Sequence of pcDNA3.1_His

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Sequence of pcDNA3.1_His_B

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Sequence of pcDNA3.1/His B : 5515 BP; 1275 A; 1425 C; 1413 G; 1402 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

SpeI(249) .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

CAG_enh ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

... AflII(908),HindIII(911) ... .......... .......... ..........

901 gtttaaactt aagcttacca tggggggttc tcatcatcat catcatcatg gtatggctag 960

901 caaatttgaa ttcgaatggt accccccaag agtagtagta gtagtagtac cataccgatc 960

.......... .......... .......... 6xHis ........ Xpress_fwd_pri

.......... .......... .......... .......... .......... ..........

961 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggtacctaa 1020

961 gtactgacca cctgtcgttt acccagccct agacatgctg ctactgctat tccatggatt 1020

Xpress_fwd_pri T7_gene10_leader T7_leader Xpress_EK ..........

BamHI EcoRI036) EcoRV(1048) NotI(1063) XhoI(1069) ..

1021 ggatccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga 1080

1021 cctaggtcac accaccttaa gacgtctata ggtcgtgtca ccgccggcga gctcagatct 1080

.......... .......... .......... .......... .......... ..........

ApaI(1085) .......... .......... .......... .......... ..........

1081 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt 1140

1081 cccgggcaaa tttgggcgac tagtcggagc tgacacggaa gatcaacggt cggtagacaa 1140

.......... .......... ........ BGH_rev_pri bGH_PA_term ........

.......... .......... .......... .......... .......... ..........

1141 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 1200

1141 caaacgggga gggggcacgg aaggaactgg gaccttccac ggtgagggtg acaggaaagg 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 1260

1201 attattttac tcctttaacg tagcgtaaca gactcatcca cagtaagata agacccccca 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 1320

1261 ccccaccccg tcctgtcgtt ccccctccta acccttctgt tatcgtccgt acgaccccta 1320

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc 1380

1321 cgccacccga gataccgaag actccgcctt tcttggtcga ccccgagatc ccccataggg 1380

bGH_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 1440

1381 gtgcgcggga catcgccgcg taattcgcgc cgcccacacc accaatgcgc gtcgcactgg 1440

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 1500

1441 cgatgtgaac ggtcgcggga tcgcgggcga ggaaagcgaa agaagggaag gaaagagcgg 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 1560

1501 tgcaagcggc cgaaaggggc agttcgagat ttagcccccg agggaaatcc caaggctaaa 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 1620

1561 tcacgaaatg ccgtggagct ggggtttttt gaactaatcc cactaccaag tgcatcaccc 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 1680

1621 ggtagcggga ctatctgcca aaaagcggga aactgcaacc tcaggtgcaa gaaattatca 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 1740

1681 cctgagaaca aggtttgacc ttgttgtgag ttgggataga gccagataag aaaactaaat 1740

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 1800

1741 attccctaaa acggctaaag ccggataacc aattttttac tcgactaaat tgtttttaaa 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 1860

1801 ttgcgcttaa ttaagacacc ttacacacag tcaatcccac acctttcagg ggtccgaggg 1860

.......... ......... pBABE_3_pri ..... SV40_enh SV40_pro ......

.......... .......... .......... .......... .......... ..........

1861 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 1920

1861 gtcgtccgtc ttcatacgtt tcgtacgtag agttaatcag tcgttggtcc acacctttca 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 1980

1921 ggggtccgag gggtcgtccg tcttcatacg tttcgtacgt agagttaatc agtcgttggt 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 2040

1981 atcagggcgg ggattgaggc gggtagggcg gggattgagg cgggtcaagg cgggtaagag 2040

.......... ...... SV40_enh SV40_pro ..... SV40_origin .........

.......... .......... .......... .......... .......... ..........

2041 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 2100

2041 gcggggtacc gactgattaa aaaaaataaa tacgtctccg gctccggcgg agacggagac 2100

.......... ... SV40_pro SV40_origin SV40pro_F_pri ... ..........

.......... .......... .......... .......... .......... ..........

