Sequence of pcDNA3.1(+)_myc-His

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Sequence of pcDNA3.1(+)_myc-His_C

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Sequence of pcDNA3.1(+)/myc-His C : 5489 BP; 1273 A; 1428 C; 1395 G; 1393 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatca 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

HindIII(902) KpnI(912) BamHI(920) ........ EcoRI(943) ..........

901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca 960

901 attcgaacca tggctcgagc ctaggtgatc aggtcacacc accttaagac gtctataggt 960

.......... .......... .......... .......... .......... ..........

NotI(970) XhoI(976) .......... .......... .......... ..........

961 gcacagtggc ggccgctcga ggtcacccat tcgaacaaaa actcatctca gaagaggatc 1020

961 cgtgtcaccg ccggcgagct ccagtgggta agcttgtttt tgagtagagt cttctcctag 1020

.......... .......... .......... .......... c_myc ..........

.......... AgeI(1032) .......... .......... .......... ..........

1021 tgaatatgca taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct 1080

1021 acttatacgt atggccagta gtagtggtag tggtaactca aatttgggcg actagtcgga 1080

.......... .......... 6xHis .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 1140

1081 gctgacacgg aagatcaacg gtcggtagac aacaaacggg gagggggcac ggaaggaact 1140

BGH_rev_pri ....... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 1200

1141 gggaccttcc acggtgaggg tgacaggaaa ggattatttt actcctttaa cgtagcgtaa 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 1260

1201 cagactcatc cacagtaaga taagaccccc caccccaccc cgtcctgtcg ttccccctcc 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 1320

1261 taacccttct gttatcgtcc gtacgacccc tacgccaccc gagataccga agactccgcc 1320

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 1380

1321 tttcttggtc gaccccgaga tcccccatag gggtgcgcgg gacatcgccg cgtaattcgc 1380

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

1381 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 1440

1381 gccgcccaca ccaccaatgc gcgtcgcact ggcgatgtga acggtcgcgg gatcgcgggc 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 1500

1441 gaggaaagcg aaagaaggga aggaaagagc ggtgcaagcg gccgaaaggg gcagttcgag 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 1560

1501 atttagcccc cgagggaaat cccaaggcta aatcacgaaa tgccgtggag ctggggtttt 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 1620

1561 ttgaactaat cccactacca agtgcatcac ccggtagcgg gactatctgc caaaaagcgg 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 1680

1621 gaaactgcaa cctcaggtgc aagaaattat cacctgagaa caaggtttga ccttgttgtg 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 1740

1681 agttgggata gagccagata agaaaactaa atattcccta aaacggctaa agccggataa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg 1800

1741 ccaatttttt actcgactaa attgttttta aattgcgctt aattaagaca ccttacacac 1800

.......... .......... .......... .......... ......... pBABE_3_pri

.......... .......... .......... .......... .......... ..........

1801 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 1860

1801 agtcaatccc acacctttca ggggtccgag gggtcgtccg tcttcatacg tttcgtacgt 1860

pBABE_3_pri ........ SV40_enh SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 1920

1861 agagttaatc agtcgttggt ccacaccttt caggggtccg aggggtcgtc cgtcttcata 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 1980

1921 cgtttcgtac gtagagttaa tcagtcgttg gtatcagggc ggggattgag gcgggtaggg 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 2040

1981 cggggattga ggcgggtcaa ggcgggtaag aggcggggta ccgactgatt aaaaaaaata 2040

..... SV40_enh SV40_pro SV40_origin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt 2100

2041 aatacgtctc cggctccggc ggagacggag actcgataag gtcttcatca ctcctccgaa 2100

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

StuI(2109) .......... .......... SmaI(2133) .......... ..........

2101 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc 2160

2101 aaaacctccg gatccgaaaa cgtttttcga gggccctcga acatataggt aaaagcctag 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 2220

2161 actagttctc tgtcctactc ctagcaaagc gtactaactt gttctaccta acgtgcgtcc 2220

.......... .......... .......... ........ ORF 2 Neomycin ........

