Sequence of pcDNA3.1(+)_myc-His

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Sequence of pcDNA3.1(+)_myc-His_B

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Sequence of pcDNA3.1(+)/myc-His B : 5497 BP; 1273 A; 1429 C; 1401 G; 1394 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatca 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

HindIII(902) KpnI(912) BamHI(920) ........ EcoRI(943) ..........

901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca 960

901 attcgaacca tggctcgagc ctaggtgatc aggtcacacc accttaagac gtctataggt 960

.......... .......... .......... .......... .......... ..........

NotI(970) XhoI(976) XbaI(982) .......... .......... ..........

961 gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaacaaaaac tcatctcaga 1020

961 cgtgtcaccg ccggcgagct cagatctccc gggcgccaag cttgtttttg agtagagtct 1020

.......... .......... .......... .......... c_myc ..........

.......... AgeI(1040) .......... .......... .......... ..........

1021 agaggatctg aatatgcata ccggtcatca tcaccatcac cattgagttt aaacccgctg 1080

1021 tctcctagac ttatacgtat ggccagtagt agtggtagtg gtaactcaaa tttgggcgac 1080

c_myc .......... .......... 6xHis .......... ..........

.......... .......... .......... .......... .......... ..........

1081 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 1140

1081 tagtcggagc tgacacggaa gatcaacggt cggtagacaa caaacgggga gggggcacgg 1140

......... BGH_rev_pri ........ bGH_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

1141 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 1200

1141 aaggaactgg gaccttccac ggtgagggtg acaggaaagg attattttac tcctttaacg 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 1260

1201 tagcgtaaca gactcatcca cagtaagata agacccccca ccccaccccg tcctgtcgtt 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc 1320

1261 ccccctccta acccttctgt tatcgtccgt acgaccccta cgccacccga gataccgaag 1320

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc 1380

1321 actccgcctt tcttggtcga ccccgagatc ccccataggg gtgcgcggga catcgccgcg 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 1440

1381 taattcgcgc cgcccacacc accaatgcgc gtcgcactgg cgatgtgaac ggtcgcggga 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 1500

1441 tcgcgggcga ggaaagcgaa agaagggaag gaaagagcgg tgcaagcggc cgaaaggggc 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 1560

1501 agttcgagat ttagcccccg agggaaatcc caaggctaaa tcacgaaatg ccgtggagct 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 1620

1561 ggggtttttt gaactaatcc cactaccaag tgcatcaccc ggtagcggga ctatctgcca 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 1680

1621 aaaagcggga aactgcaacc tcaggtgcaa gaaattatca cctgagaaca aggtttgacc 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 1740

1681 ttgttgtgag ttgggataga gccagataag aaaactaaat attccctaaa acggctaaag 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg 1800

1741 ccggataacc aattttttac tcgactaaat tgtttttaaa ttgcgcttaa ttaagacacc 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 1860

1801 ttacacacag tcaatcccac acctttcagg ggtccgaggg gtcgtccgtc ttcatacgtt 1860

pBABE_3_pri ........ ...... SV40_enh SV40_pro ...... ..........

.......... .......... .......... .......... .......... ..........

1861 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 1920

1861 tcgtacgtag agttaatcag tcgttggtcc acacctttca ggggtccgag gggtcgtccg 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 1980

1921 tcttcatacg tttcgtacgt agagttaatc agtcgttggt atcagggcgg ggattgaggc 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 2040

1981 gggtagggcg gggattgagg cgggtcaagg cgggtaagag gcggggtacc gactgattaa 2040

......... SV40_enh SV40_pro SV40_origin ......... ..........

.......... .......... .......... .......... .......... ..........

2041 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga 2100

2041 aaaaaataaa tacgtctccg gctccggcgg agacggagac tcgataaggt cttcatcact 2100

.. SV40_origin SV40pro_F_pri SV40_pro .. .......... ..........

.......... StuI(2117) .......... .......... SmaI(2141) ..........

