA 301 TGCTTACCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC 361 GAAACACCGT GCTCGCTTCG GCAGCACATA TACTAGTCGA CTAGGGATAA CAGGGTAATT 421 GTTTGAATGA GGCTTCAGTA CTTTACAGAA TCGTTGCCTG CACATCTTGG AAACACTTGC 481 TGGGATTACT TCTTCAGGTT AACCCAACAG AAGGCTCGAG CAACCAGAAT TCAAGGGGCT 541 ACTTTAGGAG CAATTATCTT GTTTACTAAA ACTGAATACC TTGCTATCTC TTTGATACAT 601 TTTTACAAAG CTGAATTAAA ATGGTATAAA TTAAATCACT TTTTTgatat catcgatgcg 661 gccgcctgca gCCCAAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT 721 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT 781 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG 841 GGAAACCTGT CGTGCCAGCG GATCCGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC 901 TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT 961 GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA 1021 AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTAACT TGTTTATTGC 1081 AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT 1141 TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT 1201 CCGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT 1261 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA 1321 GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC 1381 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT 1441 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG 1501 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC 1561 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC 1621 GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC 1681 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC 1741 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT 1801 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT 1861 AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC 1921 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT 1981 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG 2041 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC 2101 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA 2161 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG 2221 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG 2281 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA 2341 GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG 2401 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA 2461 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC 2521 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA 2581 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG 2641 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT 2701 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC 2761 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG 2821 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG 2881 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT 2941 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA 3001 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA 3061 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 3121 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 3181 GTGCCACCTG //v pU6-mir30 "Times New RomanMpCv hi hU6_promoter"Times New RomanMpCv #6 LKO1_5_primer"Times New RomanMpCɆv M13_rev"Times New RomanMpv  M13_pUC_rev"Times New RomanMpCv  lac_promoter"Times New RomanMpCɆv m  SV40_promoter"Times New RomanMpCv  SV40_origin"Times New RomanMpv  SV40pro_F"Times New Roman)MpCv .SV40_PA_terminator"Times New RomanMpCv EBV_rev_primer"Times New RomanMpCɆv  pBR322_origin"Times New RomanMpv   Orf frame 3"Times New Roman(MpCv   Ampicillin"Times New RomanMpCv  9  AmpR_promoter"Times New Roman MpPmlI"Times New RomanEv N:&& MpNdeI"Times New Roman*+v raPATP MpSpeI"Times New Roman2v  (6CqCb{q Mp HpaI(500), XhoI(515), EcoRI(527)"Times New RomanBv L]mmw Mp-NotI(659), EagI(659), PstI(670), HindIII(675)"Times New RomanNv fz MpNcoI"Times New Romanvv ezx} MpStuI"Times New Romanv Vhyy MpFspI"Times New Roman $v  z<z Mptest"Times New RomanOv Z3hm Mptest"Times New Roman v _l _RR';