Sequence of pcDNA3.1(-)

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pcDNA3.1(-)

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pcDNA3.1(-) : 5427 BP; 1253 A; 1414 C; 1382 G; 1378 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... NruI(208) .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

ApaI913),XbaI(915) XhoI(921) NotI(927) EcoRV(946) ..........

901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc 960

901 caaatttgcc cgggagatct gagctcgccg gcggtgacac gacctataga cgtcttaagg 960

.......... .......... .......... .......... .......... ..........

........ BamHI(977) KpnI(993),HindIII(995),AflII(998) ........

961 accacactgg actagtggat ccgagctcgg taccaagctt aagtttaaac cgctgatcag 1020

961 tggtgtgacc tgatcaccta ggctcgagcc atggttcgaa ttcaaatttg gcgactagtc 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 1080

1021 ggagctgaca cggaagatca acggtcggta gacaacaaac ggggaggggg cacggaagga 1080

BGH_rev_pri ........ ......... bGH_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 1140

1081 actgggacct tccacggtga gggtgacagg aaaggattat tttactcctt taacgtagcg 1140

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 1200

1141 taacagactc atccacagta agataagacc ccccacccca ccccgtcctg tcgttccccc 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg 1260

1201 tcctaaccct tctgttatcg tccgtacgac ccctacgcca cccgagatac cgaagactcc 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa 1320

1261 gcctttcttg gtcgaccccg agatccccca taggggtgcg cgggacatcg ccgcgtaatt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 1380

1321 cgcgccgccc acaccaccaa tgcgcgtcgc actggcgatg tgaacggtcg cgggatcgcg 1380

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 1440

1381 ggcgaggaaa gcgaaagaag ggaaggaaag agcggtgcaa gcggccgaaa ggggcagttc 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 1500

1441 gagatttagc ccccgaggga aatcccaagg ctaaatcacg aaatgccgtg gagctggggt 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 1560

1501 tttttgaact aatcccacta ccaagtgcat cacccggtag cgggactatc tgccaaaaag 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 1620

1561 cgggaaactg caacctcagg tgcaagaaat tatcacctga gaacaaggtt tgaccttgtt 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 1680

1621 gtgagttggg atagagccag ataagaaaac taaatattcc ctaaaacggc taaagccgga 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt 1740

1681 taaccaattt tttactcgac taaattgttt ttaaattgcg cttaattaag acaccttaca 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 1800

1741 cacagtcaat cccacacctt tcaggggtcc gaggggtcgt ccgtcttcat acgtttcgta 1800

pBABE_3_pri ........ SV40_enh SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 1860

1801 cgtagagtta atcagtcgtt ggtccacacc tttcaggggt ccgaggggtc gtccgtcttc 1860

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 1920

1861 atacgtttcg tacgtagagt taatcagtcg ttggtatcag ggcggggatt gaggcgggta 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 1980

1921 gggcggggat tgaggcgggt caaggcgggt aagaggcggg gtaccgactg attaaaaaaa 1980

..... SV40_enh SV40_pro SV40_origin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg 2040

1981 ataaatacgt ctccggctcc ggcggagacg gagactcgat aaggtcttca tcactcctcc 2040

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

.......... StuI(2052) .......... SmaI(2076) .......... ..........

2041 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg 2100

2041 gaaaaaacct ccggatccga aaacgttttt cgagggccct cgaacatata ggtaaaagcc 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 2160

2101 tagactagtt ctctgtccta ctcctagcaa agcgtactaa cttgttctac ctaacgtgcg 2160

.......... .......... .......... ........ ORF 2 Neomycin ........

.......... .......... .......... .......... .......... ..........

2161 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 2220

2161 tccaagaggc cggcgaaccc acctctccga taagccgata ctgacccgtg ttgtctgtta 2220

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 2280

2221 gccgacgaga ctacggcggc acaaggccga cagtcgcgtc cccgcgggcc aagaaaaaca 2280

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg 2340

2281 gttctggctg gacaggccac gggacttact tgacgtcctg ctccgtcgcg ccgatagcac 2340

.......... ........ ORF 2 Neomycin ........ .......... ..........

MscI(2345) .......... .......... .......... .......... ..........

2341 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 2400

2341 cgaccggtgc tgcccgcaag gaacgcgtcg acacgagctg caacagtgac ttcgcccttc 2400

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 2460

2401 cctgaccgac gataacccgc ttcacggccc cgtcctagag gacagtagag tggaacgagg 2460

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 2520

2461 acggctcttt cataggtagt accgactacg ttacgccgcc gacgtatgcg aactaggccg 2520

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 2580

2521 atggacgggt aagctggtgg ttcgctttgt agcgtagctc gctcgtgcat gagcctacct 2580

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 2640

2581 tcggccagaa cagctagtcc tactagacct gcttctcgta gtccccgagc gcggtcggct 2640

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg 2700

2641 tgacaagcgg tccgagttcc gcgcgtacgg gctgccgctc ctagagcagc actgggtacc 2700

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 2760

2701 gctacggacg aacggcttat agtaccacct tttaccggcg aaaagaccta agtagctgac 2760

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 2820

2761 accggccgac ccacaccgcc tggcgatagt cctgtatcgc aaccgatggg cactataacg 2820

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 2880

2821 acttctcgaa ccgccgctta cccgactggc gaaggagcac gaaatgccat agcggcgagg 2880

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 2940

2881 gctaagcgtc gcgtagcgga agatagcgga agaactgctc aagaagactc gccctgagac 2940

.......... ........ ORF 2 Neomycin ........ .......... ..........

BstBI(2945) ........ .......... .......... .......... ..........

