Sequence of pU6-mir30

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Sequence of pU6-mir30

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Sequence of pU6-mir30 : 3190 BP; 832 A; 774 C; 734 G; 850 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gtcgacattg attattgact agcatcgatg atatccccag tggaaagacg cgcaggcaaa 60

1 cagctgtaac taataactga tcgtagctac tataggggtc acctttctgc gcgtccgttt 60

.......... .......... .......... .......... .......... ..........

PmlI(69) .......... .......... .......... .......... ..........

61 acgcaccacg tgacggagcg tgaccgcgcg ccgagcgcgc gccaaggtcg ggcaggaaga 120

61 tgcgtggtgc actgcctcgc actggcgcgc ggctcgcgcg cggttccagc ccgtccttct 120

.......... .......... .......... .......... ......... hU6_promoter

.......... .......... .......... .......... .......... ..........

121 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat 180

121 cccggataaa gggtactaag gaagtataaa cgtatatgct atgttccgac aatctctcta 180

.......... ......... hU6_promoter ......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa 240

181 ttaatcttaa ttaaactgac atttgtgttt ctataatcat gttttatgca ctgcatcttt 240

.......... ......... hU6_promoter ......... .......... ..........

.......... .......... .......... .......... .......... NdeI(298)

241 gtaataattt cttgggtagt ttgcagtttt taaaattatg ttttaaaatg gactatcata 300

241 cattattaaa gaacccatca aacgtcaaaa attttaatac aaaattttac ctgatagtat 300

.......... ......... hU6_promoter ......... ........ LKO1_5_primer

.......... .......... .......... .......... .......... ..........

301 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac 360

301 acgaatggca ttgaactttc ataaagctaa agaaccgaaa tatatagaac acctttcctg 360

LKO1_5_primer ..... hU6_promoter ......... .......... ..........

.......... .......... .......... SpeI(392) .......... ..........

361 gaaacaccgt gctcgcttcg gcagcacata tactagtcga ctagggataa cagggtaatt 420

361 ctttgtggca cgagcgaagc cgtcgtgtat atgatcagct gatccctatt gtcccattaa 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gtttgaatga ggcttcagta ctttacagaa tcgttgcctg cacatcttgg aaacacttgc 480

421 caaacttact ccgaagtcat gaaatgtctt agcaacggac gtgtagaacc tttgtgaacg 480

.......... .......... .......... .......... .......... ..........

.......... HpaI(500) .......... XhoI(515) EcoRI(527) ..........

481 tgggattact tcttcaggtt aacccaacag aaggctcgag caaccagaat tcaaggggct 540

481 accctaatga agaagtccaa ttgggttgtc ttccgagctc gttggtctta agttccccga 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 actttaggag caattatctt gtttactaaa actgaatacc ttgctatctc tttgatacat 600

541 tgaaatcctc gttaatagaa caaatgattt tgacttatgg aacgatagag aaactatgta 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ttttacaaag ctgaattaaa atggtataaa ttaaatcact tttttgatat catcgatgcg 660

601 aaaatgtttc gacttaattt taccatattt aatttagtga aaaaactata gtagctacgc 660

.......... .......... .......... .......... .......... ..........

.... PstI(670),HindIII(675) .... .......... .......... ..........

661 gccgcctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 720

661 cggcggacgt cgggttcgaa ccgcattagt accagtatcg acaaaggaca cactttaaca 720

.......... .......... .......... M13_rev ......... M13_pUC_rev

.......... .......... .......... .......... .......... ..........

721 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 780

721 ataggcgagt gttaaggtgt gttgtatgct cggccttcgt atttcacatt tcggacccca 780

.......... .......... ......... lac_promoter ......... ..........

.......... .......... .......... .......... .......... ..........

781 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 840

781 cggattactc actcgattga gtgtaattaa cgcaacgcga gtgacgggcg aaaggtcagc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc 900

841 cctttggaca gcacggtcgc ctaggcgtag agttaatcag tcgttggtat cagggcgggg 900

.......... .......... .......... ........ SV40_promoter ........

.......... .......... .......... .......... .......... NcoI(953)

901 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 960

901 attgaggcgg gtagggcggg gattgaggcg ggtcaaggcg ggtaagaggc ggggtaccga 960

.......... ....... SV40_promoter SV40_origin ....... ..........

.......... .......... .......... .......... .......... ..........

961 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 1020

961 ctgattaaaa aaaataaata cgtctccggc tccggcggag ccggagactc gataaggtct 1020

.. SV40_origin SV40_promoter SV40pro_F .. .......... ..........

.......... .......... StuI(1045) .......... .......... ..........

1021 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc 1080

1021 tcatcactcc tccgaaaaaa cctccggatc cgaaaacgtt tttcgattga acaaataacg 1080

........ SV40_promoter ........ .......... ...... SV40_PA_terminator

.......... .......... .......... .......... .......... ..........

