Sequence of pVR37

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Sequence of pVR37

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Sequence of pVR37: 12588 BP; 2957 A; 3389 C; 3384 G; 2858 T; 0 other;

BamHI(1) XbaI(7) SalI(13) PstI(23) .... .......... ..........

1 gatcctctag agtcgacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt 60

1 ctaggagatc tcagctggac gtccgtacgt tcgaaccgtg accggcagca aaatgttgca 60

.......... .......... .......... . M13_forward20_primer M13_pUC_fwd_primer

.......... .......... .......... .......... .......... ..........

61 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 120

61 gcactgaccc ttttgggacc gcaatgggtt gaattagcgg aacgtcgtgt agggggaaag 120

M13_pUC_fwd_primer .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 180

121 cggtcgaccg cattatcgct tctccgggcg tggctagcgg gaagggttgt caacgcgtcg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag 240

181 gacttaccgc ttacgatctc gtcgaactcg aacctagtct aacagcaaag ggcggaagtc 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tttagcttca tggagtcaaa gattcaaata gaggacctaa cagaactcgc cgtaaagact 300

241 aaatcgaagt acctcagttt ctaagtttat ctcctggatt gtcttgagcg gcatttctga 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ggcgaacagt tcatacagag tctcttacga ctcaatgaca agaagaaaat cttcgtcaac 360

301 ccgcttgtca agtatgtctc agagaatgct gagttactgt tcttctttta gaagcagttg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac 420

361 taccacctcg tgctgtgtga acagatgagg tttttatagt ttctatgtca gagtcttctg 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat 480

421 gtttcccgtt aactctgaaa agttgtttcc cattataggc ctttggagga gcctaaggta 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa 540

481 acgggtcgat agacagtgaa ataacacttc tatcaccttt tccttccacc gaggatgttt 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc 600

541 acggtagtaa cgctatttcc tttccggtag caacttctac ggagacggct gtcaccaggg 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 660

601 tttctacctg ggggtgggtg ctcctcgtag cacctttttc ttctgcaagg ttggtgcaga 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 720

661 agtttcgttc acctaactac actatagagg tgactgcatt ccctactgcg tgttagggtg 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gagaacacgg 780

721 ataggaagcg ttctgggaag gagatatatt ccttcaagta aagtaaacct ctcttgtgcc 780

.......... .......... .......... .......... .......... ..........

..... NcoI(793) BglII(800) ..... .......... .......... ..........

781 gggactcttg accatggtag atctgagggt aaatttctag tttttctcct tcattttctt 840

781 ccctgagaac tggtaccatc tagactccca tttaaagatc aaaaagagga agtaaaagaa 840

.......... ......... GUSPlus-His6 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggttaggacc cttttctctt tttatttttt tgagctttga tctttcttta aactgatcta 900

841 ccaatcctgg gaaaagagaa aaataaaaaa actcgaaact agaaagaaat ttgactagat 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ttttttaatt gattggttat ggtgtaaata ttacatagct ttaactgata atctgattac 960

901 aaaaaattaa ctaaccaata ccacatttat aatgtatcga aattgactat tagactaatg 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tttatttcgt gtgtctatga tgatgatgat agttacagaa ccgacgaact agtctgtacc 1020

961 aaataaagca cacagatact actactacta tcaatgtctt ggctgcttga tcagacatgg 1020

.......... .......... .......... .......... ORF 3 ..........

.......... .......... .......... .......... .......... ..........

1021 cgatcaacac cgagacccgt ggcgtcttcg acctcaatgg cgtctggaac ttcaagctgg 1080

1021 gctagttgtg gctctgggca ccgcagaagc tggagttacc gcagaccttg aagttcgacc 1080

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 actacgggaa aggactggaa gagaagtggt acgaaagcaa gctgaccgac actattagta 1140

1081 tgatgccctt tcctgacctt ctcttcacca tgctttcgtt cgactggctg tgataatcat 1140

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tggccgtccc aagcagttac aatgacattg gcgtgaccaa ggaaatccgc aaccatatcg 1200

1141 accggcaggg ttcgtcaatg ttactgtaac cgcactggtt cctttaggcg ttggtatagc 1200

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gatatgtctg gtacgaacgt gagttcacgg tgccggccta tctgaaggat cagcgtatcg 1260

1201 ctatacagac catgcttgca ctcaagtgcc acggccggat agacttccta gtcgcatagc 1260

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgctccgctt cggctctgca actcacaaag caattgtcta tgtcaatggt gagctggtcg 1320

1261 acgaggcgaa gccgagacgt tgagtgtttc gttaacagat acagttacca ctcgaccagc 1320

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tggagcacaa gggcggattc ctgccattcg aagcggaaat caacaactcg ctgcgtgatg 1380

1321 acctcgtgtt cccgcctaag gacggtaagc ttcgccttta gttgttgagc gacgcactac 1380

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gcatgaatcg cgtcaccgtc gccgtggaca acatcctcga cgatagcacc ctcccggtgg 1440

