Sequence of pcDNA3.1_CT-GFP

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Sequence of pcDNA3.1_CT-GFP_Fusion

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Sequence of pcDNA3.1/CT-GFP Fusion : 6141 BP; 1477 A; 1559 C; 1532 G; 1573 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccagctgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctg ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatca 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

.......... KpnI(912) .......... .......... EcoRI(943) ..........

901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca 960

901 attcgaacca tggctcgagc ctaggtgatc aggtcacacc accttaagac gtctataggt 960

.......... .......... .......... .......... .......... ..........

NotI(970) .......... XbaI(982) NheI(991) .......... ..........

961 gcacagtggc ggccgctcga gtctagaatg gctagcaaag gagaagaact tttcactgga 1020

961 cgtgtcaccg ccggcgagct cagatcttac cgatcgtttc ctcttcttga aaagtgacct 1020

.......... .......... .......... ........ ORF 1 GFP_ORF ........

.......... .......... .......... .......... .......... ..........

1021 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt 1080

1021 caacagggtt aagaacaact taatctacca ctacaattac ccgtgtttaa aagacagtca 1080

........ GFP_R_pri ORF 1 GFP_ORF ........ ....... GFPfusionREV_pri

.......... .......... .......... .......... .......... ..........

1081 ggagagggtg aaggtgatgc tacatacgga aagcttaccc ttaaatttat ttgcactact 1140

1081 cctctcccac ttccactacg atgtatgcct ttcgaatggg aatttaaata aacgtgatga 1140

.......... ........ ORF 1 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ggaaaactac ctgttccatg gccaacactt gtcactactt tctcttatgg tgttcaatgc 1200

1141 ccttttgatg gacaaggtac cggttgtgaa cagtgatgaa agagaatacc acaagttacg 1200

.......... ........ ORF 1 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ttttcccgtt atccggatca tatgaaacgg catgactttt tcaagagtgc catgcccgaa 1260

1201 aaaagggcaa taggcctagt atactttgcc gtactgaaaa agttctcacg gtacgggctt 1260

.......... ........ ORF 1 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ggttatgtac aggaacgcac tatatctttc aaagatgacg ggaactacaa gacgcgtgct 1320

1261 ccaatacatg tccttgcgtg atatagaaag tttctactgc ccttgatgtt ctgcgcacga 1320

.......... ........ ORF 1 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg agttaaaagg tattgatttt 1380

1321 cttcagttca aacttccact atgggaacaa ttagcatagc tcaattttcc ataactaaaa 1380

.......... ........ ORF 1 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aaagaagatg gaaacattct cggacacaaa ctcgagtaca actataactc acacaatgta 1440

1381 tttcttctac ctttgtaaga gcctgtgttt gagctcatgt tgatattgag tgtgttacat 1440

.......... ........ ORF 1 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat tcgccacaac 1500

1441 atgtagtgcc gtctgtttgt tttcttacct tagtttcgat tgaagtttta agcggtgttg 1500

.......... ........ ORF 1 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 attgaagatg gatccgttca actagcagac cattatcaac aaaatactcc aattggcgat 1560

1501 taacttctac ctaggcaagt tgatcgtctg gtaatagttg ttttatgagg ttaaccgcta 1560

.......... ........ ORF 1 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... ......... BstBI(1612)

1561 ggccctgtcc ttttaccaga caaccattac ctgtcgacac aatctgccct ttcgaaagat 1620

1561 ccgggacagg aaaatggtct gttggtaatg gacagctgtg ttagacggga aagctttcta 1620

.......... ........ ORF 1 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc tgggattaca 1680

1621 gggttgcttt tcgcactggt gtaccaggaa gaactcaaac attgacgacg accctaatgt 1680

.......... ........ ORF 1 GFP_ORF GFP_F_pri ........ ..........

.......... .......... .......... .......... .......... ..........

1681 catggcatgg atgagctcta caaataatga attaaacccg ctgatcagcc tcgactgtgc 1740

1681 gtaccgtacc tactcgagat gtttattact taatttgggc gactagtcgg agctgacacg 1740

........ ORF 1 GFP_ORF ........ .......... ... BGH_rev_pri bGH_PA_term

.......... .......... .......... .......... .......... ..........

1741 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 1800

1741 gaagatcaac ggtcggtaga caacaaacgg ggagggggca cggaaggaac tgggaccttc 1800

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 1860

1801 cacggtgagg gtgacaggaa aggattattt tactccttta acgtagcgta acagactcat 1860

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 1920

1861 ccacagtaag ataagacccc ccaccccacc ccgtcctgtc gttccccctc ctaacccttc 1920

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca 1980

1921 tgttatcgtc cgtacgaccc ctacgccacc cgagataccg aagactccgc ctttcttggt 1980

......... bGH_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg 2040

1981 cgaccccgag atcccccata ggggtgcgcg ggacatcgcc gcgtaattcg cgccgcccac 2040

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

2041 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 2100

2041 accaccaatg cgcgtcgcac tggcgatgtg aacggtcgcg ggatcgcggg cgaggaaagc 2100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 2160

