Sequence of pcDNA3.1

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Sequence of pcDNA3.1_nV5-DEST

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Sequence of pcDNA3.1/nV5-DEST : 7136 BP; 1734 A; 1764 C; 1780 G; 1858 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccagctgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

SpeI(249) .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctg ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatca 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

HindIII(902) ........ .......... .......... .......... ..........

901 taagcttacc atgggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacggaaaa 960

901 attcgaatgg tacccattcg gatagggatt gggagaggag ccagagctaa gatgcctttt 960

.......... .......... .......... V5 .......... ..........

.......... ApaI(977) .......... ......... EcoRV(1003) .........

961 cctgtatttt cagggcccgg atccgtcgac gaattctgca gatatcacaa gtttgtacaa 1020

961 ggacataaaa gtcccgggcc taggcagctg cttaagacgt ctatagtgtt caaacatgtt 1020

.......... .......... .......... .......... .......... attR1

.......... .......... .......... .......... .......... ..........

1021 aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca 1080

1021 ttttcgactt gctctttgca ttttactata tttatagtta tataatttaa tctaaaacgt 1080

.......... .......... attR1 attR2 .......... ..........

.......... .......... .......... .......... .......... NotI(1133)

1081 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca 1140

1081 attttttgtc tgatgtatta tgacattttg tgttgtatag gtcagtgata ccgccggcgt 1140

.......... ......... attR1 attR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt 1200

1141 aatccgtggg gtccgaaatg tgaaatacga aggccgagca tattacacac ctaaaactca 1200

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 taggatccgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga 1260

1201 atcctaggcc gctctaaaag tcctcgattc cttcgatttt acctcttttt ttagtgacct 1260

.......... .......... .......... ........ ORF 1 CAT/CamR ........

.......... .......... .......... .......... .......... ..........

1261 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca 1320

1261 atatggtggc aactatatag ggttaccgta gcatttcttg taaaactccg taaagtcagt 1320

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc 1380

1321 caacgagtta catggatatt ggtctggcaa gtcgacctat aatgccggaa aaatttctgg 1380

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg 1440

1381 catttctttt tattcgtgtt caaaataggc cggaaataag tgtaagaacg ggcggactac 1440

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt 1500

1441 ttacgagtag gccttaaggc ataccgttac tttctgccac tcgaccacta taccctatca 1500

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt 1560

1501 caagtgggaa caatgtggca aaaggtactc gtttgacttt gcaaaagtag cgagacctca 1560

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac 1620

1561 cttatggtgc tgctaaaggc cgtcaaagat gtgtatataa gcgttctaca ccgcacaatg 1620

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc 1680

1621 ccacttttgg accggataaa gggatttccc aaataactct tatacaaaaa gcagagtcgg 1680

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc 1740

1681 ttagggaccc actcaaagtg gtcaaaacta aatttgcacc ggttatacct gttgaagaag 1740

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg 1800

1741 cgggggcaaa agtggtaccc gtttataata tgcgttccgc tgttccacga ctacggcgac 1800

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa 1860

1801 cgctaagtcc aagtagtacg gcagacacta ccgaaggtac agccgtctta cgaattactt 1860

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta 1920

1861 aatgttgtca tgacgctact caccgtcccg ccccgcattt gcgcacctag gccgaatgat 1920

........ ORF 1 CAT/CamR ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact 1980

1921 tttcggtcta ttgtcatacg cataaacgcg cgactaaaaa cgccatattc ttatatatga 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat gaagcagcgt attacagtga 2040

1981 ctatacatat gggcttcata cagtttttct ccacacgata cttcgtcgca taatgtcact 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct 2100

2041 gtcaactgtc gctgtcgata gtcaacgagt tccgtatata ctacagttat agaggccaga 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga 2160

2101 ccattcgtgt tggtacgtct tacttcgggc agcagacgca cggcttgcga cctttcgcct 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga 2220

2161 tttagtcctt ccctaccgac tccagcgggc caaataactt tacttgccga gaaaacgact 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgagaacagg gactggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt 2280

2221 gctcttgtcc ctgaccactt tacgtcaaat tccaaatgtg gatattttct ctctcggcaa 2280

.......... .......... .......... ccdB .......... ..........