1381 cgtacttagc gcagtggcag cggcacctgt tgtaggagct gctatcgtgg gagggccacc 1440

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ggctgtacag cgagcgccac gaagagggcc tcggaaaagt cattcgtaac aagccgaact 1500

1441 ccgacatgtc gctcgcggtg cttctcccgg agccttttca gtaagcattg ttcggcttga 1500

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tcgacttctt caactatgca ggcctgcacc gtccggtgaa aatctacacg accccgttta 1560

1501 agctgaagaa gttgatacgt ccggacgtgg caggccactt ttagatgtgc tggggcaaat 1560

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cgtacgtcga ggacatctcg gttgtgaccg acttcaatgg cccaaccggg actgtgacct 1620

1561 gcatgcagct cctgtagagc caacactggc tgaagttacc gggttggccc tgacactgga 1620

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 atacggtgga ctttcaaggc aaagccgaga ccgtgaaagt gtcggtcgtg gatgaggaag 1680

1621 tatgccacct gaaagttccg tttcggctct ggcactttca cagccagcac ctactccttc 1680

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcaaagtggt cgcaagcacc gagggcctga gcggtaacgt ggagattccg aatgtcatcc 1740

1681 cgtttcacca gcgttcgtgg ctcccggact cgccattgca cctctaaggc ttacagtagg 1740

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tctgggaacc actgaacacg tatctctacc agatcaaagt ggaactggtg aacgacggac 1800

1741 agacccttgg tgacttgtgc atagagatgg tctagtttca ccttgaccac ttgctgcctg 1800

.......... ......... ORF 3 ORF 4 ......... .......... ..........

ClaI(1808) .......... .......... .......... .......... ..........

1801 tgaccatcga tgtctatgaa gagccgttcg gcgtgcggac cgtggaagtc aacgacggca 1860

1801 actggtagct acagatactt ctcggcaagc cgcacgcctg gcaccttcag ttgctgccgt 1860

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agttcctcat caacaacaaa ccgttctact tcaagggctt tggcaaacat gaggacactc 1920

1861 tcaaggagta gttgttgttt ggcaagatga agttcccgaa accgtttgta ctcctgtgag 1920

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctatcaacgg ccgtggcttt aacgaagcga gcaatgtgat ggatttcaat atcctcaaat 1980

1921 gatagttgcc ggcaccgaaa ttgcttcgct cgttacacta cctaaagtta taggagttta 1980

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ggatcggcgc caacagcttc cggaccgcac actatccgta ctctgaagag ttgatgcgtc 2040

1981 cctagccgcg gttgtcgaag gcctggcgtg tgataggcat gagacttctc aactacgcag 2040

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ttgcggatcg cgagggtctg gtcgtgatcg acgagactcc ggcagttggc gtgcacctca 2100

2041 aacgcctagc gctcccagac cagcactagc tgctctgagg ccgtcaaccg cacgtggagt 2100

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 acttcatggc caccacggga ctcggcgaag gcagcgagcg cgtcagtacc tgggagaaga 2160

2101 tgaagtaccg gtggtgccct gagccgcttc cgtcgctcgc gcagtcatgg accctcttct 2160

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ttcggacgtt tgagcaccat caagacgttc tccgtgaact ggtgtctcgt gacaagaacc 2220

2161 aagcctgcaa actcgtggta gttctgcaag aggcacttga ccacagagca ctgttcttgg 2220

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 atccaagcgt cgtgatgtgg agcatcgcca acgaggcggc gactgaggaa gagggcgcgt 2280

2221 taggttcgca gcactacacc tcgtagcggt tgctccgccg ctgactcctt ctcccgcgca 2280

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 acgagtactt caagccgttg gtggagctga ccaaggaact cgacccacag aagcgtccgg 2340

2281 tgctcatgaa gttcggcaac cacctcgact ggttccttga gctgggtgtc ttcgcaggcc 2340

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tcacgatcgt gctgtttgtg atggctaccc cggagacgga caaagtcgcc gaactgattg 2400

2341 agtgctagca cgacaaacac taccgatggg gcctctgcct gtttcagcgg cttgactaac 2400

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acgtcatcgc gctcaatcgc tataacggat ggtacttcga tggcggtgat ctcgaagcgg 2460

2401 tgcagtagcg cgagttagcg atattgccta ccatgaagct accgccacta gagcttcgcc 2460

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ccaaagtcca tctccgccag gaatttcacg cgtggaacaa gcgttgccca ggaaagccga 2520

2461 ggtttcaggt agaggcggtc cttaaagtgc gcaccttgtt cgcaacgggt cctttcggct 2520

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tcatgatcac tgagtacggc gcagacaccg ttgcgggctt tcacgacatt gatccagtga 2580

2521 agtactagtg actcatgccg cgtctgtggc aacgcccgaa agtgctgtaa ctaggtcact 2580

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgttcaccga ggaatatcaa gtcgagtact accaggcgaa ccacgtcgtg ttcgatgagt 2640

2581 acaagtggct ccttatagtt cagctcatga tggtccgctt ggtgcagcac aagctactca 2640

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... NruI(2686) ..........

