Sequence of pcDNA3.1_His

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Sequence of pcDNA3.1_His_C

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Sequence of pcDNA3.1/His C : 5513 BP; 1274 A; 1425 C; 1413 G; 1401 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

SpeI(249) .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

... AflII(908),HindIII(911) ... .......... .......... ..........

901 gtttaaactt aagcttacca tggggggttc tcatcatcat catcatcatg gtatggctag 960

901 caaatttgaa ttcgaatggt accccccaag agtagtagta gtagtagtac cataccgatc 960

.......... .......... .......... 6xHis ........ Xpress_fwd_pri

.......... .......... .......... .......... .......... ..........

961 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggtaccagg 1020

961 gtactgacca cctgtcgttt acccagccct agacatgctg ctactgctat tccatggtcc 1020

Xpress_fwd_pri T7_gene10_leader T7_leader Xpress_EK ..........

........ EcoRI(1034) EcoRV(1046) .. NotI(1061) XhoI(1067) ....

1021 atccagtgtg gtggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg 1080

1021 taggtcacac caccttaaga cgtctatagg tcgtgtcacc gccggcgagc tcagatctcc 1080

.......... .......... .......... .......... .......... ..........

ApaI(1083) .......... .......... .......... .......... ..........

1081 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 1140

1081 cgggcaaatt tgggcgacta gtcggagctg acacggaaga tcaacggtcg gtagacaaca 1140

.......... .......... ........ BGH_rev_pri bGH_PA_term ........

.......... .......... .......... .......... .......... ..........

1141 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 1200

1141 aacggggagg gggcacggaa ggaactggga ccttccacgg tgagggtgac aggaaaggat 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 1260

1201 tattttactc ctttaacgta gcgtaacaga ctcatccaca gtaagataag accccccacc 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 1320

1261 ccaccccgtc ctgtcgttcc ccctcctaac ccttctgtta tcgtccgtac gacccctacg 1320

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca 1380

1321 ccacccgaga taccgaagac tccgcctttc ttggtcgacc ccgagatccc ccataggggt 1380

bGH_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 1440

1381 gcgcgggaca tcgccgcgta attcgcgccg cccacaccac caatgcgcgt cgcactggcg 1440

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 1500

1441 atgtgaacgg tcgcgggatc gcgggcgagg aaagcgaaag aagggaagga aagagcggtg 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 1560

1501 caagcggccg aaaggggcag ttcgagattt agcccccgag ggaaatccca aggctaaatc 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 1620

1561 acgaaatgcc gtggagctgg ggttttttga actaatccca ctaccaagtg catcacccgg 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 1680

1621 tagcgggact atctgccaaa aagcgggaaa ctgcaacctc aggtgcaaga aattatcacc 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 1740

1681 tgagaacaag gtttgacctt gttgtgagtt gggatagagc cagataagaa aactaaatat 1740

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 1800

1741 tccctaaaac ggctaaagcc ggataaccaa ttttttactc gactaaattg tttttaaatt 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 1860

1801 gcgcttaatt aagacacctt acacacagtc aatcccacac ctttcagggg tccgaggggt 1860

.......... ......... pBABE_3_pri ..... SV40_enh SV40_pro ......

.......... .......... .......... .......... .......... ..........

1861 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 1920

1861 cgtccgtctt catacgtttc gtacgtagag ttaatcagtc gttggtccac acctttcagg 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 1980

1921 ggtccgaggg gtcgtccgtc ttcatacgtt tcgtacgtag agttaatcag tcgttggtat 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 2040

1981 cagggcgggg attgaggcgg gtagggcggg gattgaggcg ggtcaaggcg ggtaagaggc 2040

.......... ...... SV40_enh SV40_pro ..... SV40_origin .........

.......... .......... .......... .......... .......... ..........

2041 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag 2100

2041 ggggtaccga ctgattaaaa aaaataaata cgtctccggc tccggcggag acggagactc 2100

....... SV40_origin SV40_pro SV40pro_F_pri ....... ..........