.......... .......... .......... .......... .......... ..........

2281 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga 2340

2281 tagcagacaa acacctacat gtctcactat aataactgtg cgggcccgct gcctaccact 2340

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg 2400

2341 agggggaccg gtcacgtgca gacgacagtc tatttcagag ggcacttgaa atgggccacc 2400

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct 2460

2401 acgtatagcc cctactttcg accgcgtact actggtggct ataccggtca cacggccaga 2460

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca 2520

2461 ggcaatagcc ccttcttcac cgactagagt cggtggcgct tttactgtag tttttgcggt 2520

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttaacctgat gttctgggga atataaatgt caggctccgt tatacacagc cagtctgcag 2580

2521 aattggacta caagacccct tatatttaca gtccgaggca atatgtgtcg gtcagacgtc 2580

.......... ccdB .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc 2640

2581 cagctggtat cactgaccta tacaacacaa aatgtcataa tacatcagac aaaaaatacg 2640

.......... .......... ......... attR2 attR1 ......... ..........

.......... .......... .......... .......... .......... ..........

2641 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg 2700

2641 ttttagatta aattatataa ctataaatat agtaaaatgc aaagagcaag tcgaaagaac 2700

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tacaaagtgg tgataaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc 2760

2701 atgtttcacc actatttggg cgactagtcg gagctgacac ggaagatcaa cggtcggtag 2760

.......... .......... ........ BGH_rev_pri bGH_PA_term ........

.......... .......... .......... .......... .......... ..........

2761 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 2820

2761 acaacaaacg gggagggggc acggaaggaa ctgggacctt ccacggtgag ggtgacagga 2820

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 2880

2821 aaggattatt ttactccttt aacgtagcgt aacagactca tccacagtaa gataagaccc 2880

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 2940

2881 cccaccccac cccgtcctgt cgttccccct cctaaccctt ctgttatcgt ccgtacgacc 2940

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta 3000

2941 cctacgccac ccgagatacc gaagactccg cctttcttgg tcgaccccga gatcccccat 3000

bGH_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 3060

3001 aggggtgcgc gggacatcgc cgcgtaattc gcgccgccca caccaccaat gcgcgtcgca 3060

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

3061 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 3120

3061 ctggcgatgt gaacggtcgc gggatcgcgg gcgaggaaag cgaaagaagg gaaggaaaga 3120

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 3180

3121 gcggtgcaag cggccgaaag gggcagttcg agatttagcc cccgagggaa atcccaaggc 3180

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 3240

3181 taaatcacga aatgccgtgg agctggggtt ttttgaacta atcccactac caagtgcatc 3240

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 3300

3241 acccggtagc gggactatct gccaaaaagc gggaaactgc aacctcaggt gcaagaaatt 3300

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 3360

3301 atcacctgag aacaaggttt gaccttgttg tgagttggga tagagccaga taagaaaact 3360

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 3420

3361 aaatattccc taaaacggct aaagccggat aaccaatttt ttactcgact aaattgtttt 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 3480

3421 taaattgcgc ttaattaaga caccttacac acagtcaatc ccacaccttt caggggtccg 3480

.......... .......... ..... pBABE_3_pri SV40_enh SV40_pro .....

.......... .......... .......... .......... .......... ..........

3481 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 3540

3481 aggggtcgtc cgtcttcata cgtttcgtac gtagagttaa tcagtcgttg gtccacacct 3540

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 3600

3541 ttcaggggtc cgaggggtcg tccgtcttca tacgtttcgt acgtagagtt aatcagtcgt 3600

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 3660

3601 tggtatcagg gcggggattg aggcgggtag ggcggggatt gaggcgggtc aaggcgggta 3660

.......... ...... SV40_enh SV40_pro ..... SV40_origin .........

.......... .......... .......... .......... .......... ..........

3661 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc 3720

3661 agaggcgggg taccgactga ttaaaaaaaa taaatacgtc tccggctccg gcggagacgg 3720

.......... ... SV40_pro SV40_origin SV40pro_F_pri ... ..........