2641 ttgagaactt cgtgggtgag caagcgtgga acttcgcgga cttcgcgacc tctcagggcg 2700

2641 aactcttgaa gcacccactc gttcgcacct tgaagcgcct gaagcgctgg agagtcccgc 2700

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgatgcgcgt ccaaggaaac aagaagggcg tgttcactcg tgaccgcaag ccgaagctcg 2760

2701 actacgcgca ggttcctttg ttcttcccgc acaagtgagc actggcgttc ggcttcgagc 2760

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... NruI(2777) .......... .......... .......... ..........

2761 ccgcgcacgt ctttcgcgag cgctggacca acattccaga tttcggctac aagaacgcta 2820

2761 ggcgcgtgca gaaagcgctc gcgacctggt tgtaaggtct aaagccgatg ttcttgcgat 2820

.......... ......... ORF 3 ORF 4 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gccatcacca tcaccatcac gtgtgaattg gtgaccagct cgaatttccc cgatcgttca 2880

2821 cggtagtggt agtggtagtg cacacttaac cactggtcga gcttaaaggg gctagcaagt 2880

.......... ORF 4 ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc 2940

2881 ttgtaaaccg ttatttcaaa gaattctaac ttaggacaac ggccagaacg ctactaatag 2940

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta 3000

2941 tatattaaag acaacttaat gcaattcgta cattattaat tgtacattac gtactgcaat 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa 3060

3001 aaatactcta cccaaaaata ctaatctcag ggcgttaata tgtaaattat gcgctatctt 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta 3120

3061 ttgttttata tcgcgcgttt gatcctattt aatagcgcgc gccacagtag atacaatgat 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gatcgggaat taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa 3180

3121 ctagccctta atttgatagt cacaaactgt cctatataac cgcccatttg gattctcttt 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agagcgttta ttagaataac ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc 3240

3181 tctcgcaaat aatcttattg cctataaatt ttcccgcact tttccaaata ggcaagcagg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc 3300

3241 taaacataca cgtacggttg gtgtcccaag gggagcccta gtttcatgaa actaggttgg 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga 3360

3301 ggaggcgacg atatcacgtc agccgaagac tgcaagtcac gtcggcagaa gacttttgct 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt 3420

3361 gtacagcgtg ttcaggattc aatgcgctgt ccgacggcgg gacgggaaaa ggaccgcaaa 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta 3480

3421 agaacagcgc acaaaatcag cgtatttcat cttatgaacg ctgatcttgg cctctgtaat 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga 3540

3481 gcggtacttg ttctcgcggc ggcgaccgga cgacccgata cgggcgcagt cgtggctgct 3540

.......... .......... .......... ORF 2 .......... ..........

.......... .......... .......... EagI(3577) .......... ..........

3541 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc 3600

3541 ggtcctgaac tggttggttg cccggcttga cgtgcgccgg ccgacgtggt tcgacaaaag 3600

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct 3660

3601 gctcttctag tggccgtggt ccgcgctggc gggcctcgac cggtcctacg aactggtgga 3660

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga 3720

3661 tgcgggaccg ctgcaacact gtcactggtc cgatctggcg gaccgggcgt cgtgggcgct 3720

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga 3780

3721 ggatgacctg taacggctcg cgtaggtcct ccggccgcgc ccggacgcat cggaccgtct 3780

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat 3840

3781 cggcacccgg ctgtggtggt gcggccggcc ggcgtaccac aactggcaca agcggccgta 3840

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa 3900

3841 acggctcaag ctcgcaaggg attagtagct ggcgtgggcc tcgcccgcgc tccggcggtt 3900

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc 3960

3901 ccgggctccg cacttcaaac cgggggcggg atgggagtgg ggccgtgtct agcgcgtgcg 3960

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt 4020

3961 ggcgctcgac tagctggtcc ttccggcgtg gcactttctc cgccgacgtg acgaaccgca 4020

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc 4080

4021 cgtagcgagc tgggacatgg cgcgtgaact cgcgtcgctc cttcactgcg ggtggctccg 4080

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc 4140

4081 gtccgccgcg ccacggaagg cactcctgcg taactggctc cggctgcggg accgccggcg 4140

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg 4200

4141 gctcttactt gcggttctcc ttgttcgtac tttggcgtgg tcctgccggt cctgcttggc 4200

.......... ORF 2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg 4260

4201 aaaaagtaat ggcttctcta gctccgcctc tactagcgcc ggcccatgca caagctcggc 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag 4320

4261 gggcgcgtgc agagttggca cgccgacgta ctttaggacc ggccaaacag actacggttc 4320

.......... .......... .......... .......... .......... ..........

.......... SfiI(4334) .......... .......... .......... ..........

4321 ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg 4380

4321 gaccgccgga ccggccggtc gaaccggcga cttctttggc tcgcggcggc agatttttcc 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga 4440

4381 actacacata aactcatttt gtcgaacgca gtacgccagc gacgcatata ctacgctact 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag 4500

4441 catttatttg tttatgcgtt ccccttgcgt acttccaata gcgacatgaa ttggtctttc 4500

.......... .......... .......... .......... ORF 5 ..........

