Sequence of pcDNA3.1

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Sequence of pcDNA3.1_Zeo(+)

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Sequence of pcDNA3.1/Zeo(+) : 5015 BP; 1167 A; 1297 C; 1279 G; 1272 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccagctgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... NruI(208) .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctg ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

AflII(908),HindIII(911) KpnI(921) BamHI(929) ...... ..........

901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc 960

901 caaatttgaa ttcgaaccat ggctcgagcc taggtgatca ggtcacacca ccttaagacg 960

.......... .......... .......... .......... .......... ..........

PstI,EcoRV NotI79) XhoI(985) XbaI(991) ApaI(1001)

961 agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca 1020

961 tctataggtc gtgtcaccgc cggcgagctc agatctcccg ggcaaatttg ggcgactagt 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 1080

1021 cggagctgac acggaagatc aacggtcggt agacaacaaa cggggagggg gcacggaagg 1080

BGH_rev_pri ........ ......... bGH_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

1081 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 1140

1081 aactgggacc ttccacggtg agggtgacag gaaaggatta ttttactcct ttaacgtagc 1140

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 1200

1141 gtaacagact catccacagt aagataagac cccccacccc accccgtcct gtcgttcccc 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 1260

1201 ctcctaaccc ttctgttatc gtccgtacga cccctacgcc acccgagata ccgaagactc 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta 1320

1261 cgcctttctt ggtcgacccc gagatccccc ataggggtgc gcgggacatc gccgcgtaat 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 1380

1321 tcgcgccgcc cacaccacca atgcgcgtcg cactggcgat gtgaacggtc gcgggatcgc 1380

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 1440

1381 gggcgaggaa agcgaaagaa gggaaggaaa gagcggtgca agcggccgaa aggggcagtt 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc 1500

1441 cgagatttag ccccgtaggg aaatcccaag gctaaatcac gaaatgccgt ggagctgggg 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 1560

1501 ttttttgaac taatcccact accaagtgca tcacccggta gcgggactat ctgccaaaaa 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 1620

1561 gcgggaaact gcaacctcag gtgcaagaaa ttatcacctg agaacaaggt ttgaccttgt 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc 1680

1621 tgtgagttgg gatagagcca gataagaaaa ctaaatattc cctaaaaccc ctaaagccgg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg 1740

1681 ataaccaatt ttttactcga ctaaattgtt tttaaattgc gcttaattaa gacaccttac 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc 1800

1741 acacagtcaa tcccacacct ttcaggggtc cgaggggtcc gtccgtcttc atacgtttcg 1800

pBABE_3_pri ........ SV40_enh SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 1860

1801 tacgtagagt taatcagtcg ttggtccaca cctttcaggg gtccgagggg tcgtccgtct 1860

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 1920

1861 tcatacgttt cgtacgtaga gttaatcagt cgttggtatc agggcgggga ttgaggcggg 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 1980

1921 tagggcgggg attgaggcgg gtcaaggcgg gtaagaggcg gggtaccgac tgattaaaaa 1980

..... SV40_enh SV40_pro SV40_origin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 2040

1981 aaataaatac gtctccggct ccggcggaga cggagactcg ataaggtctt catcactcct 2040

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 2100

2041 ccgaaaaaac ctccggatcc gaaaacgttt ttcgagggcc ctcgaacata taggtaaaag 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat 2160

2101 cctagactag tcgtgcacaa ctgttaatta gtagccgtat catatagccg tatcatatta 2160

.......... .......... .......... EM7_pro .......... ..........

.......... .......... MscI(2187) .......... .......... ..........

2161 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg 2220

2161 tgctgttcca ctccttgatt tggtaccggt tcaactggtc acggcaaggc cacgagtggc 2220

.......... EM7_pro .......... ......... bleo sh_ble .........

.......... .......... .......... .......... .......... ..........

2221 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact 2280

2221 gcgcgctgca gcggcctcgc cagctcaaga cctggctggc cgagcccaag agggccctga 2280

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg 2340

2281 agcacctcct gctgaagcgg ccacaccagg ccctgctgca ctgggacaag tagtcgcgcc 2340

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg 2400

2341 aggtcctggt ccaccacggc ctgttgtggg accggaccca cacccacgcg ccggacctgc 2400

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg 2460

2401 tcgacatgcg gctcaccagc ctccagcaca ggtgcttgaa ggccctgcgg aggcccggcc 2460

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg 2520

2461 ggtactggct ctagccgctc gtcggcaccc ccgccctcaa gcgggacgcg ctgggccggc 2520

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt 2580

2521 cgttgacgca cgtgaagcac cggctcctcg tcctgactgt gcacgatgct ctaaagctaa 2580

......... bleo sh_ble ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga 2640

2581 ggtggcggcg gaagatactt tccaacccga agccttagca aaaggccctg cggccgacct 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg 2700

2641 actaggaggt cgcgccccta gagtacgacc tcaagaagcg ggtggggttg aacaaataac 2700

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

2701 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 2760

2701 gtcgaatatt accaatgttt atttcgttat cgtagtgttt aaagtgttta tttcgtaaaa 2760

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta 2820

2761 aaagtgacgt aagatcaaca ccaaacaggt ttgagtagtt acatagaata gtacagacat 2820

.......... ... SV40_PA_term EBV_rev_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 2880

2821 atggcagctg gagatcgatc tcgaaccgca ttagtaccag tatcgacaaa ggacacactt 2880

.......... .......... .......... .... M13_reverse_pri M13_pUC_rev_pri

.......... .......... .......... .......... .......... ..........