2101 gaaagaaggg aaggaaagag cggtgcaagc ggccgaaagg ggcagttcga gatttagccc 2160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 2220

2161 cgtagggaaa tcccaaggct aaatcacgaa atgccgtgga gctggggttt tttgaactaa 2220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 2280

2221 tcccactacc aagtgcatca cccggtagcg ggactatctg ccaaaaagcg ggaaactgca 2280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 2340

2281 acctcaggtg caagaaatta tcacctgaga acaaggtttg accttgttgt gagttgggat 2340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa 2400

2341 agagccagat aagaaaacta aatattccct aaaaccccta aagccggata accaattttt 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg 2460

2401 tactcgacta aattgttttt aaattgcgct taattaagac accttacaca cagtcaatcc 2460

.......... .......... .......... .......... ..... pBABE_3_pri SV40_enh

.......... .......... .......... .......... .......... ..........

2461 gtgtggaaag tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt 2520

2461 cacacctttc aggggtccga ggggtccgtc cgtcttcata cgtttcgtac gtagagttaa 2520

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 2580

2521 tcagtcgttg gtccacacct ttcaggggtc cgaggggtcg tccgtcttca tacgtttcgt 2580

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 2640

2581 acgtagagtt aatcagtcgt tggtatcagg gcggggattg aggcgggtag ggcggggatt 2640

.......... ...... SV40_enh SV40_pro ...... ......... SV40_origin

.......... .......... .......... .......... .......... ..........

2641 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 2700

2641 gaggcgggtc aaggcgggta agaggcgggg taccgactga ttaaaaaaaa taaatacgtc 2700

..... SV40_enh SV40_pro SV40_origin ..... ........ SV40pro_F_pri

.......... .......... .......... .......... .......... ..........

2701 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 2760

2701 tccggctccg gcggagacgg agactcgata aggtcttcat cactcctccg aaaaaacctc 2760

SV40pro_F_pri SV40_pro ...... .......... .......... ..........

StuI(2761) .......... SmaI(2785) .......... .......... ..........

2761 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag 2820

2761 cggatccgaa aacgtttttc gagggccctc gaacatatag gtaaaagcct agactagttc 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 2880

2821 tctgtcctac tcctagcaaa gcgtactaac ttgttctacc taacgtgcgt ccaagaggcc 2880

.......... .......... .......... ........ ORF 3 Neomycin ........

.......... .......... .......... .......... .......... ..........

2881 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 2940

2881 ggcgaaccca cctctccgat aagccgatac tgacccgtgt tgtctgttag ccgacgagac 2940

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... NarI(2972) .......... ..........

2941 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 3000

2941 tacggcggca caaggccgac agtcgcgtcc ccgcgggcca agaaaaacag ttctggctgg 3000

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga 3060

3001 acaggccacg ggacttactt gacgtcctgc tccgtcgcgc cgatagcacc gaccggtgct 3060

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 3120

3061 gcccgcaagg aacgcgtcga cacgagctgc aacagtgact tcgcccttcc ctgaccgacg 3120

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 3180

3121 ataacccgct tcacggcccc gtcctagagg acagtagagt ggaacgagga cggctctttc 3180

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 3240

3181 ataggtagta ccgactacgt tacgccgccg acgtatgcga actaggccga tggacgggta 3240

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 3300

3241 agctggtggt tcgctttgta gcgtagctcg ctcgtgcatg agcctacctt cggccagaac 3300

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 3360

3301 agctagtcct actagacctg cttctcgtag tccccgagcg cggtcggctt gacaagcggt 3360

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 3420

3361 ccgagttccg cgcgtacggg ctgccgctcc tagagcagca ctgggtaccg ctacggacga 3420

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 3480

3421 acggcttata gtaccacctt ttaccggcga aaagacctaa gtagctgaca ccggccgacc 3480

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 3540

3481 cacaccgcct ggcgatagtc ctgtatcgca accgatgggc actataacga cttctcgaac 3540

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 3600

3541 cgccgcttac ccgactggcg aaggagcacg aaatgccata gcggcgaggg ctaagcgtcg 3600

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgcgaa 3660

3601 cgtagcggaa gatagcggaa gaactgctca agaagactcg ccctgagacc ccaagcgctt 3660

........ ORF 3 Neomycin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc 3720

3661 tactggctgg ttcgctgcgg gttggacggt agtgctctaa agctaaggtg gcggcggaag 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 3780

3721 atactttcca acccgaagcc ttagcaaaag gccctgcggc cgacctacta ggaggtcgcg 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt 3840

3781 cccctagagt acgacctcaa gaagcgggtg gggttgaaca aataacgtcg aatattacca 3840

.......... .......... .......... ......... SV40_PA_term .........

.......... .......... .......... .......... .......... ..........

3841 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 3900

3841 atgtttattt cgttatcgta gtgtttaaag tgtttatttc gtaaaaaaag tgacgtaaga 3900

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct 3960

3901 tcaacaccaa acaggtttga gtagttacat agaatagtac agacatatgg cagctggaga 3960

... SV40_PA_term EBV_rev_pri ... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 4020

3961 tcgatctcga accgcattag taccagtatc gacaaaggac acactttaac aataggcgag 4020

.......... ....... M13_reverse_pri .... M13_pUC_rev_pri .......