.......... .......... .......... .......... .......... ..........

4501 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg 4560

4501 cgcccagtcc gttctgctgg tagcgttggg tagatcgggc gcgggacgtt gagcggcccc 4560

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... EagI(4613)

4561 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc 4620

4561 ggctacaaga caatcagcta aggctagggg tcccgtcacg ggcgctaacc cgccggcacg 4620

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga 4680

4621 cccttctagt tggcgattgg caacagccgt agctggcggg ctgctaactg gcgctgcact 4680

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg 4740

4681 tccggtagcc ggccgcgctg aagcatcact agctgcctcg cggggtccgc cgcctgaacc 4740

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg 4800

4741 gacacaggcg ctagttccgt cggctgaagc acgactaagg ccacgtcggt tcgggaatgc 4800

.......... .......... ORF 5 .......... .......... ..........

NdeI(4804) .......... .......... .......... .......... ..........

4801 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg 4860

4801 tgtatacccg gtggcggctg gaccacctcg accaattcgt cgcgtaactc cagtgcctac 4860

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg 4920

4861 cttccgatgt tcgccggaaa cagcacagcg cccgctagtt tccgtgcgcg tagccgccac 4920

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc 4980

4921 tccaacggct ccgcgaccgg cccatgctcg acgggtaaga actcagggca tagtgcgtcg 4980

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg 5040

4981 cgcactcgat gggtccgtga cggcggcggc cgtgttggca agaacttagt cttgggctcc 5040

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag 5100

5041 cgctgcgacg ggcgctccag gtccgcgacc ggcgacttta atttagtttt gagtaaactc 5100

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... EagI(5150) ..........

5101 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag 5160

5101 aattactcca tttctctttt actcgttttc gtgtttgtgc gattcacggc cggcaggctc 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg 5220

5161 gcgtgcgtcg tcgttccgac gttgcaaccg gtcggaccgt ctgtgcggtc ggtacttcgc 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca 5280

5221 ccagttgaaa gtcaacggcc gcctcctagt gtggttcgac ttctacatgc gccatgcggt 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag 5340

5281 tccgttctgg taatggctcg acgatagact tatgtagcgc gtcgatggtc tcatttactc 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag 5400

5341 gtttacttat ttactcatct acttaaaatc gccgatttcc tccgccgtac cttttagttc 5400

.......... .......... .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

5401 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag 5460

5401 ttgttggtcc gtggctgcgg caccttacgg ggtacacacc tccttgcccg ccaaccggtc 5460

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg 5520

5461 cgcattcgcc gacccaacag acggccggga cgttaccgtg accttggggg ttcgggctcc 5520

.......... .......... ORF 1 .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

5521 aatcggcgtg acggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat 5580

5521 ttagccgcac tgccagcgtt tggtaggccg ggccatgttt agccgcgccg cgacccacta 5580

.......... ......... ORF 1 ORF 1 ......... .......... ..........

SexAI(5583) ........ .......... .......... .......... ..........

5581 gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa 5640

5581 ctggaccacc tcttcaactt ccggcgcgtc cggcgggtcg ccgttgcgta gctccgtctt 5640

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa 5700

5641 cgtgcggggc cacttagcac cgttcgccgg cgactagctt aggcgtttct tagggccgtt 5700

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat 5760

5701 ggcggccgtc ggccacgcgg cagctaatcc ttcggcgggt tcccgctgct cgttggtcta 5760

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg 5820

5761 aaaaagcaag gctacgagat actgcacccg tgggcgctat cagcgtcgta gtacctgcac 5820

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt 5880

5821 cggcaaaagg cagacagctt cgcactggct gctcgaccgc tccactaggc gatgctcgaa 5880

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... EagI(5913) .......... ..........

5881 ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac 5940

5881 ggtctgcccg tgcatctcca aaggcgtccc ggccggccgt accggtcaca caccctaatg 5940

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg 6000

5941 ctggaccatg actaccgcca aagggtagat tggcttaggt acttggctat ggcccttccc 6000

.......... ORF 1 ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc 6060

6001 ttccctctgt tcgggccggc gcacaaggca ggtgtgcaac gcctgcatga gttcaagacg 6060

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac 6120

6061 gccgctcggc taccgccttt cgtctttctg ctggaccatc tttggacgta agccaatttg 6120

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta 6180

6121 tggtgcgtgc aacggtacgt cgcatgcttc ttccggttct tgccggcgga ccactgccat 6180

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg 6240

6181 aggctcccac ttcggaacta atcggcgatg ttctagcatt tctcgctttg gcccgccggc 6240

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac 6300

6241 ctcatgtagc tctagctcga tcgactaacc tacatggcgc tctagtgtct tccgttcttg 6300

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... ClaI(6339) .......... EagI(6352)

