Sequence of pcDNA31-His-A

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Sequence of pcDNA31-His-A

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Sequence of pcDNA3.1/His A : 5514 BP; 1274 A; 1425 C; 1413 G; 1402 T; 0 other;

.......... BglII(12) .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccacgtgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... NruI(208) .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

SpeI(249) .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ......... CMV_imm_pro CAG_enh ......... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ..... CAG_enh CMV_imm_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ......... CMV_imm_pro ......... .......... CMV_f

.......... .......... .......... SacI(818) .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_f ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaccgatcg 900

CMV_imm_pro ........ .......... T7_pro .......... ..........

... AflII(908),HindIII(911) ... .......... .......... ..........

901 gtttaaactt aagcttacca tggggggttc tcatcatcat catcatcatg gtatggctag 960

901 caaatttgaa ttcgaatggt accccccaag agtagtagta gtagtagtac cataccgatc 960

.......... .......... .......... 6xHis T7_gene10_leader Xpress_f_pri

.......... .......... .......... .......... .......... ..........

961 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggtacctag 1020

961 gtactgacca cctgtcgttt acccagccct agacatgctg ctactgctat tccatggatc 1020

Xpress_f_pri T7_gene10_leader T7_leader Xpress_EK .. ..........

........ EcoRI(1035) EcoRV(1047) .. NotI(1062) XhoI(1068) ....

1021 gatccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag 1080

1021 ctaggtcaca ccaccttaag acgtctatag gtcgtgtcac cgccggcgag ctcagatctc 1080

.......... .......... .......... .......... .......... ..........

ApaI(1084) .......... .......... .......... .......... ..........

1081 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 1140

1081 ccgggcaaat ttgggcgact agtcggagct gacacggaag atcaacggtc ggtagacaac 1140

.......... .......... ........ BGH_rev_pri bGH_PA_term ........

.......... .......... .......... .......... .......... ..........

1141 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 1200

1141 aaacggggag ggggcacgga aggaactggg accttccacg gtgagggtga caggaaagga 1200

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 1260

1201 ttattttact cctttaacgt agcgtaacag actcatccac agtaagataa gaccccccac 1260

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 1320

1261 cccaccccgt cctgtcgttc cccctcctaa cccttctgtt atcgtccgta cgacccctac 1320

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 1380

1321 gccacccgag ataccgaaga ctccgccttt cttggtcgac cccgagatcc cccatagggg 1380

bGH_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 1440

1381 tgcgcgggac atcgccgcgt aattcgcgcc gcccacacca ccaatgcgcg tcgcactggc 1440

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 1500

1441 gatgtgaacg gtcgcgggat cgcgggcgag gaaagcgaaa gaagggaagg aaagagcggt 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 1560

1501 gcaagcggcc gaaaggggca gttcgagatt tagcccccga gggaaatccc aaggctaaat 1560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 1620

1561 cacgaaatgc cgtggagctg gggttttttg aactaatccc actaccaagt gcatcacccg 1620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 1680

1621 gtagcgggac tatctgccaa aaagcgggaa actgcaacct caggtgcaag aaattatcac 1680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 1740

1681 ctgagaacaa ggtttgacct tgttgtgagt tgggatagag ccagataaga aaactaaata 1740

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 1800

1741 ttccctaaaa cggctaaagc cggataacca attttttact cgactaaatt gtttttaaat 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 1860

1801 tgcgcttaat taagacacct tacacacagt caatcccaca cctttcaggg gtccgagggg 1860

.......... ......... pBABE_3_pri ..... SV40_enh SV40_pro ......

.......... .......... .......... .......... .......... ..........

1861 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 1920

1861 tcgtccgtct tcatacgttt cgtacgtaga gttaatcagt cgttggtcca cacctttcag 1920

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 1980

1921 gggtccgagg ggtcgtccgt cttcatacgt ttcgtacgta gagttaatca gtcgttggta 1980

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 2040

1981 tcagggcggg gattgaggcg ggtagggcgg ggattgaggc gggtcaaggc gggtaagagg 2040

.......... ...... SV40_enh SV40_pro ..... SV40_origin .........

.......... .......... .......... .......... .......... ..........

2041 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 2100

2041 cggggtaccg actgattaaa aaaaataaat acgtctccgg ctccggcgga gacggagact 2100

....... SV40_origin SV40_pro SV40pro_F_pri ....... ..........

