Sequence of pcDNA3.1

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Sequence of pcDNA3.1_Zeo(-)

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Sequence of pcDNA3.1/Zeo(-) : 5014 BP; 1169 A; 1300 C; 1275 G; 1270 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccagctgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... NruI(208) .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctg ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

ApaI913),XbaI(915) XhoI(921) NotI(927) EcoRV(946) ..........

901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc 960

901 caaatttgcc cgggagatct gagctcgccg gcggtgacac gacctataga cgtcttaagg 960

.......... .......... .......... .......... .......... ..........

........ BamHI(977) KpnI(993),HindIII(995),AflII(998) ........

961 accacactgg actagtggat ccgagctcgg taccaagctt aagtttaaac cgctgatcag 1020

961 tggtgtgacc tgatcaccta ggctcgagcc atggttcgaa ttcaaatttg gcgactagtc 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 1080

1021 ggagctgaca cggaagatca acggtcggta gacaacaaac ggggaggggg cacggaagga 1080

BGH_rev_pri ........ ......... bGH_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 1140

1081 actgggacct tccacggtga gggtgacagg aaaggattat tttactcctt taacgtagcg 1140

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 1200

1141 taacagactc atccacagta agataagacc ccccacccca ccccgtcctg tcgttccccc 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg 1260

1201 tcctaaccct tctgttatcg tccgtacgac ccctacgcca cccgagatac cgaagactcc 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa 1320

1261 gcctttcttg gtcgaccccg agatccccca taggggtgcg cgggacatcg ccgcgtaatt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 1380

1321 cgcgccgccc acaccaccaa tgcgcgtcgc actggcgatg tgaacggtcg cgggatcgcg 1380

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 1440

1381 ggcgaggaaa gcgaaagaag ggaaggaaag agcggtgcaa gcggccgaaa ggggcagttc 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 1500

1441 gagatttagc cccgtaggga aatcccaagg ctaaatcacg aaatgccgtg gagctggggt 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 1560

1501 tttttgaact aatcccacta ccaagtgcat cacccggtag cgggactatc tgccaaaaag 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 1620

1561 cgggaaactg caacctcagg tgcaagaaat tatcacctga gaacaaggtt tgaccttgtt 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct 1680

1621 gtgagttggg atagagccag ataagaaaac taaatattcc ctaaaacccc taaagccgga 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt 1740

1681 taaccaattt tttactcgac taaattgttt ttaaattgcg cttaattaag acaccttaca 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca 1800

1741 cacagtcaat cccacacctt tcaggggtcc gaggggtccg tccgtcttca tacgtttcgt 1800

pBABE_3_pri ........ SV40_enh SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 1860

1801 acgtagagtt aatcagtcgt tggtccacac ctttcagggg tccgaggggt cgtccgtctt 1860

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 1920

1861 catacgtttc gtacgtagag ttaatcagtc gttggtatca gggcggggat tgaggcgggt 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 1980

1921 agggcgggga ttgaggcggg tcaaggcggg taagaggcgg ggtaccgact gattaaaaaa 1980

..... SV40_enh SV40_pro SV40_origin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 2040

1981 aataaatacg tctccggctc cggcggagac ggagactcga taaggtcttc atcactcctc 2040

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 2100

2041 cgaaaaaacc tccggatccg aaaacgtttt tcgagggccc tcgaacatat aggtaaaagc 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata 2160

2101 ctagactagt cgtgcacaac tgttaattag tagccgtatc atatagccgt atcatattat 2160

.......... .......... .......... EM7_pro .......... ..........

.......... .......... MscI(2186) .......... .......... ..........

2161 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc 2220

2161 gctgttccac tccttgattt ggtaccggtt caactggtca cggcaaggcc acgagtggcg 2220

.......... EM7_pro .......... ......... bleo sh_ble .........

.......... .......... .......... .......... .......... ..........

2221 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt 2280

2221 cgcgctgcag cggcctcgcc agctcaagac ctggctggcc gagcccaaga gggccctgaa 2280

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt 2340

2281 gcacctcctg ctgaagcggc cacaccaggc cctgctgcac tgggacaagt agtcgcgcca 2340

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga 2400

2341 ggtcctggtc caccacggcc tgttgtggga ccggacccac acccacgcgc cggacctgct 2400

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc 2460

2401 cgacatgcgg ctcaccagcc tccagcacag gtgcttgaag gccctgcgga ggcccggccg 2460

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg 2520

2461 gtactggctc tagccgctcg tcggcacccc cgccctcaag cgggacgcgc tgggccggcc 2520

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc 2580

2521 gttgacgcac gtgaagcacc ggctcctcgt cctgactgtg cacgatgctc taaagctaag 2580

......... bleo sh_ble ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 2640

2581 gtggcggcgg aagatacttt ccaacccgaa gccttagcaa aaggccctgc ggccgaccta 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 2700

2641 ctaggaggtc gcgcccctag agtacgacct caagaagcgg gtggggttga acaaataacg 2700

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

2701 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 2760

2701 tcgaatatta ccaatgttta tttcgttatc gtagtgttta aagtgtttat ttcgtaaaaa 2760

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 2820

2761 aagtgacgta agatcaacac caaacaggtt tgagtagtta catagaatag tacagacata 2820

.......... ... SV40_PA_term EBV_rev_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 2880

2821 tggcagctgg agatcgatct cgaaccgcat tagtaccagt atcgacaaag gacacacttt 2880

.......... .......... .......... .... M13_reverse_pri M13_pUC_rev_pri

.......... .......... .......... .......... .......... ..........