6301 ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt 6360

6301 ggcctgcacg actgccaagt ggggctaatg aaaaactagc tagggccgta gccggcaaaa 6360

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg 6420

6361 gagatggcgg accgtgcggc gcggcgtccg ttccgtcttc ggtctaccaa caagttctgc 6420

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag 6480

6421 tagatgcttg cgtcaccgtc gcggcctctc aagttcttca agacaaagtg gcacgcgttc 6480

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc 6540

6481 gactagccca gtttactgga cggcctcatg ctaaacttcc tcctccgccc cgtccgaccg 6540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa 6600

6541 ggctaggatc agtacgcgat ggcgttggac tagctcccgc ttcgtaggcg gccaaggatt 6600

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc 6660

6601 acatgcctcg tctacgatcc cgtttaacgg gatcgtcccc tttttccagc ttttccagag 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg 6720

6661 aaaggacacc tatcgtgcat gtaacccttg ggtttcggca tgtaaccctt ggccttgggc 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata 6780

6721 atgtaaccct tgggtttcgg catgtaaccc ttggccagtg tgtacattca ctgactatat 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa 6840

6781 tttctctttt ttccgctaaa aaggcggatt ttgagaaatt ttgaataatt ttgagaattt 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg 6900

6841 tgggcggacc ggacacgtat tgacagaccg gtcgcgtgtc ggcttctcga cgtttttcgc 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc 6960

6901 ggatgggaag ccagcgacgc gagggatgcg gggcggcgaa gcgcagccgg atagcgccgg 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc 7020

6961 cgaccggcga gtttttaccg accggatgcc ggtccgttag atggtcccgc gcctgttcgg 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg 7080

7021 cgcggcagcg gtgagctggc ggccgcgggt gtagttccgt gggacggagc gcgcaaagcc 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 7140

7081 actactgcca cttttggaga ctgtgtacgt cgagggcctc tgccagtgtc gaacagacat 7140

.......... ........ pGEX_3_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7141 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 7200

7141 tcgcctacgg ccctcgtctg ttcgggcagt cccgcgcagt cgcccacaac cgcccacagc 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg 7260

7201 cccgcgtcgg tactgggtca gtgcatcgct atcgcctcac atatgaccga attgatacgc 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NdeI(7293) .......... ..........

7261 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc 7320

7261 cgtagtctcg tctaacatga ctctcacgtg gtatacgcca cactttatgg cgtgtctacg 7320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc 7380

7321 cattcctctt ttatggcgta gtccgcgaga aggcgaagga gcgagtgact gagcgacgcg 7380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 7440

7381 agccagcaag ccgacgccgc tcgccatagt cgagtgagtt tccgccatta tgccaatagg 7440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 7500

7441 tgtcttagtc ccctattgcg tcctttcttg tacactcgtt ttccggtcgt tttccggtcc 7500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 7560

7501 ttggcatttt tccggcgcaa cgaccgcaaa aaggtatccg aggcgggggg actgctcgta 7560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 7620

7561 gtgtttttag ctgcgagttc agtctccacc gctttgggct gtcctgatat ttctatggtc 7620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 7680

7621 cgcaaagggg gaccttcgag ggagcacgcg agaggacaag gctgggacgg cgaatggcct 7680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 7740

7681 atggacaggc ggaaagaggg aagcccttcg caccgcgaaa gagtatcgag tgcgacatcc 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 7800

7741 atagagtcaa gccacatcca gcaagcgagg ttcgacccga cacacgtgct tggggggcaa 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 7860

7801 gtcgggctgg cgacgcggaa taggccattg atagcagaac tcaggttggg ccattctgtg 7860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 7920

7861 ctgaatagcg gtgaccgtcg tcggtgacca ttgtcctaat cgtctcgctc catacatccg 7920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 7980

7921 ccacgatgtc tcaagaactt caccaccgga ttgatgccga tgtgatcttc ctgtcataaa 7980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 8040

7981 ccatagacgc gagacgactt cggtcaatgg aagccttttt ctcaaccatc gagaactagg 8040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 8100

8041 ccgtttgttt ggtggcgacc atcgccacca aaaaaacaaa cgttcgtcgt ctaatgcgcg 8100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 8160

8101 tctttttttc ctagagttct tctaggaaac tagaaaagat gccccagact gcgagtcacc 8160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 aacgaaaact cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc 8220

8161 ttgcttttga gtgcaattcc ctaaaaccag tacgtaagat ccatgatttt gttaagtagg 8220

.......... .......... .......... .......... ......... KanR1 ORF 2

.......... .......... .......... .......... .......... ..........

8221 agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat 8280

8221 tcattttata ttataaaata aaagagggtt agtccgaact aggggtcatt cagtttttta 8280

.......... ......... KanR1 ORF 2 ......... .......... ..........

.......... ......... EcoRV(8307) ......... .......... ..........