2101 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt 2160

2101 cctccgaaaa aacctccgga tccgaaaacg tttttcgagg gccctcgaac atataggtaa 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg 2220

2161 aagcctagac tagttctctg tcctactcct agcaaagcgt actaacttgt tctacctaac 2220

.......... .......... .......... .......... ORF 1 Neomycin

.......... .......... .......... .......... .......... ..........

2221 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 2280

2221 gtgcgtccaa gaggccggcg aacccacctc tccgataagc cgatactgac ccgtgttgtc 2280

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 2340

2281 tgttagccga cgagactacg gcggcacaag gccgacagtc gcgtccccgc gggccaagaa 2340

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta 2400

2341 aaacagttct ggctggacag gccacgggac ttacttgacg tcctgctccg tcgcgccgat 2400

.......... ........ ORF 1 Neomycin ........ .......... ..........

MscI(2410) .......... .......... .......... .......... ..........

2401 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 2460

2401 agcaccgacc ggtgctgccc gcaaggaacg cgtcgacacg agctgcaaca gtgacttcgc 2460

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 2520

2461 ccttccctga ccgacgataa cccgcttcac ggccccgtcc tagaggacag tagagtggaa 2520

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 2580

2521 cgaggacggc tctttcatag gtagtaccga ctacgttacg ccgccgacgt atgcgaacta 2580

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 2640

2581 ggccgatgga cgggtaagct ggtggttcgc tttgtagcgt agctcgctcg tgcatgagcc 2640

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 2700

2641 taccttcggc cagaacagct agtcctacta gacctgcttc tcgtagtccc cgagcgcggt 2700

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc 2760

2701 cggcttgaca agcggtccga gttccgcgcg tacgggctgc cgctcctaga gcagcactgg 2760

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 2820

2761 gtaccgctac ggacgaacgg cttatagtac caccttttac cggcgaaaag acctaagtag 2820

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 2880

2821 ctgacaccgg ccgacccaca ccgcctggcg atagtcctgt atcgcaaccg atgggcacta 2880

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 2940

2881 taacgacttc tcgaaccgcc gcttacccga ctggcgaagg agcacgaaat gccatagcgg 2940

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga 3000

2941 cgagggctaa gcgtcgcgta gcggaagata gcggaagaac tgctcaagaa gactcgccct 3000

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 3060

3001 gagaccccaa gcgctttact ggctggttcg ctgcgggttg gacggtagtg ctctaaagct 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 3120

3061 aaggtggcgg cggaagatac tttccaaccc gaagccttag caaaaggccc tgcggccgac 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat 3180

3121 ctactaggag gtcgcgcccc tagagtacga cctcaagaag cgggtggggt tgaacaaata 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 3240

3181 acgtcgaata ttaccaatgt ttatttcgtt atcgtagtgt ttaaagtgtt tatttcgtaa 3240

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 3300

3241 aaaaagtgac gtaagatcaa caccaaacag gtttgagtag ttacatagaa tagtacagac 3300

.......... ... SV40_PA_term EBV_rev_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 3360

3301 atatggcagc tggagatcga tctcgaaccg cattagtacc agtatcgaca aaggacacac 3360

.......... .......... .......... ....... M13_reverse_pri .......

.......... .......... .......... .......... .......... ..........

3361 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 3420

3361 tttaacaata ggcgagtgtt aaggtgtgtt gtatgctcgg ccttcgtatt tcacatttcg 3420

M13_pUC_rev_pri .... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

3421 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 3480

3421 gaccccacgg attactcact cgattgagtg taattaacgc aacgcgagtg acgggcgaaa 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 3540

3481 ggtcagccct ttggacagca cggtcgacgt aattacttag ccggttgcgc gcccctctcc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 3600

3541 gccaaacgca taacccgcga gaaggcgaag gagcgagtga ctgagcgacg cgagccagca 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 3660

3601 agccgacgcc gctcgccata gtcgagtgag tttccgccat tatgccaata ggtgtcttag 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 3720

3661 tcccctattg cgtcctttct tgtacactcg ttttccggtc gttttccggt ccttggcatt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 3780

