Sequence of pbgal-Basic

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pbgal-Basic

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pbgal-Basic : 7482 BP; 1860 A; 1863 C; 1888 G; 1871 T; 0 other;

SmaI KpnI NheI(28),XhoI(33),BglII(37) HindIII(47)

1 cccgggaggt accgagctct tacgcgtgct agctcgagat ctaagtaagc ttgggggatc 60

1 gggccctcca tggctcgaga atgcgcacga tcgagctcta gattcattcg aaccccctag 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gaaagagcct gctaaagcaa aaaagaagtc accatgtcgt ttactttgac caacaagaac 120

61 ctttctcgga cgatttcgtt ttttcttcag tggtacagca aatgaaactg gttgttcttg 120

.......... .......... .......... .......... lacZ ORF 1 ..........

.......... .......... .......... .......... .......... ..........

121 gtgattttcg ttgccggtct gggaggcatt ggtctggaca ccagcaagga gctgctcaag 180

121 cactaaaagc aacggccaga ccctccgtaa ccagacctgt ggtcgttcct cgacgagttc 180

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 cgcgatcccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 240

181 gcgctagggc agcaaaatgt tgcagcactg acccttttgg gaccgcaatg ggttgaatta 240

....... M13_pUC_fwd_pri lacZ ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

241 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 300

241 gcggaacgtc gtgtaggggg aaagcggtcg accgcattat cgcttctccg ggcgtggcta 300

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca 360

301 gcgggaaggg ttgtcaacgc gtcggactta ccgcttaccg cgaaacggac caaaggccgt 360

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc 420

361 ggtcttcgcc acggcctttc gaccgacctc acgctagaag gactccggct atgacagcag 420

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtaacctat 480

421 caggggagtt tgaccgtcta cgtgccaatg ctacgcgggt agatgtggtt gcattggata 480

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc 540

481 gggtaatgcc agttaggcgg caaacaaggg tgcctcttag gctgcccaac aatgagcgag 540

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc 600

541 tgtaaattac aactactttc gaccgatgtc cttccggtct gcgcttaata aaaactaccg 600

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagt 660

601 caattgagcc gcaaagtaga caccacgttg cccgcgaccc agccaatgcc ggtcctgtca 660

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg 720

661 gcaaacggca gacttaaact ggactcgcgt aaaaatgcgc ggcctctttt ggcggagcgc 720

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gtgatggtgc tgcgttggag tgacggcagt tatctggaag atcaggatat gtggcggatg 780

721 cactaccacg acgcaacctc actgccgtca atagaccttc tagtcctata caccgcctac 780

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... AatII(800) .......... .......... .......... ..........

781 agcggcattt tccgtgacgt ctcgttgctg cataaaccga ctacacaaat cagcgatttc 840

781 tcgccgtaaa aggcactgca gagcaacgac gtatttggct gatgtgttta gtcgctaaag 840

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 catgttgcca ctcgctttaa tgatgatttc agccgcgctg tactggaggc tgaagttcag 900

841 gtacaacggt gagcgaaatt actactaaag tcggcgcgac atgacctccg acttcaagtc 900

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 atgtgcggcg agttgcgtga ctacctacgg gtaacagttt ctttatggca gggtgaaacg 960

901 tacacgccgc tcaacgcact gatggatgcc cattgtcaaa gaaataccgt cccactttgc 960

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat 1020

961 gtccagcggt cgccgtggcg cggaaagccg ccactttaat agctactcgc accaccaata 1020

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gccgatcgcg tcacactacg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc 1080

1021 cggctagcgc agtgtgatgc agacttgcag cttttgggct ttgacacctc gcggctttag 1080

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ccgaatctct atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca 1140

1081 ggcttagaga tagcacgcca ccaacttgac gtgtggcggc tgccgtgcga ctaacttcgt 1140

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac 1200

1141 cttcggacgc tacagccaaa ggcgctccac gcctaacttt taccagacga cgacgacttg 1200

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc atcatcctct gcatggtcag 1260

1201 ccgttcggca acgactaagc tccgcaattg gcagtgctcg tagtaggaga cgtaccagtc 1260

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... ......... EcoRV(1290) ......... .......... ..........