8281 agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg 8340

8281 tcgagctgta tgacaagaag gggctatagg agggactagc tggcctgcgt cttccgttac 8340

.......... ......... KanR1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca 8400

8341 agtatggtga acaggcggga cggcgaagag ggttctagtt atttcggtga atgaaacggt 8400

.......... ......... KanR1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg 8460

8401 agaaagtgtt tctacaacga cagagggtcc agcggcaccc ttttctgttc aaggagaagc 8460

.......... ......... KanR1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct 8520

8461 ccgaaaaggc agaaattttt tagtatgtcg agcgcgccta gaaatttacc tcacagaaga 8520

.......... ......... KanR1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8521 tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct 8580

8521 agggtcaaaa gcgttaggtg tagccggtct agcaataagt cattcattag gttaagccga 8580

.......... ......... KanR1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8581 aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc 8640

8581 ttcgccgaca gattcgataa gcatatccct gttaggctat acagctacct cactttctcg 8640

.......... ......... KanR1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8641 ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct 8700

8641 gactacgtga ggcgtatgtc gagctattag aaaagtcccg aaacaagtag aagtatgaga 8700

.......... ......... KanR1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8701 tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt 8760

8701 aggctcgttt cctgcggtag ccggagtgag tactcgtcta acgaggtcgg tagtacggca 8760

.......... ......... KanR1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8761 tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt 8820

8761 agtttcacgt cctggaaacc ttgtccgtcg aaaggaaggt cggtatcgta gtacaggaaa 8820

.......... ......... KanR1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8821 tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg 8880

8821 agggcaaggt gtagtatcca ccagggaaat atggccgaca ggcagtaaaa atttatatcc 8880

.......... ......... KanR1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8881 ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt 8940

8881 aaaagtaaaa gagggtggtc gaatatatgg aatcgtcctc tgtaaggaag gcatagaaaa 8940

.......... ......... KanR1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8941 acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat tttagccatt 9000

8941 tgcgtcgcca taaaaagcta gtcaaaaaag ttaaggccac tataagagta aaatcggtaa 9000

.......... ......... KanR1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9001 tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca 9060

9001 ataataaagg aaggagaaaa gatgtcataa atttctatgg ggttcttcga ttaatattgt 9060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9061 agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt 9120

9061 tctgcttgag gttaagtgac aaggaacgta agattttgga atttatggtc ttttgtcgaa 9120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9121 tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac 9180

9121 aaagtttcaa caaaagtttc aaccgcatat tgtatcatag ctgcctcggc taaaactttg 9180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg 9240

9181 gcgccactag tgtccgtcgt tgcgagacag tagcaatgtt agttgtacga tgggaggcgc 9240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa 9300

9241 tctagtaggc acaaagtttg ggccgtcgaa tcaacggcaa gaaggcttat cgtagccatt 9300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9301 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg 9360

9301 gtactcgttt cagacggcgg aatgttgccg agagggcgac tgcggcaggg cctgactacc 9360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9361 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc 9420

9361 cgacggacat agctcaccac taaaacacgg ctcgacggcc agcccctcga caaccgaccg 9420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9421 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt 9480

9421 accaccgtcc tatataacac cacatttgtt taactgcgaa tctgttgaat tattgtgtaa 9480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9481 gcggacgttt ttaatgtact gaattaacgc cgaattaatt cgggggatct ggattttagt 9540

9481 cgcctgcaaa aattacatga cttaattgcg gcttaattaa gccccctaga cctaaaatca 9540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9541 actggatttt ggttttagga attagaaatt ttattgatag aagtatttta caaatacaaa 9600

9541 tgacctaaaa ccaaaatcct taatctttaa aataactatc ttcataaaat gtttatgttt 9600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9601 tacatactaa gggtttctta tatgctcaac acatgagcga aaccctatag gaaccctaat 9660

9601 atgtatgatt cccaaagaat atacgagttg tgtactcgct ttgggatatc cttgggatta 9660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9661 tcccttatct gggaactact cacacattat tatggagaaa ctcgagcttg tcgatcgaca 9720

9661 agggaataga cccttgatga gtgtgtaata atacctcttt gagctcgaac agctagctgt 9720

.......... .......... .......... .......... ORF 3 ..........

.......... .......... .......... .......... .......... ..........

9721 gatccggtcg gcatctactc tatttctttg ccctcggacg agtgctgggg cgtcggtttc 9780

9721 ctaggccagc cgtagatgag ataaagaaac gggagcctgc tcacgacccc gcagccaaag 9780

.......... .......... ORF 3 ORF 5 .......... ..........

.......... ScaI(9795) .......... .......... EagI(9821) ..........