.......... .......... .......... StuI(2135) .......... SmaI(2159)

2101 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 2160

2101 gataaggtct tcatcactcc tccgaaaaaa cctccggatc cgaaaacgtt tttcgagggc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg 2220

2161 cctcgaacat ataggtaaaa gcctagacta gttctctgtc ctactcctag caaagcgtac 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 2280

2221 taacttgttc tacctaacgt gcgtccaaga ggccggcgaa cccacctctc cgataagccg 2280

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 2340

2281 atactgaccc gtgttgtctg ttagccgacg agactacggc ggcacaaggc cgacagtcgc 2340

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag 2400

2341 gtccccgcgg gccaagaaaa acagttctgg ctggacaggc cacgggactt acttgacgtc 2400

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... MscI(2428) .......... .......... ..........

2401 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 2460

2401 ctgctccgtc gcgccgatag caccgaccgg tgctgcccgc aaggaacgcg tcgacacgag 2460

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 2520

2461 ctgcaacagt gacttcgccc ttccctgacc gacgataacc cgcttcacgg ccccgtccta 2520

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 2580

2521 gaggacagta gagtggaacg aggacggctc tttcataggt agtaccgact acgttacgcc 2580

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 2640

2581 gccgacgtat gcgaactagg ccgatggacg ggtaagctgg tggttcgctt tgtagcgtag 2640

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 2700

2641 ctcgctcgtg catgagccta ccttcggcca gaacagctag tcctactaga cctgcttctc 2700

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc 2760

2701 gtagtccccg agcgcggtcg gcttgacaag cggtccgagt tccgcgcgta cgggctgccg 2760

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 2820

2761 ctcctagagc agcactgggt accgctacgg acgaacggct tatagtacca ccttttaccg 2820

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 2880

2821 gcgaaaagac ctaagtagct gacaccggcc gacccacacc gcctggcgat agtcctgtat 2880

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 2940

2881 cgcaaccgat gggcactata acgacttctc gaaccgccgc ttacccgact ggcgaaggag 2940

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 3000

2941 cacgaaatgc catagcggcg agggctaagc gtcgcgtagc ggaagatagc ggaagaactg 3000

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... ......... BstBI(3028) ......... .......... ..........

3001 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc 3060

3001 ctcaagaaga ctcgccctga gaccccaagc tttactggct ggttcgctgc gggttggacg 3060

ORF 1 Neomycin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 3120

3061 gtagtgctct aaagctaagg tggcggcgga agatactttc caacccgaag ccttagcaaa 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 3180

3121 aggccctgcg gccgacctac taggaggtcg cgcccctaga gtacgacctc aagaagcggg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 3240

3181 tggggttgaa caaataacgt cgaatattac caatgtttat ttcgttatcg tagtgtttaa 3240

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

3241 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa c tcatcaatg 3300

3241 agtgtttatt tcgtaaaaaa agtgacgtaa gatcaacacc aaacaggttt gagtagttac 3300

.......... ......... SV40_PA_term ........ EBV_rev_pri .........

.......... .......... .......... .......... .......... ..........

3301 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 3360

3301 atagaatagt acagacatat ggcagctgga gatcgatctc gaaccgcatt agtaccagta 3360

.......... .......... .......... .......... ....... M13_reverse_pri

.......... .......... .......... .......... .......... ..........

3361 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 3420

3361 tcgacaaagg acacacttta acaataggcg agtgttaagg tgtgttgtat gctcggcctt 3420

M13_reverse_pri M13_pUC_rev_pri ........ .......... lac_pro

.......... .......... .......... .......... .......... ..........