2101 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 2160

2101 tcgataaggt cttcatcact cctccgaaaa aacctccgga tccgaaaacg tttttcgagg 2160

.......... .......... .......... .......... .......... ..........

SmaI(2161) .......... .......... .......... .......... ..........

2161 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca 2220

2161 gccctcgaac atataggtaa aagcctagac tagttctctg tcctactcct agcaaagcgt 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 2280

2221 actaacttgt tctacctaac gtgcgtccaa gaggccggcg aacccacctc tccgataagc 2280

.......... .......... ORF 3 Neomycin .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 2340

2281 cgatactgac ccgtgttgtc tgttagccga cgagactacg gcggcacaag gccgacagtc 2340

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 2400

2341 gcgtccccgc gggccaagaa aaacagttct ggctggacag gccacgggac ttacttgacg 2400

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... MscI(2430) .......... .......... ..........

2401 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 2460

2401 tcctgctccg tcgcgccgat agcaccgacc ggtgctgccc gcaaggaacg cgtcgacacg 2460

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 2520

2461 agctgcaaca gtgacttcgc ccttccctga ccgacgataa cccgcttcac ggccccgtcc 2520

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 2580

2521 tagaggacag tagagtggaa cgaggacggc tctttcatag gtagtaccga ctacgttacg 2580

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 2640

2581 ccgccgacgt atgcgaacta ggccgatgga cgggtaagct ggtggttcgc tttgtagcgt 2640

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 2700

2641 agctcgctcg tgcatgagcc taccttcggc cagaacagct agtcctacta gacctgcttc 2700

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg 2760

2701 tcgtagtccc cgagcgcggt cggcttgaca agcggtccga gttccgcgcg tacgggctgc 2760

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 2820

2761 cgctcctaga gcagcactgg gtaccgctac ggacgaacgg cttatagtac caccttttac 2820

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 2880

2821 cggcgaaaag acctaagtag ctgacaccgg ccgacccaca ccgcctggcg atagtcctgt 2880

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 2940

2881 atcgcaaccg atgggcacta taacgacttc tcgaaccgcc gcttacccga ctggcgaagg 2940

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 3000

2941 agcacgaaat gccatagcgg cgagggctaa gcgtcgcgta gcggaagata gcggaagaac 3000

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... ......... BstBI(3030) ......... .......... ..........

3001 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct 3060

3001 tgctcaagaa gactcgccct gagaccccaa gctttactgg ctggttcgct gcgggttgga 3060

ORF 3 Neomycin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 3120

3061 cggtagtgct ctaaagctaa ggtggcggcg gaagatactt tccaacccga agccttagca 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 3180

3121 aaaggccctg cggccgacct actaggaggt cgcgccccta gagtacgacc tcaagaagcg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 3240

3181 ggtggggttg aacaaataac gtcgaatatt accaatgttt atttcgttat cgtagtgttt 3240

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

3241 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca a actcatcaa 3300

3241 aaagtgttta tttcgtaaaa aaagtgacgt aagatcaaca ccaaacaggt ttgagtagtt 3300

.......... ......... SV40_PA_term ........ EBV_rev_pri .........

.......... .......... .......... .......... .......... ..........

3301 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc 3360

3301 acatagaata gtacagacat atggcagctg gagatcgatc tcgaaccgca ttagtaccag 3360

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 3420

3361 tatcgacaaa ggacacactt taacaatagg cgagtgttaa ggtgtgttgt atgctcggcc 3420

M13_reverse_pri . M13_pUC_rev_pri ....... .......... lac_pro

.......... .......... .......... .......... .......... ..........