9781 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga 9840

9781 gtgatagccg ctcatgaaga tgtgtcggta gccaggtctg ccggcgcgaa gacgcccgct 9840

.......... ......... ORF 3 ORF 5 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9841 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg 9900

9841 aaacacatgc gggctgtcag ggccgaggcc tagcctgcta acgcagcgta gctgggacgc 9900

.......... ......... ORF 3 ORF 5 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9901 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa 9960

9901 gggttcgacg tagtagcttt aacggcagtt ggttcgagac tatctcaacc agttctggtt 9960

.......... ......... ORF 3 ORF 5 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

9961 tgcggagcat atacgcccgg agtcgtggcg atcctgcaag ctccggatgc ctccgctcga 10020

9961 acgcctcgta tatgcgggcc tcagcaccgc taggacgttc gaggcctacg gaggcgagct 10020

.......... ......... ORF 3 ORF 5 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10021 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga 10080

10021 tcatcgcgca gacgacgagg tatgttcggt tggtgccgga ggtcttcttc tacaaccgct 10080

.......... ......... ORF 3 ORF 5 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10081 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc 10140

10081 ggagcataac ccttaggggc ttgtagcgga gcgaggtcag ttactggcga caatacgccg 10140

.......... ......... ORF 3 ORF 5 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10141 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg 10200

10141 gtaacaggca gtcctgtaac aacctcggct ttaggcgcac gtgctccacg gcctgaagcc 10200

.......... ......... ORF 3 ORF 5 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10201 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg 10260

10201 ccgtcaggag ccgggtttcg tagtcgagta gctctcggac gcgctgcctg cgtgactgcc 10260

.......... ......... ORF 3 ORF 5 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10261 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat 10320

10261 acagcaggta gtgtcaaacg gtcactatgt gtacccctag tcgttagcgc gtatacttta 10320

.......... ......... ORF 3 ORF 5 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10321 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct 10380

10321 gtgcggtaca tcacataact ggctaaggaa cgccaggctt acccggcttg ggcgagcaga 10380

.......... ......... ORF 3 ORF 5 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10381 ggctaagatc ggccgcagcg atcgcatcca tagcctccgc gaccggttgt agaacagcgg 10440

10381 ccgattctag ccggcgtcgc tagcgtaggt atcggaggcg ctggccaaca tcttgtcgcc 10440

.......... ......... ORF 3 ORF 5 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

10441 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat 10500

10441 cgtcaagcca aagtccgtcc agaacgttgc actgtgggac acgtgccgcc ctctacgtta 10500

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10501 aggtcaggct ctcgctaaac tccccaatgt caagcacttc cggaatcggg agcgcggccg 10560

10501 tccagtccga gagcgatttg aggggttaca gttcgtgaag gccttagccc tcgcgccggc 10560

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10561 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc 10620

10561 tacgtttcac ggctatttgt attgctagaa acatctttgg tagccgcgtc gataaatggg 10620

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10621 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg 10680

10621 cgtcctgtat aggtgcggga ggatgtagct tcgactttcg tgctctaaga agcgggaggc 10680

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10681 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg 10740

10681 tctcgacgta gtccagcctc tgcgacagct tgaaaagcta gtctttgaag agctgtctgc 10740

.......... .......... ORF 5 .......... .......... ..........

.......... .......... .......... ... XmaI(10779),SmaI(10781) ...

10741 tcgcggtgag ttcaggcttt ttcatatctc attgcccccc gggatctgcg aaagctcgag 10800

10741 agcgccactc aagtccgaaa aagtatagag taacgggggg ccctagacgc tttcgagctc 10800

.......... ORF 5 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10801 agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa 10860

10801 tctctatcta aacatctctc tctgaccact aaagtcgcac aggagaggtt tactttactt 10860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10861 cttccttata tagaggaagg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt 10920

10861 gaaggaatat atctccttcc agaacgcttc ctatcaccct aacacgcagt agggaatgca 10920

.......... .......... .......... .......... .......... ..........

......... EcoRV(10931) ......... .......... .......... ..........

10921 cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt 10980

10921 gtcacctcta tagtgtagtt aggtgaacga aacttctgca ccaaccttgc agaagaaaaa 10980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

10981 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt 11040

10981 ggtgctacga ggagcaccca cccccaggta gaaaccctgg tgacagccgt ctccgtagaa 11040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11041 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta 11100

11041 cttgctatcg gaaaggaaat agcgttacta ccgtaaacat ccacggtgga aggaaaagat 11100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11101 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata 11160

11101 gacaggaaaa ctacttcact gtctatcgac ccgttacctt aggctcctcc aaagggctat 11160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11161 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat 11220

11161 aatgggaaac aacttttcag agttatcggg aaaccagaag actctgacat agaaactata 11220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11221 tcttggagta gacgagagtg tcgtgctcca ccatgttatc acatcaatcc acttgctttg 11280

11221 agaacctcat ctgctctcac agcacgaggt ggtacaatag tgtagttagg tgaacgaaac 11280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11281 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt 11340

11281 ttctgcacca accttgcaga agaaaaaggt gctacgagga gcacccaccc ccaggtagaa 11340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11341 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc 11400

11341 accctggtga cagccgtctc cgtagaactt gctatcggaa aggaaatagc gttactaccg 11400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11401 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc 11460

11401 taaacatcca cggtggaagg aaaagatgac aggaaaacta cttcactgtc tatcgacccg 11460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11461 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt 11520

11461 ttaccttagg ctcctccaaa gggctataat gggaaacaac ttttcagagt tatcgggaaa 11520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11521 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca 11580

11521 ccagaagact ctgacataga aactataaga acctcatctg ctctcacagc acgaggtggt 11580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11581 tgttggcaag ctgctctagc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 11640

11581 acaaccgttc gacgagatcg gttatgcgtt tggcggagag gggcgcgcaa ccggctaagt 11640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11641 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 11700

11641 aattacgtcg accgtgctgt ccaaagggct gacctttcgc ccgtcactcg cgttgcgtta 11700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11701 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 11760

11701 attacactca atcgagtgag taatccgtgg ggtccgaaat gtgaaatacg aaggccgagc 11760

.......... .......... .......... ......... lac_promoter .........

