Sequence of pcDNA3.1

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Sequence of pcDNA3.1_Hygro(-)

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Sequence of pcDNA3.1/Hygro(-) : 5596 BP; 1303 A; 1454 C; 1428 G; 1411 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... NruI(208) .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

ApaI(913),XbaI(915) XhoI(921) NotI(927) .......... ..........

901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc 960

901 caaatttgcc cgggagatct gagctcgccg gcggtgacac gacctataga cgtcttaagg 960

.......... .......... .......... .......... .......... ..........

........ BamHI(977) KpnI(993),HindIII(995),AflII(998) ........

961 accacactgg actagtggat ccgagctcgg taccaagctt aagtttaaac cgctgatcag 1020

961 tggtgtgacc tgatcaccta ggctcgagcc atggttcgaa ttcaaatttg gcgactagtc 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 1080

1021 ggagctgaca cggaagatca acggtcggta gacaacaaac ggggaggggg cacggaagga 1080

BGH_rev_pri ........ ......... bGH_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 1140

1081 actgggacct tccacggtga gggtgacagg aaaggattat tttactcctt taacgtagcg 1140

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 1200

1141 taacagactc atccacagta agataagacc ccccacccca ccccgtcctg tcgttccccc 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg 1260

1201 tcctaaccct tctgttatcg tccgtacgac ccctacgcca cccgagatac cgaagactcc 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa 1320

1261 gcctttcttg gtcgaccccg agatccccca taggggtgcg cgggacatcg ccgcgtaatt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 1380

1321 cgcgccgccc acaccaccaa tgcgcgtcgc actggcgatg tgaacggtcg cgggatcgcg 1380

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 1440

1381 ggcgaggaaa gcgaaagaag ggaaggaaag agcggtgcaa gcggccgaaa ggggcagttc 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 1500

1441 gagatttagc ccccgaggga aatcccaagg ctaaatcacg aaatgccgtg gagctggggt 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 1560

1501 tttttgaact aatcccacta ccaagtgcat cacccggtag cgggactatc tgccaaaaag 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 1620

1561 cgggaaactg caacctcagg tgcaagaaat tatcacctga gaacaaggtt tgaccttgtt 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... MscI(1668) ..........

1621 cactcaaccc tatctcggtc tattcttttg atttataagg gattttggcc atttcggcct 1680

1621 gtgagttggg atagagccag ataagaaaac taaatattcc ctaaaaccgg taaagccgga 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt 1740

1681 taaccaattt tttactcgac taaattgttt ttaaattgcg cttaattaag acaccttaca 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 1800

1741 cacagtcaat cccacacctt tcaggggtcc gaggggtcgt ccgtcttcat acgtttcgta 1800

pBABE_3_pri ........ SV40_enh SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 1860

1801 cgtagagtta atcagtcgtt ggtccacacc tttcaggggt ccgaggggtc gtccgtcttc 1860

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 1920

1861 atacgtttcg tacgtagagt taatcagtcg ttggtatcag ggcggggatt gaggcgggta 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 1980

1921 gggcggggat tgaggcgggt caaggcgggt aagaggcggg gtaccgactg attaaaaaaa 1980

..... SV40_enh SV40_pro SV40_origin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg 2040

1981 ataaatacgt ctccggctcc ggcggagacg gagactcgat aaggtcttca tcactcctcc 2040

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

.......... StuI(2052) .......... SmaI(2076) .......... ..........

2041 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg 2100

2041 gaaaaaacct ccggatccga aaacgttttt cgagggccct cgaacatata ggtaaaagcc 2100

.......... .......... .......... ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

2101 atctgatcag cacgtgatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct 2160

2101 tagactagtc gtgcactact ttttcggact tgagtggcgc tgcagacagc tcttcaaaga 2160

.......... .......... ORF 2 ORF 2 hygroB ..........

.......... .......... .......... .......... .......... ..........