.......... .......... .......... .......... StuI(3761) ..........

3721 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag 3780

3721 agactcgata aggtcttcat cactcctccg aaaaaacctc cggatccgaa aacgtttttc 3780

SV40_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt 3840

3781 gagggccctc gaacatatag gtaaaagcct agactagttc tctgtcctac tcctagcaaa 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 3900

3841 gcgtactaac ttgttctacc taacgtgcgt ccaagaggcc ggcgaaccca cctctccgat 3900

.......... .......... ........ ORF 1 Neomycin ........ ..........

.......... .......... .......... .......... .......... ..........

3901 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 3960

3901 aagccgatac tgacccgtgt tgtctgttag ccgacgagac tacggcggca caaggccgac 3960

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... NarI(3972) .......... .......... .......... ..........

3961 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 4020

3961 agtcgcgtcc ccgcgggcca agaaaaacag ttctggctgg acaggccacg ggacttactt 4020

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 4080

4021 gacgtcctgc tccgtcgcgc cgatagcacc gaccggtgct gcccgcaagg aacgcgtcga 4080

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 4140

4081 cacgagctgc aacagtgact tcgcccttcc ctgaccgacg ataacccgct tcacggcccc 4140

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 4200

4141 gtcctagagg acagtagagt ggaacgagga cggctctttc ataggtagta ccgactacgt 4200

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 4260

4201 tacgccgccg acgtatgcga actaggccga tggacgggta agctggtggt tcgctttgta 4260

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 4320

4261 gcgtagctcg ctcgtgcatg agcctacctt cggccagaac agctagtcct actagacctg 4320

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc 4380

4321 cttctcgtag tccccgagcg cggtcggctt gacaagcggt ccgagttccg cgcgtacggg 4380

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 4440

4381 ctgccgctcc tagagcagca ctgggtaccg ctacggacga acggcttata gtaccacctt 4440

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 4500

4441 ttaccggcga aaagacctaa gtagctgaca ccggccgacc cacaccgcct ggcgatagtc 4500

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 4560

4501 ctgtatcgca accgatgggc actataacga cttctcgaac cgccgcttac ccgactggcg 4560

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 4620

4561 aaggagcacg aaatgccata gcggcgaggg ctaagcgtcg cgtagcggaa gatagcggaa 4620

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... ......... BstBI(4654) ......... ..........

4621 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca 4680

4621 gaactgctca agaagactcg ccctgagacc ccaagcttta ctggctggtt cgctgcgggt 4680

ORF 1 Neomycin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 4740

4681 tggacggtag tgctctaaag ctaaggtggc ggcggaagat actttccaac ccgaagcctt 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 4800

4741 agcaaaaggc cctgcggccg acctactagg aggtcgcgcc cctagagtac gacctcaaga 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 4860

4801 agcgggtggg gttgaacaaa taacgtcgaa tattaccaat gtttatttcg ttatcgtagt 4860

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

4861 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 4920

4861 gtttaaagtg tttatttcgt aaaaaaagtg acgtaagatc aacaccaaac aggtttgagt 4920

.......... ......... SV40_PA_term ......... ......... EBV_rev_pri

.......... .......... .......... .......... .......... ..........

4921 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat 4980

4921 agttacatag aatagtacag acatatggca gctggagatc gatctcgaac cgcattagta 4980

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 5040

4981 ccagtatcga caaaggacac actttaacaa taggcgagtg ttaaggtgtg ttgtatgctc 5040

M13_reverse_pri . M13_pUC_rev_pri ....... .......... lac_pro

.......... .......... .......... .......... .......... ..........