1261 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac 1320

1261 cagtacctac tcgtctgcta ccacgtccta taggacgact acttcgtctt gttgaaattg 1320

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac 1380

1321 cggcacgcga caagcgtaat aggcttggta ggcgacacca tgtgcgacac gctggcgatg 1380

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt 1440

1381 ccggacatac accacctact tcggttataa ctttgggtgc cgtaccacgg ttacttagca 1440

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctgaccgatg atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag 1500

1441 gactggctac taggcgcgac cgatggccgc tactcgcttg cgcattgcgc ttaccacgtc 1500

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... BclI(1523) .......... .......... ..........

1501 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc 1560

1501 gcgctagcat tagtgggctc acactagtag accagcgacc ccttacttag tccggtgccg 1560

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gctaatcacg acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag 1620

1561 cgattagtgc tgcgcgacat agcgacctag tttagacagc taggaagggc gggccacgtc 1620

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc 1680

1621 atacttccgc cgcctcggct gtggtgccgg tggctataat aaacgggcta catgcgcgcg 1680

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg 1740

1681 cacctacttc tggtcgggaa gggccgacac ggctttacca ggtagttttt taccgaaagc 1740

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt 1800

1741 gatggacctc tctgcgcggg cgactaggaa acgcttatgc gggtgcgcta cccattgtca 1800

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt atccccgttt acagggcggc 1860

1801 gaaccgccaa agcgatttat gaccgtccgc aaagcagtca taggggcaaa tgtcccgccg 1860

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg 1920

1861 aagcagaccc tgacccacct agtcagcgac taatttatac tacttttgcc gttgggcacc 1920

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tcggcttacg gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt 1980

1921 agccgaatgc cgccactaaa accgctatgc ggcttgctag cggtcaagac atacttgcca 1980

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag 2040

1981 gaccagaaac ggctggcgtg cggcgtaggt cgcgactgcc ttcgttttgt ggtcgtcgtc 2040

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt 2100

2041 aaaaaggtca aggcaaatag gcccgtttgg tagcttcact ggtcgcttat ggacaaggca 2100

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 catagcgata acgagctcct gcactggatg gtggcgctgg atggtaagcc gctggcaagc 2160

2101 gtatcgctat tgctcgagga cgtgacctac caccgcgacc taccattcgg cgaccgttcg 2160

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta 2220

2161 ccacttcacg gagacctaca gcgaggtgtt ccatttgtca actaacttga cggacttgat 2220

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ccgcagccgg agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg 2280

2221 ggcgtcggcc tctcgcggcc cgttgagacc gagtgtcatg cgcatcacgt tggcttgcgc 2280

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 accgcatggt cagaagccgg gcacatcagc gcctggcagc agtggcgtct ggcggaaaac 2340

2281 tggcgtacca gtcttcggcc cgtgtagtcg cggaccgtcg tcaccgcaga ccgccttttg 2340

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc atctgaccac cagcgaaatg 2400

2341 gagtcacact gcgaggggcg gcgcagggtg cggtagggcg tagactggtg gtcgctttac 2400

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gatttttgca tcgagctggg taataagcgt tggcaattta accgccagtc aggctttctt 2460

2401 ctaaaaacgt agctcgaccc attattcgca accgttaaat tggcggtcag tccgaaagaa 2460

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tcacagatgt ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc 2520

2461 agtgtctaca cctaaccgct attttttgtt gacgactgcg gcgacgcgct agtcaagtgg 2520

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc 2580

2521 gcacgtggcg acctattgct gtaaccgcat tcacttcgct gggcgtaact gggattgcgg 2580

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc 2640

2581 acccagcttg cgaccttccg ccgcccggta atggtccggc ttcgtcgcaa caacgtcacg 2640

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 acggcagata cacttgctga tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag 2700

