Sequence of pcDNA3.1(+)

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Sequence of pcDNA3.1(+)

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Sequence of pcDNA3.1(+) : 5428 BP; 1251 A; 1411 C; 1386 G; 1380 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... NruI(208) .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

AflII(908),HindIII(911) KpnI(921) BamHI(929) ...... ..........

901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc 960

901 caaatttgaa ttcgaaccat ggctcgagcc taggtgatca ggtcacacca ccttaagacg 960

.......... .......... .......... .......... .......... ..........

EcoRV64) NotI(979) XhoI(985) XbaI(991) ApaI(1001) ..........

961 agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca 1020

961 tctataggtc gtgtcaccgc cggcgagctc agatctcccg ggcaaatttg ggcgactagt 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 1080

1021 cggagctgac acggaagatc aacggtcggt agacaacaaa cggggagggg gcacggaagg 1080

BGH_rev_pri ........ ......... bGH_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

1081 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 1140

1081 aactgggacc ttccacggtg agggtgacag gaaaggatta ttttactcct ttaacgtagc 1140

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 1200

1141 gtaacagact catccacagt aagataagac cccccacccc accccgtcct gtcgttcccc 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 1260

1201 ctcctaaccc ttctgttatc gtccgtacga cccctacgcc acccgagata ccgaagactc 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 1320

1261 cgcctttctt ggtcgacccc gagatccccc ataggggtgc gcgggacatc gccgcgtaat 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 1380

1321 tcgcgccgcc cacaccacca atgcgcgtcg cactggcgat gtgaacggtc gcgggatcgc 1380

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 1440

1381 gggcgaggaa agcgaaagaa gggaaggaaa gagcggtgca agcggccgaa aggggcagtt 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 1500

1441 cgagatttag cccccgaggg aaatcccaag gctaaatcac gaaatgccgt ggagctgggg 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 1560

1501 ttttttgaac taatcccact accaagtgca tcacccggta gcgggactat ctgccaaaaa 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 1620

1561 gcgggaaact gcaacctcag gtgcaagaaa ttatcacctg agaacaaggt ttgaccttgt 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 1680

1621 tgtgagttgg gatagagcca gataagaaaa ctaaatattc cctaaaacgg ctaaagccgg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 1740

1681 ataaccaatt ttttactcga ctaaattgtt tttaaattgc gcttaattaa gacaccttac 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 1800

1741 acacagtcaa tcccacacct ttcaggggtc cgaggggtcg tccgtcttca tacgtttcgt 1800

pBABE_3_pri ........ SV40_enh SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 1860

1801 acgtagagtt aatcagtcgt tggtccacac ctttcagggg tccgaggggt cgtccgtctt 1860

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 1920

1861 catacgtttc gtacgtagag ttaatcagtc gttggtatca gggcggggat tgaggcgggt 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 1980

1921 agggcgggga ttgaggcggg tcaaggcggg taagaggcgg ggtaccgact gattaaaaaa 1980

..... SV40_enh SV40_pro SV40_origin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 2040

1981 aataaatacg tctccggctc cggcggagac ggagactcga taaggtcttc atcactcctc 2040

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

.......... StuI(2053) .......... SmaI(2077) .......... ..........

2041 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 2100

2041 cgaaaaaacc tccggatccg aaaacgtttt tcgagggccc tcgaacatat aggtaaaagc 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 2160

2101 ctagactagt tctctgtcct actcctagca aagcgtacta acttgttcta cctaacgtgc 2160

.......... .......... .......... ........ ORF 3 Neomycin ........

.......... .......... .......... .......... .......... ..........

2161 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 2220

2161 gtccaagagg ccggcgaacc cacctctccg ataagccgat actgacccgt gttgtctgtt 2220

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 2280

2221 agccgacgag actacggcgg cacaaggccg acagtcgcgt ccccgcgggc caagaaaaac 2280

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt 2340

2281 agttctggct ggacaggcca cgggacttac ttgacgtcct gctccgtcgc gccgatagca 2340

.......... ........ ORF 3 Neomycin ........ .......... ..........

MscI(2346) .......... .......... .......... .......... ..........

2341 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 2400

2341 ccgaccggtg ctgcccgcaa ggaacgcgtc gacacgagct gcaacagtga cttcgccctt 2400

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 2460

2401 ccctgaccga cgataacccg cttcacggcc ccgtcctaga ggacagtaga gtggaacgag 2460

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 2520

2461 gacggctctt tcataggtag taccgactac gttacgccgc cgacgtatgc gaactaggcc 2520

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 2580

2521 gatggacggg taagctggtg gttcgctttg tagcgtagct cgctcgtgca tgagcctacc 2580

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 2640

2581 ttcggccaga acagctagtc ctactagacc tgcttctcgt agtccccgag cgcggtcggc 2640

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg 2700

2641 ttgacaagcg gtccgagttc cgcgcgtacg ggctgccgct cctagagcag cactgggtac 2700

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 2760

2701 cgctacggac gaacggctta tagtaccacc ttttaccggc gaaaagacct aagtagctga 2760

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 2820

2761 caccggccga cccacaccgc ctggcgatag tcctgtatcg caaccgatgg gcactataac 2820

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 2880

2821 gacttctcga accgccgctt acccgactgg cgaaggagca cgaaatgcca tagcggcgag 2880

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct 2940

2881 ggctaagcgt cgcgtagcgg aagatagcgg aagaactgct caagaagact cgccctgaga 2940

.......... ........ ORF 3 Neomycin ........ .......... ..........

BstBI(2946) ........ .......... .......... .......... ..........

