Sequence of pcDNA3.1_NT-GFP

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Sequence of pcDNA3.1_NT-GFP_Fusion

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Sequence of pcDNA3.1/NT-GFP Fusion : 6160 BP; 1476 A; 1571 C; 1542 G; 1571 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccagctgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... NruI(208) .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctg ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctaga 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatct 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

.......... .......... .......... .......... .......... ..........

901 caccatggcc agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt 960

901 gtggtaccgg tcgtttcctc ttcttgaaaa gtgacctcaa cagggttaag aacaacttaa 960

.......... .......... ........ ORF 2 GFP_ORF GFP_R_pri ........

.......... .......... .......... .......... .......... ..........

961 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgctac 1020

961 tctaccacta caattacccg tgtttaaaag acagtcacct ctcccacttc cactacgatg 1020

.......... ..... ORF 2 GFP_ORF GFPfusionREV_pri ..... ..........

HindIII(1028) ...... .......... .......... .......... ..........

1021 atacggaaag cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc 1080

1021 tatgcctttc gaatgggaat ttaaataaac gtgatgacct tttgatggac aaggtaccgg 1080

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aacacttgtc actactttct cttatggtgt tcaatgcttt tcccgttatc cggatcatat 1140

1081 ttgtgaacag tgatgaaaga gaataccaca agttacgaaa agggcaatag gcctagtata 1140

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat 1200

1141 ctttgccgta ctgaaaaagt tctcacggta cgggcttcca atacatgtcc ttgcgtgata 1200

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atctttcaaa gatgacggga actacaagac gcgtgctgaa gtcaagtttg aaggtgatac 1260

1201 tagaaagttt ctactgccct tgatgttctg cgcacgactt cagttcaaac ttccactatg 1260

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccttgttaat cgtatcgagt taaaaggtat tgattttaaa gaagatggaa acattctcgg 1320

1261 ggaacaatta gcatagctca attttccata actaaaattt cttctacctt tgtaagagcc 1320

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 acacaaactc gagtacaact ataactcaca caatgtatac atcacggcag acaaacaaaa 1380

1321 tgtgtttgag ctcatgttga tattgagtgt gttacatatg tagtgccgtc tgtttgtttt 1380

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gaatggaatc aaagctaact tcaaaattcg ccacaacatt gaagatggat ccgttcaact 1440

1381 cttaccttag tttcgattga agttttaagc ggtgttgtaa cttctaccta ggcaagttga 1440

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa 1500

1441 tcgtctggta atagttgttt tatgaggtta accgctaccg ggacaggaaa atggtctgtt 1500

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ccattacctg tcgacacaat ctgccctttc gaaagatccc aacgaaaagc gtgaccacat 1560

1501 ggtaatggac agctgtgtta gacgggaaag ctttctaggg ttgcttttcg cactggtgta 1560

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ggtccttctt gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa 1620

1561 ccaggaagaa ctcaaacatt gacgacgacc ctaatgtgta ccgtacctac tcgagatgtt 1620

........ GFP_F_pri ORF 2 GFP_ORF ........ .......... ..........

.... AgeI(1633) NheI(1639) KpnI(1649) .... .......... ..........

1621 aagcggttcc ggaccggtgc tagcggtacc gagctcggat ccactagtcc agtgtggtgg 1680

1621 ttcgccaagg cctggccacg atcgccatgg ctcgagccta ggtgatcagg tcacaccacc 1680

.......... .......... ORF 2 .......... .......... ..........

......... EcoRV(1692) NotI(1707) XbaI(1719) ApaI(1729) .........

1681 aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc gtttaaaccc 1740

1681 ttaagacgtc tataggtcgt gtcaccgccg gcgagctcag atctcccggg caaatttggg 1740

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg 1800

1741 cgactagtcg gagctgacac ggaagatcaa cggtcggtag acaacaaacg gggagggggc 1800

......... BGH_rev_pri ........ bGH_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

1801 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 1860

1801 acggaaggaa ctgggacctt ccacggtgag ggtgacagga aaggattatt ttactccttt 1860

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca 1920

1861 aacgtagcgt aacagactca tccacagtaa gataagaccc cccaccccac cccgtcctgt 1920

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg 1980

1921 cgttccccct cctaaccctt ctgttatcgt ccgtacgacc cctacgccac ccgagatacc 1980

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg 2040

1981 gaagactccg cctttcttgg tcgaccccga gatcccccat aggggtgcgc gggacatcgc 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 2100

2041 cgcgtaattc gcgccgccca caccaccaat gcgcgtcgca ctggcgatgt gaacggtcgc 2100

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 2160

2101 gggatcgcgg gcgaggaaag cgaaagaagg gaaggaaaga gcggtgcaag cggccgaaag 2160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc 2220

2161 gggcagttcg agatttagcc ccgtagggaa atcccaaggc taaatcacga aatgccgtgg 2220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 2280

