Sequence of pcDNA3.1D

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Sequence of pcDNA3.1D_V5-His-TOPO

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Sequence of pcDNA3.1D V5-His-TOPO : 5514 BP; 1270 A; 1443 C; 1403 G; 1398 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccagctgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

SpeI(249) .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctg ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatca 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

HindIII(902) KpnI(912) ........ .......... .......... ..........

901 taagcttggt accgagctcg gatccagtac ccttcaccaa gggtcaagac aattctgcag 960

901 attcgaacca tggctcgagc ctaggtcatg ggaagtggtt cccagttctg ttaagacgtc 960

.......... .......... .......... .......... .......... ..........

EcoRV NotI XhoI XbaI ApaI,SacII(1002),BstBI(1006)

961 atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta 1020

961 tataggtcgt gtcaccgccg gcgagctcag atctcccggg cgccaagctt ccattcggat 1020

.......... .......... .......... .......... .......... V5

.......... .......... .......... AgeI(1056) .......... ..........

1021 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt 1080

1021 agggattggg agaggagcca gagctaagat gcgcatggcc agtagtagtg gtagtggtaa 1080

.......... V5 .......... .......... 6xHis ..........

.......... .......... .......... .......... .......... ..........

1081 gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt 1140

1081 ctcaaatttg ggcgactagt cggagctgac acggaagatc aacggtcggt agacaacaaa 1140

.......... ......... BGH_rev_pri ........ bGH_PA_term .........

.......... .......... .......... .......... .......... ..........

1141 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 1200

1141 cggggagggg gcacggaagg aactgggacc ttccacggtg agggtgacag gaaaggatta 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 1260

1201 ttttactcct ttaacgtagc gtaacagact catccacagt aagataagac cccccacccc 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 1320

1261 accccgtcct gtcgttcccc ctcctaaccc ttctgttatc gtccgtacga cccctacgcc 1320

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg 1380

1321 acccgagata ccgaagactc cgcctttctt ggtcgacccc gagatccccc ataggggtgc 1380

bGH_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 1440

1381 gcgggacatc gccgcgtaat tcgcgccgcc cacaccacca atgcgcgtcg cactggcgat 1440

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 1500

1441 gtgaacggtc gcgggatcgc gggcgaggaa agcgaaagaa gggaaggaaa gagcggtgca 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg 1560

1501 agcggccgaa aggggcagtt cgagatttag ccccgtaggg aaatcccaag gctaaatcac 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 1620

1561 gaaatgccgt ggagctgggg ttttttgaac taatcccact accaagtgca tcacccggta 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 1680

1621 gcgggactat ctgccaaaaa gcgggaaact gcaacctcag gtgcaagaaa ttatcacctg 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 1740

1681 agaacaaggt ttgaccttgt tgtgagttgg gatagagcca gataagaaaa ctaaatattc 1740

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 1800

1741 cctaaaaccc ctaaagccgg ataaccaatt ttttactcga ctaaattgtt tttaaattgc 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg 1860

1801 gcttaattaa gacaccttac acacagtcaa tcccacacct ttcaggggtc cgaggggtcc 1860

.......... ......... pBABE_3_pri SV40_enh SV40_pro .........

.......... .......... .......... .......... .......... ..........

1861 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 1920

1861 gtccgtcttc atacgtttcg tacgtagagt taatcagtcg ttggtccaca cctttcaggg 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 1980

1921 gtccgagggg tcgtccgtct tcatacgttt cgtacgtaga gttaatcagt cgttggtatc 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 2040

1981 agggcgggga ttgaggcggg tagggcgggg attgaggcgg gtcaaggcgg gtaagaggcg 2040

.......... ..... SV40_enh SV40_pro SV40_origin ..... ..........

.......... .......... .......... .......... .......... ..........

2041 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 2100

2041 gggtaccgac tgattaaaaa aaataaatac gtctccggct ccggcggaga cggagactcg 2100

....... SV40_origin SV40_pro SV40pro_F_pri ....... ..........