1081 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 1140

1081 tcgaatatta ccaatgttta tttcgttatc gtagtgttta aagtgtttat ttcgtaaaaa 1140

.......... ...... SV40_PA_terminator ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat 1200

1141 aagtgacgta agatcaacac caaacaggtt tgagtagtta catagaatag tacagaccta 1200

........ SV40_PA_terminator EBV_rev_primer ........ ..........

.......... .......... .......... .......... .......... ..........

1201 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 1260

1201 ggcgacgtaa ttacttagcc ggttgcgcgc ccctctccgc caaacgcata acccgcgaga 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 1320

1261 aggcgaagga gcgagtgact gagcgacgcg agccagcaag ccgacgccgc tcgccatagt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 1380

1321 cgagtgagtt tccgccatta tgccaatagg tgtcttagtc ccctattgcg tcctttcttg 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 1440

1381 tacactcgtt ttccggtcgt tttccggtcc ttggcatttt tccggcgcaa cgaccgcaaa 1440

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1441 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 1500

1441 aaggtatccg aggcgggggg actgctcgta gtgtttttag ctgcgagttc agtctccacc 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 1560

1501 gctttgggct gtcctgatat ttctatggtc cgcaaagggg gaccttcgag ggagcacgcg 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 1620

1561 agaggacaag gctgggacgg cgaatggcct atggacaggc ggaaagaggg aagcccttcg 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 1680

1621 caccgcgaaa gagttacgag tgcgacatcc atagagtcaa gccacatcca gcaagcgagg 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 1740

1681 ttcgacccga cacacgtgct tggggggcaa gtcgggctgg cgacgcggaa taggccattg 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 1800

1741 atagcagaac tcaggttggg ccattctgtg ctgaatagcg gtgaccgtcg tcggtgacca 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 1860

1801 ttgtcctaat cgtctcgctc catacatccg ccacgatgtc tcaagaactt caccaccgga 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 1920

1861 ttgatgccga tgtgatcttc ctgtcataaa ccatagacgc gagacgactt cggtcaatgg 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 1980

1921 aagccttttt ctcaaccatc gagaactagg ccgtttgttt ggtggcgacc atcgccacca 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 2040

1981 aaaaaacaaa cgttcgtcgt ctaatgcgcg tctttttttc ctagagttct tctaggaaac 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 2100

2041 tagaaaagat gccccagact gcgagtcacc ttgcttttga gtgcaattcc ctaaaaccag 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 2160

2101 tactctaata gtttttccta gaagtggatc taggaaaatt taatttttac ttcaaaattt 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 2220

2161 agttagattt catatatact catttgaacc agactgtcaa tggttacgaa ttagtcactc 2220

.......... .......... .......... .... Orf frame 3 Ampicillin ....

.......... .......... .......... .......... .......... ..........

2221 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 2280

2221 cgtggataga gtcgctagac agataaagca agtaggtatc aacggactga ggggcagcac 2280

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 2340

2281 atctattgat gctatgccct cccgaatggt agaccggggt cacgacgtta ctatggcgct 2340

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 2400

2341 ctgggtgcga gtggccgagg tctaaatagt cgttatttgg tcggtcggcc ttcccggctc 2400

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 2460

2401 gcgtcttcac caggacgttg aaataggcgg aggtaggtca gataattaac aacggccctt 2460

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... FspI(2494) .......... ..........

2461 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 2520

2461 cgatctcatt catcaagcgg tcaattatca aacgcgttgc aacaacggta acgatgtccg 2520

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 2580

2521 tagcaccaca gtgcgagcag caaaccatac cgaagtaagt cgaggccaag ggttgctagt 2580

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 2640

2581 tccgctcaat gtactagggg gtacaacacg ttttttcgcc aatcgaggaa gccaggaggc 2640

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 2700

2641 tagcaacagt cttcattcaa ccggcgtcac aatagtgagt accaataccg tcgtgacgta 2700

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 2760

2701 ttaagagaat gacagtacgg taggcattct acgaaaagac actgaccact catgagttgg 2760

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 2820

2761 ttcagtaaga ctcttatcac atacgccgct ggctcaacga gaacgggccg cagttatgcc 2820

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 2880

2821 ctattatggc gcggtgtatc gtcttgaaat tttcacgagt agtaaccttt tgcaagaagc 2880

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 2940

2881 cccgcttttg agagttccta gaatggcgac aactctaggt caagctacat tgggtgagca 2940

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 3000

2941 cgtgggttga ctagaagtcg tagaaaatga aagtggtcgc aaagacccac tcgtttttgt 3000

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 3060

3001 ccttccgttt tacggcgttt tttcccttat tcccgctgtg cctttacaac ttatgagtat 3060

.......... .... Orf frame 3 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 3120

3061 gagaaggaaa aagttataat aacttcgtaa atagtcccaa taacagagta ctcgcctatg 3120

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

3121 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 3180

3121 tataaactta cataaatctt tttatttgtt tatccccaag gcgcgtgtaa aggggctttt 3180

AmpR_promoter ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gtgccacctg .......... .......... .......... .......... .......... 3190

3181 cacggtggac .......... .......... .......... .......... .......... 3190

.......... .......... .......... .......... .......... ..........