.......... .......... .......... StuI(2136) .......... SmaI(2160)

2101 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 2160

2101 cgataaggtc ttcatcactc ctccgaaaaa acctccggat ccgaaaacgt ttttcgaggg 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 2220

2161 ccctcgaaca tataggtaaa agcctagact agttctctgt cctactccta gcaaagcgta 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 2280

2221 ctaacttgtt ctacctaacg tgcgtccaag aggccggcga acccacctct ccgataagcc 2280

.......... .......... ORF2 Neomycin .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 2340

2281 gatactgacc cgtgttgtct gttagccgac gagactacgg cggcacaagg ccgacagtcg 2340

.......... ........ ORF2 Neomycin ........ .......... ..........

NarI(2347) .......... .......... .......... .......... ..........

2341 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 2400

2341 cgtccccgcg ggccaagaaa aacagttctg gctggacagg ccacgggact tacttgacgt 2400

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... MscI(2429) .......... .......... ..........

2401 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 2460

2401 cctgctccgt cgcgccgata gcaccgaccg gtgctgcccg caaggaacgc gtcgacacga 2460

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 2520

2461 gctgcaacag tgacttcgcc cttccctgac cgacgataac ccgcttcacg gccccgtcct 2520

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 2580

2521 agaggacagt agagtggaac gaggacggct ctttcatagg tagtaccgac tacgttacgc 2580

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 2640

2581 cgccgacgta tgcgaactag gccgatggac gggtaagctg gtggttcgct ttgtagcgta 2640

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 2700

2641 gctcgctcgt gcatgagcct accttcggcc agaacagcta gtcctactag acctgcttct 2700

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 2760

2701 cgtagtcccc gagcgcggtc ggcttgacaa gcggtccgag ttccgcgcgt acgggctgcc 2760

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 2820

2761 gctcctagag cagcactggg taccgctacg gacgaacggc ttatagtacc accttttacc 2820

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 2880

2821 ggcgaaaaga cctaagtagc tgacaccggc cgacccacac cgcctggcga tagtcctgta 2880

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 2940

2881 tcgcaaccga tgggcactat aacgacttct cgaaccgccg cttacccgac tggcgaagga 2940

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 3000

2941 gcacgaaatg ccatagcggc gagggctaag cgtcgcgtag cggaagatag cggaagaact 3000

.......... ........ ORF2 Neomycin ........ .......... ..........

.......... ......... BstBI(3029) ......... .......... ..........

3001 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 3060

3001 gctcaagaag actcgccctg agaccccaag ctttactggc tggttcgctg cgggttggac 3060

ORF2 Neomycin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 3120

3061 ggtagtgctc taaagctaag gtggcggcgg aagatacttt ccaacccgaa gccttagcaa 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 3180

3121 aaggccctgc ggccgaccta ctaggaggtc gcgcccctag agtacgacct caagaagcgg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 3240

3181 gtggggttga acaaataacg tcgaatatta ccaatgttta tttcgttatc gtagtgttta 3240

.......... .......... .......... ......... SV40_PA_term .........

.......... .......... .......... .......... .......... ..........

3241 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa a ctcatcaat 3300

3241 aagtgtttat ttcgtaaaaa aagtgacgta agatcaacac caaacaggtt tgagtagtta 3300

.......... ......... SV40_PA_term ......... EBV_r ..........

.......... .......... .......... .......... .......... ..........

3301 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca 3360

3301 catagaatag tacagacata tggcagctgg agatcgatct cgaaccgcat tagtaccagt 3360

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 3420

3361 atcgacaaag gacacacttt aacaataggc gagtgttaag gtgtgttgta tgctcggcct 3420

M13_r .......... M13_pUC_r .......... .......... lac_pro

.......... .......... .......... .......... .......... ..........