.......... .......... .......... StuI(2134) .......... SmaI(2158)

2101 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 2160

2101 ataaggtctt catcactcct ccgaaaaaac ctccggatcc gaaaacgttt ttcgagggcc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga 2220

2161 ctcgaacata taggtaaaag cctagactag ttctctgtcc tactcctagc aaagcgtact 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 2280

2221 aacttgttct acctaacgtg cgtccaagag gccggcgaac ccacctctcc gataagccga 2280

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 2340

2281 tactgacccg tgttgtctgt tagccgacga gactacggcg gcacaaggcc gacagtcgcg 2340

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg 2400

2341 tccccgcggg ccaagaaaaa cagttctggc tggacaggcc acgggactta cttgacgtcc 2400

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... MscI(2427) .......... .......... ..........

2401 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 2460

2401 tgctccgtcg cgccgatagc accgaccggt gctgcccgca aggaacgcgt cgacacgagc 2460

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 2520

2461 tgcaacagtg acttcgccct tccctgaccg acgataaccc gcttcacggc cccgtcctag 2520

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 2580

2521 aggacagtag agtggaacga ggacggctct ttcataggta gtaccgacta cgttacgccg 2580

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 2640

2581 ccgacgtatg cgaactaggc cgatggacgg gtaagctggt ggttcgcttt gtagcgtagc 2640

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 2700

2641 tcgctcgtgc atgagcctac cttcggccag aacagctagt cctactagac ctgcttctcg 2700

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg 2760

2701 tagtccccga gcgcggtcgg cttgacaagc ggtccgagtt ccgcgcgtac gggctgccgc 2760

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 2820

2761 tcctagagca gcactgggta ccgctacgga cgaacggctt atagtaccac cttttaccgg 2820

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 2880

2821 cgaaaagacc taagtagctg acaccggccg acccacaccg cctggcgata gtcctgtatc 2880

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 2940

2881 gcaaccgatg ggcactataa cgacttctcg aaccgccgct tacccgactg gcgaaggagc 2940

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 3000

2941 acgaaatgcc atagcggcga gggctaagcg tcgcgtagcg gaagatagcg gaagaactgc 3000

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 agttcttctg agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg 3060

3001 tcaagaagac tcgccctgag accccaagcg ctttactggc tggttcgctg cgggttggac 3060

ORF 3 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 3120

3061 ggtagtgctc taaagctaag gtggcggcgg aagatacttt ccaacccgaa gccttagcaa 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 3180

3121 aaggccctgc ggccgaccta ctaggaggtc gcgcccctag agtacgacct caagaagcgg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 3240

3181 gtggggttga acaaataacg tcgaatatta ccaatgttta tttcgttatc gtagtgttta 3240

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa a ctcatcaat 3300

3241 aagtgtttat ttcgtaaaaa aagtgacgta agatcaacac caaacaggtt tgagtagtta 3300

.......... ......... SV40_PA_term ........ EBV_rev_pri .........

.......... .......... .......... .......... .......... ..........

3301 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca 3360

3301 catagaatag tacagacata tggcagctgg agatcgatct cgaaccgcat tagtaccagt 3360

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 3420

3361 atcgacaaag gacacacttt aacaataggc gagtgttaag gtgtgttgta tgctcggcct 3420

M13_reverse_pri . M13_pUC_rev_pri ....... .......... lac_pro

.......... .......... .......... .......... .......... ..........

3421 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 3480

3421 tcgtatttca catttcggac cccacggatt actcactcga ttgagtgtaa ttaacgcaac 3480

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 3540

3481 gcgagtgacg ggcgaaaggt cagccctttg gacagcacgg tcgacgtaat tacttagccg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 3600

3541 gttgcgcgcc cctctccgcc aaacgcataa cccgcgagaa ggcgaaggag cgagtgactg 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 3660

3601 agcgacgcga gccagcaagc cgacgccgct cgccatagtc gagtgagttt ccgccattat 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 3720

3661 gccaataggt gtcttagtcc cctattgcgt cctttcttgt acactcgttt tccggtcgtt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 3780