.......... .......... .......... .......... .......... ..........

11761 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 11820

11761 atacaacaca ccttaacact cgcctattgt taaagtgtgt cctttgtcga tactggtact 11820

.......... .......... .. M13_pUC_rev_primer M13_reverse_primer ..

EcoRI(11826) . SacI(11836),EcoRI(11841) ... .......... ..........

11821 ttacgaattc gagctctgag aattctcagc gtggtgcggc tgaggtggct gggctcgagc 11880

11821 aatgcttaag ctcgagactc ttaagagtcg caccacgccg actccaccga cccgagctcg 11880

.......... .......... .......... .......... ORF 4 ..........

.......... .......... .......... .......... .......... ..........

11881 cctttggagt ggtgtacacg tccagcaggg tgacgtcagc cacaccggag ctttgccacc 11940

11881 ggaaacctca ccacatgtgc aggtcgtccc actgcagtcg gtgtggcctc gaaacggtgg 11940

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

11941 cctcggcgtt cagtgccacc tcgtagaggt tgccgatacc ccagccttgc ttggcccact 12000

11941 ggagccgcaa gtcacggtgg agcatctcca acggctatgg ggtcggaacg aaccgggtga 12000

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12001 ccttgaagtg gtcggagacg gtgatgtgcc cggaggtcct cttctgttgc ctgacggaga 12060

12001 ggaacttcac cagcctctgc cactacacgg gcctccagga gaagacaacg gactgcctct 12060

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12061 agtattgctt gaaggtctcg ctgccaccgt tgatggttgg gccagtgtgg tccatctgga 12120

12061 tcataacgaa cttccagagc gacggtggca actaccaacc cggtcacacc aggtagacct 12120

.......... .......... ORF 4 .......... .......... ..........

BglII(12122) ........ .......... .......... .......... ..........

12121 agatcttgta ctgtgccccg tcgatggtga ccatcttgcc ttgtgcgtct ggcacccagt 12180

12121 tctagaacat gacacggggc agctaccact ggtagaacgg aacacgcaga ccgtgggtca 12180

.......... .......... ORF 4 .......... .......... ..........

.......... ......... ScaI(12205) ......... .......... ..........

12181 cgacccagtc ctcgatgatg tagtactcga cgagtggcac gccgttcaga cccctgttct 12240

12181 gctgggtcag gagctactac atcatgagct gctcaccgtg cggcaagtct ggggacaaga 12240

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12241 ggaaccagcc gtacacgcag agcctggagt tgccgcttgc gctggcggtc tgcttgtagg 12300

12241 ccttggtcgg catgtgcgtc tcggacctca acggcgaacg cgaccgccag acgaacatcc 12300

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12301 tggctgcgta gtccaggccg atgtagtcgt agtcggtggc cttcttttgg ctcccgaagt 12360

12301 accgacgcat caggtccggc tacatcagca tcagccaccg gaagaaaacc gagggcttca 12360

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12361 cgaggcccct ccgtgccagg aagttgccgc ggttcacggc tgcgttccac tctgccttga 12420

12361 gctccgggga ggcacggtcc ttcaacggcg ccaagtgccg acgcaaggtg agacggaact 12420

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12421 acgttgcgcc gctaccgagg gtcatggagc cgttgccacc ggtgttgtcg agccaaatct 12480

12421 tgcaacgcgg cgatggctcc cagtacctcg gcaacggtgg ccacaacagc tcggtttaga 12480

.......... .......... ORF 4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

12481 cgtagctgaa gccgtcgttg acgcccttgt gttggttttg gccgttcccg acggtgaact 12540

12481 gcatcgactt cggcagcaac tgcgggaaca caaccaaaac cggcaagggc tgccacttga 12540

.......... .......... ORF 4 .......... .......... ..........

......... NheI(12552) ......... .......... .......... ..........

12541 tcttcccgtt gctagccatt gtttattgct cttgcttgct ttaggatg.. .......... 12588

12541 agaagggcaa cgatcggtaa caaataacga gaacgaacga aatcctac.. .......... 12588

ORF 4 .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-12588 Reference author: Medi V.G., Bhat R.S., Kuruvinashetti M.S.; Reference title: "Promoter-probe vector with synthetic xylanase reporter"; Reference location: Unpublished.
Reference number: [2] Reference positions: 1-12588 Reference author: Medi V.G., Bhat R.S., Kuruvinashetti M.S.; Reference title: ; Reference location: Submitted (20-MAY-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Department of Biotechnology, University of Agricultural Sciences, Dharwad, Reference location: Karnataka 580 005, India