.......... .......... .......... .......... .......... ..........

2221 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 2280

2221 aagaggccgg cgaacccacc tctccgataa gccgatactg acccgtgttg tctgttagcc 2280

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 2340

2281 gacgagacta cggcggcaca aggccgacag tcgcgtcccc gcgggccaag aaaaacagtt 2340

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct 2400

2341 ctggctggac aggccacggg acttacttga cgtcctgctc cgtcgcgccg atagcaccga 2400

.......... ........ ORF 2 Neomycin ........ .......... ..........

MscI(2402) .......... .......... .......... .......... ..........

2401 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 2460

2401 ccggtgctgc ccgcaaggaa cgcgtcgaca cgagctgcaa cagtgacttc gcccttccct 2460

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 2520

2461 gaccgacgat aacccgcttc acggccccgt cctagaggac agtagagtgg aacgaggacg 2520

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 2580

2521 gctctttcat aggtagtacc gactacgtta cgccgccgac gtatgcgaac taggccgatg 2580

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 2640

2581 gacgggtaag ctggtggttc gctttgtagc gtagctcgct cgtgcatgag cctaccttcg 2640

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 2700

2641 gccagaacag ctagtcctac tagacctgct tctcgtagtc cccgagcgcg gtcggcttga 2700

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 2760

2701 caagcggtcc gagttccgcg cgtacgggct gccgctccta gagcagcact gggtaccgct 2760

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 2820

2761 acggacgaac ggcttatagt accacctttt accggcgaaa agacctaagt agctgacacc 2820

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 2880

2821 ggccgaccca caccgcctgg cgatagtcct gtatcgcaac cgatgggcac tataacgact 2880

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 2940

2881 tctcgaaccg ccgcttaccc gactggcgaa ggagcacgaa atgccatagc ggcgagggct 2940

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 3000

2941 aagcgtcgcg tagcggaaga tagcggaaga actgctcaag aagactcgcc ctgagacccc 3000

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg 3060

3001 aagcgcttta ctggctggtt cgctgcgggt tggacggtag tgctctaaag ctaaggtggc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc 3120

3061 ggcggaagat actttccaac ccgaagcctt agcaaaaggc cctgcggccg acctactagg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt 3180

3121 aggtcgcgcc cctagagtac gacctcaaga agcgggtggg gttgaacaaa taacgtcgaa 3180

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

3181 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 3240

3181 tattaccaat gtttatttcg ttatcgtagt gtttaaagtg tttatttcgt aaaaaaagtg 3240

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt 3300

3241 acgtaagatc aacaccaaac aggtttgagt agttacatag aatagtacag acatatggca 3300

.......... ... SV40_PA_term EBV_rev_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 3360

3301 gctggagatc gatctcgaac cgcattagta ccagtatcga caaaggacac actttaacaa 3360

.......... .......... ....... M13_reverse_pri .... M13_pUC_rev_pri

.......... .......... .......... .......... .......... ..........

3361 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 3420

3361 taggcgagtg ttaaggtgtg ttgtatgctc ggccttcgta tttcacattt cggaccccac 3420

.......... .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 3480

3421 ggattactca ctcgattgag tgtaattaac gcaacgcgag tgacgggcga aaggtcagcc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 3540

3481 ctttggacag cacggtcgac gtaattactt agccggttgc gcgcccctct ccgccaaacg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 3600

3541 cataacccgc gagaaggcga aggagcgagt gactgagcga cgcgagccag caagccgacg 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 3660

3601 ccgctcgcca tagtcgagtg agtttccgcc attatgccaa taggtgtctt agtcccctat 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 3720

3661 tgcgtccttt cttgtacact cgttttccgg tcgttttccg gtccttggca tttttccggc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 3780

3721 gcaacgaccg caaaaaggta tccgaggcgg ggggactgct cgtagtgttt ttagctgcga 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 3840