2881 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 2940

2881 aacaataggc gagtgttaag gtgtgttgta tgctcggcct tcgtatttca catttcggac 2940

M13_pUC_rev_pri .... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 3000

2941 cccacggatt actcactcga ttgagtgtaa ttaacgcaac gcgagtgacg ggcgaaaggt 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 3060

3001 cagccctttg gacagcacgg tcgacgtaat tacttagccg gttgcgcgcc cctctccgcc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 3120

3061 aaacgcataa cccgcgagaa ggcgaaggag cgagtgactg agcgacgcga gccagcaagc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 3180

3121 cgacgccgct cgccatagtc gagtgagttt ccgccattat gccaataggt gtcttagtcc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 3240

3181 cctattgcgt cctttcttgt acactcgttt tccggtcgtt ttccggtcct tggcattttt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 3300

3241 ccggcgcaac gaccgcaaaa aggtatccga ggcgggggga ctgctcgtag tgtttttagc 3300

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3301 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 3360

3301 tgcgagttca gtctccaccg ctttgggctg tcctgatatt tctatggtcc gcaaaggggg 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 3420

3361 accttcgagg gagcacgcga gaggacaagg ctgggacggc gaatggccta tggacaggcg 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc 3480

3421 gaaagaggga agcccttcgc accgcgaaag agttacgagt gcgacatcca tagagtcaag 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 3540

3481 ccacatccag caagcgaggt tcgacccgac acacgtgctt ggggggcaag tcgggctggc 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 3600

3541 gacgcggaat aggccattga tagcagaact caggttgggc cattctgtgc tgaatagcgg 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 3660

3601 tgaccgtcgt cggtgaccat tgtcctaatc gtctcgctcc atacatccgc cacgatgtct 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 3720

3661 caagaacttc accaccggat tgatgccgat gtgatcttcc tgtcataaac catagacgcg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 3780

3721 agacgacttc ggtcaatgga agcctttttc tcaaccatcg agaactaggc cgtttgtttg 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 3840

3781 gtggcgacca tcgccaccaa aaaaacaaac gttcgtcgtc taatgcgcgt ctttttttcc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 3900

3841 tagagttctt ctaggaaact agaaaagatg ccccagactg cgagtcacct tgcttttgag 3900

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 3960

3901 tgcaattccc taaaaccagt actctaatag tttttcctag aagtggatct aggaaaattt 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 4020

3961 aatttttact tcaaaattta gttagatttc atatatactc atttgaacca gactgtcaat 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 4080

4021 ggttacgaat tagtcactcc gtggatagag tcgctagaca gataaagcaa gtaggtatca 4080

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 4140

4081 acggactgag gggcagcaca tctattgatg ctatgccctc ccgaatggta gaccggggtc 4140

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 4200

4141 acgacgttac tatggcgctc tgggtgcgag tggccgaggt ctaaatagtc gttatttggt 4200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 4260

4201 cggtcggcct tcccggctcg cgtcttcacc aggacgttga aataggcgga ggtaggtcag 4260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... FspI(4313)

4261 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 4320

4261 ataattaaca acggcccttc gatctcattc atcaagcggt caattatcaa acgcgttgca 4320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 4380

4321 acaacggtaa cgatgtccgt agcaccacag tgcgagcagc aaaccatacc gaagtaagtc 4380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 4440

4381 gaggccaagg gttgctagtt ccgctcaatg tactaggggg tacaacacgt tttttcgcca 4440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 4500

4441 atcgaggaag ccaggaggct agcaacagtc ttcattcaac cggcgtcaca atagtgagta 4500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 4560

4501 ccaataccgt cgtgacgtat taagagaatg acagtacggt aggcattcta cgaaaagaca 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 4620

4561 ctgaccactc atgagttggt tcagtaagac tcttatcaca tacgccgctg gctcaacgag 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 4680

4621 aacgggccgc agttatgccc tattatggcg cggtgtatcg tcttgaaatt ttcacgagta 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 4740

4681 gtaacctttt gcaagaagcc ccgcttttga gagttcctag aatggcgaca actctaggtc 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 4800

4741 aagctacatt gggtgagcac gtgggttgac tagaagtcgt agaaaatgaa agtggtcgca 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 4860

4801 aagacccact cgtttttgtc cttccgtttt acggcgtttt ttcccttatt cccgctgtgc 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 4920

4861 ctttacaact tatgagtatg agaaggaaaa agttataata acttcgtaaa tagtcccaat 4920

ORF 1 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 4980

4921 aacagagtac tcgcctatgt ataaacttac ataaatcttt ttatttgttt atccccaagg 4980

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gcgcacattt ccccgaaaag tgccacctga cgtc...... .......... .......... 5014

4981 cgcgtgtaaa ggggcttttc acggtggact gcag...... .......... .......... 5014

.......... .......... .......... .......... .......... ..........