2161 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg 2220

2161 ctagcttttc aagctgtcgc agaggctgga ctacgtcgag agcctcccgc ttcttagagc 2220

.......... ...... ORF 2 ORF 2 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga 2280

2221 acgaaagtcg aagctacatc ctcccgcacc tatacaggac gcccatttat cgacgcggct 2280

.......... ...... ORF 2 ORF 2 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc 2340

2281 accaaagatg tttctagcaa tacaaatagc cgtgaaacgt agccggcgcg agggctaagg 2340

.......... ...... ORF 2 ORF 2 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc 2400

2341 ccttcacgaa ctgtaacccc ttaagtcgct ctcggactgg ataacgtaga gggcggcacg 2400

.......... ...... ORF 2 ORF 2 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt 2460

2401 tgtcccacag tgcaacgttc tggacggact ttggcttgac gggcgacaag acgtcggcca 2460

.......... ...... ORF 2 ORF 2 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc 2520

2461 gcgcctccgg tacctacgct agcgacgccg gctagaatcg gtctgctcgc ccaagccggg 2520

.......... ...... ORF 2 ORF 2 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc 2580

2521 taagcctggc gttccttagc cagttatgtg atgtaccgca ctaaagtata cgcgctaacg 2580

.......... ...... ORF 2 ORF 2 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc 2640

2581 actaggggta cacatagtga ccgtttgaca ctacctgctg tggcagtcac gcaggcagcg 2640

.......... ...... ORF 2 ORF 2 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt 2700

2641 cgtccgagag ctactcgact acgaaacccg gctcctgacg gggcttcagg ccgtggagca 2700

.......... ...... ORF 2 ORF 2 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat 2760

2701 cgtgcgccta aagccgaggt tgttacagga ctgcctgtta ccggcgtatt gtcgccagta 2760

.......... ...... ORF 2 ORF 2 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg 2820

2761 actgacctcg ctccgctaca agcccctaag ggttatgctc cagcggttgt agaagaagac 2820

.......... ...... ORF 2 ORF 2 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga 2880

2821 ctccggcacc aaccgaacat acctcgtcgt ctgcgcgatg aagctcgcct ccgtaggcct 2880

.......... ...... ORF 2 ORF 2 hygroB ...... .......... ..........

......... SacII(2897) ......... .......... .......... ..........

2881 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta 2940

2881 cgaacgtcct agcggcgccg aggcccgcat atacgaggcg taaccagaac tggttgagat 2940

.......... ......... ORF 2 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc 3000

2941 agtctcgaac caactgccgt taaagctact acgtcgaacc cgcgtcccag ctacgctgcg 3000

.......... ......... ORF 2 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc 3060

3001 ttagcaggct aggcctcggc cctgacagcc cgcatgtgtt tagcgggcgt cttcgcgccg 3060

.......... ......... ORF 2 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac 3120

3061 gcagacctgg ctaccgacac atcttcatga gcggctatca cctttggctg cggggtcgtg 3120

.......... ......... ORF 2 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tcgtccgagg gcaaaggaat agcacgtgct acgagatttc gattccaccg ccgccttcta 3180

3121 agcaggctcc cgtttcctta tcgtgcacga tgctctaaag ctaaggtggc ggcggaagat 3180

hygroB ORF 2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 3240

3181 actttccaac ccgaagcctt agcaaaaggc cctgcggccg acctactagg aggtcgcgcc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 3300

3241 cctagagtac gacctcaaga agcgggtggg gttgaacaaa taacgtcgaa tattaccaat 3300

.......... .......... .......... ......... SV40_PA_term .........

.......... .......... .......... .......... .......... ..........

3301 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 3360

3301 gtttatttcg ttatcgtagt gtttaaagtg tttatttcgt aaaaaaagtg acgtaagatc 3360

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 3420

3361 aacaccaaac aggtttgagt agttacatag aatagtacag acatatggca gctggagatc 3420

... SV40_PA_term EBV_rev_pri ... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 3480

3421 gatctcgaac cgcattagta ccagtatcga caaaggacac actttaacaa taggcgagtg 3480

.......... ....... M13_reverse_pri .... M13_pUC_rev_pri .......

.......... .......... .......... .......... .......... ..........