2941 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac 3000

2941 ccccaagctt tactggctgg ttcgctgcgg gttggacggt agtgctctaa agctaaggtg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 3060

3001 gcggcggaag atactttcca acccgaagcc ttagcaaaag gccctgcggc cgacctacta 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc 3120

3061 ggaggtcgcg cccctagagt acgacctcaa gaagcgggtg gggttgaaca aataacgtcg 3120

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

3121 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 3180

3121 aatattacca atgtttattt cgttatcgta gtgtttaaag tgtttatttc gtaaaaaaag 3180

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc 3240

3181 tgacgtaaga tcaacaccaa acaggtttga gtagttacat agaatagtac agacatatgg 3240

.......... ... SV40_PA_term EBV_rev_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 3300

3241 cagctggaga tcgatctcga accgcattag taccagtatc gacaaaggac acactttaac 3300

.......... .......... ....... M13_reverse_pri .... M13_pUC_rev_pri

.......... .......... .......... .......... .......... ..........

3301 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 3360

3301 aataggcgag tgttaaggtg tgttgtatgc tcggccttcg tatttcacat ttcggacccc 3360

M13_pUC_rev_pri .... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 3420

3361 acggattact cactcgattg agtgtaatta acgcaacgcg agtgacgggc gaaaggtcag 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 3480

3421 ccctttggac agcacggtcg acgtaattac ttagccggtt gcgcgcccct ctccgccaaa 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 3540

3481 cgcataaccc gcgagaaggc gaaggagcga gtgactgagc gacgcgagcc agcaagccga 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 3600

3541 cgccgctcgc catagtcgag tgagtttccg ccattatgcc aataggtgtc ttagtcccct 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 3660

3601 attgcgtcct ttcttgtaca ctcgttttcc ggtcgttttc cggtccttgg catttttccg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 3720

3661 gcgcaacgac cgcaaaaagg tatccgaggc ggggggactg ctcgtagtgt ttttagctgc 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 3780

3721 gagttcagtc tccaccgctt tgggctgtcc tgatatttct atggtccgca aagggggacc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 3840

3781 ttcgagggag cacgcgagag gacaaggctg ggacggcgaa tggcctatgg acaggcggaa 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 3900

3841 agagggaagc ccttcgcacc gcgaaagagt atcgagtgcg acatccatag agtcaagcca 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 3960

3901 catccagcaa gcgaggttcg acccgacaca cgtgcttggg gggcaagtcg ggctggcgac 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 4020

3961 gcggaatagg ccattgatag cagaactcag gttgggccat tctgtgctga atagcggtga 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 4080

4021 ccgtcgtcgg tgaccattgt cctaatcgtc tcgctccata catccgccac gatgtctcaa 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 4140

4081 gaacttcacc accggattga tgccgatgtg atcttcttgt cataaaccat agacgcgaga 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 4200

4141 cgacttcggt caatggaagc ctttttctca accatcgaga actaggccgt ttgtttggtg 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 4260

4201 gcgaccatcg ccaaaaaaac aaacgttcgt cgtctaatgc gcgtcttttt ttcctagagt 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 4320

4261 tcttctagga aactagaaaa gatgccccag actgcgagtc accttgcttt tgagtgcaat 4320

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 4380

4321 tccctaaaac cagtactcta atagtttttc ctagaagtgg atctaggaaa atttaatttt 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 4440

4381 tacttcaaaa tttagttaga tttcatatat actcatttga accagactgt caatggttac 4440

.......... .......... .......... .......... ....... ORF 1 Ampicillin

.......... .......... .......... .......... .......... ..........

4441 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 4500

4441 gaattagtca ctccgtggat agagtcgcta gacagataaa gcaagtaggt atcaacggac 4500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 4560

4501 tgaggggcag cacatctatt gatgctatgc cctcccgaat ggtagaccgg ggtcacgacg 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 4620

4561 ttactatggc gctctgggtg cgagtggccg aggtctaaat agtcgttatt tggtcggtcg 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 4680

4621 gccttcccgg ctcgcgtctt caccaggacg ttgaaatagg cggaggtagg tcagataatt 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 4740

4681 aacaacggcc cttcgatctc attcatcaag cggtcaatta tcaaacgcgt tgcaacaacg 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 4800

4741 gtaacgatgt ccgtagcacc acagtgcgag cagcaaacca taccgaagta agtcgaggcc 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 4860

4801 aagggttgct agttccgctc aatgtactag ggggtacaac acgttttttc gccaatcgag 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 4920

4861 gaagccagga ggctagcaac agtcttcatt caaccggcgt cacaatagtg agtaccaata 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 4980

4921 ccgtcgtgac gtattaagag aatgacagta cggtaggcat tctacgaaaa gacactgacc 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 5040

4981 actcatgagt tggttcagta agactcttat cacatacgcc gctggctcaa cgagaacggg 5040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 5100

5041 ccgcagttat gccctattat ggcgcggtgt atcgtcttga aattttcacg agtagtaacc 5100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 5160

5101 ttttgcaaga agccccgctt ttgagagttc ctagaatggc gacaactcta ggtcaagcta 5160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 5220

5161 cattgggtga gcacgtgggt tgactagaag tcgtagaaaa tgaaagtggt cgcaaagacc 5220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 5280

5221 cactcgtttt tgtccttccg ttttacggcg ttttttccct tattcccgct gtgcctttac 5280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 5340

5281 aacttatgag tatgagaagg aaaaagttat aataacttcg taaatagtcc caataacaga 5340

ORF 1 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 5400

5341 gtactcgcct atgtataaac ttacataaat ctttttattt gtttatcccc aaggcgcgtg 5400

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 atttccccga aaagtgccac ctgacgtc.. .......... .......... .......... 5428

5401 taaaggggct tttcacggtg gactgcag.. .......... .......... .......... 5428

.......... .......... .......... .......... .......... ..........