3421 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 3480

3421 cgtatttcac atttcggacc ccacggatta ctcactcgat tgagtgtaat taacgcaacg 3480

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 3540

3481 cgagtgacgg gcgaaaggtc agccctttgg acagcacggt cgacgtaatt acttagccgg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 3600

3541 ttgcgcgccc ctctccgcca aacgcataac ccgcgagaag gcgaaggagc gagtgactga 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 3660

3601 gcgacgcgag ccagcaagcc gacgccgctc gccatagtcg agtgagtttc cgccattatg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 3720

3661 ccaataggtg tcttagtccc ctattgcgtc ctttcttgta cactcgtttt ccggtcgttt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 3780

3721 tccggtcctt ggcatttttc cggcgcaacg accgcaaaaa ggtatccgag gcggggggac 3780

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3781 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 3840

3781 tgctcgtagt gtttttagct gcgagttcag tctccaccgc tttgggctgt cctgatattt 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 3900

3841 ctatggtccg caaaggggga ccttcgaggg agcacgcgag aggacaaggc tgggacggcg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 3960

3901 aatggcctat ggacaggcgg aaagagggaa gcccttcgca ccgcgaaaga gtatcgagtg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 4020

3961 cgacatccat agagtcaagc cacatccagc aagcgaggtt cgacccgaca cacgtgcttg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 4080

4021 gggggcaagt cgggctggcg acgcggaata ggccattgat agcagaactc aggttgggcc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 4140

4081 attctgtgct gaatagcggt gaccgtcgtc ggtgaccatt gtcctaatcg tctcgctcca 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 4200

4141 tacatccgcc acgatgtctc aagaacttca ccaccggatt gatgccgatg tgatcttctt 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 4260

4201 gtcataaacc atagacgcga gacgacttcg gtcaatggaa gcctttttct caaccatcga 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 4320

4261 gaactaggcc gtttgtttgg tggcgaccat cgccaccaaa aaaacaaacg ttcgtcgtct 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 4380

4321 aatgcgcgtc tttttttcct agagttcttc taggaaacta gaaaagatgc cccagactgc 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 4440

4381 gagtcacctt gcttttgagt gcaattccct aaaaccagta ctctaatagt ttttcctaga 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 4500

4441 agtggatcta ggaaaattta atttttactt caaaatttag ttagatttca tatatactca 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 4560

4501 tttgaaccag actgtcaatg gttacgaatt agtcactccg tggatagagt cgctagacag 4560

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4561 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 4620

4561 ataaagcaag taggtatcaa cggactgagg ggcagcacat ctattgatgc tatgccctcc 4620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 4680

4621 cgaatggtag accggggtca cgacgttact atggcgctct gggtgcgagt ggccgaggtc 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 4740

4681 taaatagtcg ttatttggtc ggtcggcctt cccggctcgc gtcttcacca ggacgttgaa 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 4800

4741 ataggcggag gtaggtcaga taattaacaa cggcccttcg atctcattca tcaagcggtc 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 4860

4801 aattatcaaa cgcgttgcaa caacggtaac gatgtccgta gcaccacagt gcgagcagca 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 4920

4861 aaccataccg aagtaagtcg aggccaaggg ttgctagttc cgctcaatgt actagggggt 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 4980

4921 acaacacgtt ttttcgccaa tcgaggaagc caggaggcta gcaacagtct tcattcaacc 4980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 5040

4981 ggcgtcacaa tagtgagtac caataccgtc gtgacgtatt aagagaatga cagtacggta 5040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 5100

5041 ggcattctac gaaaagacac tgaccactca tgagttggtt cagtaagact cttatcacat 5100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 5160

5101 acgccgctgg ctcaacgaga acgggccgca gttatgccct attatggcgc ggtgtatcgt 5160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 5220

5161 cttgaaattt tcacgagtag taaccttttg caagaagccc cgcttttgag agttcctaga 5220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 5280

5221 atggcgacaa ctctaggtca agctacattg ggtgagcacg tgggttgact agaagtcgta 5280

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 5340

5281 gaaaatgaaa gtggtcgcaa agacccactc gtttttgtcc ttccgtttta cggcgttttt 5340

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 5400

5341 tcccttattc ccgctgtgcc tttacaactt atgagtatga gaaggaaaaa gttataataa 5400

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 5460

5401 cttcgtaaat agtcccaata acagagtact cgcctatgta taaacttaca taaatctttt 5460

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc....... 5513

5461 tatttgttta tccccaaggc gcgtgtaaag gggcttttca cggtggactg cag....... 5513

.......... .......... .......... .......... .......... ..........