2941 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc 3000

2941 cccaagcttt actggctggt tcgctgcggg ttggacggta gtgctctaaa gctaaggtgg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 3060

3001 cggcggaaga tactttccaa cccgaagcct tagcaaaagg ccctgcggcc gacctactag 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct 3120

3061 gaggtcgcgc ccctagagta cgacctcaag aagcgggtgg ggttgaacaa ataacgtcga 3120

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

3121 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 3180

3121 atattaccaa tgtttatttc gttatcgtag tgtttaaagt gtttatttcg taaaaaaagt 3180

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg 3240

3181 gacgtaagat caacaccaaa caggtttgag tagttacata gaatagtaca gacatatggc 3240

.......... ... SV40_PA_term EBV_rev_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 3300

3241 agctggagat cgatctcgaa ccgcattagt accagtatcg acaaaggaca cactttaaca 3300

.......... .......... ....... M13_reverse_pri .... M13_pUC_rev_pri

.......... .......... .......... .......... .......... ..........

3301 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 3360

3301 ataggcgagt gttaaggtgt gttgtatgct cggccttcgt atttcacatt tcggacccca 3360

.......... .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 3420

3361 cggattactc actcgattga gtgtaattaa cgcaacgcga gtgacgggcg aaaggtcagc 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 3480

3421 cctttggaca gcacggtcga cgtaattact tagccggttg cgcgcccctc tccgccaaac 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 3540

3481 gcataacccg cgagaaggcg aaggagcgag tgactgagcg acgcgagcca gcaagccgac 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 3600

3541 gccgctcgcc atagtcgagt gagtttccgc cattatgcca ataggtgtct tagtccccta 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 3660

3601 ttgcgtcctt tcttgtacac tcgttttccg gtcgttttcc ggtccttggc atttttccgg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 3720

3661 cgcaacgacc gcaaaaaggt atccgaggcg gggggactgc tcgtagtgtt tttagctgcg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 3780

3721 agttcagtct ccaccgcttt gggctgtcct gatatttcta tggtccgcaa agggggacct 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 3840

3781 tcgagggagc acgcgagagg acaaggctgg gacggcgaat ggcctatgga caggcggaaa 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 3900

3841 gagggaagcc cttcgcaccg cgaaagagta tcgagtgcga catccataga gtcaagccac 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 3960

3901 atccagcaag cgaggttcga cccgacacac gtgcttgggg ggcaagtcgg gctggcgacg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 4020

3961 cggaataggc cattgatagc agaactcagg ttgggccatt ctgtgctgaa tagcggtgac 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 4080

4021 cgtcgtcggt gaccattgtc ctaatcgtct cgctccatac atccgccacg atgtctcaag 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 4140

4081 aacttcacca ccggattgat gccgatgtga tcttcttgtc ataaaccata gacgcgagac 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 4200

4141 gacttcggtc aatggaagcc tttttctcaa ccatcgagaa ctaggccgtt tgtttggtgg 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 4260

4201 cgaccatcgc caaaaaaaca aacgttcgtc gtctaatgcg cgtctttttt tcctagagtt 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 4320

4261 cttctaggaa actagaaaag atgccccaga ctgcgagtca ccttgctttt gagtgcaatt 4320

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 4380

4321 ccctaaaacc agtactctaa tagtttttcc tagaagtgga tctaggaaaa tttaattttt 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 4440

4381 acttcaaaat ttagttagat ttcatatata ctcatttgaa ccagactgtc aatggttacg 4440

.......... .......... .......... .......... ....... ORF 3 Ampicillin

.......... .......... .......... .......... .......... ..........

4441 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 4500

4441 aattagtcac tccgtggata gagtcgctag acagataaag caagtaggta tcaacggact 4500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 4560

4501 gaggggcagc acatctattg atgctatgcc ctcccgaatg gtagaccggg gtcacgacgt 4560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 4620

4561 tactatggcg ctctgggtgc gagtggccga ggtctaaata gtcgttattt ggtcggtcgg 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 4680

4621 ccttcccggc tcgcgtcttc accaggacgt tgaaataggc ggaggtaggt cagataatta 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 4740

4681 acaacggccc ttcgatctca ttcatcaagc ggtcaattat caaacgcgtt gcaacaacgg 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 4800

4741 taacgatgtc cgtagcacca cagtgcgagc agcaaaccat accgaagtaa gtcgaggcca 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 4860

4801 agggttgcta gttccgctca atgtactagg gggtacaaca cgttttttcg ccaatcgagg 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 4920

4861 aagccaggag gctagcaaca gtcttcattc aaccggcgtc acaatagtga gtaccaatac 4920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 4980

4921 cgtcgtgacg tattaagaga atgacagtac ggtaggcatt ctacgaaaag acactgacca 4980

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 5040

4981 ctcatgagtt ggttcagtaa gactcttatc acatacgccg ctggctcaac gagaacgggc 5040

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 5100

5041 cgcagttatg ccctattatg gcgcggtgta tcgtcttgaa attttcacga gtagtaacct 5100

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 5160

5101 tttgcaagaa gccccgcttt tgagagttcc tagaatggcg acaactctag gtcaagctac 5160

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 5220

5161 attgggtgag cacgtgggtt gactagaagt cgtagaaaat gaaagtggtc gcaaagaccc 5220

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 5280

5221 actcgttttt gtccttccgt tttacggcgt tttttccctt attcccgctg tgcctttaca 5280

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 5340

5281 acttatgagt atgagaagga aaaagttata ataacttcgt aaatagtccc aataacagag 5340

ORF 3 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 5400

5341 tactcgccta tgtataaact tacataaatc tttttatttg tttatcccca aggcgcgtgt 5400

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tttccccgaa aagtgccacc tgacgtc... .......... .......... .......... 5427

5401 aaaggggctt ttcacggtgg actgcag... .......... .......... .......... 5427

.......... .......... .......... .......... .......... ..........