2221 agctggggtt ttttgaacta atcccactac caagtgcatc acccggtagc gggactatct 2280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 2340

2281 gccaaaaagc gggaaactgc aacctcaggt gcaagaaatt atcacctgag aacaaggttt 2340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgggga 2400

2341 gaccttgttg tgagttggga tagagccaga taagaaaact aaatattccc taaaacccct 2400

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct 2460

2401 aaagccggat aaccaatttt ttactcgact aaattgtttt taaattgcgc ttaattaaga 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta 2520

2461 caccttacac acagtcaatc ccacaccttt caggggtccg aggggtccgt ccgtcttcat 2520

......... pBABE_3_pri ..... SV40_enh SV40_pro ...... ..........

.......... .......... .......... .......... .......... ..........

2521 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 2580

2521 acgtttcgta cgtagagtta atcagtcgtt ggtccacacc tttcaggggt ccgaggggtc 2580

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 2640

2581 gtccgtcttc atacgtttcg tacgtagagt taatcagtcg ttggtatcag ggcggggatt 2640

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 2700

2641 gaggcgggta gggcggggat tgaggcgggt caaggcgggt aagaggcggg gtaccgactg 2700

.......... ..... SV40_enh SV40_pro SV40_origin ..... ..........

.......... .......... .......... .......... .......... ..........

2701 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 2760

2701 attaaaaaaa ataaatacgt ctccggctcc ggcggagacg gagactcgat aaggtcttca 2760

.. SV40_origin SV40pro_F_pri SV40_pro .. .......... ..........

.......... .......... StuI(2782) .......... SmaI(2806) ..........

2761 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 2820

2761 tcactcctcc gaaaaaacct ccggatccga aaacgttttt cgagggccct cgaacatata 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg 2880

2821 ggtaaaagcc tagactagtt ctctgtccta ctcctagcaa agcgtactaa cttgttctac 2880

.......... .......... .......... .......... ........ ORF 3 Neomycin

.......... .......... .......... .......... .......... ..........

2881 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 2940

2881 ctaacgtgcg tccaagaggc cggcgaaccc acctctccga taagccgata ctgacccgtg 2940

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... NarI(2993)

2941 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 3000

2941 ttgtctgtta gccgacgaga ctacggcggc acaaggccga cagtcgcgtc cccgcgggcc 3000

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc 3060

3001 aagaaaaaca gttctggctg gacaggccac gggacttact tgacgtcctg ctccgtcgcg 3060

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 3120

3061 ccgatagcac cgaccggtgc tgcccgcaag gaacgcgtcg acacgagctg caacagtgac 3120

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 3180

3121 ttcgcccttc cctgaccgac gataacccgc ttcacggccc cgtcctagag gacagtagag 3180

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 3240

3181 tggaacgagg acggctcttt cataggtagt accgactacg ttacgccgcc gacgtatgcg 3240

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 3300

3241 aactaggccg atggacgggt aagctggtgg ttcgctttgt agcgtagctc gctcgtgcat 3300

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 3360

3301 gagcctacct tcggccagaa cagctagtcc tactagacct gcttctcgta gtccccgagc 3360

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg 3420

3361 gcggtcggct tgacaagcgg tccgagttcc gcgcgtacgg gctgccgctc ctagagcagc 3420

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 3480

3421 actgggtacc gctacggacg aacggcttat agtaccacct tttaccggcg aaaagaccta 3480

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 3540

3481 agtagctgac accggccgac ccacaccgcc tggcgatagt cctgtatcgc aaccgatggg 3540

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 3600

3541 cactataacg acttctcgaa ccgccgctta cccgactggc gaaggagcac gaaatgccat 3600

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag 3660

3601 agcggcgagg gctaagcgtc gcgtagcgga agatagcgga agaactgctc aagaagactc 3660

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt 3720

3661 gccctgagac cccaagcttt actggctggt tcgctgcggg ttggacggta gtgctctaaa 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg 3780

3721 gctaaggtgg cggcggaaga tactttccaa cccgaagcct tagcaaaagg ccctgcggcc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt 3840

3781 gacctactag gaggtcgcgc ccctagagta cgacctcaag aagcgggtgg ggttgaacaa 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 3900

3841 ataacgtcga atattaccaa tgtttatttc gttatcgtag tgtttaaagt gtttatttcg 3900

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 3960

3901 taaaaaaagt gacgtaagat caacaccaaa caggtttgag tagttacata gaatagtaca 3960

.......... ........ SV40_PA_term EBV_rev_pri ........ ..........

.......... .......... .......... .......... .......... ..........

3961 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 4020

3961 gacatatggc agctggagat cgatctcgaa ccgcattagt accagtatcg acaaaggaca 4020

.......... .......... .......... ....... M13_reverse_pri .......

.......... .......... .......... .......... .......... ..........