3781 gttcagtctc caccgctttg ggctgtcctg atatttctat ggtccgcaaa gggggacctt 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 3900

3841 cgagggagca cgcgagagga caaggctggg acggcgaatg gcctatggac aggcggaaag 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 3960

3901 agggaagccc ttcgcaccgc gaaagagtat cgagtgcgac atccatagag tcaagccaca 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 4020

3961 tccagcaagc gaggttcgac ccgacacacg tgcttggggg gcaagtcggg ctggcgacgc 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 4080

4021 ggaataggcc attgatagca gaactcaggt tgggccattc tgtgctgaat agcggtgacc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 4140

4081 gtcgtcggtg accattgtcc taatcgtctc gctccataca tccgccacga tgtctcaaga 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 4200

4141 acttcaccac cggattgatg ccgatgtgat cttcttgtca taaaccatag acgcgagacg 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 4260

4201 acttcggtca atggaagcct ttttctcaac catcgagaac taggccgttt gtttggtggc 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 4320

4261 gaccatcgcc accaaaaaaa caaacgttcg tcgtctaatg cgcgtctttt tttcctagag 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 4380

4321 ttcttctagg aaactagaaa agatgcccca gactgcgagt caccttgctt ttgagtgcaa 4380

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 4440

4381 ttccctaaaa ccagtactct aatagttttt cctagaagtg gatctaggaa aatttaattt 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 4500

4441 ttacttcaaa atttagttag atttcatata tactcatttg aaccagactg tcaatggtta 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 4560

4501 cgaattagtc actccgtgga tagagtcgct agacagataa agcaagtagg tatcaacgga 4560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 4620

4561 ctgaggggca gcacatctat tgatgctatg ccctcccgaa tggtagaccg gggtcacgac 4620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 4680

4621 gttactatgg cgctctgggt gcgagtggcc gaggtctaaa tagtcgttat ttggtcggtc 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 4740

4681 ggccttcccg gctcgcgtct tcaccaggac gttgaaatag gcggaggtag gtcagataat 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 4800

4741 taacaacggc ccttcgatct cattcatcaa gcggtcaatt atcaaacgcg ttgcaacaac 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 4860

4801 ggtaacgatg tccgtagcac cacagtgcga gcagcaaacc ataccgaagt aagtcgaggc 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 4920

4861 caagggttgc tagttccgct caatgtacta gggggtacaa cacgtttttt cgccaatcga 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 4980

4921 ggaagccagg aggctagcaa cagtcttcat tcaaccggcg tcacaatagt gagtaccaat 4980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 5040

4981 accgtcgtga cgtattaaga gaatgacagt acggtaggca ttctacgaaa agacactgac 5040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 5100

5041 cactcatgag ttggttcagt aagactctta tcacatacgc cgctggctca acgagaacgg 5100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 5160

5101 gccgcagtta tgccctatta tggcgcggtg tatcgtcttg aaattttcac gagtagtaac 5160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 5220

5161 cttttgcaag aagccccgct tttgagagtt cctagaatgg cgacaactct aggtcaagct 5220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 5280

5221 acattgggtg agcacgtggg ttgactagaa gtcgtagaaa atgaaagtgg tcgcaaagac 5280

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 5340

5281 ccactcgttt ttgtccttcc gttttacggc gttttttccc ttattcccgc tgtgccttta 5340

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 5400

5341 caacttatga gtatgagaag gaaaaagtta taataacttc gtaaatagtc ccaataacag 5400

ORF 2 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 5460

5401 agtactcgcc tatgtataaa cttacataaa tctttttatt tgtttatccc caaggcgcgt 5460

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 catttccccg aaaagtgcca cctgacgtc. .......... .......... .......... 5489

5461 gtaaaggggc ttttcacggt ggactgcag. .......... .......... .......... 5489

.......... .......... .......... .......... .......... ..........