5041 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 5100

5041 ggccttcgta tttcacattt cggaccccac ggattactca ctcgattgag tgtaattaac 5100

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 5160

5101 gcaacgcgag tgacgggcga aaggtcagcc ctttggacag cacggtcgac gtaattactt 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 5220

5161 agccggttgc gcgcccctct ccgccaaacg cataacccgc gagaaggcga aggagcgagt 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 5280

5221 gactgagcga cgcgagccag caagccgacg ccgctcgcca tagtcgagtg agtttccgcc 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 5340

5281 attatgccaa taggtgtctt agtcccctat tgcgtccttt cttgtacact cgttttccgg 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 5400

5341 tcgttttccg gtccttggca tttttccggc gcaacgaccg caaaaaggta tccgaggcgg 5400

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

5401 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 5460

5401 ggggactgct cgtagtgttt ttagctgcga gttcagtctc caccgctttg ggctgtcctg 5460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 5520

5461 atatttctat ggtccgcaaa gggggacctt cgagggagca cgcgagagga caaggctggg 5520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 5580

5521 acggcgaatg gcctatggac aggcggaaag agggaagccc ttcgcaccgc gaaagagtat 5580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 5640

5581 cgagtgcgac atccatagag tcaagccaca tccagcaagc gaggttcgac ccgacacacg 5640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5641 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 5700

5641 tgcttggggg gcaagtcggg ctggcgacgc ggaataggcc attgatagca gaactcaggt 5700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5701 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 5760

5701 tgggccattc tgtgctgaat agcggtgacc gtcgtcggtg accattgtcc taatcgtctc 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 5820

5761 gctccataca tccgccacga tgtctcaaga acttcaccac cggattgatg ccgatgtgat 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 5880

5821 cttcttgtca taaaccatag acgcgagacg acttcggtca atggaagcct ttttctcaac 5880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc 5940

5881 catcgagaac taggccgttt gtttggtggc gaccatcgcc aaaaaaacaa acgttcgtcg 5940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5941 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 6000

5941 tctaatgcgc gtcttttttt cctagagttc ttctaggaaa ctagaaaaga tgccccagac 6000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6001 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 6060

6001 tgcgagtcac cttgcttttg agtgcaattc cctaaaacca gtactctaat agtttttcct 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 6120

6061 agaagtggat ctaggaaaat ttaattttta cttcaaaatt tagttagatt tcatatatac 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 6180

6121 tcatttgaac cagactgtca atggttacga attagtcact ccgtggatag agtcgctaga 6180

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

6181 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 6240

6181 cagataaagc aagtaggtat caacggactg aggggcagca catctattga tgctatgccc 6240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 6300

6241 tcccgaatgg tagaccgggg tcacgacgtt actatggcgc tctgggtgcg agtggccgag 6300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 6360

6301 gtctaaatag tcgttatttg gtcggtcggc cttcccggct cgcgtcttca ccaggacgtt 6360

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 6420

6361 gaaataggcg gaggtaggtc agataattaa caacggccct tcgatctcat tcatcaagcg 6420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 6480

6421 gtcaattatc aaacgcgttg caacaacggt aacgatgtcc gtagcaccac agtgcgagca 6480

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 6540

6481 gcaaaccata ccgaagtaag tcgaggccaa gggttgctag ttccgctcaa tgtactaggg 6540

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 6600

6541 ggtacaacac gttttttcgc caatcgagga agccaggagg ctagcaacag tcttcattca 6600

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 6660

6601 accggcgtca caatagtgag taccaatacc gtcgtgacgt attaagagaa tgacagtacg 6660

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 6720

6661 gtaggcattc tacgaaaaga cactgaccac tcatgagttg gttcagtaag actcttatca 6720

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 6780

6721 catacgccgc tggctcaacg agaacgggcc gcagttatgc cctattatgg cgcggtgtat 6780

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 6840

6781 cgtcttgaaa ttttcacgag tagtaacctt ttgcaagaag ccccgctttt gagagttcct 6840

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 6900

6841 agaatggcga caactctagg tcaagctaca ttgggtgagc acgtgggttg actagaagtc 6900

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 6960

6901 gtagaaaatg aaagtggtcg caaagaccca ctcgtttttg tccttccgtt ttacggcgtt 6960

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 7020

6961 ttttccctta ttcccgctgt gcctttacaa cttatgagta tgagaaggaa aaagttataa 7020

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 7080

7021 taacttcgta aatagtccca ataacagagt actcgcctat gtataaactt acataaatct 7080

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

7081 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc.... 7136

7081 ttttatttgt ttatccccaa ggcgcgtgta aaggggcttt tcacggtgga ctgcag.... 7136

.......... .......... .......... .......... .......... ..........