Sequence of pcDNA3.1(+)_myc-His

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pcDNA3.1(+)_myc-His_A

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pcDNA3.1(+)/myc-His A : 5493 BP; 1273 A; 1428 C; 1398 G; 1394 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatca 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

HindIII(902) KpnI(912) BamHI(920) ........ EcoRI(943) ..........

901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca 960

901 attcgaacca tggctcgagc ctaggtgatc aggtcacacc accttaagac gtctataggt 960

.......... .......... .......... .......... .......... ..........

NotI(970) XhoI(976) XbaI(982) .......... .......... ..........

961 gcacagtggc ggccgctcga gtctagaggg cccttcgaac aaaaactcat ctcagaagag 1020

961 cgtgtcaccg ccggcgagct cagatctccc gggaagcttg tttttgagta gagtcttctc 1020

.......... .......... .......... .......... c_myc ..........

.......... AgeI(1036) .......... .......... .......... ..........

1021 gatctgaata tgcataccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca 1080

1021 ctagacttat acgtatggcc agtagtagtg gtagtggtaa ctcaaatttg ggcgactagt 1080

.......... .......... 6xHis .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 1140

1081 cggagctgac acggaagatc aacggtcggt agacaacaaa cggggagggg gcacggaagg 1140

BGH_rev_pri ........ ......... bGH_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

1141 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 1200

1141 aactgggacc ttccacggtg agggtgacag gaaaggatta ttttactcct ttaacgtagc 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 1260

1201 gtaacagact catccacagt aagataagac cccccacccc accccgtcct gtcgttcccc 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 1320

1261 ctcctaaccc ttctgttatc gtccgtacga cccctacgcc acccgagata ccgaagactc 1320

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 1380

1321 cgcctttctt ggtcgacccc gagatccccc ataggggtgc gcgggacatc gccgcgtaat 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 1440

1381 tcgcgccgcc cacaccacca atgcgcgtcg cactggcgat gtgaacggtc gcgggatcgc 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 1500

1441 gggcgaggaa agcgaaagaa gggaaggaaa gagcggtgca agcggccgaa aggggcagtt 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 1560

1501 cgagatttag cccccgaggg aaatcccaag gctaaatcac gaaatgccgt ggagctgggg 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 1620

1561 ttttttgaac taatcccact accaagtgca tcacccggta gcgggactat ctgccaaaaa 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 1680

1621 gcgggaaact gcaacctcag gtgcaagaaa ttatcacctg agaacaaggt ttgaccttgt 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 1740

1681 tgtgagttgg gatagagcca gataagaaaa ctaaatattc cctaaaacgg ctaaagccgg 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 1800

1741 ataaccaatt ttttactcga ctaaattgtt tttaaattgc gcttaattaa gacaccttac 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 1860

1801 acacagtcaa tcccacacct ttcaggggtc cgaggggtcg tccgtcttca tacgtttcgt 1860

pBABE_3_pri ........ SV40_enh SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 1920

1861 acgtagagtt aatcagtcgt tggtccacac ctttcagggg tccgaggggt cgtccgtctt 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 1980

1921 catacgtttc gtacgtagag ttaatcagtc gttggtatca gggcggggat tgaggcgggt 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 2040

1981 agggcgggga ttgaggcggg tcaaggcggg taagaggcgg ggtaccgact gattaaaaaa 2040

..... SV40_enh SV40_pro SV40_origin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 2100

2041 aataaatacg tctccggctc cggcggagac ggagactcga taaggtcttc atcactcctc 2100

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

.......... StuI(2113) .......... SmaI(2137) .......... ..........

2101 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 2160

2101 cgaaaaaacc tccggatccg aaaacgtttt tcgagggccc tcgaacatat aggtaaaagc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 2220

2161 ctagactagt tctctgtcct actcctagca aagcgtacta acttgttcta cctaacgtgc 2220

.......... .......... .......... ........ ORF 3 Neomycin ........