3721 tttccggcgc aacgaccgca aaaaggtatc cgaggcgggg ggactgctcg tagtgttttt 3780

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 3840

3781 agctgcgagt tcagtctcca ccgctttggg ctgtcctgat atttctatgg tccgcaaagg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 3900

3841 gggaccttcg agggagcacg cgagaggaca aggctgggac ggcgaatggc ctatggacag 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 3960

3901 gcggaaagag ggaagccctt cgcaccgcga aagagtatcg agtgcgacat ccatagagtc 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 4020

3961 aagccacatc cagcaagcga ggttcgaccc gacacacgtg cttggggggc aagtcgggct 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 4080

4021 ggcgacgcgg aataggccat tgatagcaga actcaggttg ggccattctg tgctgaatag 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 4140

4081 cggtgaccgt cgtcggtgac cattgtccta atcgtctcgc tccatacatc cgccacgatg 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 4200

4141 tctcaagaac ttcaccaccg gattgatgcc gatgtgatct tcttgtcata aaccatagac 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 4260

4201 gcgagacgac ttcggtcaat ggaagccttt ttctcaacca tcgagaacta ggccgtttgt 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 4320

4261 ttggtggcga ccatcgccac caaaaaaaca aacgttcgtc gtctaatgcg cgtctttttt 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 4380

4321 tcctagagtt cttctaggaa actagaaaag atgccccaga ctgcgagtca ccttgctttt 4380

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 4440

4381 gagtgcaatt ccctaaaacc agtactctaa tagtttttcc tagaagtgga tctaggaaaa 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 4500

4441 tttaattttt acttcaaaat ttagttagat ttcatatata ctcatttgaa ccagactgtc 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 4560

4501 aatggttacg aattagtcac tccgtggata gagtcgctag acagataaag caagtaggta 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 4620

4561 tcaacggact gaggggcagc acatctattg atgctatgcc ctcccgaatg gtagaccggg 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 4680

4621 gtcacgacgt tactatggcg ctctgggtgc gagtggccga ggtctaaata gtcgttattt 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 4740

4681 ggtcggtcgg ccttcccggc tcgcgtcttc accaggacgt tgaaataggc ggaggtaggt 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 4800

4741 cagataatta acaacggccc ttcgatctca ttcatcaagc ggtcaattat caaacgcgtt 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 4860

4801 gcaacaacgg taacgatgtc cgtagcacca cagtgcgagc agcaaaccat accgaagtaa 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 4920

4861 gtcgaggcca agggttgcta gttccgctca atgtactagg gggtacaaca cgttttttcg 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 4980

4921 ccaatcgagg aagccaggag gctagcaaca gtcttcattc aaccggcgtc acaatagtga 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 5040

4981 gtaccaatac cgtcgtgacg tattaagaga atgacagtac ggtaggcatt ctacgaaaag 5040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 5100

5041 acactgacca ctcatgagtt ggttcagtaa gactcttatc acatacgccg ctggctcaac 5100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 5160

5101 gagaacgggc cgcagttatg ccctattatg gcgcggtgta tcgtcttgaa attttcacga 5160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 5220

5161 gtagtaacct tttgcaagaa gccccgcttt tgagagttcc tagaatggcg acaactctag 5220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 5280

5221 gtcaagctac attgggtgag cacgtgggtt gactagaagt cgtagaaaat gaaagtggtc 5280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 5340

5281 gcaaagaccc actcgttttt gtccttccgt tttacggcgt tttttccctt attcccgctg 5340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 5400

5341 tgcctttaca acttatgagt atgagaagga aaaagttata ataacttcgt aaatagtccc 5400

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 5460

5401 aataacagag tactcgccta tgtataaact tacataaatc tttttatttg tttatcccca 5460

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tccgcgcaca tttccccgaa aagtgccacc tgacgtc... .......... .......... 5497

5461 aggcgcgtgt aaaggggctt ttcacggtgg actgcag... .......... .......... 5497

.......... .......... .......... .......... .......... ..........