2641 tgccgtctat gtgaacgact acgccacgac taatgctggc gagtgcgcac cgtcgtagtc 2700

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gggaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg 2760

2701 cccttttgga ataaatagtc ggccttttgg atggcctaac taccatcacc agtttaccgc 2760

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 attaccgttg atgttgaagt ggcgagcgat acaccgcatc cggcgcggat tggcctgaac 2820

2761 taatggcaac tacaacttca ccgctcgcta tgtggcgtag gccgcgccta accggacttg 2820

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tgccagctgg cgcaggtagc agagcgggta aactggctcg gattagggcc gcaagaaaac 2880

2821 acggtcgacc gcgtccatcg tctcgcccat ttgaccgagc ctaatcccgg cgttcttttg 2880

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg 2940

2881 atagggctgg cggaatgacg gcggacaaaa ctggcgaccc tagacggtaa cagtctgtac 2940

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat 3000

2941 atatggggca tgcagaaggg ctcgcttttg ccagacgcga cgccctgcgc gcttaactta 3000

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tatggcccac accagtggcg cggcgacttc cagttcaaca tcagccgcta cagtcaacag 3060

3001 ataccgggtg tggtcaccgc gccgctgaag gtcaagttgt agtcggcgat gtcagttgtc 3060

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat 3120

3061 gttgactacc tttggtcggt agcggtagac gacgtgcgcc ttcttccgtg taccgactta 3120

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... NdeI(3134) .......... .......... .......... ..........

3121 atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg 3180

3121 tagctgccaa aggtataccc ctaaccaccg ctgctgagga cctcgggcag tcatagccgc 3180

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gaattacagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa 3240

3181 cttaatgtcg actcgcggcc agcgatggta atggtcaacc agaccacagt ttttattatt 3240

.......... .......... lacZ ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta 3300

3241 attggcccgt ccggtacaga cgggcataaa gcgcattcct ttaggtaata catgataaat 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tcaggtggcc cccgctgaat tggaatcgat attgttacaa caccccaaca tcttcgacgc 3360

3301 agtccaccgg gggcgactta accttagcta taacaatgtt gtggggttgt agaagctgcg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gggcgtggca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt 3420

3361 cccgcaccgt ccagaagggc tgctactgcg gccacttgaa gggcggcggc aacaacaaaa 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac 3480

3421 cctcgtgcct ttctgctact gcctttttct ctagcaccta atgcagcggt cagttcattg 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac 3540

3481 ttggcgcttt ttcaacgcgc ctcctcaaca caaacacctg cttcatggct ttccagaatg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa 3600

3541 gccttttgag ctgcgttctt tttagtctct ctaggagtat ttccggttct tcccgccttt 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gtccaaattg taaaatgtaa ctgtattcag cgatgacgaa attcttagct attgtaatac 3660

3601 caggtttaac attttacatt gacataagtc gctactgctt taagaatcga taacattatg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg 3720

3661 acgctactca ccgtcccgcc ccgcattaaa aaaattccgt caataaccac gggaatttgc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cctggtgcta cgcctgaata agtgataata agcggatgaa tggcagaaat tcgccggatc 3780

3721 ggaccacgat gcggacttat tcactattat tcgcctactt accgtcttta agcggcctag 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt 3840

3781 aaacacttcc ttggaatgaa gacaccacac tgtattaacc tgtttgatgg atgtctctaa 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta 3900

3841 atttcgagat tccatttata ttttaaaaat tcacatatta cacaatttga tgactaagat 3900

.......... SV40_int .......... ........ SV40_3_splice ........

.......... .......... .......... .......... .......... ..........