.......... .......... .......... .......... .......... ..........

2221 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 2280

2221 gtccaagagg ccggcgaacc cacctctccg ataagccgat actgacccgt gttgtctgtt 2280

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 2340

2281 agccgacgag actacggcgg cacaaggccg acagtcgcgt ccccgcgggc caagaaaaac 2340

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt 2400

2341 agttctggct ggacaggcca cgggacttac ttgacgtcct gctccgtcgc gccgatagca 2400

.......... ........ ORF 3 Neomycin ........ .......... ..........

MscI(2406) .......... .......... .......... .......... ..........

2401 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 2460

2401 ccgaccggtg ctgcccgcaa ggaacgcgtc gacacgagct gcaacagtga cttcgccctt 2460

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 2520

2461 ccctgaccga cgataacccg cttcacggcc ccgtcctaga ggacagtaga gtggaacgag 2520

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 2580

2521 gacggctctt tcataggtag taccgactac gttacgccgc cgacgtatgc gaactaggcc 2580

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 2640

2581 gatggacggg taagctggtg gttcgctttg tagcgtagct cgctcgtgca tgagcctacc 2640

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 2700

2641 ttcggccaga acagctagtc ctactagacc tgcttctcgt agtccccgag cgcggtcggc 2700

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg 2760

2701 ttgacaagcg gtccgagttc cgcgcgtacg ggctgccgct cctagagcag cactgggtac 2760

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 2820

2761 cgctacggac gaacggctta tagtaccacc ttttaccggc gaaaagacct aagtagctga 2820

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 2880

2821 caccggccga cccacaccgc ctggcgatag tcctgtatcg caaccgatgg gcactataac 2880

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 2940

2881 gacttctcga accgccgctt acccgactgg cgaaggagca cgaaatgcca tagcggcgag 2940

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct 3000

2941 ggctaagcgt cgcgtagcgg aagatagcgg aagaactgct caagaagact cgccctgaga 3000

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc 3060

3001 ccccaagcgc tttactggct ggttcgctgc gggttggacg gtagtgctct aaagctaagg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 3120

3061 tggcggcgga agatactttc caacccgaag ccttagcaaa aggccctgcg gccgacctac 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca 3180

3121 taggaggtcg cgcccctaga gtacgacctc aagaagcggg tggggttgaa caaataacgt 3180

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

3181 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 3240

3181 cgaatattac caatgtttat ttcgttatcg tagtgtttaa agtgtttatt tcgtaaaaaa 3240

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata 3300

3241 agtgacgtaa gatcaacacc aaacaggttt gagtagttac atagaatagt acagacatat 3300

.......... ... SV40_PA_term EBV_rev_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 3360

3301 ggcagctgga gatcgatctc gaaccgcatt agtaccagta tcgacaaagg acacacttta 3360

.......... .......... .......... .... M13_reverse_pri M13_pUC_rev_pri

.......... .......... .......... .......... .......... ..........

3361 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 3420

3361 acaataggcg agtgttaagg tgtgttgtat gctcggcctt cgtatttcac atttcggacc 3420

M13_pUC_rev_pri .... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 3480

3421 ccacggatta ctcactcgat tgagtgtaat taacgcaacg cgagtgacgg gcgaaaggtc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 3540

3481 agccctttgg acagcacggt cgacgtaatt acttagccgg ttgcgcgccc ctctccgcca 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 3600

3541 aacgcataac ccgcgagaag gcgaaggagc gagtgactga gcgacgcgag ccagcaagcc 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 3660

3601 gacgccgctc gccatagtcg agtgagtttc cgccattatg ccaataggtg tcttagtccc 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 3720

3661 ctattgcgtc ctttcttgta cactcgtttt ccggtcgttt tccggtcctt ggcatttttc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 3780