4021 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 4080

4021 cactttaaca ataggcgagt gttaaggtgt gttgtatgct cggccttcgt atttcacatt 4080

M13_pUC_rev_pri .... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

4081 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 4140

4081 tcggacccca cggattactc actcgattga gtgtaattaa cgcaacgcga gtgacgggcg 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 4200

4141 aaaggtcagc cctttggaca gcacggtcga cgtaattact tagccggttg cgcgcccctc 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 4260

4201 tccgccaaac gcataacccg cgagaaggcg aaggagcgag tgactgagcg acgcgagcca 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 4320

4261 gcaagccgac gccgctcgcc atagtcgagt gagtttccgc cattatgcca ataggtgtct 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 4380

4321 tagtccccta ttgcgtcctt tcttgtacac tcgttttccg gtcgttttcc ggtccttggc 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 4440

4381 atttttccgg cgcaacgacc gcaaaaaggt atccgaggcg gggggactgc tcgtagtgtt 4440

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

4441 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 4500

4441 tttagctgcg agttcagtct ccaccgcttt gggctgtcct gatatttcta tggtccgcaa 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 4560

4501 agggggacct tcgagggagc acgcgagagg acaaggctgg gacggcgaat ggcctatgga 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct 4620

4561 caggcggaaa gagggaagcc cttcgcaccg cgaaagagtt acgagtgcga catccataga 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 4680

4621 gtcaagccac atccagcaag cgaggttcga cccgacacac gtgcttgggg ggcaagtcgg 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 4740

4681 gctggcgacg cggaataggc cattgatagc agaactcagg ttgggccatt ctgtgctgaa 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 4800

4741 tagcggtgac cgtcgtcggt gaccattgtc ctaatcgtct cgctccatac atccgccacg 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 4860

4801 atgtctcaag aacttcacca ccggattgat gccgatgtga tcttcctgtc ataaaccata 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 4920

4861 gacgcgagac gacttcggtc aatggaagcc tttttctcaa ccatcgagaa ctaggccgtt 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 4980

4921 tgtttggtgg cgaccatcgc caccaaaaaa acaaacgttc gtcgtctaat gcgcgtcttt 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 5040

4981 ttttcctaga gttcttctag gaaactagaa aagatgcccc agactgcgag tcaccttgct 5040

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 5100

5041 tttgagtgca attccctaaa accagtactc taatagtttt tcctagaagt ggatctagga 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 5160

5101 aaatttaatt tttacttcaa aatttagtta gatttcatat atactcattt gaaccagact 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 5220

5161 gtcaatggtt acgaattagt cactccgtgg atagagtcgc tagacagata aagcaagtag 5220

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

5221 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 5280

5221 gtatcaacgg actgaggggc agcacatcta ttgatgctat gccctcccga atggtagacc 5280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 5340

5281 ggggtcacga cgttactatg gcgctctggg tgcgagtggc cgaggtctaa atagtcgtta 5340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 5400

5341 tttggtcggt cggccttccc ggctcgcgtc ttcaccagga cgttgaaata ggcggaggta 5400

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 5460

5401 ggtcagataa ttaacaacgg cccttcgatc tcattcatca agcggtcaat tatcaaacgc 5460

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 5520

5461 gttgcaacaa cggtaacgat gtccgtagca ccacagtgcg agcagcaaac cataccgaag 5520

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 5580

5521 taagtcgagg ccaagggttg ctagttccgc tcaatgtact agggggtaca acacgttttt 5580

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 5640

5581 tcgccaatcg aggaagccag gaggctagca acagtcttca ttcaaccggc gtcacaatag 5640

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 5700

5641 tgagtaccaa taccgtcgtg acgtattaag agaatgacag tacggtaggc attctacgaa 5700

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 5760

5701 aagacactga ccactcatga gttggttcag taagactctt atcacatacg ccgctggctc 5760

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 5820

5761 aacgagaacg ggccgcagtt atgccctatt atggcgcggt gtatcgtctt gaaattttca 5820

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 5880

5821 cgagtagtaa ccttttgcaa gaagccccgc ttttgagagt tcctagaatg gcgacaactc 5880

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 5940

5881 taggtcaagc tacattgggt gagcacgtgg gttgactaga agtcgtagaa aatgaaagtg 5940

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 6000

5941 gtcgcaaaga cccactcgtt tttgtccttc cgttttacgg cgttttttcc cttattcccg 6000

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 6060

6001 ctgtgccttt acaacttatg agtatgagaa ggaaaaagtt ataataactt cgtaaatagt 6060

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 6120

6061 cccaataaca gagtactcgc ctatgtataa acttacataa atctttttat ttgtttatcc 6120

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc .......... .......... 6160

6121 ccaaggcgcg tgtaaagggg cttttcacgg tggactgcag .......... .......... 6160

.......... .......... .......... .......... .......... ..........