3481 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 3540

3481 ttaaggtgtg ttgtatgctc ggccttcgta tttcacattt cggaccccac ggattactca 3540

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 3600

3541 ctcgattgag tgtaattaac gcaacgcgag tgacgggcga aaggtcagcc ctttggacag 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 3660

3601 cacggtcgac gtaattactt agccggttgc gcgcccctct ccgccaaacg cataacccgc 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 3720

3661 gagaaggcga aggagcgagt gactgagcga cgcgagccag caagccgacg ccgctcgcca 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 3780

3721 tagtcgagtg agtttccgcc attatgccaa taggtgtctt agtcccctat tgcgtccttt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 3840

3781 cttgtacact cgttttccgg tcgttttccg gtccttggca tttttccggc gcaacgaccg 3840

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

3841 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 3900

3841 caaaaaggta tccgaggcgg ggggactgct cgtagtgttt ttagctgcga gttcagtctc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 3960

3901 caccgctttg ggctgtcctg atatttctat ggtccgcaaa gggggacctt cgagggagca 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 4020

3961 cgcgagagga caaggctggg acggcgaatg gcctatggac aggcggaaag agggaagccc 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 4080

4021 ttcgcaccgc gaaagagtat cgagtgcgac atccatagag tcaagccaca tccagcaagc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 4140

4081 gaggttcgac ccgacacacg tgcttggggg gcaagtcggg ctggcgacgc ggaataggcc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 4200

4141 attgatagca gaactcaggt tgggccattc tgtgctgaat agcggtgacc gtcgtcggtg 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 4260

4201 accattgtcc taatcgtctc gctccataca tccgccacga tgtctcaaga acttcaccac 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 4320

4261 cggattgatg ccgatgtgat cttcttgtca taaaccatag acgcgagacg acttcggtca 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 4380

4321 atggaagcct ttttctcaac catcgagaac taggccgttt gtttggtggc gaccatcgcc 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 4440

4381 aaaaaaacaa acgttcgtcg tctaatgcgc gtcttttttt cctagagttc ttctaggaaa 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 4500

4441 ctagaaaaga tgccccagac tgcgagtcac cttgcttttg agtgcaattc cctaaaacca 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 4560

4501 gtactctaat agtttttcct agaagtggat ctaggaaaat ttaattttta cttcaaaatt 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 4620

4561 tagttagatt tcatatatac tcatttgaac cagactgtca atggttacga attagtcact 4620

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

4621 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 4680

4621 ccgtggatag agtcgctaga cagataaagc aagtaggtat caacggactg aggggcagca 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 4740

4681 catctattga tgctatgccc tcccgaatgg tagaccgggg tcacgacgtt actatggcgc 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 4800

4741 tctgggtgcg agtggccgag gtctaaatag tcgttatttg gtcggtcggc cttcccggct 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 4860

4801 cgcgtcttca ccaggacgtt gaaataggcg gaggtaggtc agataattaa caacggccct 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... FspI(4895) .......... ..........

4861 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 4920

4861 tcgatctcat tcatcaagcg gtcaattatc aaacgcgttg caacaacggt aacgatgtcc 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 4980

4921 gtagcaccac agtgcgagca gcaaaccata ccgaagtaag tcgaggccaa gggttgctag 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 5040

4981 ttccgctcaa tgtactaggg ggtacaacac gttttttcgc caatcgagga agccaggagg 5040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 5100

5041 ctagcaacag tcttcattca accggcgtca caatagtgag taccaatacc gtcgtgacgt 5100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 5160

5101 attaagagaa tgacagtacg gtaggcattc tacgaaaaga cactgaccac tcatgagttg 5160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 5220

5161 gttcagtaag actcttatca catacgccgc tggctcaacg agaacgggcc gcagttatgc 5220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 5280

5221 cctattatgg cgcggtgtat cgtcttgaaa ttttcacgag tagtaacctt ttgcaagaag 5280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 5340

5281 ccccgctttt gagagttcct agaatggcga caactctagg tcaagctaca ttgggtgagc 5340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 5400

5341 acgtgggttg actagaagtc gtagaaaatg aaagtggtcg caaagaccca ctcgtttttg 5400

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 5460

5401 tccttccgtt ttacggcgtt ttttccctta ttcccgctgt gcctttacaa cttatgagta 5460

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 5520

5461 tgagaaggaa aaagttataa taacttcgta aatagtccca ataacagagt actcgcctat 5520

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

5521 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 5580

5521 gtataaactt acataaatct ttttatttgt ttatccccaa ggcgcgtgta aaggggcttt 5580

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 agtgccacct gacgtc.... .......... .......... .......... .......... 5596

5581 tcacggtgga ctgcag.... .......... .......... .......... .......... 5596

.......... .......... .......... .......... .......... ..........