3901 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat 3960

3901 taacaaacac ataaaatcta aggttggata ccttgactac ttaccctcgt caccacctta 3960

SV40_3_splice ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct 4020

3961 cggaaattac tccttttgga caaaacgagt cttctttacg gtagatcact actactccga 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag 4080

4021 tgacgactga gagttgtaag atgaggaggt tttttcttct ctttccatct tctggggttc 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt 4140

4081 ctgaaaggaa gtcttaacga ttcaaaaaac tcagtacgac acaaatcatt atcttgagaa 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg 4200

4141 cgaacgaaac gataaatgtg gtgtttcctt tttcgacgtg acgatatgtt cttttaatac 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt 4260

4201 ctttttataa gacattggaa atattcatcc gtattgtcaa tattagtatt gtatgacaaa 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt 4320

4261 aaagaatgag gtgtgtccgt atctcacaga cgataattat tgatacgagt ttttaacaca 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg 4380

4321 tggaaatcga aaaattaaac atttccccaa ttattcctta taaactacat atcacggaac 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct 4440

4381 tgatctctag tattagtcgg tatggtgtaa acatctccaa aatgaacgaa attttttgga 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt 4500

4441 gggtgtggag ggggacttgg actttgtatt ttacttacgt taacaacaac aattgaacaa 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 4560

4501 ataacgtcga atattaccaa tgtttatttc gttatcgtag tgtttaaagt gtttatttcg 4560

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 4620

4561 taaaaaaagt gacgtaagat caacaccaaa caggtttgag tagttacata gaatagtaca 4620

.......... ........ SV40_PA_term EBV_rev_pri ........ ..........

BamHI(4623) SalI(4629) ........ .......... .......... ..........

4621 ctggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc 4680

4621 gacctaggca gctggctacg ggaactctcg gaagttgggt cagtcgagga aggccacccg 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg 4740

4681 cgccccgtac tgatagcagc ggcgtgaata ctgacagaag aaatagtacg ttgagcatcc 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 4800

4741 tgtccacggc cgtcgcgaga aggcgaagga gcgagtgact gagcgacgcg agccagcaag 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 4860

4801 ccgacgccgc tcgccatagt cgagtgagtt tccgccatta tgccaatagg tgtcttagtc 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 4920

4861 ccctattgcg tcctttcttg tacactcgtt ttccggtcgt tttccggtcc ttggcatttt 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 4980

4921 tccggcgcaa cgaccgcaaa aaggtatccg aggcgggggg actgctcgta gtgtttttag 4980

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

4981 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 5040

4981 ctgcgagttc agtctccacc gctttgggct gtcctgatat ttctatggtc cgcaaagggg 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 5100

5041 gaccttcgag ggagcacgcg agaggacaag gctgggacgg cgaatggcct atggacaggc 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 5160

5101 ggaaagaggg aagcccttcg caccgcgaaa gagtatcgag tgcgacatcc atagagtcaa 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 5220

5161 gccacatcca gcaagcgagg ttcgacccga cacacgtgct tggggggcaa gtcgggctgg 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 5280

5221 cgacgcggaa taggccattg atagcagaac tcaggttggg ccattctgtg ctgaatagcg 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 5340

5281 gtgaccgtcg tcggtgacca ttgtcctaat cgtctcgctc catacatccg ccacgatgtc 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 5400

5341 tcaagaactt caccaccgga ttgatgccga tgtgatcttc ctgtcataaa ccatagacgc 5400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 5460

5401 gagacgactt cggtcaatgg aagccttttt ctcaaccatc gagaactagg ccgtttgttt 5460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 5520

5461 ggtggcgacc atcgccacca aaaaaacaaa cgttcgtcgt ctaatgcgcg tctttttttc 5520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 5580

5521 ctagagttct tctaggaaac tagaaaagat gccccagact gcgagtcacc ttgcttttga 5580

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 5640

5581 gtgcaattcc ctaaaaccag tactctaata gtttttccta gaagtggatc taggaaaatt 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 5700

5641 taatttttac ttcaaaattt agttagattt catatatact catttgaacc agactgtcaa 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 5760