3421 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 3480

3421 ttcgtatttc acatttcgga ccccacggat tactcactcg attgagtgta attaacgcaa 3480

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 3540

3481 cgcgagtgac gggcgaaagg tcagcccttt ggacagcacg gtcgacgtaa ttacttagcc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 3600

3541 ggttgcgcgc ccctctccgc caaacgcata acccgcgaga aggcgaagga gcgagtgact 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 3660

3601 gagcgacgcg agccagcaag ccgacgccgc tcgccatagt cgagtgagtt tccgccatta 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 3720

3661 tgccaatagg tgtcttagtc ccctattgcg tcctttcttg tacactcgtt ttccggtcgt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 3780

3721 tttccggtcc ttggcatttt tccggcgcaa cgaccgcaaa aaggtatccg aggcgggggg 3780

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3781 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 3840

3781 actgctcgta gtgtttttag ctgcgagttc agtctccacc gctttgggct gtcctgatat 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 3900

3841 ttctatggtc cgcaaagggg gaccttcgag ggagcacgcg agaggacaag gctgggacgg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 3960

3901 cgaatggcct atggacaggc ggaaagaggg aagcccttcg caccgcgaaa gagtatcgag 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 4020

3961 tgcgacatcc atagagtcaa gccacatcca gcaagcgagg ttcgacccga cacacgtgct 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 4080

4021 tggggggcaa gtcgggctgg cgacgcggaa taggccattg atagcagaac tcaggttggg 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 4140

4081 ccattctgtg ctgaatagcg gtgaccgtcg tcggtgacca ttgtcctaat cgtctcgctc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 4200

4141 catacatccg ccacgatgtc tcaagaactt caccaccgga ttgatgccga tgtgatcttc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 4260

4201 ttgtcataaa ccatagacgc gagacgactt cggtcaatgg aagccttttt ctcaaccatc 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 4320

4261 gagaactagg ccgtttgttt ggtggcgacc atcgccacca aaaaaacaaa cgttcgtcgt 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 4380

4321 ctaatgcgcg tctttttttc ctagagttct tctaggaaac tagaaaagat gccccagact 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 4440

4381 gcgagtcacc ttgcttttga gtgcaattcc ctaaaaccag tactctaata gtttttccta 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 4500

4441 gaagtggatc taggaaaatt taatttttac ttcaaaattt agttagattt catatatact 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 4560

4501 catttgaacc agactgtcaa tggttacgaa ttagtcactc cgtggataga gtcgctagac 4560

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4561 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 4620

4561 agataaagca agtaggtatc aacggactga ggggcagcac atctattgat gctatgccct 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 4680

4621 cccgaatggt agaccggggt cacgacgtta ctatggcgct ctgggtgcga gtggccgagg 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 4740

4681 tctaaatagt cgttatttgg tcggtcggcc ttcccggctc gcgtcttcac caggacgttg 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 4800

4741 aaataggcgg aggtaggtca gataattaac aacggccctt cgatctcatt catcaagcgg 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 4860

4801 tcaattatca aacgcgttgc aacaacggta acgatgtccg tagcaccaca gtgcgagcag 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 4920

4861 caaaccatac cgaagtaagt cgaggccaag ggttgctagt tccgctcaat gtactagggg 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 4980

4921 gtacaacacg ttttttcgcc aatcgaggaa gccaggaggc tagcaacagt cttcattcaa 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 5040

4981 ccggcgtcac aatagtgagt accaataccg tcgtgacgta ttaagagaat gacagtacgg 5040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 5100

5041 taggcattct acgaaaagac actgaccact catgagttgg ttcagtaaga ctcttatcac 5100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 5160

5101 atacgccgct ggctcaacga gaacgggccg cagttatgcc ctattatggc gcggtgtatc 5160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 5220

5161 gtcttgaaat tttcacgagt agtaaccttt tgcaagaagc cccgcttttg agagttccta 5220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 5280

5221 gaatggcgac aactctaggt caagctacat tgggtgagca cgtgggttga ctagaagtcg 5280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 5340

5281 tagaaaatga aagtggtcgc aaagacccac tcgtttttgt ccttccgttt tacggcgttt 5340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 5400

5341 tttcccttat tcccgctgtg cctttacaac ttatgagtat gagaaggaaa aagttataat 5400

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 5460

5401 aacttcgtaa atagtcccaa taacagagta ctcgcctatg tataaactta cataaatctt 5460

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc..... 5515

5461 tttatttgtt tatccccaag gcgcgtgtaa aggggctttt cacggtggac tgcag..... 5515

.......... .......... .......... .......... .......... ..........