3721 ttccggtcct tggcattttt ccggcgcaac gaccgcaaaa aggtatccga ggcgggggga 3780

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3781 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 3840

3781 ctgctcgtag tgtttttagc tgcgagttca gtctccaccg ctttgggctg tcctgatatt 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 3900

3841 tctatggtcc gcaaaggggg accttcgagg gagcacgcga gaggacaagg ctgggacggc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca 3960

3901 gaatggccta tggacaggcg gaaagaggga agcccttcgc accgcgaaag agttacgagt 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 4020

3961 gcgacatcca tagagtcaag ccacatccag caagcgaggt tcgacccgac acacgtgctt 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 4080

4021 ggggggcaag tcgggctggc gacgcggaat aggccattga tagcagaact caggttgggc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 4140

4081 cattctgtgc tgaatagcgg tgaccgtcgt cggtgaccat tgtcctaatc gtctcgctcc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 4200

4141 atacatccgc cacgatgtct caagaacttc accaccggat tgatgccgat gtgatcttcc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 4260

4201 tgtcataaac catagacgcg agacgacttc ggtcaatgga agcctttttc tcaaccatcg 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 4320

4261 agaactaggc cgtttgtttg gtggcgacca tcgccaccaa aaaaacaaac gttcgtcgtc 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 4380

4321 taatgcgcgt ctttttttcc tagagttctt ctaggaaact agaaaagatg ccccagactg 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 4440

4381 cgagtcacct tgcttttgag tgcaattccc taaaaccagt actctaatag tttttcctag 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 4500

4441 aagtggatct aggaaaattt aatttttact tcaaaattta gttagatttc atatatactc 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 4560

4501 atttgaacca gactgtcaat ggttacgaat tagtcactcc gtggatagag tcgctagaca 4560

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4561 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 4620

4561 gataaagcaa gtaggtatca acggactgag gggcagcaca tctattgatg ctatgccctc 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 4680

4621 ccgaatggta gaccggggtc acgacgttac tatggcgctc tgggtgcgag tggccgaggt 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 4740

4681 ctaaatagtc gttatttggt cggtcggcct tcccggctcg cgtcttcacc aggacgttga 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 4800

4741 aataggcgga ggtaggtcag ataattaaca acggcccttc gatctcattc atcaagcggt 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 4860

4801 caattatcaa acgcgttgca acaacggtaa cgatgtccgt agcaccacag tgcgagcagc 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 4920

4861 aaaccatacc gaagtaagtc gaggccaagg gttgctagtt ccgctcaatg tactaggggg 4920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 4980

4921 tacaacacgt tttttcgcca atcgaggaag ccaggaggct agcaacagtc ttcattcaac 4980

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 5040

4981 cggcgtcaca atagtgagta ccaataccgt cgtgacgtat taagagaatg acagtacggt 5040

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 5100

5041 aggcattcta cgaaaagaca ctgaccactc atgagttggt tcagtaagac tcttatcaca 5100

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 5160

5101 tacgccgctg gctcaacgag aacgggccgc agttatgccc tattatggcg cggtgtatcg 5160

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 5220

5161 tcttgaaatt ttcacgagta gtaacctttt gcaagaagcc ccgcttttga gagttcctag 5220

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 5280

5221 aatggcgaca actctaggtc aagctacatt gggtgagcac gtgggttgac tagaagtcgt 5280

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 5340

5281 agaaaatgaa agtggtcgca aagacccact cgtttttgtc cttccgtttt acggcgtttt 5340

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 5400

5341 ttcccttatt cccgctgtgc ctttacaact tatgagtatg agaaggaaaa agttataata 5400

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 5460

5401 acttcgtaaa tagtcccaat aacagagtac tcgcctatgt ataaacttac ataaatcttt 5460

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc...... 5514

5461 ttatttgttt atccccaagg cgcgtgtaaa ggggcttttc acggtggact gcag...... 5514

.......... .......... .......... .......... .......... ..........