2881 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 2940

2881 taacaatagg cgagtgttaa ggtgtgttgt atgctcggcc ttcgtatttc acatttcgga 2940

M13_pUC_rev_pri .... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

2941 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 3000

2941 ccccacggat tactcactcg attgagtgta attaacgcaa cgcgagtgac gggcgaaagg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 3060

3001 tcagcccttt ggacagcacg gtcgacgtaa ttacttagcc ggttgcgcgc ccctctccgc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 3120

3061 caaacgcata acccgcgaga aggcgaagga gcgagtgact gagcgacgcg agccagcaag 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 3180

3121 ccgacgccgc tcgccatagt cgagtgagtt tccgccatta tgccaatagg tgtcttagtc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 3240

3181 ccctattgcg tcctttcttg tacactcgtt ttccggtcgt tttccggtcc ttggcatttt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 3300

3241 tccggcgcaa cgaccgcaaa aaggtatccg aggcgggggg actgctcgta gtgtttttag 3300

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3301 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 3360

3301 ctgcgagttc agtctccacc gctttgggct gtcctgatat ttctatggtc cgcaaagggg 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 3420

3361 gaccttcgag ggagcacgcg agaggacaag gctgggacgg cgaatggcct atggacaggc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt 3480

3421 ggaaagaggg aagcccttcg caccgcgaaa gagttacgag tgcgacatcc atagagtcaa 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 3540

3481 gccacatcca gcaagcgagg ttcgacccga cacacgtgct tggggggcaa gtcgggctgg 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 3600

3541 cgacgcggaa taggccattg atagcagaac tcaggttggg ccattctgtg ctgaatagcg 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 3660

3601 gtgaccgtcg tcggtgacca ttgtcctaat cgtctcgctc catacatccg ccacgatgtc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 3720

3661 tcaagaactt caccaccgga ttgatgccga tgtgatcttc ctgtcataaa ccatagacgc 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 3780

3721 gagacgactt cggtcaatgg aagccttttt ctcaaccatc gagaactagg ccgtttgttt 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 3840

3781 ggtggcgacc atcgccacca aaaaaacaaa cgttcgtcgt ctaatgcgcg tctttttttc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 3900

3841 ctagagttct tctaggaaac tagaaaagat gccccagact gcgagtcacc ttgcttttga 3900

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 3960

3901 gtgcaattcc ctaaaaccag tactctaata gtttttccta gaagtggatc taggaaaatt 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 4020

3961 taatttttac ttcaaaattt agttagattt catatatact catttgaacc agactgtcaa 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 4080

4021 tggttacgaa ttagtcactc cgtggataga gtcgctagac agataaagca agtaggtatc 4080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 4140

4081 aacggactga ggggcagcac atctattgat gctatgccct cccgaatggt agaccggggt 4140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 4200

4141 cacgacgtta ctatggcgct ctgggtgcga gtggccgagg tctaaatagt cgttatttgg 4200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 4260

4201 tcggtcggcc ttcccggctc gcgtcttcac caggacgttg aaataggcgg aggtaggtca 4260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... FspI(4314)

4261 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 4320

4261 gataattaac aacggccctt cgatctcatt catcaagcgg tcaattatca aacgcgttgc 4320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 4380

4321 aacaacggta acgatgtccg tagcaccaca gtgcgagcag caaaccatac cgaagtaagt 4380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 4440

4381 cgaggccaag ggttgctagt tccgctcaat gtactagggg gtacaacacg ttttttcgcc 4440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 4500

4441 aatcgaggaa gccaggaggc tagcaacagt cttcattcaa ccggcgtcac aatagtgagt 4500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 4560

4501 accaataccg tcgtgacgta ttaagagaat gacagtacgg taggcattct acgaaaagac 4560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 4620

4561 actgaccact catgagttgg ttcagtaaga ctcttatcac atacgccgct ggctcaacga 4620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 4680

4621 gaacgggccg cagttatgcc ctattatggc gcggtgtatc gtcttgaaat tttcacgagt 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 4740

4681 agtaaccttt tgcaagaagc cccgcttttg agagttccta gaatggcgac aactctaggt 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 4800

4741 caagctacat tgggtgagca cgtgggttga ctagaagtcg tagaaaatga aagtggtcgc 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 4860

4801 aaagacccac tcgtttttgt ccttccgttt tacggcgttt tttcccttat tcccgctgtg 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 4920

4861 cctttacaac ttatgagtat gagaaggaaa aagttataat aacttcgtaa atagtcccaa 4920

ORF 2 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 4980

4921 taacagagta ctcgcctatg tataaactta cataaatctt tttatttgtt tatccccaag 4980

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cgcgcacatt tccccgaaaa gtgccacctg acgtc..... .......... .......... 5015

4981 gcgcgtgtaa aggggctttt cacggtggac tgcag..... .......... .......... 5015

.......... .......... .......... .......... .......... ..........