t@t@3LOCUS pcDNA3.1/nV5-DEST 7136 bp DNA SYN 29-Aug-2007 DEFINITION pcDNA3.1/nV5-DEST ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..7136 /organism="pcDNA3.1/nV5-DEST" /mol_type="other DNA" misc_feature 315..602 /label="CAG_enhancer" promoter 236..852 /label="CMV_immearly_promoter" misc_feature 769..789 /label="CMV_fwd_primer" promoter 863..881 /label="T7_promoter" misc_feature 914..955 /label="V5" misc_feature 1007..1131 /label="attR1" misc_feature complement(1031..1131) /label="attR2" promoter 1156..1179 /label="lac_promoter" CDS 1240..1899 /label="CAT/CamR" /gene="CAT/CamR" /note="ORF frame 1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" gene 1240..1899 /label="CAT/CamR" /gene="CAT/CamR" misc_feature 2241..2546 /label="ccdB" misc_feature 2587..2687 /label="attR2" misc_feature complement(2587..2694) /label="attR1" misc_feature complement(2730..2747) /label="BGH_rev_primer" terminator 2733..2960 /label="bGH_PA_terminator" rep_origin 3023..3329 /label="f1_origin" misc_feature complement(3443..3463) /label="pBABE_3_primer" misc_feature complement(3449..3664) /label="SV40_enhancer" promoter 3461..3729 /label="SV40_promoter" rep_origin 3628..3705 /label="SV40_origin" misc_feature 3690..3709 /label="SV40pro_F_primer" CDS 3844..4638 /label="Neomycin" /gene="Neomycin" /note="ORF frame 1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene 3847..4635 /label="Neomycin" /gene="Neomycin" terminator 4815..4934 /label="SV40_PA_terminator" misc_feature 4903..4922 /label="EBV_rev_primer" misc_feature complement(4978..4996) /label="M13_reverse_primer" misc_feature complement(4995..5017) /label="M13_pUC_rev_primer" promoter complement(5031..5060) /label="lac_promoter" rep_origin complement(5369..5985) /label="pBR322_origin" CDS complement(6140..7000) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(6140..7000) /label="Ampicillin" /gene="Ampicillin" promoter complement(7042..7070) /label="AmpR_promoter" ORIGIN 1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGT 901 TAAGCTTACC ATGGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGGAAAA 961 CCTGTATTTT CAGGGCCCGG ATCCGTCGAC GAATTCTGCA GATATCACAA GTTTGTACAA 1021 AAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT ATATTAAATT AGATTTTGCA 1081 TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC CAGTCACTAT GGCGGCCGCA 1141 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATAATGTGTG GATTTTGAGT 1201 TAGGATCCGG CGAGATTTTC AGGAGCTAAG GAAGCTAAAA TGGAGAAAAA AATCACTGGA 1261 TATACCACCG TTGATATATC CCAATGGCAT CGTAAAGAAC ATTTTGAGGC ATTTCAGTCA 1321 GTTGCTCAAT GTACCTATAA CCAGACCGTT CAGCTGGATA TTACGGCCTT TTTAAAGACC 1381 GTAAAGAAAA ATAAGCACAA GTTTTATCCG GCCTTTATTC ACATTCTTGC CCGCCTGATG 1441 AATGCTCATC CGGAATTCCG TATGGCAATG AAAGACGGTG AGCTGGTGAT ATGGGATAGT 1501 GTTCACCCTT GTTACACCGT TTTCCATGAG CAAACTGAAA CGTTTTCATC GCTCTGGAGT 1561 GAATACCACG ACGATTTCCG GCAGTTTCTA CACATATATT CGCAAGATGT GGCGTGTTAC 1621 GGTGAAAACC TGGCCTATTT CCCTAAAGGG TTTATTGAGA ATATGTTTTT CGTCTCAGCC 1681 AATCCCTGGG TGAGTTTCAC CAGTTTTGAT TTAAACGTGG CCAATATGGA CAACTTCTTC 1741 GCCCCCGTTT TCACCATGGG CAAATATTAT ACGCAAGGCG ACAAGGTGCT GATGCCGCTG 1801 GCGATTCAGG TTCATCATGC CGTCTGTGAT GGCTTCCATG TCGGCAGAAT GCTTAATGAA 1861 TTACAACAGT ACTGCGATGA GTGGCAGGGC GGGGCGTAAA CGCGTGGATC CGGCTTACTA 1921 AAAGCCAGAT AACAGTATGC GTATTTGCGC GCTGATTTTT GCGGTATAAG AATATATACT 1981 GATATGTATA CCCGAAGTAT GTCAAAAAGA GGTGTGCTAT GAAGCAGCGT ATTACAGTGA 2041 CAGTTGACAG CGACAGCTAT CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTCT 2101 GGTAAGCACA ACCATGCAGA ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA 2161 AAATCAGGAA GGGATGGCTG AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCTGA 2221 CGAGAACAGG GACTGGTGAA ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT 2281 ATCGTCTGTT TGTGGATGTA CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA 2341 TCCCCCTGGC CAGTGCACGT CTGCTGTCAG ATAAAGTCTC CCGTGAACTT TACCCGGTGG 2401 TGCATATCGG GGATGAAAGC TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT 2461 CCGTTATCGG GGAAGAAGTG GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA 2521 TTAACCTGAT GTTCTGGGGA ATATAAATGT CAGGCTCCGT TATACACAGC CAGTCTGCAG 2581 GTCGACCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG TTTTTTATGC 2641 AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC AGCTTTCTTG 2701 TACAAAGTGG TGATAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC 2761 TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT 2821 TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG 2881 GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG 2941 GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CTAGGGGGTA 3001 TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT 3061 GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT 3121 CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG 3181 ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG 3241 TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA 3301 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA 3361 TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA 3421 ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 3481 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 3541 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 3601 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 3661 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC 3721 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 3781 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 3841 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 3901 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 3961 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 4021 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 4081 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 4141 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 4201 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 4261 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 4321 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 4381 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 4441 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG 4501 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 4561 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 4621 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 4681 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 4741 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 4801 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 4861 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 4921 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 4981 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 5041 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 5101 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 5161 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 5221 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 5281 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 5341 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 5401 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 5461 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 5521 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 5581 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 5641 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 5701 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 5761 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 5821 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 5881 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 5941 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 6001 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 6061 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 6121 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 6181 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 6241 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 6301 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 6361 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 6421 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 6481 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 6541 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 6601 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 6661 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 6721 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 6781 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 6841 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 6901 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 6961 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 7021 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 7081 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTC // pcDNA3.1/nV5-DEST "Times New Roman-t@C;ZCAG_enh"Times New Romant@CTCMV_immearly_pro"Times New Romant@CɆ CMV_fwd_pri"Times New Romant@_qT7_pro"Times New Romant@CV5"Times New Romant@CkattR1"Times New Romant@CɆkattR2"Times New Romant@lac_pro"Times New Romant@CkORF 1"Times New Romant@CkCAT/CamR"Times New Romant@CɆ ccdB"Times New Romant@  attR2"Times New Romant@C attR1"Times New Romant@C  BGH_rev_pri"Times New Romant@CɆ  bGH_PA_term"Times New Romant@   f1_origin"Times New Romant@Cs  pBABE_3_pri"Times New Romant@Cy PSV40_enh"Times New Roman(t@CɆ SV40_pro"Times New Romant@,y SV40_origin"Times New Romant@Cj} SV40pro_F_pri"Times New Roman)t@CORF 1"Times New Romant@CɆNeomycin"Times New Romant@F SV40_PA_term"Times New Romant@C': EBV_rev_pri"Times New Romant@CrM13_reverse_pri"Times New Romant@CɆM13_pUC_rev_pri"Times New Romant@lac_pro"Times New Romant@Ca pBR322_origin"Times New Romant@CXORF 2"Times New Romant@CɆX Ampicillin"Times New Romant@AmpR_pro"Times New Roman t@BglII"Times New Roman t`L=L t@NruI(208),SpeI(249)"Times New RomanwcQ35Q t@NdeI"Times New Roman (u0bSMb t@SacI(818),HindIII(902)"Times New Roman2Ygzzc t@ApaI(977),EcoRV(1003)"Times New Romanp t@NotI"Times New Romanm t@StuI"Times New Roman<Pcucr t@NarI"Times New Romanp0iCaUCUd t@BstBI"Times New Roman. t@test"Times New RomanP\V??@c t@test"Times New Roman S ^j(j(d t@test"Times New Roman?Seef t@test"Times New RomanV~