3421 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 3480

3421 tcgtatttca catttcggac cccacggatt actcactcga ttgagtgtaa ttaacgcaac 3480

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 3540

3481 gcgagtgacg ggcgaaaggt cagccctttg gacagcacgg tcgacgtaat tacttagccg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 3600

3541 gttgcgcgcc cctctccgcc aaacgcataa cccgcgagaa ggcgaaggag cgagtgactg 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 3660

3601 agcgacgcga gccagcaagc cgacgccgct cgccatagtc gagtgagttt ccgccattat 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 3720

3661 gccaataggt gtcttagtcc cctattgcgt cctttcttgt acactcgttt tccggtcgtt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 3780

3721 ttccggtcct tggcattttt ccggcgcaac gaccgcaaaa aggtatccga ggcgggggga 3780

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3781 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 3840

3781 ctgctcgtag tgtttttagc tgcgagttca gtctccaccg ctttgggctg tcctgatatt 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 3900

3841 tctatggtcc gcaaaggggg accttcgagg gagcacgcga gaggacaagg ctgggacggc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 3960

3901 gaatggccta tggacaggcg gaaagaggga agcccttcgc accgcgaaag agtatcgagt 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 4020

3961 gcgacatcca tagagtcaag ccacatccag caagcgaggt tcgacccgac acacgtgctt 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 4080

4021 ggggggcaag tcgggctggc gacgcggaat aggccattga tagcagaact caggttgggc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 4140

4081 cattctgtgc tgaatagcgg tgaccgtcgt cggtgaccat tgtcctaatc gtctcgctcc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 4200

4141 atacatccgc cacgatgtct caagaacttc accaccggat tgatgccgat gtgatcttct 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 4260

4201 tgtcataaac catagacgcg agacgacttc ggtcaatgga agcctttttc tcaaccatcg 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 4320

4261 agaactaggc cgtttgtttg gtggcgacca tcgccaccaa aaaaacaaac gttcgtcgtc 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 4380

4321 taatgcgcgt ctttttttcc tagagttctt ctaggaaact agaaaagatg ccccagactg 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 4440

4381 cgagtcacct tgcttttgag tgcaattccc taaaaccagt actctaatag tttttcctag 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 4500

4441 aagtggatct aggaaaattt aatttttact tcaaaattta gttagatttc atatatactc 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 4560

4501 atttgaacca gactgtcaat ggttacgaat tagtcactcc gtggatagag tcgctagaca 4560

.......... .......... ....... ORF3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4561 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 4620

4561 gataaagcaa gtaggtatca acggactgag gggcagcaca tctattgatg ctatgccctc 4620

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 4680

4621 ccgaatggta gaccggggtc acgacgttac tatggcgctc tgggtgcgag tggccgaggt 4680

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 4740

4681 ctaaatagtc gttatttggt cggtcggcct tcccggctcg cgtcttcacc aggacgttga 4740

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 4800

4741 aataggcgga ggtaggtcag ataattaaca acggcccttc gatctcattc atcaagcggt 4800

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 4860

4801 caattatcaa acgcgttgca acaacggtaa cgatgtccgt agcaccacag tgcgagcagc 4860

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 4920

4861 aaaccatacc gaagtaagtc gaggccaagg gttgctagtt ccgctcaatg tactaggggg 4920

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 4980

4921 tacaacacgt tttttcgcca atcgaggaag ccaggaggct agcaacagtc ttcattcaac 4980

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 5040

4981 cggcgtcaca atagtgagta ccaataccgt cgtgacgtat taagagaatg acagtacggt 5040

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 5100

5041 aggcattcta cgaaaagaca ctgaccactc atgagttggt tcagtaagac tcttatcaca 5100

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 5160

5101 tacgccgctg gctcaacgag aacgggccgc agttatgccc tattatggcg cggtgtatcg 5160

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 5220

5161 tcttgaaatt ttcacgagta gtaacctttt gcaagaagcc ccgcttttga gagttcctag 5220

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 5280

5221 aatggcgaca actctaggtc aagctacatt gggtgagcac gtgggttgac tagaagtcgt 5280

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 5340

5281 agaaaatgaa agtggtcgca aagacccact cgtttttgtc cttccgtttt acggcgtttt 5340

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 5400

5341 ttcccttatt cccgctgtgc ctttacaact tatgagtatg agaaggaaaa agttataata 5400

....... ORF3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 5460

5401 acttcgtaaa tagtcccaat aacagagtac tcgcctatgt ataaacttac ataaatcttt 5460

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc...... 5514

5461 ttatttgttt atccccaagg cgcgtgtaaa ggggcttttc acggtggact gcag...... 5514

.......... .......... .......... .......... .......... ..........