3721 cggcgcaacg accgcaaaaa ggtatccgag gcggggggac tgctcgtagt gtttttagct 3780

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 3840

3781 gcgagttcag tctccaccgc tttgggctgt cctgatattt ctatggtccg caaaggggga 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 3900

3841 ccttcgaggg agcacgcgag aggacaaggc tgggacggcg aatggcctat ggacaggcgg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 3960

3901 aaagagggaa gcccttcgca ccgcgaaaga gtatcgagtg cgacatccat agagtcaagc 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 4020

3961 cacatccagc aagcgaggtt cgacccgaca cacgtgcttg gggggcaagt cgggctggcg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 4080

4021 acgcggaata ggccattgat agcagaactc aggttgggcc attctgtgct gaatagcggt 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 4140

4081 gaccgtcgtc ggtgaccatt gtcctaatcg tctcgctcca tacatccgcc acgatgtctc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 4200

4141 aagaacttca ccaccggatt gatgccgatg tgatcttctt gtcataaacc atagacgcga 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 4260

4201 gacgacttcg gtcaatggaa gcctttttct caaccatcga gaactaggcc gtttgtttgg 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 4320

4261 tggcgaccat cgccaccaaa aaaacaaacg ttcgtcgtct aatgcgcgtc tttttttcct 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 4380

4321 agagttcttc taggaaacta gaaaagatgc cccagactgc gagtcacctt gcttttgagt 4380

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 4440

4381 gcaattccct aaaaccagta ctctaatagt ttttcctaga agtggatcta ggaaaattta 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 4500

4441 atttttactt caaaatttag ttagatttca tatatactca tttgaaccag actgtcaatg 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 4560

4501 gttacgaatt agtcactccg tggatagagt cgctagacag ataaagcaag taggtatcaa 4560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 4620

4561 cggactgagg ggcagcacat ctattgatgc tatgccctcc cgaatggtag accggggtca 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 4680

4621 cgacgttact atggcgctct gggtgcgagt ggccgaggtc taaatagtcg ttatttggtc 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 4740

4681 ggtcggcctt cccggctcgc gtcttcacca ggacgttgaa ataggcggag gtaggtcaga 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 4800

4741 taattaacaa cggcccttcg atctcattca tcaagcggtc aattatcaaa cgcgttgcaa 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 4860

4801 caacggtaac gatgtccgta gcaccacagt gcgagcagca aaccataccg aagtaagtcg 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 4920

4861 aggccaaggg ttgctagttc cgctcaatgt actagggggt acaacacgtt ttttcgccaa 4920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 4980

4921 tcgaggaagc caggaggcta gcaacagtct tcattcaacc ggcgtcacaa tagtgagtac 4980

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 5040

4981 caataccgtc gtgacgtatt aagagaatga cagtacggta ggcattctac gaaaagacac 5040

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 5100

5041 tgaccactca tgagttggtt cagtaagact cttatcacat acgccgctgg ctcaacgaga 5100

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 5160

5101 acgggccgca gttatgccct attatggcgc ggtgtatcgt cttgaaattt tcacgagtag 5160

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 5220

5161 taaccttttg caagaagccc cgcttttgag agttcctaga atggcgacaa ctctaggtca 5220

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 5280

5221 agctacattg ggtgagcacg tgggttgact agaagtcgta gaaaatgaaa gtggtcgcaa 5280

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 5340

5281 agacccactc gtttttgtcc ttccgtttta cggcgttttt tcccttattc ccgctgtgcc 5340

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 5400

5341 tttacaactt atgagtatga gaaggaaaaa gttataataa cttcgtaaat agtcccaata 5400

ORF 3 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 5460

5401 acagagtact cgcctatgta taaacttaca taaatctttt tatttgttta tccccaaggc 5460

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cgcacatttc cccgaaaagt gccacctgac gtc....... .......... .......... 5493

5461 gcgtgtaaag gggcttttca cggtggactg cag....... .......... .......... 5493

.......... .......... .......... .......... .......... ..........