.......... .......... .......... .......... .......... ..........

4021 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 4080

4021 tgttaaggtg tgttgtatgc tcggccttcg tatttcacat ttcggacccc acggattact 4080

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 4140

4081 cactcgattg agtgtaatta acgcaacgcg agtgacgggc gaaaggtcag ccctttggac 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 4200

4141 agcacggtcg acgtaattac ttagccggtt gcgcgcccct ctccgccaaa cgcataaccc 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 4260

4201 gcgagaaggc gaaggagcga gtgactgagc gacgcgagcc agcaagccga cgccgctcgc 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 4320

4261 catagtcgag tgagtttccg ccattatgcc aataggtgtc ttagtcccct attgcgtcct 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 4380

4321 ttcttgtaca ctcgttttcc ggtcgttttc cggtccttgg catttttccg gcgcaacgac 4380

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

4381 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 4440

4381 cgcaaaaagg tatccgaggc ggggggactg ctcgtagtgt ttttagctgc gagttcagtc 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 4500

4441 tccaccgctt tgggctgtcc tgatatttct atggtccgca aagggggacc ttcgagggag 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 4560

4501 cacgcgagag gacaaggctg ggacggcgaa tggcctatgg acaggcggaa agagggaagc 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 4620

4561 ccttcgcacc gcgaaagagt tacgagtgcg acatccatag agtcaagcca catccagcaa 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 4680

4621 gcgaggttcg acccgacaca cgtgcttggg gggcaagtcg ggctggcgac gcggaatagg 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 4740

4681 ccattgatag cagaactcag gttgggccat tctgtgctga atagcggtga ccgtcgtcgg 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 4800

4741 tgaccattgt cctaatcgtc tcgctccata catccgccac gatgtctcaa gaacttcacc 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 4860

4801 accggattga tgccgatgtg atcttcctgt cataaaccat agacgcgaga cgacttcggt 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 4920

4861 caatggaagc ctttttctca accatcgaga actaggccgt ttgtttggtg gcgaccatcg 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 4980

4921 ccaccaaaaa aacaaacgtt cgtcgtctaa tgcgcgtctt tttttcctag agttcttcta 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 5040

4981 ggaaactaga aaagatgccc cagactgcga gtcaccttgc ttttgagtgc aattccctaa 5040

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 5100

5041 aaccagtact ctaatagttt ttcctagaag tggatctagg aaaatttaat ttttacttca 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 5160

5101 aaatttagtt agatttcata tatactcatt tgaaccagac tgtcaatggt tacgaattag 5160

.......... .......... .......... .......... ....... ORF 3 Ampicillin

.......... .......... .......... .......... .......... ..........

5161 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 5220

5161 tcactccgtg gatagagtcg ctagacagat aaagcaagta ggtatcaacg gactgagggg 5220

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 5280

5221 cagcacatct attgatgcta tgccctcccg aatggtagac cggggtcacg acgttactat 5280

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 5340

5281 ggcgctctgg gtgcgagtgg ccgaggtcta aatagtcgtt atttggtcgg tcggccttcc 5340

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 5400

5341 cggctcgcgt cttcaccagg acgttgaaat aggcggaggt aggtcagata attaacaacg 5400

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 5460

5401 gcccttcgat ctcattcatc aagcggtcaa ttatcaaacg cgttgcaaca acggtaacga 5460

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 5520

5461 tgtccgtagc accacagtgc gagcagcaaa ccataccgaa gtaagtcgag gccaagggtt 5520

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 5580

5521 gctagttccg ctcaatgtac tagggggtac aacacgtttt ttcgccaatc gaggaagcca 5580

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 5640

5581 ggaggctagc aacagtcttc attcaaccgg cgtcacaata gtgagtacca ataccgtcgt 5640

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 5700

5641 gacgtattaa gagaatgaca gtacggtagg cattctacga aaagacactg accactcatg 5700

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 5760

5701 agttggttca gtaagactct tatcacatac gccgctggct caacgagaac gggccgcagt 5760

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 5820

5761 tatgccctat tatggcgcgg tgtatcgtct tgaaattttc acgagtagta accttttgca 5820

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 5880

5821 agaagccccg cttttgagag ttcctagaat ggcgacaact ctaggtcaag ctacattggg 5880

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 5940

5881 tgagcacgtg ggttgactag aagtcgtaga aaatgaaagt ggtcgcaaag acccactcgt 5940

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 6000

5941 ttttgtcctt ccgttttacg gcgttttttc ccttattccc gctgtgcctt tacaacttat 6000

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 6060

6001 gagtatgaga aggaaaaagt tataataact tcgtaaatag tcccaataac agagtactcg 6060

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

6061 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 6120

6061 cctatgtata aacttacata aatcttttta tttgtttatc cccaaggcgc gtgtaaaggg 6120

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 cgaaaagtgc cacctgacgt c......... .......... .......... .......... 6141

6121 gcttttcacg gtggactgca g......... .......... .......... .......... 6141

.......... .......... .......... .......... .......... ..........