5701 tggttacgaa ttagtcactc cgtggataga gtcgctagac agataaagca agtaggtatc 5760

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 5820

5761 aacggactga ggggcagcac atctattgat gctatgccct cccgaatggt agaccggggt 5820

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 5880

5821 cacgacgtta ctatggcgct ctgggtgcga gtggccgagg tctaaatagt cgttatttgg 5880

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 5940

5881 tcggtcggcc ttcccggctc gcgtcttcac caggacgttg aaataggcgg aggtaggtca 5940

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 6000

5941 gataattaac aacggccctt cgatctcatt catcaagcgg tcaattatca aacgcgttgc 6000

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 6060

6001 aacaacggta acgatgtccg tagcaccaca gtgcgagcag caaaccatac cgaagtaagt 6060

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 6120

6061 cgaggccaag ggttgctagt tccgctcaat gtactagggg gtacaacacg ttttttcgcc 6120

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 6180

6121 aatcgaggaa gccaggaggc tagcaacagt cttcattcaa ccggcgtcac aatagtgagt 6180

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 6240

6181 accaataccg tcgtgacgta ttaagagaat gacagtacgg taggcattct acgaaaagac 6240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 6300

6241 actgaccact catgagttgg ttcagtaaga ctcttatcac atacgccgct ggctcaacga 6300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 6360

6301 gaacgggccg cagttatgcc ctattatggc gcggtgtatc gtcttgaaat tttcacgagt 6360

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 6420

6361 agtaaccttt tgcaagaagc cccgcttttg agagttccta gaatggcgac aactctaggt 6420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 6480

6421 caagctacat tgggtgagca cgtgggttga ctagaagtcg tagaaaatga aagtggtcgc 6480

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 6540

6481 aaagacccac tcgtttttgt ccttccgttt tacggcgttt tttcccttat tcccgctgtg 6540

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 6600

6541 cctttacaac ttatgagtat gagaaggaaa aagttataat aacttcgtaa atagtcccaa 6600

ORF 2 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 6660

6601 taacagagta ctcgcctatg tataaactta cataaatctt tttatttgtt tatccccaag 6660

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg 6720

6661 gcgcgtgtaa aggggctttt cacggtggac tgcgcgggac atcgccgcgt aattcgcgcc 6720

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

6721 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 6780

6721 gcccacacca ccaatgcgcg tcgcactggc gatgtgaacg gtcgcgggat cgcgggcgag 6780

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 6840

6781 gaaagcgaaa gaagggaagg aaagagcggt gcaagcggcc gaaaggggca gttcgagatt 6840

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 6900

6841 tagcccccga gggaaatccc aaggctaaat cacgaaatgc cgtggagctg gggttttttg 6900

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 6960

6901 aactaatccc actaccaagt gcatcacccg gtagcgggac tatctgccaa aaagcgggaa 6960

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 7020

6961 actgcaacct caggtgcaag aaattatcac ctgagaacaa ggtttgacct tgttgtgagt 7020

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 7080

7021 tgggatagag ccagataaga aaactaaata ttccctaaaa cggctaaagc cggataacca 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta 7140

7081 attttttact cgactaaatt gtttttaaat tgcgcttaaa attgttttat aattgcaaat 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 caatttccca ttcgccattc aggctacgca actgttggga agggcgatcg gtgcgggcct 7200

7141 gttaaagggt aagcggtaag tccgatgcgt tgacaaccct tcccgctagc cacgcccgga 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag gtacgtggag 7260

7201 gaagcgataa tgcggtcggg ttcgatggta ctattcattc attataattc catgcacctc 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat 7320

7261 caaaatgaac gaaatttttt ggagggtgtg gagggggact tggactttgt attttactta 7320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 7380

7321 cgttaacaac aacaattgaa caaataacgt cgaatattac caatgtttat ttcgttatcg 7380

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

7381 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 7440

7381 tagtgtttaa agtgtttatt tcgtaaaaaa agtgacgtaa gatcaacacc aaacaggttt 7440

.......... ......... SV40_PA_term ......... ......... EBV_rev_pri

.......... .......... .......... .......... .......... ..........

7441 ctcatcaatg tatcttatgg tactgtaact gagctaacat aa........ .......... 7482

7441 gagtagttac atagaatacc atgacattga ctcgattgta tt........ .......... 7482

SV40_PA_term ........ .......... .......... .......... ..........